BuamA.17266.b

PpiC-type peptidyl-prolyl cis-trans isomerase

CENTER ID: BuamA.17266.b
ORGANISM: Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.17266.b.A2.GE31819 Mature protein( BuamA.17266.b ) 25 282
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.17266.b.A2.PS01069 Mature protein( BuamA.17266.b ) 25 282

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|398577.6.peg.5934
RefSeq: YP_001812411.1
UniProt: B1Z6T5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSHSNWKTFI AASALYLGIA CVAQAQTPAS STSPVGVVAT VNGTAITQAE VDTVLRASRQ PDTPQVRQAI KNQLIARVLI QQAAEKANYG SKPEVQTAMQ VAKANAETQL YLKDNVKPEP VTDAQIKARY DEIVASLGKD EYKPRLIVVK DAATAATVLA ELKAGKPFDG LARQYSIAPS RDAGGELPWV SFKTPAAEGK TAGLPVAIAQ ALDKLPVGAV TPESIAVDNA RAIVKLDAKR PTQVPTFNQA QATIHQQLEA LALEKASAQF VGGLLKNATI QQ
NT Sequence
ATGAGCCACT CGAACTGGAA AACTTTTATC GCCGCATCGG CGCTTTACCT CGGTATCGCC TGTGTCGCGC AAGCTCAAAC ACCCGCTTCG TCGACGTCGC CGGTCGGCGT CGTGGCAACC GTTAACGGCA CCGCGATCAC GCAGGCGGAA GTCGATACGG TGCTGCGGGC CTCGCGCCAG CCGGATACGC CGCAGGTACG CCAGGCGATC AAGAATCAGC TGATCGCCCG CGTGCTGATC CAGCAGGCCG CCGAGAAGGC GAACTACGGT AGCAAGCCGG AAGTGCAGAC GGCCATGCAG GTGGCGAAAG CGAACGCCGA GACGCAGCTG TACCTGAAGG ACAACGTGAA ACCGGAGCCG GTCACGGACG CGCAGATCAA GGCGCGCTAC GACGAGATCG TGGCGTCGCT CGGCAAGGAC GAATACAAGC CGCGCCTGAT CGTCGTGAAG GATGCCGCGA CGGCCGCGAC CGTGTTGGCC GAGTTGAAGG CGGGCAAGCC GTTCGACGGT CTCGCTCGGC AATACAGTAT CGCGCCGAGC CGCGACGCCG GCGGCGAACT GCCGTGGGTG AGCTTCAAGA CGCCGGCCGC AGAAGGGAAG ACGGCCGGGC TGCCGGTGGC GATCGCGCAG GCGCTCGACA AGCTGCCGGT CGGCGCCGTC ACGCCGGAAT CAATTGCGGT CGACAATGCG CGGGCGATCG TGAAGCTCGA CGCGAAGCGG CCGACACAGG TGCCGACGTT CAATCAGGCG CAGGCGACGA TCCACCAGCA GCTCGAGGCG CTTGCGTTGG AGAAGGCGAG CGCGCAGTTC GTCGGCGGCT TGCTGAAGAA CGCGACGATC CAACAA
Details for BuamA.17266.b.A2.GE31819
HARVESTED ON: 3/8/2011
SEQUENCED ON: 3/14/2011
EXPECTED MW: 29kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAQTPASST SPVGVVATVN GTAITQAEVD TVLRASRQPD TPQVRQAIKN QLIARVLIQQ AAEKANYGSK PEVQTAMQVA KANAETQLYL KDNVKPEPVT DAQIKARYDE IVASLGKDEY KPRLIVVKDA ATAATVLAEL KAGKPFDGLA RQYSIAPSRD AGGELPWVSF KTPAAEGKTA GLPVAIAQAL DKLPVGAVTP ESIAVDNARA IVKLDAKRPT QVPTFNQAQA TIHQQLEALA LEKASAQFVG GLLKNAT
Validated NT Sequence
atcgtcgcgt tcttcagcaa gccgccgacg aactgcgcgc tcgccttctc caacgcaagc gcctcgagct gctggtggat cgtcgcctgc gcctgattga acgtcggcac ctgtgtcggc cgcttcgcgt cgagcttcac gatcgcccgc gcattgtcga ccgcaattga ttccggcgtg acggcgccga ccggcagctt gtcgagcgcc tgcgcgatcg ccaccggcag cccggccgtc ttcccttctg cggccggcgt cttgaagctc acccacggca gttcgccgcc ggcgtcgcgg ctcggcgcga tactgtattg ccgagcgaga ccgtcgaacg gcttgcccgc cttcaactcg gccaacacgg tcgcggccgt cgcggcatcc ttcacgacga tcaggcgcgg cttgtattcg tccttgccga gcgacgccac gatctcgtcg tagcgcgcct tgatctgcgc gtccgtgacc ggctccggtt tcacgttgtc cttcaggtac agctgcgtct cggcgttcgc tttcgccacc tgcatggccg tctgcacttc cggcttgcta ccgtagttcg ccttctcggc ggcctgctgg atcagcacgc gggcgatcag ctgattcttg atcgcctggc gtacctgcgg cgtatccggc tggcgcgagg cccgcagcac cgtatcgact tccgcctgcg tgatcgcggt gccgttaacg gttgccacga cgccgaccgg cgacgtcgac gaagcgggtg tttgagccat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc cat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAQTPASST SPVGVVATVN GTAITQAEVD TVLRASRQPD TPQVRQAIKN QLIARVLIQQ AAEKANYGSK PEVQTAMQVA KANAETQLYL KDNVKPEPVT DAQIKARYDE IVASLGKDEY KPRLIVVKDA ATAATVLAEL KAGKPFDGLA RQYSIAPSRD AGGELPWVSF KTPAAEGKTA GLPVAIAQAL DKLPVGAVTP ESIAVDNARA IVKLDAKRPT QVPTFNQAQA TIHQQLEALA LEKASAQFVG GLLKNATIQQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggctcaaaca cccgcttcgt cgacgtcgcc ggtcggcgtc gtggcaaccg ttaacggcac cgcgatcacg caggcggaag tcgatacggt gctgcgggcc tcgcgccagc cggatacgcc gcaggtacgc caggcgatca agaatcagct gatcgcccgc gtgctgatcc agcaggccgc cgagaaggcg aactacggta gcaagccgga agtgcagacg gccatgcagg tggcgaaagc gaacgccgag acgcagctgt acctgaagga caacgtgaaa ccggagccgg tcacggacgc gcagatcaag gcgcgctacg acgagatcgt ggcgtcgctc ggcaaggacg aatacaagcc gcgcctgatc gtcgtgaagg atgccgcgac ggccgcgacc gtgttggccg agttgaaggc gggcaagccg ttcgacggtc tcgctcggca atacagtatc gcgccgagcc gcgacgccgg cggcgaactg ccgtgggtga gcttcaagac gccggccgca gaagggaaga cggccgggct gccggtggcg atcgcgcagg cgctcgacaa gctgccggtc ggcgccgtca cgccggaatc aattgcggtc gacaatgcgc gggcgatcgt gaagctcgac gcgaagcggc cgacacaggt gccgacgttc aatcaggcgc aggcgacgat ccaccagcag ctcgaggcgc ttgcgttgga gaaggcgagc gcgcagttcg tcggcggctt gctgaagaac gcgacgatcc aacaataaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for BuamA.17266.b.A2.PS01069
PURIFICATION DATe: 5/5/2011
CONCENTRATION: 59.7mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAQTPASST SPVGVVATVN GTAITQAEVD TVLRASRQPD TPQVRQAIKN QLIARVLIQQ AAEKANYGSK PEVQTAMQVA KANAETQLYL KDNVKPEPVT DAQIKARYDE IVASLGKDEY KPRLIVVKDA ATAATVLAEL KAGKPFDGLA RQYSIAPSRD AGGELPWVSF KTPAAEGKTA GLPVAIAQAL DKLPVGAVTP ESIAVDNARA IVKLDAKRPT QVPTFNQAQA TIHQQLEALA LEKASAQFVG GLLKNAT
Validated NT Sequence
atcgtcgcgt tcttcagcaa gccgccgacg aactgcgcgc tcgccttctc caacgcaagc gcctcgagct gctggtggat cgtcgcctgc gcctgattga acgtcggcac ctgtgtcggc cgcttcgcgt cgagcttcac gatcgcccgc gcattgtcga ccgcaattga ttccggcgtg acggcgccga ccggcagctt gtcgagcgcc tgcgcgatcg ccaccggcag cccggccgtc ttcccttctg cggccggcgt cttgaagctc acccacggca gttcgccgcc ggcgtcgcgg ctcggcgcga tactgtattg ccgagcgaga ccgtcgaacg gcttgcccgc cttcaactcg gccaacacgg tcgcggccgt cgcggcatcc ttcacgacga tcaggcgcgg cttgtattcg tccttgccga gcgacgccac gatctcgtcg tagcgcgcct tgatctgcgc gtccgtgacc ggctccggtt tcacgttgtc cttcaggtac agctgcgtct cggcgttcgc tttcgccacc tgcatggccg tctgcacttc cggcttgcta ccgtagttcg ccttctcggc ggcctgctgg atcagcacgc gggcgatcag ctgattcttg atcgcctggc gtacctgcgg cgtatccggc tggcgcgagg cccgcagcac cgtatcgact tccgcctgcg tgatcgcggt gccgttaacg gttgccacga cgccgaccgg cgacgtcgac gaagcgggtg tttgagccat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc cat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAQTPASST SPVGVVATVN GTAITQAEVD TVLRASRQPD TPQVRQAIKN QLIARVLIQQ AAEKANYGSK PEVQTAMQVA KANAETQLYL KDNVKPEPVT DAQIKARYDE IVASLGKDEY KPRLIVVKDA ATAATVLAEL KAGKPFDGLA RQYSIAPSRD AGGELPWVSF KTPAAEGKTA GLPVAIAQAL DKLPVGAVTP ESIAVDNARA IVKLDAKRPT QVPTFNQAQA TIHQQLEALA LEKASAQFVG GLLKNATIQQ
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCTCAAACA CCCGCTTCGT CGACGTCGCC GGTCGGCGTC GTGGCAACCG TTAACGGCAC CGCGATCACG CAGGCGGAAG TCGATACGGT GCTGCGGGCC TCGCGCCAGC CGGATACGCC GCAGGTACGC CAGGCGATCA AGAATCAGCT GATCGCCCGC GTGCTGATCC AGCAGGCCGC CGAGAAGGCG AACTACGGTA GCAAGCCGGA AGTGCAGACG GCCATGCAGG TGGCGAAAGC GAACGCCGAG ACGCAGCTGT ACCTGAAGGA CAACGTGAAA CCGGAGCCGG TCACGGACGC GCAGATCAAG GCGCGCTACG ACGAGATCGT GGCGTCGCTC GGCAAGGACG AATACAAGCC GCGCCTGATC GTCGTGAAGG ATGCCGCGAC GGCCGCGACC GTGTTGGCCG AGTTGAAGGC GGGCAAGCCG TTCGACGGTC TCGCTCGGCA ATACAGTATC GCGCCGAGCC GCGACGCCGG CGGCGAACTG CCGTGGGTGA GCTTCAAGAC GCCGGCCGCA GAAGGGAAGA CGGCCGGGCT GCCGGTGGCG ATCGCGCAGG CGCTCGACAA GCTGCCGGTC GGCGCCGTCA CGCCGGAATC AATTGCGGTC GACAATGCGC GGGCGATCGT GAAGCTCGAC GCGAAGCGGC CGACACAGGT GCCGACGTTC AATCAGGCGC AGGCGACGAT CCACCAGCAG CTCGAGGCGC TTGCGTTGGA GAAGGCGAGC GCGCAGTTCG TCGGCGGCTT GCTGAAGAAC GCGACGATCC AACAATAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA