BuceA.00008.a
Serine hydroxymethyltransferase
| CENTER ID: | BuceA.00008.a |
| ORGANISM: | Burkholderia cenocepacia J2315 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.00008.a.B1.GE36327 | Full length( BuceA.00008.a ) | 1 | 415 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.00008.a.B1.PW37267 | Full length( BuceA.00008.a ) | 1 | 415 |
Structures
4MSO
DEPOSITED: 9/18/2013
DETERMINATION: XRay
CLONE: BuceA.00008.a.B1.GE36327
PROTEIN: BuceA.00008.a.B1.PW37267
4N0W
DEPOSITED: 10/2/2013
DETERMINATION: XRay
CLONE: BuceA.00008.a.B1.GE36327
PROTEIN: BuceA.00008.a.B1.PW37267
4OTL
DEPOSITED: 2/13/2014
DETERMINATION: XRay
CLONE: BuceA.00008.a.B1.GE36327
PROTEIN: BuceA.00008.a.B1.PW37267
4OT8
DEPOSITED: 2/13/2014
DETERMINATION: XRay
CLONE: BuceA.00008.a.B1.GE36327
PROTEIN: BuceA.00008.a.B1.PW37267
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|216591.5.peg.3320 |
| RefSeq: | YP_002232306.1 |
| UniProt: | B4ECY9 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFDRAQSTIA NVDPEIFAAI EQENRRQEDH IELIASENYT SPAVMAAQGS QLTNKYAEGY PGKRYYGGCE YVDVVEQLAI DRVKQLFGAE AANVQPNSGS QANQGVFFAM LKPGDTIMGM SLAHGGHLTH GSPVNMSGKW FNVVSYGLNE NEDIDYDAAE KLANEHKPKL IVAGASAFAL KIDFERLAKI AKSVGAYLMV DMAHYAGLIA AGVYPNPVPH ADFVTTTTHK SLRGPRGGVI LMKAEYEKPI NSAIFPGIQG GPLMHVIAAK AVAFKEALSP EFKEYQQKVV ENARVLAETL VKRGLRIVSG RTESHVMLVD LRAKHITGKA AEAALGAAHI TVNKNAIPND PEKPFVTSGI RLGSPAMTTR GFGPAEAEQV GNLIADVLEN PEDAATIERV RAQVAELTKR FPVYR
NT Sequence
ATGTTTGACA GAGCCCAAAG CACCATCGCG AACGTCGATC CCGAAATCTT TGCAGCGATC GAGCAGGAAA ACCGCCGCCA GGAAGATCAC ATCGAGCTGA TCGCGTCGGA AAACTACACG AGCCCGGCCG TGATGGCCGC GCAGGGCTCG CAGCTCACGA ACAAGTACGC GGAAGGGTAT CCGGGCAAGC GCTACTACGG CGGTTGCGAG TACGTGGACG TGGTCGAGCA GCTGGCGATC GACCGCGTGA AGCAGCTGTT CGGCGCGGAA GCGGCGAACG TGCAGCCGAA CTCGGGTTCG CAGGCGAACC AGGGCGTGTT CTTCGCGATG CTCAAGCCGG GTGACACGAT CATGGGCATG AGCCTCGCGC ACGGCGGCCA CCTGACGCAC GGCTCGCCGG TGAACATGTC GGGCAAGTGG TTCAACGTGG TGAGCTACGG CCTGAACGAG AACGAAGACA TCGACTACGA CGCGGCCGAG AAGCTGGCGA ACGAACACAA GCCGAAGCTG ATCGTCGCGG GCGCATCGGC ATTCGCGCTG AAGATCGATT TCGAGCGTCT GGCGAAGATC GCGAAGTCGG TCGGCGCATA CCTGATGGTC GACATGGCGC ACTACGCAGG CCTGATCGCG GCGGGCGTGT ACCCGAACCC GGTGCCGCAC GCGGATTTCG TGACGACGAC GACGCACAAG AGCCTGCGCG GCCCGCGCGG CGGCGTGATC CTGATGAAGG CGGAATACGA GAAGCCGATC AACTCGGCGA TCTTCCCGGG GATCCAGGGC GGCCCGCTGA TGCACGTGAT CGCGGCGAAG GCGGTGGCGT TCAAGGAAGC GCTGTCGCCG GAGTTCAAGG AATATCAGCA GAAGGTGGTC GAGAACGCAC GCGTGCTGGC CGAAACGCTG GTCAAGCGCG GGCTGCGGAT CGTGTCGGGC CGTACGGAAA GCCACGTGAT GCTGGTGGAC CTGCGCGCGA AGCACATCAC GGGCAAGGCG GCGGAAGCGG CGCTCGGCGC GGCGCACATC ACGGTGAACA AGAACGCGAT TCCGAACGAC CCGGAAAAGC CGTTCGTGAC GAGCGGCATC CGTCTCGGCT CGCCGGCGAT GACGACGCGC GGCTTCGGGC CGGCGGAAGC CGAGCAGGTC GGCAACCTGA TCGCCGACGT GCTGGAGAAC CCGGAAGACG CAGCGACGAT CGAGCGCGTG CGCGCGCAGG TCGCCGAGCT GACCAAGCGT TTCCCGGTCT ATCGC
Details for BuceA.00008.a.B1.GE36327
| HARVESTED ON: | 10/23/2012 |
| SEQUENCED ON: | 10/26/2012 |
| EXPECTED MW: | 45kDa |
| OBSERVED MW: | 45kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 85 |
Validated AA Sequence
MAAQGSQLTN KYAEGYPGKR YYGGCEYVDV VEQLAIDRVK QLFGAEAANV QPNSGSQANQ GVFFAMLKPG DTIMGMSLAH GGHLTHGSPV NMSGKWFNVV SYGLNENEDI DYDAAEKLAN EHKPKLIVAG ASAFALKIDF ERLAKIAKSV GAYLMVDMAH YAGLIAAGVY PNPVPHADFV TTTTHKSLRG PRGGVILMKA EYEKPINSAI FPGIQGGPLM HVIAAKAVAF KEALSPEFKE YQQKVVENAR VLAETLVKRG LRIVSGRTES HVMLVDLRAK HITGKAAEAA LGAAHITVNK NAIPNDPEKP FVTSGIRLGS PAMTTRGFGP AEAEQVGNLI ADVLENPEDA ATIERVRAQV AE
Validated NT Sequence
cagctcggcg acctgcgcgc gcacgcgctc gatcgtcgct gcgtcttccg ggttctccag cacgtcggcg atcaggttgc cgacctgctc ggcttccgcc ggcccgaagc cgcgcgtcgt catcgccggc gagccgagac ggatgccgct cgtcacgaac ggcttttccg ggtcgttcgg aatcgcgttc ttgttcaccg tgatgtgcgc cgcgccgagc gccgcttccg ccgccttgcc cgtgatgtgc ttcgcgcgca ggtccaccag catcacgtgg ctttccgtac ggcccgacac gatccgcagc ccgcgcttga ccagcgtttc ggccagcacg cgtgcgttct cgaccacctt ctgctgatat tccttgaact ccggcgacag cgcttccttg aacgccaccg ccttcgccgc gatcacgtgc atcagcgggc cgccctggat ccccgggaag atcgccgagt tgatcggctt ctcgtattcc gccttcatca ggatcacgcc gccgcgcggg ccgcgcaggc tcttgtgcgt cgtcgtcgtc acgaaatccg cgtgcggcac cgggttcggg tacacgcccg ccgcgatcag gcctgcgtag tgcgccatgt cgaccatcag gtatgcgccg accgacttcg cgatcttcgc cagacgctcg aaatcgatct tcagcgcgaa tgccgatgcg cccgcgacga tcagcttcgg cttgtgttcg ttcgccagct tctcggccgc gtcgtagtcg atgtcttcgt tctcgttcag gccgtagctc accacgttga accacttgcc cgacatgttc accggcgagc cgtgcgtcag gtggccgccg tgcgcgaggc tcatgcccat gatcgtgtca cccggcttga gcatcgcgaa gaacacgccc tggttcgcct gcgaacccga gttcggctgc acgttcgccg cttccgcgcc gaacagctgc ttcacgcggt cgatcgccag ctgctcgacc acgtccacgt actcgcaacc gccgtagtag cgcttgcccg gatacccttc cgcgtacttg ttcgtgagct gcgagccctg cgcggccatc acggccgggc tcgtgtagtt ttccgacgcg atcagctcga tgtgatcttc ctggcggcgg ttttcctgct cgatcgctgc aaagatttcg ggatcgacgt tcgcgatggt gctttgggct ctgtc
Expected Protein Sequence
MAHHHHHHMF DRAQSTIANV DPEIFAAIEQ ENRRQEDHIE LIASENYTSP AVMAAQGSQL TNKYAEGYPG KRYYGGCEYV DVVEQLAIDR VKQLFGAEAA NVQPNSGSQA NQGVFFAMLK PGDTIMGMSL AHGGHLTHGS PVNMSGKWFN VVSYGLNENE DIDYDAAEKL ANEHKPKLIV AGASAFALKI DFERLAKIAK SVGAYLMVDM AHYAGLIAAG VYPNPVPHAD FVTTTTHKSL RGPRGGVILM KAEYEKPINS AIFPGIQGGP LMHVIAAKAV AFKEALSPEF KEYQQKVVEN ARVLAETLVK RGLRIVSGRT ESHVMLVDLR AKHITGKAAE AALGAAHITV NKNAIPNDPE KPFVTSGIRL GSPAMTTRGF GPAEAEQVGN LIADVLENPE DAATIERVRA QVAELTKRFP VYR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtttg acagagccca aagcaccatc gcgaacgtcg atcccgaaat ctttgcagcg atcgagcagg aaaaccgccg ccaggaagat cacatcgagc tgatcgcgtc ggaaaactac acgagcccgg ccgtgatggc cgcgcagggc tcgcagctca cgaacaagta cgcggaaggg tatccgggca agcgctacta cggcggttgc gagtacgtgg acgtggtcga gcagctggcg atcgaccgcg tgaagcagct gttcggcgcg gaagcggcga acgtgcagcc gaactcgggt tcgcaggcga accagggcgt gttcttcgcg atgctcaagc cgggtgacac gatcatgggc atgagcctcg cgcacggcgg ccacctgacg cacggctcgc cggtgaacat gtcgggcaag tggttcaacg tggtgagcta cggcctgaac gagaacgaag acatcgacta cgacgcggcc gagaagctgg cgaacgaaca caagccgaag ctgatcgtcg cgggcgcatc ggcattcgcg ctgaagatcg atttcgagcg tctggcgaag atcgcgaagt cggtcggcgc atacctgatg gtcgacatgg cgcactacgc aggcctgatc gcggcgggcg tgtacccgaa cccggtgccg cacgcggatt tcgtgacgac gacgacgcac aagagcctgc gcggcccgcg cggcggcgtg atcctgatga aggcggaata cgagaagccg atcaactcgg cgatcttccc ggggatccag ggcggcccgc tgatgcacgt gatcgcggcg aaggcggtgg cgttcaagga agcgctgtcg ccggagttca aggaatatca gcagaaggtg gtcgagaacg cacgcgtgct ggccgaaacg ctggtcaagc gcgggctgcg gatcgtgtcg ggccgtacgg aaagccacgt gatgctggtg gacctgcgcg cgaagcacat cacgggcaag gcggcggaag cggcgctcgg cgcggcgcac atcacggtga acaagaacgc gattccgaac gacccggaaa agccgttcgt gacgagcggc atccgtctcg gctcgccggc gatgacgacg cgcggcttcg ggccggcgga agccgagcag gtcggcaacc tgatcgccga cgtgctggag aacccggaag acgcagcgac gatcgagcgc gtgcgcgcgc aggtcgccga gctgaccaag cgtttcccgg tctatcgctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for BuceA.00008.a.B1.PW37267
| PURIFICATION DATe: | 3/25/2013 |
| CONCENTRATION: | 34.4mg/ml |
| OBSERVED MW: | 48kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 18 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 85 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAAQGSQLTN KYAEGYPGKR YYGGCEYVDV VEQLAIDRVK QLFGAEAANV QPNSGSQANQ GVFFAMLKPG DTIMGMSLAH GGHLTHGSPV NMSGKWFNVV SYGLNENEDI DYDAAEKLAN EHKPKLIVAG ASAFALKIDF ERLAKIAKSV GAYLMVDMAH YAGLIAAGVY PNPVPHADFV TTTTHKSLRG PRGGVILMKA EYEKPINSAI FPGIQGGPLM HVIAAKAVAF KEALSPEFKE YQQKVVENAR VLAETLVKRG LRIVSGRTES HVMLVDLRAK HITGKAAEAA LGAAHITVNK NAIPNDPEKP FVTSGIRLGS PAMTTRGFGP AEAEQVGNLI ADVLENPEDA ATIERVRAQV AE
Validated NT Sequence
cagctcggcg acctgcgcgc gcacgcgctc gatcgtcgct gcgtcttccg ggttctccag cacgtcggcg atcaggttgc cgacctgctc ggcttccgcc ggcccgaagc cgcgcgtcgt catcgccggc gagccgagac ggatgccgct cgtcacgaac ggcttttccg ggtcgttcgg aatcgcgttc ttgttcaccg tgatgtgcgc cgcgccgagc gccgcttccg ccgccttgcc cgtgatgtgc ttcgcgcgca ggtccaccag catcacgtgg ctttccgtac ggcccgacac gatccgcagc ccgcgcttga ccagcgtttc ggccagcacg cgtgcgttct cgaccacctt ctgctgatat tccttgaact ccggcgacag cgcttccttg aacgccaccg ccttcgccgc gatcacgtgc atcagcgggc cgccctggat ccccgggaag atcgccgagt tgatcggctt ctcgtattcc gccttcatca ggatcacgcc gccgcgcggg ccgcgcaggc tcttgtgcgt cgtcgtcgtc acgaaatccg cgtgcggcac cgggttcggg tacacgcccg ccgcgatcag gcctgcgtag tgcgccatgt cgaccatcag gtatgcgccg accgacttcg cgatcttcgc cagacgctcg aaatcgatct tcagcgcgaa tgccgatgcg cccgcgacga tcagcttcgg cttgtgttcg ttcgccagct tctcggccgc gtcgtagtcg atgtcttcgt tctcgttcag gccgtagctc accacgttga accacttgcc cgacatgttc accggcgagc cgtgcgtcag gtggccgccg tgcgcgaggc tcatgcccat gatcgtgtca cccggcttga gcatcgcgaa gaacacgccc tggttcgcct gcgaacccga gttcggctgc acgttcgccg cttccgcgcc gaacagctgc ttcacgcggt cgatcgccag ctgctcgacc acgtccacgt actcgcaacc gccgtagtag cgcttgcccg gatacccttc cgcgtacttg ttcgtgagct gcgagccctg cgcggccatc acggccgggc tcgtgtagtt ttccgacgcg atcagctcga tgtgatcttc ctggcggcgg ttttcctgct cgatcgctgc aaagatttcg ggatcgacgt tcgcgatggt gctttgggct ctgtc
Expressed Protein Sequence
MAHHHHHHMF DRAQSTIANV DPEIFAAIEQ ENRRQEDHIE LIASENYTSP AVMAAQGSQL TNKYAEGYPG KRYYGGCEYV DVVEQLAIDR VKQLFGAEAA NVQPNSGSQA NQGVFFAMLK PGDTIMGMSL AHGGHLTHGS PVNMSGKWFN VVSYGLNENE DIDYDAAEKL ANEHKPKLIV AGASAFALKI DFERLAKIAK SVGAYLMVDM AHYAGLIAAG VYPNPVPHAD FVTTTTHKSL RGPRGGVILM KAEYEKPINS AIFPGIQGGP LMHVIAAKAV AFKEALSPEF KEYQQKVVEN ARVLAETLVK RGLRIVSGRT ESHVMLVDLR AKHITGKAAE AALGAAHITV NKNAIPNDPE KPFVTSGIRL GSPAMTTRGF GPAEAEQVGN LIADVLENPE DAATIERVRA QVAELTKRFP VYR
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTTTG ACAGAGCCCA AAGCACCATC GCGAACGTCG ATCCCGAAAT CTTTGCAGCG ATCGAGCAGG AAAACCGCCG CCAGGAAGAT CACATCGAGC TGATCGCGTC GGAAAACTAC ACGAGCCCGG CCGTGATGGC CGCGCAGGGC TCGCAGCTCA CGAACAAGTA CGCGGAAGGG TATCCGGGCA AGCGCTACTA CGGCGGTTGC GAGTACGTGG ACGTGGTCGA GCAGCTGGCG ATCGACCGCG TGAAGCAGCT GTTCGGCGCG GAAGCGGCGA ACGTGCAGCC GAACTCGGGT TCGCAGGCGA ACCAGGGCGT GTTCTTCGCG ATGCTCAAGC CGGGTGACAC GATCATGGGC ATGAGCCTCG CGCACGGCGG CCACCTGACG CACGGCTCGC CGGTGAACAT GTCGGGCAAG TGGTTCAACG TGGTGAGCTA CGGCCTGAAC GAGAACGAAG ACATCGACTA CGACGCGGCC GAGAAGCTGG CGAACGAACA CAAGCCGAAG CTGATCGTCG CGGGCGCATC GGCATTCGCG CTGAAGATCG ATTTCGAGCG TCTGGCGAAG ATCGCGAAGT CGGTCGGCGC ATACCTGATG GTCGACATGG CGCACTACGC AGGCCTGATC GCGGCGGGCG TGTACCCGAA CCCGGTGCCG CACGCGGATT TCGTGACGAC GACGACGCAC AAGAGCCTGC GCGGCCCGCG CGGCGGCGTG ATCCTGATGA AGGCGGAATA CGAGAAGCCG ATCAACTCGG CGATCTTCCC GGGGATCCAG GGCGGCCCGC TGATGCACGT GATCGCGGCG AAGGCGGTGG CGTTCAAGGA AGCGCTGTCG CCGGAGTTCA AGGAATATCA GCAGAAGGTG GTCGAGAACG CACGCGTGCT GGCCGAAACG CTGGTCAAGC GCGGGCTGCG GATCGTGTCG GGCCGTACGG AAAGCCACGT GATGCTGGTG GACCTGCGCG CGAAGCACAT CACGGGCAAG GCGGCGGAAG CGGCGCTCGG CGCGGCGCAC ATCACGGTGA ACAAGAACGC GATTCCGAAC GACCCGGAAA AGCCGTTCGT GACGAGCGGC ATCCGTCTCG GCTCGCCGGC GATGACGACG CGCGGCTTCG GGCCGGCGGA AGCCGAGCAG GTCGGCAACC TGATCGCCGA CGTGCTGGAG AACCCGGAAG ACGCAGCGAC GATCGAGCGC GTGCGCGCGC AGGTCGCCGA GCTGACCAAG CGTTTCCCGG TCTATCGCTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT