SSGCID: Target Page

BuceA.00009.a

Putative kynureninase

CENTER ID:	BuceA.00009.a
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00009.a.B1.GE36328	Full length( BuceA.00009.a )		1	417

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00009.a.B1.PS01723	Full length( BuceA.00009.a )		1	417

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.2890
RefSeq:	YP_002231893.1
UniProt:	B4E9J0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIKTREDALA LDRDDPLAPL RDQFSLPDGV IYLDGNSLGA QPRASAARAQ QVIGAEWGEG LIRSWNTAGW FALPRRLGDK LATLIGGAPG ETVVTDTISI NLFKLLSAML RHQAERAPER RVIVSERSNF PTDLYIAQGL IDQLGGGYTL RLIDDPADLP GALGADTAVA MITHVNYRTG YMHDMPAVTQ LVHDAGALML WDLAHSAGAV PVDLNGARAD GAVGCTYKYL NGGPGSPAFV WVPQRHLAHF SQPLSGWWGH RAPFAMQPGF APDPGIARFL CGTQPIVSMS MVECGLDVFL QTDMDAIRRK SLALTDAFIA LVEARCAGLS LKLVTPRAHH QRGSQASFEH PHGYEVMQAL IARGVIGDYR EPYVLRFGFT PLYTRFADVW DAVETLRDIL ATDAWKAPEF AERGAVT

NT Sequence

ATGATCAAGA CCCGTGAAGA CGCGCTCGCG CTCGACCGCG ACGACCCGCT GGCCCCGCTG CGCGACCAGT TCTCGCTGCC GGACGGCGTG ATCTACCTCG ACGGCAACTC GCTCGGCGCG CAGCCACGTG CATCGGCCGC CCGCGCGCAG CAGGTGATCG GCGCCGAATG GGGCGAAGGC CTGATCCGCA GCTGGAACAC GGCCGGCTGG TTCGCGCTGC CGCGCCGCCT CGGCGACAAG CTCGCGACGC TGATCGGCGG CGCGCCCGGC GAGACGGTCG TCACCGACAC CATCTCGATC AACCTGTTCA AGCTGCTGTC GGCGATGCTG CGCCACCAGG CCGAGCGTGC GCCCGAGCGC CGGGTGATCG TGTCGGAGCG CTCGAACTTC CCGACCGACC TGTACATCGC GCAGGGGCTG ATCGACCAGC TCGGCGGCGG CTACACGCTG CGCCTGATCG ACGATCCGGC CGACCTGCCC GGCGCGCTCG GCGCGGACAC GGCCGTCGCG ATGATCACGC ACGTGAACTA CCGCACCGGC TACATGCACG ACATGCCGGC CGTCACACAG CTGGTGCACG ACGCGGGCGC GCTGATGCTG TGGGATCTCG CGCACTCGGC CGGCGCAGTG CCGGTCGACC TGAACGGCGC GCGCGCGGAC GGTGCGGTCG GCTGCACGTA CAAGTACCTG AACGGCGGCC CCGGCTCGCC CGCGTTCGTG TGGGTGCCGC AACGCCATCT CGCGCATTTC TCGCAGCCGC TGTCCGGCTG GTGGGGCCAC CGCGCGCCGT TCGCGATGCA GCCGGGCTTC GCGCCCGATC CGGGCATCGC GCGCTTCCTG TGCGGCACGC AGCCGATCGT GTCGATGTCG ATGGTCGAAT GCGGGCTCGA CGTGTTCCTG CAGACCGACA TGGACGCGAT CCGCCGCAAG TCGCTCGCGT TGACCGATGC CTTCATCGCG CTCGTCGAAG CGCGCTGCGC GGGCCTGTCG CTGAAGCTCG TCACGCCGCG TGCGCATCAC CAGCGCGGCT CGCAGGCGAG CTTCGAGCAT CCGCACGGCT ACGAAGTGAT GCAGGCGCTG ATCGCGCGCG GCGTGATCGG CGACTACCGC GAGCCGTACG TGCTGCGCTT CGGCTTCACG CCGCTCTATA CGCGCTTCGC CGACGTGTGG GATGCGGTCG AGACGCTGCG CGACATCCTC GCCACCGACG CGTGGAAGGC GCCCGAGTTC GCCGAACGCG GCGCGGTGAC C

Details for BuceA.00009.a.B1.GE36328

HARVESTED ON:	10/23/2012
SEQUENCED ON:	10/26/2012
EXPECTED MW:	46kDa
OBSERVED MW:	43kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	79
PERCENT COVERAGE:	90

Validated AA Sequence

MAHHHHHHMI KTREDALALD RDDPLAPLRD QFSLPDGVIY LDGNSLGAQP RASAARAQQV IGAEWGEGLI RSWNTAGWFA LPRRLGDKLA TLIGGAPGET VVTDTISINL FKLLSAMLRH QAERAPERRV IVSERSNFPT DLYIAQGLID QLGGGYTLRL IDDPADLPGA LGADTAVAMI THVNYRTGYM HDMPAVTQLV HDAGALMLWD LAHSAGAVPV DLNGARADGA VGCTYKYLNG GPGSPAFVWV PQRHLAHFSQ PLSGWWGHRA PFAMQPGFAP DPGIARFLCG TQPIVSMSMV EXXXDVXXXX DXXXIPXXXA LXXXXIXXXX XXXXXXXXXX XXXXXXXXXX XIXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXX

Validated NT Sequence

ttttnnttnn ctttaagaag gaganatacc atggctcacc accaccacca ccatatgatc aagacccgtg aagacgcgct cgcgctcgac cgcgacgacc cgctggcccc gctgcgcgac cagttctcgc tgccggacgg cgtgatctac ctcgacggca actcgctcgg cgcgcagcca cgtgcatcgg ccgcccgcgc gcagcaggtg atcggcgccg aatggggcga aggcctgatc cgcagctgga acacggccgg ctggttcgcg ctgccgcgcc gcctcggcga caagctcgcg acgctgatcg gcggcgcgcc cggcgagacg gtcgtcaccg acaccatctc gatcaacctg ttcaagctgc tgtcggcgat gctgcgccac caggccgagc gtgcgcccga gcgccgggtg atcgtgtcgg agcgctcgaa cttcccgacc gacctgtaca tcgcgcaggg gctgatcgac cagctcggcg gcggctacac gctgcgcctg atcgacgatc cggccgacct gcccggcgcg ctcggcgcgg acacggccgt cgcgatgatc acgcacgtga actaccgcac cggctacatg cacgacatgc cggccgtcac acagctggtg cacgacgcgg gcgcgctgat gctgtgggat ctcgcgcact cggccggcgc agtgccggtc gacctgaacg gcgcgcgcgc ggacggtgcg gtcggctgca cgtacaagta cctgaacggc ggccccggct cgcccgcgtt cgtgtgggtg ccgcaacgcc atctcgcgca tttctcgcag ccgctgtccg gctggtgggg ccaccgcgcg ccgttcgcga tgcagccggg nttcgcgccc gatccgggca tcgcgcgctt cctgtgcggc acgcagccga tcgtgtcgat gtcgatggtc gaatgnnggn ncgacgtgnt nntncnnann gacntngnnn nnatcccnna ntnnntcgcg ttgancnatg nnnncatcnn nntngncnat nnnnnggnnn nnnnntnnnn tnnnnannnn ncnnnnncat nnncngnnnn nnngnnnnnn nncatccnnn nnnnnnnnnn nnnnncnnnn nnnnncnnnn nnnnnnnnnn nnnnnnnnnc nnnnnnnnnn nnnnnnnnnn nnnncnnnnn nnnnntncna

Expected Protein Sequence

MAHHHHHHMI KTREDALALD RDDPLAPLRD QFSLPDGVIY LDGNSLGAQP RASAARAQQV IGAEWGEGLI RSWNTAGWFA LPRRLGDKLA TLIGGAPGET VVTDTISINL FKLLSAMLRH QAERAPERRV IVSERSNFPT DLYIAQGLID QLGGGYTLRL IDDPADLPGA LGADTAVAMI THVNYRTGYM HDMPAVTQLV HDAGALMLWD LAHSAGAVPV DLNGARADGA VGCTYKYLNG GPGSPAFVWV PQRHLAHFSQ PLSGWWGHRA PFAMQPGFAP DPGIARFLCG TQPIVSMSMV ECGLDVFLQT DMDAIRRKSL ALTDAFIALV EARCAGLSLK LVTPRAHHQR GSQASFEHPH GYEVMQALIA RGVIGDYREP YVLRFGFTPL YTRFADVWDA VETLRDILAT DAWKAPEFAE RGAVT

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatca agacccgtga agacgcgctc gcgctcgacc gcgacgaccc gctggccccg ctgcgcgacc agttctcgct gccggacggc gtgatctacc tcgacggcaa ctcgctcggc gcgcagccac gtgcatcggc cgcccgcgcg cagcaggtga tcggcgccga atggggcgaa ggcctgatcc gcagctggaa cacggccggc tggttcgcgc tgccgcgccg cctcggcgac aagctcgcga cgctgatcgg cggcgcgccc ggcgagacgg tcgtcaccga caccatctcg atcaacctgt tcaagctgct gtcggcgatg ctgcgccacc aggccgagcg tgcgcccgag cgccgggtga tcgtgtcgga gcgctcgaac ttcccgaccg acctgtacat cgcgcagggg ctgatcgacc agctcggcgg cggctacacg ctgcgcctga tcgacgatcc ggccgacctg cccggcgcgc tcggcgcgga cacggccgtc gcgatgatca cgcacgtgaa ctaccgcacc ggctacatgc acgacatgcc ggccgtcaca cagctggtgc acgacgcggg cgcgctgatg ctgtgggatc tcgcgcactc ggccggcgca gtgccggtcg acctgaacgg cgcgcgcgcg gacggtgcgg tcggctgcac gtacaagtac ctgaacggcg gccccggctc gcccgcgttc gtgtgggtgc cgcaacgcca tctcgcgcat ttctcgcagc cgctgtccgg ctggtggggc caccgcgcgc cgttcgcgat gcagccgggc ttcgcgcccg atccgggcat cgcgcgcttc ctgtgcggca cgcagccgat cgtgtcgatg tcgatggtcg aatgcgggct cgacgtgttc ctgcagaccg acatggacgc gatccgccgc aagtcgctcg cgttgaccga tgccttcatc gcgctcgtcg aagcgcgctg cgcgggcctg tcgctgaagc tcgtcacgcc gcgtgcgcat caccagcgcg gctcgcaggc gagcttcgag catccgcacg gctacgaagt gatgcaggcg ctgatcgcgc gcggcgtgat cggcgactac cgcgagccgt acgtgctgcg cttcggcttc acgccgctct atacgcgctt cgccgacgtg tgggatgcgg tcgagacgct gcgcgacatc ctcgccaccg acgcgtggaa ggcgcccgag ttcgccgaac gcggcgcggt gacctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for BuceA.00009.a.B1.PS01723

PURIFICATION DATe:	1/15/2013
CONCENTRATION:	71mg/ml
OBSERVED MW:	44kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	5
VIAL VOLUME:	110µl
PERCENT IDENTITY:	79
PERCENT COVERAGE:	90

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMI KTREDALALD RDDPLAPLRD QFSLPDGVIY LDGNSLGAQP RASAARAQQV IGAEWGEGLI RSWNTAGWFA LPRRLGDKLA TLIGGAPGET VVTDTISINL FKLLSAMLRH QAERAPERRV IVSERSNFPT DLYIAQGLID QLGGGYTLRL IDDPADLPGA LGADTAVAMI THVNYRTGYM HDMPAVTQLV HDAGALMLWD LAHSAGAVPV DLNGARADGA VGCTYKYLNG GPGSPAFVWV PQRHLAHFSQ PLSGWWGHRA PFAMQPGFAP DPGIARFLCG TQPIVSMSMV ECGLDVFLQT DMDAIRRKSL ALTDAFIALV EARCAGLSLK LVTPRAHHQR GSQASFEHPH GYEVMQALIA RGVIGDYREP YVLRFGFTPL YTRFADVWDA VETLRDILAT DAWKAPEFAE RGAVT

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGATCA AGACCCGTGA AGACGCGCTC GCGCTCGACC GCGACGACCC GCTGGCCCCG CTGCGCGACC AGTTCTCGCT GCCGGACGGC GTGATCTACC TCGACGGCAA CTCGCTCGGC GCGCAGCCAC GTGCATCGGC CGCCCGCGCG CAGCAGGTGA TCGGCGCCGA ATGGGGCGAA GGCCTGATCC GCAGCTGGAA CACGGCCGGC TGGTTCGCGC TGCCGCGCCG CCTCGGCGAC AAGCTCGCGA CGCTGATCGG CGGCGCGCCC GGCGAGACGG TCGTCACCGA CACCATCTCG ATCAACCTGT TCAAGCTGCT GTCGGCGATG CTGCGCCACC AGGCCGAGCG TGCGCCCGAG CGCCGGGTGA TCGTGTCGGA GCGCTCGAAC TTCCCGACCG ACCTGTACAT CGCGCAGGGG CTGATCGACC AGCTCGGCGG CGGCTACACG CTGCGCCTGA TCGACGATCC GGCCGACCTG CCCGGCGCGC TCGGCGCGGA CACGGCCGTC GCGATGATCA CGCACGTGAA CTACCGCACC GGCTACATGC ACGACATGCC GGCCGTCACA CAGCTGGTGC ACGACGCGGG CGCGCTGATG CTGTGGGATC TCGCGCACTC GGCCGGCGCA GTGCCGGTCG ACCTGAACGG CGCGCGCGCG GACGGTGCGG TCGGCTGCAC GTACAAGTAC CTGAACGGCG GCCCCGGCTC GCCCGCGTTC GTGTGGGTGC CGCAACGCCA TCTCGCGCAT TTCTCGCAGC CGCTGTCCGG CTGGTGGGGC CACCGCGCGC CGTTCGCGAT GCAGCCGGGC TTCGCGCCCG ATCCGGGCAT CGCGCGCTTC CTGTGCGGCA CGCAGCCGAT CGTGTCGATG TCGATGGTCG AATGCGGGCT CGACGTGTTC CTGCAGACCG ACATGGACGC GATCCGCCGC AAGTCGCTCG CGTTGACCGA TGCCTTCATC GCGCTCGTCG AAGCGCGCTG CGCGGGCCTG TCGCTGAAGC TCGTCACGCC GCGTGCGCAT CACCAGCGCG GCTCGCAGGC GAGCTTCGAG CATCCGCACG GCTACGAAGT GATGCAGGCG CTGATCGCGC GCGGCGTGAT CGGCGACTAC CGCGAGCCGT ACGTGCTGCG CTTCGGCTTC ACGCCGCTCT ATACGCGCTT CGCCGACGTG TGGGATGCGG TCGAGACGCT GCGCGACATC CTCGCCACCG ACGCGTGGAA GGCGCCCGAG TTCGCCGAAC GCGGCGCGGT GACCTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT