BuceA.00010.g
Short chain dehydrogenase
| CENTER ID: | BuceA.00010.g |
| ORGANISM: | Burkholderia cenocepacia J2315 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.00010.g.B1.GE36335 | Full length( BuceA.00010.g ) | 1 | 246 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
5U2W
DEPOSITED: 12/1/2016
DETERMINATION: XRay
CLONE: BuceA.00010.g.B1.GE36335
PROTEIN: BuceA.00010.g.B1.PW37236
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_129240 |
| PATRIC ID: | fig|216591.5.peg.2579 |
| RefSeq: | YP_002231588.1 |
| UniProt: | B4E730 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNRLQGKRAL VTGGSRGIGA AIAKRLAADG ADVAITYEKS AERAQAVVAG IEALGRRAIA IQADSADPVA VRNAVDRVAE AFGGLDILVN NAGIFRAGSL DDLTLDDIDA TLNVNVRAVI VASQAAARHL GEGGRIVSTG SCLATRVPDA GMSLYAASKA ALIGWTQGLA RDLGPRGITV NIVHPGSTDT DMNPADGAHA DAQRSRMAIQ QYGKADDVAA LVAFVVGPEG RSINGTGLTI DGGANA
NT Sequence
ATGAACCGAC TTCAGGGCAA GCGTGCACTC GTCACGGGCG GCAGCCGCGG CATCGGCGCG GCGATCGCGA AACGGCTGGC GGCCGACGGC GCGGACGTCG CGATCACGTA TGAAAAATCG GCCGAGCGGG CGCAGGCCGT GGTCGCCGGC ATCGAGGCGC TGGGCCGGCG CGCCATCGCG ATCCAGGCCG ACAGCGCCGA TCCGGTCGCC GTGCGCAACG CGGTCGATCG TGTCGCGGAG GCATTCGGCG GACTCGACAT CCTCGTCAAC AACGCGGGGA TTTTCCGGGC CGGATCGCTC GACGACCTCA CGCTCGACGA CATCGACGCG ACGCTGAACG TGAACGTGCG CGCGGTGATC GTCGCGTCGC AGGCGGCGGC GCGGCATCTC GGCGAGGGCG GGCGCATCGT GTCGACCGGC AGTTGCCTCG CGACGCGCGT GCCCGATGCG GGGATGAGCC TCTATGCGGC GAGCAAGGCC GCGCTGATCG GCTGGACGCA GGGGCTCGCG CGCGATCTCG GCCCGCGCGG CATCACGGTC AACATCGTGC ATCCGGGGTC GACCGATACC GACATGAACC CGGCCGATGG CGCGCATGCC GATGCGCAGC GTTCGCGGAT GGCGATTCAG CAGTACGGCA AGGCCGACGA CGTGGCCGCG CTCGTGGCGT TCGTCGTCGG ACCGGAAGGG CGGTCGATCA ACGGGACCGG ATTGACGATC GACGGCGGCG CGAACGCG
Details for BuceA.00010.g.B1.GE36335
| HARVESTED ON: | 10/23/2012 |
| SEQUENCED ON: | 10/26/2012 |
| EXPECTED MW: | 25kDa |
| OBSERVED MW: | 26kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMN RLQGKRALVT GGSRGIGAAI AKRLAADGAD VAITYEKSAE RAQAVVAGIE ALGRRAIAIQ ADSADPVAVR NAVDRVAEAF GGLDILVNNA GIFRAGSLDD LTLDDIDATL NVNVRAVIVA SQAAARHLGE GGRIVSTGSC LATRVPDAGM SLYAASKAAL IGWTQGLARD LGPRGITVNI VHPGSTDTDM NPADGAHADA QRSRMAIQQY GKADDVAALV AFVVGPEGRS INGTGLTIDG GANA
Validated NT Sequence
atcttactca cttacgcgtt cgcgccgccg tcgatcgtca atccggtccc gttgatcgac cgcccttccg gtccgacgac gaacgccacg agcgcggcca cgtcgtcggc cttgccgtac tgctgaatcg ccatccgcga acgctgcgca tcggcatgcg cgccatcggc cgggttcatg tcggtatcgg tcgaccccgg atgcacgatg ttgaccgtga tgccgcgcgg gccgagatcg cgcgcgagcc cctgcgtcca gccgatcagc gcggccttgc tcgccgcata gaggctcatc cccgcatcgg gcacgcgcgt cgcgaggcaa ctgccggtcg acacgatgcg cccgccctcg ccgagatgcc gcgccgccgc ctgcgacgcg acgatcaccg cgcgcacgtt cacgttcagc gtcgcgtcga tgtcgtcgag cgtgaggtcg tcgagcgatc cggcccggaa aatccccgcg ttgttgacga ggatgtcgag tccgccgaat gcctccgcga cacgatcgac cgcgttgcgc acggcgaccg gatcggcgct gtcggcctgg atcgcgatgg cgcgccggcc cagcgcctcg atgccggcga ccacggcctg cgcccgctcg gccgattttt catacgtgat cgcgacgtcc gcgccgtcgg ccgccagccg tttcgcgatc gccgcgccga tgccgcggct gccgcccgtg acgagtgcac gcttgccctg aagtcggttc atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHMN RLQGKRALVT GGSRGIGAAI AKRLAADGAD VAITYEKSAE RAQAVVAGIE ALGRRAIAIQ ADSADPVAVR NAVDRVAEAF GGLDILVNNA GIFRAGSLDD LTLDDIDATL NVNVRAVIVA SQAAARHLGE GGRIVSTGSC LATRVPDAGM SLYAASKAAL IGWTQGLARD LGPRGITVNI VHPGSTDTDM NPADGAHADA QRSRMAIQQY GKADDVAALV AFVVGPEGRS INGTGLTIDG GANA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacc gacttcaggg caagcgtgca ctcgtcacgg gcggcagccg cggcatcggc gcggcgatcg cgaaacggct ggcggccgac ggcgcggacg tcgcgatcac gtatgaaaaa tcggccgagc gggcgcaggc cgtggtcgcc ggcatcgagg cgctgggccg gcgcgccatc gcgatccagg ccgacagcgc cgatccggtc gccgtgcgca acgcggtcga tcgtgtcgcg gaggcattcg gcggactcga catcctcgtc aacaacgcgg ggattttccg ggccggatcg ctcgacgacc tcacgctcga cgacatcgac gcgacgctga acgtgaacgt gcgcgcggtg atcgtcgcgt cgcaggcggc ggcgcggcat ctcggcgagg gcgggcgcat cgtgtcgacc ggcagttgcc tcgcgacgcg cgtgcccgat gcggggatga gcctctatgc ggcgagcaag gccgcgctga tcggctggac gcaggggctc gcgcgcgatc tcggcccgcg cggcatcacg gtcaacatcg tgcatccggg gtcgaccgat accgacatga acccggccga tggcgcgcat gccgatgcgc agcgttcgcg gatggcgatt cagcagtacg gcaaggccga cgacgtggcc gcgctcgtgg cgttcgtcgt cggaccggaa gggcggtcga tcaacgggac cggattgacg atcgacggcg gcgcgaacgc gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat