BuamA.17267.a

SurA domain protein

CENTER ID: BuamA.17267.a
ORGANISM: Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.17267.a.A2.GE31820 Mature protein( BuamA.17267.a ) 30 452
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.17267.a.A2.PW31727 Mature protein( BuamA.17267.a ) 30 452

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|398577.6.peg.2706
RefSeq: YP_001809321.1
UniProt: B1YWD7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKKTLRFAAV VSSLAASAAL LAAAPAAAQA LGSRGAQLAD EVVAVVNNDV ITGRELDQRV GLIARRLQQQ NAPVPPADQL RAQVLNQMVL ERIQVQKAKD DGIRIDDATV QSTLQRLAQA NGMTLEQYRA RLEAQGVPWS VFTSDARTEL MLSKLREREV DGKITVSDAE VVNYIASQRG PSASQQQDLR FQHIFIKAPT NAPQAEIEVA QKKAEALLQQ ATSGADFEKL AKNNSEANDA KKGGDLGFKA PGALPAEVVD AASKLRPGQV NPTLIRVPDG FEIVRLVDRR QSQGTSAAAP KIVQTHVRHI LLRVGEGKSE GQARQQLQDI RRQVEAGGDF AKFARTYSQD GSASQGGDLG WISPGETVPE FERAMNNLQD GQISQPVRTE YGYHLIQVID RRESEGSVQQ QMDIARQAIG QRKAEQAYAD WLRELRDSSY VQYKIGGVGP AN
NT Sequence
ATGAAGAAAA CCCTTCGTTT CGCGGCAGTC GTGTCCAGCC TCGCCGCGTC CGCCGCGCTG CTCGCCGCCG CGCCGGCCGC GGCGCAGGCG CTTGGTTCGC GAGGCGCGCA GCTCGCCGAC GAAGTCGTCG CGGTCGTCAA CAACGACGTG ATCACGGGCC GCGAACTCGA CCAGCGCGTC GGCCTGATCG CGCGCCGGCT GCAGCAGCAG AACGCACCGG TGCCGCCGGC CGACCAGTTG CGTGCGCAGG TGCTGAACCA GATGGTCCTC GAACGCATCC AGGTGCAGAA GGCCAAGGAC GACGGGATCC GCATCGACGA TGCGACCGTG CAGTCCACGC TGCAGCGCCT CGCGCAGGCG AACGGGATGA CGCTCGAACA GTATCGCGCG CGTCTCGAGG CGCAAGGCGT GCCCTGGAGC GTGTTCACGA GCGACGCCCG CACCGAGCTG ATGCTGTCGA AGCTGCGCGA GCGCGAGGTC GACGGCAAGA TCACCGTGTC GGACGCCGAG GTCGTGAACT ACATCGCGAG CCAGCGCGGG CCGAGCGCGT CGCAGCAGCA GGACCTGCGC TTCCAGCACA TCTTCATCAA GGCGCCGACC AACGCGCCGC AGGCCGAGAT CGAAGTCGCG CAGAAGAAGG CCGAAGCGCT GCTCCAGCAG GCCACGTCGG GCGCCGATTT CGAGAAGCTC GCGAAGAACA ATTCGGAAGC GAACGACGCG AAGAAGGGCG GCGACCTCGG CTTCAAGGCG CCGGGCGCGC TGCCGGCGGA GGTTGTCGAC GCCGCATCGA AGCTGCGCCC GGGCCAGGTC AACCCGACGC TGATCCGCGT GCCGGACGGT TTCGAGATCG TGCGTCTCGT CGACCGCCGC CAGAGCCAGG GCACGAGCGC CGCGGCACCG AAGATCGTCC AGACGCACGT GCGCCACATC CTGTTGCGCG TCGGCGAGGG CAAGTCGGAA GGCCAGGCCC GTCAGCAGCT GCAGGACATT CGCCGGCAGG TCGAGGCGGG CGGCGATTTC GCGAAGTTCG CGCGCACCTA CTCGCAGGAC GGTTCGGCGT CGCAGGGCGG CGATCTCGGC TGGATCAGCC CGGGCGAGAC CGTGCCGGAA TTCGAGCGCG CGATGAACAA CCTGCAGGAC GGCCAGATCA GCCAGCCGGT GCGTACCGAG TACGGTTACC ACCTGATCCA GGTGATCGAT CGCCGCGAGT CGGAAGGCTC GGTGCAGCAG CAGATGGATA TCGCGCGTCA GGCGATCGGC CAGCGCAAGG CCGAGCAGGC GTATGCCGAC TGGCTGCGCG AACTGCGCGA TTCGTCCTAC GTGCAGTACA AGATCGGCGG CGTCGGCCCG GCGAAC
Details for BuamA.17267.a.A2.GE31820
HARVESTED ON: 3/8/2011
SEQUENCED ON: 3/14/2011
EXPECTED MW: 48kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 91
PERCENT COVERAGE: 72
Validated AA Sequence
MTLEQYRARL EAXGXPWSVX TSXXXXXXML SXLRERXXXG XITXXXAEVV NYIXXXRGXS AXXQQDLRXX XIFIKAPTNA PQAEIEVAQK KAEALLQQAT SGADFEKLAK NNSEANDAKK GGDLGFKAPG ALPAEVVDAA SKLRPGQVNP TLIRVPDGFE IVRLVDRRQS QGTSAAAPKI VQTHVRHILL RVGEGKSEGQ ARQQLQDIRR QVEAGGDFAK FARTYSQDGS ASQGGDLGWI SPGETVPEFE RAMNNLQDGQ ISQPVRTEYG YHLIQVIDRR ESEGSVQQQM DIARQAIGQR KAEQAYADWL RELRDSSYVQ YKI
Validated NT Sequence
cgatcttgta ctgcacgtag gacgaatcgc gcagttcgcg cagccagtcg gcatacgcct gctcggcctt gcgctggccg atcgcctgac gcgcgatatc catctgctgc tgcaccgagc cttccgactc gcggcgatcg atcacctgga tcaggtggta accgtactcg gtacgcaccg gctggctgat ctggccgtcc tgcaggttgt tcatcgcgcg ctcgaattcc ggcacggtct cgcccgggct gatccagccg agatcgccgc cctgcgacgc cgaaccgtcc tgcgagtagg tgcgcgcgaa cttcgcgaaa tcgccgcccg cctcgacctg ccggcgaatg tcctgcagct gctgacgggc ctggccttcc gacttgccct cgccgacgcg caacaggatg tggcgcacgt gcgtctggac gatcttcggt gccgcggcgc tcgtgccctg gctctggcgg cggtcgacga gacgcacgat ctcgaaaccg tccggcacgc ggatcagcgt cgggttgacc tggcccgggc gcagcttcga tgcggcgtcg acaacctccg ccggcagcgc gcccggcgcc ttgaagccga ggtcgccgcc cttcttcgcg tcgttcgctt ccgaattgtt cttcgcgagc ttctcgaaat cggcgcccga cgtggcctgc tggagcagcg cttcggcctt cttctgcgcg acttcgatct cggcctgcgg cgcgttggtc ggcgccttga tgaagatgng cnnnnancgc aggtcctgct gcnnnnacgc gctcgncccg cgctnnctnn ngatgtagtt cacgacctcg gcntnnnncn cngtgatctn gccgtnnncn tcgcgctcgc gcagnttcga cagcatcanc tnnnnncnga nnncgctcgt gnacacgctc cagggcnngc cntgcgcctc gagacgcgcg cgatactgtt cgagcgtcat ccngttngcn tgcgcgaggc gntgcagng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMALGSRGAQ LADEVVAVVN NDVITGRELD QRVGLIARRL QQQNAPVPPA DQLRAQVLNQ MVLERIQVQK AKDDGIRIDD ATVQSTLQRL AQANGMTLEQ YRARLEAQGV PWSVFTSDAR TELMLSKLRE REVDGKITVS DAEVVNYIAS QRGPSASQQQ DLRFQHIFIK APTNAPQAEI EVAQKKAEAL LQQATSGADF EKLAKNNSEA NDAKKGGDLG FKAPGALPAE VVDAASKLRP GQVNPTLIRV PDGFEIVRLV DRRQSQGTSA AAPKIVQTHV RHILLRVGEG KSEGQARQQL QDIRRQVEAG GDFAKFARTY SQDGSASQGG DLGWISPGET VPEFERAMNN LQDGQISQPV RTEYGYHLIQ VIDRRESEGS VQQQMDIARQ AIGQRKAEQA YADWLRELRD SSYVQYKIGG VGPAN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgcttggt tcgcgaggcg cgcagctcgc cgacgaagtc gtcgcggtcg tcaacaacga cgtgatcacg ggccgcgaac tcgaccagcg cgtcggcctg atcgcgcgcc ggctgcagca gcagaacgca ccggtgccgc cggccgacca gttgcgtgcg caggtgctga accagatggt cctcgaacgc atccaggtgc agaaggccaa ggacgacggg atccgcatcg acgatgcgac cgtgcagtcc acgctgcagc gcctcgcgca ggcgaacggg atgacgctcg aacagtatcg cgcgcgtctc gaggcgcaag gcgtgccctg gagcgtgttc acgagcgacg cccgcaccga gctgatgctg tcgaagctgc gcgagcgcga ggtcgacggc aagatcaccg tgtcggacgc cgaggtcgtg aactacatcg cgagccagcg cgggccgagc gcgtcgcagc agcaggacct gcgcttccag cacatcttca tcaaggcgcc gaccaacgcg ccgcaggccg agatcgaagt cgcgcagaag aaggccgaag cgctgctcca gcaggccacg tcgggcgccg atttcgagaa gctcgcgaag aacaattcgg aagcgaacga cgcgaagaag ggcggcgacc tcggcttcaa ggcgccgggc gcgctgccgg cggaggttgt cgacgccgca tcgaagctgc gcccgggcca ggtcaacccg acgctgatcc gcgtgccgga cggtttcgag atcgtgcgtc tcgtcgaccg ccgccagagc cagggcacga gcgccgcggc accgaagatc gtccagacgc acgtgcgcca catcctgttg cgcgtcggcg agggcaagtc ggaaggccag gcccgtcagc agctgcagga cattcgccgg caggtcgagg cgggcggcga tttcgcgaag ttcgcgcgca cctactcgca ggacggttcg gcgtcgcagg gcggcgatct cggctggatc agcccgggcg agaccgtgcc ggaattcgag cgcgcgatga acaacctgca ggacggccag atcagccagc cggtgcgtac cgagtacggt taccacctga tccaggtgat cgatcgccgc gagtcggaag gctcggtgca gcagcagatg gatatcgcgc gtcaggcgat cggccagcgc aaggccgagc aggcgtatgc cgactggctg cgcgaactgc gcgattcgtc ctacgtgcag tacaagatcg gcggcgtcgg cccggcgaac taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for BuamA.17267.a.A2.PW31727
PURIFICATION DATe: 5/13/2011
CONCENTRATION: 29.81mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: 91
PERCENT COVERAGE: 72
Protocol Notes
notes unavailable
Validated AA Sequence
MTLEQYRARL EAXGXPWSVX TSXXXXXXML SXLRERXXXG XITXXXAEVV NYIXXXRGXS AXXQQDLRXX XIFIKAPTNA PQAEIEVAQK KAEALLQQAT SGADFEKLAK NNSEANDAKK GGDLGFKAPG ALPAEVVDAA SKLRPGQVNP TLIRVPDGFE IVRLVDRRQS QGTSAAAPKI VQTHVRHILL RVGEGKSEGQ ARQQLQDIRR QVEAGGDFAK FARTYSQDGS ASQGGDLGWI SPGETVPEFE RAMNNLQDGQ ISQPVRTEYG YHLIQVIDRR ESEGSVQQQM DIARQAIGQR KAEQAYADWL RELRDSSYVQ YKI
Validated NT Sequence
cgatcttgta ctgcacgtag gacgaatcgc gcagttcgcg cagccagtcg gcatacgcct gctcggcctt gcgctggccg atcgcctgac gcgcgatatc catctgctgc tgcaccgagc cttccgactc gcggcgatcg atcacctgga tcaggtggta accgtactcg gtacgcaccg gctggctgat ctggccgtcc tgcaggttgt tcatcgcgcg ctcgaattcc ggcacggtct cgcccgggct gatccagccg agatcgccgc cctgcgacgc cgaaccgtcc tgcgagtagg tgcgcgcgaa cttcgcgaaa tcgccgcccg cctcgacctg ccggcgaatg tcctgcagct gctgacgggc ctggccttcc gacttgccct cgccgacgcg caacaggatg tggcgcacgt gcgtctggac gatcttcggt gccgcggcgc tcgtgccctg gctctggcgg cggtcgacga gacgcacgat ctcgaaaccg tccggcacgc ggatcagcgt cgggttgacc tggcccgggc gcagcttcga tgcggcgtcg acaacctccg ccggcagcgc gcccggcgcc ttgaagccga ggtcgccgcc cttcttcgcg tcgttcgctt ccgaattgtt cttcgcgagc ttctcgaaat cggcgcccga cgtggcctgc tggagcagcg cttcggcctt cttctgcgcg acttcgatct cggcctgcgg cgcgttggtc ggcgccttga tgaagatgng cnnnnancgc aggtcctgct gcnnnnacgc gctcgncccg cgctnnctnn ngatgtagtt cacgacctcg gcntnnnncn cngtgatctn gccgtnnncn tcgcgctcgc gcagnttcga cagcatcanc tnnnnncnga nnncgctcgt gnacacgctc cagggcnngc cntgcgcctc gagacgcgcg cgatactgtt cgagcgtcat ccngttngcn tgcgcgaggc gntgcagng
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMALGSRGAQ LADEVVAVVN NDVITGRELD QRVGLIARRL QQQNAPVPPA DQLRAQVLNQ MVLERIQVQK AKDDGIRIDD ATVQSTLQRL AQANGMTLEQ YRARLEAQGV PWSVFTSDAR TELMLSKLRE REVDGKITVS DAEVVNYIAS QRGPSASQQQ DLRFQHIFIK APTNAPQAEI EVAQKKAEAL LQQATSGADF EKLAKNNSEA NDAKKGGDLG FKAPGALPAE VVDAASKLRP GQVNPTLIRV PDGFEIVRLV DRRQSQGTSA AAPKIVQTHV RHILLRVGEG KSEGQARQQL QDIRRQVEAG GDFAKFARTY SQDGSASQGG DLGWISPGET VPEFERAMNN LQDGQISQPV RTEYGYHLIQ VIDRRESEGS VQQQMDIARQ AIGQRKAEQA YADWLRELRD SSYVQYKIGG VGPAN
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGCTTGGT TCGCGAGGCG CGCAGCTCGC CGACGAAGTC GTCGCGGTCG TCAACAACGA CGTGATCACG GGCCGCGAAC TCGACCAGCG CGTCGGCCTG ATCGCGCGCC GGCTGCAGCA GCAGAACGCA CCGGTGCCGC CGGCCGACCA GTTGCGTGCG CAGGTGCTGA ACCAGATGGT CCTCGAACGC ATCCAGGTGC AGAAGGCCAA GGACGACGGG ATCCGCATCG ACGATGCGAC CGTGCAGTCC ACGCTGCAGC GCCTCGCGCA GGCGAACGGG ATGACGCTCG AACAGTATCG CGCGCGTCTC GAGGCGCAAG GCGTGCCCTG GAGCGTGTTC ACGAGCGACG CCCGCACCGA GCTGATGCTG TCGAAGCTGC GCGAGCGCGA GGTCGACGGC AAGATCACCG TGTCGGACGC CGAGGTCGTG AACTACATCG CGAGCCAGCG CGGGCCGAGC GCGTCGCAGC AGCAGGACCT GCGCTTCCAG CACATCTTCA TCAAGGCGCC GACCAACGCG CCGCAGGCCG AGATCGAAGT CGCGCAGAAG AAGGCCGAAG CGCTGCTCCA GCAGGCCACG TCGGGCGCCG ATTTCGAGAA GCTCGCGAAG AACAATTCGG AAGCGAACGA CGCGAAGAAG GGCGGCGACC TCGGCTTCAA GGCGCCGGGC GCGCTGCCGG CGGAGGTTGT CGACGCCGCA TCGAAGCTGC GCCCGGGCCA GGTCAACCCG ACGCTGATCC GCGTGCCGGA CGGTTTCGAG ATCGTGCGTC TCGTCGACCG CCGCCAGAGC CAGGGCACGA GCGCCGCGGC ACCGAAGATC GTCCAGACGC ACGTGCGCCA CATCCTGTTG CGCGTCGGCG AGGGCAAGTC GGAAGGCCAG GCCCGTCAGC AGCTGCAGGA CATTCGCCGG CAGGTCGAGG CGGGCGGCGA TTTCGCGAAG TTCGCGCGCA CCTACTCGCA GGACGGTTCG GCGTCGCAGG GCGGCGATCT CGGCTGGATC AGCCCGGGCG AGACCGTGCC GGAATTCGAG CGCGCGATGA ACAACCTGCA GGACGGCCAG ATCAGCCAGC CGGTGCGTAC CGAGTACGGT TACCACCTGA TCCAGGTGAT CGATCGCCGC GAGTCGGAAG GCTCGGTGCA GCAGCAGATG GATATCGCGC GTCAGGCGAT CGGCCAGCGC AAGGCCGAGC AGGCGTATGC CGACTGGCTG CGCGAACTGC GCGATTCGTC CTACGTGCAG TACAAGATCG GCGGCGTCGG CCCGGCGAAC TAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA