SSGCID: Target Page

BuceA.00010.a

Putative dehydrogenase

CENTER ID:	BuceA.00010.a
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	in PDB
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00010.a.B1.GE36329	Full length( BuceA.00010.a )		1	253

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Structures

4JIG

DEPOSITED: 3/6/2013

DETERMINATION: XRay

Diffraction Image

CLONE: BuceA.00010.a.B1.GE36329

PROTEIN: BuceA.00010.a.B1.PS01733

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.2471
RefSeq:	YP_002231493.1
UniProt:	B4E5P7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTASSDRKAV LITGASRGIG RATAVLAAER GWDVGINYAR DAAAAELTAQ AVRDAGGRAC IVAGDVANEA DVVAMFDTVA AAFGRLDALV NNAGIVAPSM PLADMPVDRL RRMFDTNVLG AYLCAREAAR RLSTDRGGRG GAIVNVSSIA SRLGSPNEYV DYAGSKGAVD SLTIGLAKEL GPHGVRVNAV RPGLIETDIH ASGGQPGRAA RLGAQTPLGR AGEAQEVAEA IVWLLGDTAS YTTGALLDVG GGR

NT Sequence

ATGACCGCAT CGTCCGACCG CAAGGCCGTC CTCATCACCG GCGCGAGCCG CGGCATCGGC CGCGCGACCG CCGTGCTCGC GGCCGAACGC GGCTGGGACG TCGGCATCAA CTACGCGCGC GACGCGGCGG CGGCCGAACT GACCGCGCAG GCCGTGCGCG ACGCGGGCGG CCGCGCGTGC ATCGTCGCGG GCGATGTCGC GAACGAGGCG GACGTCGTCG CGATGTTCGA TACCGTTGCG GCCGCGTTCG GACGCCTCGA CGCGCTCGTC AACAACGCGG GCATCGTCGC GCCGTCGATG CCGCTCGCCG ACATGCCGGT CGACCGGTTG CGGCGGATGT TCGATACCAA CGTGCTCGGC GCCTACCTGT GCGCGCGCGA AGCCGCGCGC CGGCTGTCGA CCGACCGCGG CGGCCGCGGC GGCGCGATCG TCAACGTGTC GTCGATCGCA TCGCGGCTCG GCTCGCCGAA CGAGTACGTC GACTACGCGG GCTCGAAAGG CGCGGTCGAT TCGCTGACGA TCGGCCTCGC CAAGGAACTC GGCCCGCACG GCGTGCGCGT CAACGCGGTA CGCCCCGGCC TGATCGAGAC CGACATTCAT GCAAGCGGCG GCCAGCCGGG CCGTGCGGCC CGCCTCGGCG CGCAAACGCC GCTCGGCCGC GCGGGCGAAG CGCAGGAGGT CGCCGAGGCG ATCGTCTGGC TGCTCGGCGA TACGGCCTCC TACACGACGG GCGCCCTGCT CGACGTCGGC GGCGGCCGC

Details for BuceA.00010.a.B1.GE36329

HARVESTED ON:	10/23/2012
SEQUENCED ON:	10/26/2012
EXPECTED MW:	26kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	96
PERCENT COVERAGE:	95

Validated AA Sequence

Validated NT Sequence

acgtcgagca gggcgcccgt cgtgtaggag gccngtatcg ccgagcagcc agacgatcgc ctnggcgacc tcntgcgctt cgcccgcgcg gccgagcggc gtttgcgcgc cgaggcgggc cgcacggccc ggctggccgc cgcttgcatg aatgtcggtc tcgatcaggc cggggcgtac cgcgttgacg cgcacgccgt gcgggccgag ttccttggcg aggccgatcg tcagcgaatc gaccgcgcct ttcgagcccg cgtagtcgac gtactcgttc ggcgagccga gccgcgatgc gatcgacgac acgttgacga tcgcgccgcc gcggccgccg cggtcggtcg acagccggcg cgcggcttcg cgcgcgcaca ggtaggcgcc gagcacgttg gtatcgaaca tccgccgcaa ccggtcgacc ggcatgtcgg cgagcggcat cgacggcgcg acgatgcccg cgttgttgac gagcgcgtcg aggcgtccga acgcggccgc aacggtatcg aacatcgcga cgacgtccgc ctcgttcgcg acatcgcccg cgacgatgca cgcgcggccg cccgcgtcgc gcacggcctg cgcggtcagt tcggccgccg ccgcgtcgcg cgcgtagttg atgccgacgt cccagccgcg ttcggccgcg agcacggcgg tcgcgcggcc gatgccgcgg ctcgcgccgg tgatgaggac ggccttgcgg tcggacgatg cggtcatatg gtggtggtgg tggtgagcc

Expected Protein Sequence

MAHHHHHHMT ASSDRKAVLI TGASRGIGRA TAVLAAERGW DVGINYARDA AAAELTAQAV RDAGGRACIV AGDVANEADV VAMFDTVAAA FGRLDALVNN AGIVAPSMPL ADMPVDRLRR MFDTNVLGAY LCAREAARRL STDRGGRGGA IVNVSSIASR LGSPNEYVDY AGSKGAVDSL TIGLAKELGP HGVRVNAVRP GLIETDIHAS GGQPGRAARL GAQTPLGRAG EAQEVAEAIV WLLGDTASYT TGALLDVGGG R

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaccg catcgtccga ccgcaaggcc gtcctcatca ccggcgcgag ccgcggcatc ggccgcgcga ccgccgtgct cgcggccgaa cgcggctggg acgtcggcat caactacgcg cgcgacgcgg cggcggccga actgaccgcg caggccgtgc gcgacgcggg cggccgcgcg tgcatcgtcg cgggcgatgt cgcgaacgag gcggacgtcg tcgcgatgtt cgataccgtt gcggccgcgt tcggacgcct cgacgcgctc gtcaacaacg cgggcatcgt cgcgccgtcg atgccgctcg ccgacatgcc ggtcgaccgg ttgcggcgga tgttcgatac caacgtgctc ggcgcctacc tgtgcgcgcg cgaagccgcg cgccggctgt cgaccgaccg cggcggccgc ggcggcgcga tcgtcaacgt gtcgtcgatc gcatcgcggc tcggctcgcc gaacgagtac gtcgactacg cgggctcgaa aggcgcggtc gattcgctga cgatcggcct cgccaaggaa ctcggcccgc acggcgtgcg cgtcaacgcg gtacgccccg gcctgatcga gaccgacatt catgcaagcg gcggccagcc gggccgtgcg gcccgcctcg gcgcgcaaac gccgctcggc cgcgcgggcg aagcgcagga ggtcgccgag gcgatcgtct ggctgctcgg cgatacggcc tcctacacga cgggcgccct gctcgacgtc ggcggcggcc gctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat