BuceA.00010.f
Putative glucose 1-dehydrogenase
| CENTER ID: | BuceA.00010.f |
| ORGANISM: | Burkholderia cenocepacia J2315 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.00010.f.B1.GE36334 | Full length( BuceA.00010.f ) | 1 | 248 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.00010.f.B1.PW37272 | Full length( BuceA.00010.f ) | 1 | 248 |
Structures
4MOW
DEPOSITED: 9/12/2013
DETERMINATION: XRay
CLONE: BuceA.00010.f.B1.GE36334
PROTEIN: BuceA.00010.f.B1.PW37272
7V0H
DEPOSITED: 5/10/2022
DETERMINATION: XRay
CLONE: BuceA.00010.f.B1.GE36334
PROTEIN: BuceA.00010.f.B1.PW37272
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_129240 |
| PATRIC ID: | fig|216591.5.peg.1481 |
| RefSeq: | YP_002230556.1 |
| UniProt: | B4E6Z1 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSKLAGKVAI VTGASKGIGA AIAKALADEG AAVVVNYASS KAGADAVVSA ITEAGGRAVA VGGDVSKAAD AQRIVDTAIE TYGRLDVLVN NSGVYEFAPI EAITEEHYRR QFDTNVFGVL LTTQAAVKHL GEGASIINIS SVVTSITPPA SAVYSGTKGA VDAITGVLAL ELGPRKIRVN AINPGMIVTE GTHSAGIIGS DLEAQVLGQT PLGRLGEPND IASVAVFLAS DDARWMTGEH LVVSGGLN
NT Sequence
ATGAGCAAGC TGGCAGGCAA GGTCGCGATC GTCACGGGCG CATCGAAGGG CATCGGCGCG GCAATCGCGA AGGCACTGGC CGACGAAGGC GCGGCGGTCG TCGTCAACTA CGCGAGTAGC AAGGCAGGCG CGGACGCGGT CGTCAGCGCG ATCACCGAAG CGGGCGGCCG CGCGGTCGCG GTCGGCGGCG ACGTGTCGAA GGCCGCCGAC GCGCAACGCA TCGTCGATAC GGCGATCGAA ACGTATGGCC GTCTCGACGT GCTCGTCAAC AATTCGGGCG TGTACGAATT CGCGCCGATC GAAGCGATCA CCGAGGAACA CTATCGCCGG CAGTTCGACA CGAACGTGTT CGGCGTGCTG CTGACCACGC AGGCGGCCGT CAAGCATCTC GGCGAAGGCG CGAGCATCAT CAACATCAGT TCGGTCGTGA CCAGCATCAC GCCGCCGGCC AGCGCCGTGT ACAGCGGCAC GAAGGGTGCG GTCGACGCGA TCACCGGCGT GCTCGCGCTC GAACTCGGCC CGCGCAAGAT CCGCGTGAAC GCGATCAACC CGGGGATGAT CGTGACCGAG GGCACGCACA GCGCGGGCAT CATCGGCTCC GATCTGGAAG CGCAGGTGCT CGGCCAGACG CCGCTCGGCC GCCTCGGCGA GCCGAACGAC ATCGCGTCGG TCGCCGTGTT CCTCGCGTCG GACGATGCGC GCTGGATGAC TGGCGAGCAC CTCGTCGTGA GCGGCGGGTT GAAC
Details for BuceA.00010.f.B1.GE36334
| HARVESTED ON: | 10/23/2012 |
| SEQUENCED ON: | 10/26/2012 |
| EXPECTED MW: | 26kDa |
| OBSERVED MW: | 26kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMS KLAGKVAIVT GASKGIGAAI AKALADEGAA VVVNYASSKA GADAVVSAIT EAGGRAVAVG GDVSKAADAQ RIVDTAIETY GRLDVLVNNS GVYEFAPIEA ITEEHYRRQF DTNVFGVLLT TQAAVKHLGE GASIINISSV VTSITPPASA VYSGTKGAVD AITGVLALEL GPRKIRVNAI NPGMIVTEGT HSAGIIGSDL EAQVLGQTPL GRLGEPNDIA SVAVFLASDD ARWMTGEHLV VSGGLN
Validated NT Sequence
atcttactca cttagttcaa cccgccgctc acgacgaggt gctcgccagt catccagcgc gcatcgtccg acgcgaggaa cacggcgacc gacgcgatgt cgttcggctc gccgaggcgg ccgagcggcg tctggccgag cacctgcgct tccagatcgg agccgatgat gcccgcgctg tgcgtgccct cggtcacgat catccccggg ttgatcgcgt tcacgcggat cttgcgcggg ccgagttcga gcgcgagcac gccggtgatc gcgtcgaccg cacccttcgt gccgctgtac acggcgctgg ccggcggcgt gatgctggtc acgaccgaac tgatgttgat gatgctcgcg ccttcgccga gatgcttgac ggccgcctgc gtggtcagca gcacgccgaa cacgttcgtg tcgaactgcc ggcgatagtg ttcctcggtg atcgcttcga tcggcgcgaa ttcgtacacg cccgaattgt tgacgagcac gtcgagacgg ccatacgttt cgatcgccgt atcgacgatg cgttgcgcgt cggcggcctt cgacacgtcg ccgccgaccg cgaccgcgcg gccgcccgct tcggtgatcg cgctgacgac cgcgtccgcg cctgccttgc tactcgcgta gttgacgacg accgccgcgc cttcgtcggc cagtgccttc gcgattgccg cgccgatgcc cttcgatgcg cccgtgacga tcgcgacctt gcctgccagc ttgctcatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHMS KLAGKVAIVT GASKGIGAAI AKALADEGAA VVVNYASSKA GADAVVSAIT EAGGRAVAVG GDVSKAADAQ RIVDTAIETY GRLDVLVNNS GVYEFAPIEA ITEEHYRRQF DTNVFGVLLT TQAAVKHLGE GASIINISSV VTSITPPASA VYSGTKGAVD AITGVLALEL GPRKIRVNAI NPGMIVTEGT HSAGIIGSDL EAQVLGQTPL GRLGEPNDIA SVAVFLASDD ARWMTGEHLV VSGGLN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagca agctggcagg caaggtcgcg atcgtcacgg gcgcatcgaa gggcatcggc gcggcaatcg cgaaggcact ggccgacgaa ggcgcggcgg tcgtcgtcaa ctacgcgagt agcaaggcag gcgcggacgc ggtcgtcagc gcgatcaccg aagcgggcgg ccgcgcggtc gcggtcggcg gcgacgtgtc gaaggccgcc gacgcgcaac gcatcgtcga tacggcgatc gaaacgtatg gccgtctcga cgtgctcgtc aacaattcgg gcgtgtacga attcgcgccg atcgaagcga tcaccgagga acactatcgc cggcagttcg acacgaacgt gttcggcgtg ctgctgacca cgcaggcggc cgtcaagcat ctcggcgaag gcgcgagcat catcaacatc agttcggtcg tgaccagcat cacgccgccg gccagcgccg tgtacagcgg cacgaagggt gcggtcgacg cgatcaccgg cgtgctcgcg ctcgaactcg gcccgcgcaa gatccgcgtg aacgcgatca acccggggat gatcgtgacc gagggcacgc acagcgcggg catcatcggc tccgatctgg aagcgcaggt gctcggccag acgccgctcg gccgcctcgg cgagccgaac gacatcgcgt cggtcgccgt gttcctcgcg tcggacgatg cgcgctggat gactggcgag cacctcgtcg tgagcggcgg gttgaactga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for BuceA.00010.f.B1.PW37272
| PURIFICATION DATe: | 4/1/2013 |
| CONCENTRATION: | 29.8mg/ml |
| OBSERVED MW: | 27kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 20 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS KLAGKVAIVT GASKGIGAAI AKALADEGAA VVVNYASSKA GADAVVSAIT EAGGRAVAVG GDVSKAADAQ RIVDTAIETY GRLDVLVNNS GVYEFAPIEA ITEEHYRRQF DTNVFGVLLT TQAAVKHLGE GASIINISSV VTSITPPASA VYSGTKGAVD AITGVLALEL GPRKIRVNAI NPGMIVTEGT HSAGIIGSDL EAQVLGQTPL GRLGEPNDIA SVAVFLASDD ARWMTGEHLV VSGGLN
Validated NT Sequence
atcttactca cttagttcaa cccgccgctc acgacgaggt gctcgccagt catccagcgc gcatcgtccg acgcgaggaa cacggcgacc gacgcgatgt cgttcggctc gccgaggcgg ccgagcggcg tctggccgag cacctgcgct tccagatcgg agccgatgat gcccgcgctg tgcgtgccct cggtcacgat catccccggg ttgatcgcgt tcacgcggat cttgcgcggg ccgagttcga gcgcgagcac gccggtgatc gcgtcgaccg cacccttcgt gccgctgtac acggcgctgg ccggcggcgt gatgctggtc acgaccgaac tgatgttgat gatgctcgcg ccttcgccga gatgcttgac ggccgcctgc gtggtcagca gcacgccgaa cacgttcgtg tcgaactgcc ggcgatagtg ttcctcggtg atcgcttcga tcggcgcgaa ttcgtacacg cccgaattgt tgacgagcac gtcgagacgg ccatacgttt cgatcgccgt atcgacgatg cgttgcgcgt cggcggcctt cgacacgtcg ccgccgaccg cgaccgcgcg gccgcccgct tcggtgatcg cgctgacgac cgcgtccgcg cctgccttgc tactcgcgta gttgacgacg accgccgcgc cttcgtcggc cagtgccttc gcgattgccg cgccgatgcc cttcgatgcg cccgtgacga tcgcgacctt gcctgccagc ttgctcatat ggtggtggtg gtggtgagcc at
Expressed Protein Sequence
MAHHHHHHMS KLAGKVAIVT GASKGIGAAI AKALADEGAA VVVNYASSKA GADAVVSAIT EAGGRAVAVG GDVSKAADAQ RIVDTAIETY GRLDVLVNNS GVYEFAPIEA ITEEHYRRQF DTNVFGVLLT TQAAVKHLGE GASIINISSV VTSITPPASA VYSGTKGAVD AITGVLALEL GPRKIRVNAI NPGMIVTEGT HSAGIIGSDL EAQVLGQTPL GRLGEPNDIA SVAVFLASDD ARWMTGEHLV VSGGLN
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCA AGCTGGCAGG CAAGGTCGCG ATCGTCACGG GCGCATCGAA GGGCATCGGC GCGGCAATCG CGAAGGCACT GGCCGACGAA GGCGCGGCGG TCGTCGTCAA CTACGCGAGT AGCAAGGCAG GCGCGGACGC GGTCGTCAGC GCGATCACCG AAGCGGGCGG CCGCGCGGTC GCGGTCGGCG GCGACGTGTC GAAGGCCGCC GACGCGCAAC GCATCGTCGA TACGGCGATC GAAACGTATG GCCGTCTCGA CGTGCTCGTC AACAATTCGG GCGTGTACGA ATTCGCGCCG ATCGAAGCGA TCACCGAGGA ACACTATCGC CGGCAGTTCG ACACGAACGT GTTCGGCGTG CTGCTGACCA CGCAGGCGGC CGTCAAGCAT CTCGGCGAAG GCGCGAGCAT CATCAACATC AGTTCGGTCG TGACCAGCAT CACGCCGCCG GCCAGCGCCG TGTACAGCGG CACGAAGGGT GCGGTCGACG CGATCACCGG CGTGCTCGCG CTCGAACTCG GCCCGCGCAA GATCCGCGTG AACGCGATCA ACCCGGGGAT GATCGTGACC GAGGGCACGC ACAGCGCGGG CATCATCGGC TCCGATCTGG AAGCGCAGGT GCTCGGCCAG ACGCCGCTCG GCCGCCTCGG CGAGCCGAAC GACATCGCGT CGGTCGCCGT GTTCCTCGCG TCGGACGATG CGCGCTGGAT GACTGGCGAG CACCTCGTCG TGAGCGGCGG GTTGAACTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT