SSGCID: Target Page

BuamA.17266.a

PpiC-type peptidyl-prolyl cis-trans isomerase

CENTER ID:	BuamA.17266.a
ORGANISM:	Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuamA.17266.a.A2.GE31818	Mature protein( BuamA.17266.a )		29	279

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuamA.17266.a.A2.PS01083	Mature protein( BuamA.17266.a )		29	279

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|398577.6.peg.6846
RefSeq:	YP_001816351.1
UniProt:	B1Z4Y5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRSIRTPRVP GWAALAMMAA TLHAPAASAA SDDVVATVNG VALHRVELDE AMTGRPESAR GEVLQGLVAR ELLRQAAERD GLGNAQPVRA AMQKAKVDTE NRLYVARHLP MQPVTDDEVR QRYNEIVSQI GPYQYKVSQM SFADEPTARF ALALLEKGES FANVATRSHA SDAQTSWISF RQPVKDGQTL GLPLPLAQAL ATMQPGQFTN APLHVGDRFI VARLDERRDT VIPSYTQASA AMRKAMESKR NGDAFVTFIG QLARKAEIRP AQILRSASQ

NT Sequence

ATGCGAAGCA TTCGAACGCC GCGCGTGCCA GGCTGGGCGG CACTCGCAAT GATGGCGGCG ACATTGCACG CTCCCGCCGC GTCGGCGGCT TCCGATGATG TGGTCGCGAC GGTGAACGGC GTCGCGCTGC ATCGCGTCGA ACTGGACGAA GCGATGACCG GGCGCCCCGA ATCGGCTCGC GGCGAAGTGT TGCAGGGGCT CGTCGCGCGC GAGCTGCTTC GTCAGGCAGC GGAGCGCGAC GGTTTGGGTA ACGCACAACC CGTGCGCGCC GCCATGCAAA AAGCGAAAGT CGATACGGAG AATCGGCTAT ACGTCGCCAG GCATTTGCCG ATGCAGCCGG TTACGGACGA CGAAGTTCGC CAGCGCTACA ACGAAATCGT CAGCCAGATC GGACCGTACC AATACAAGGT CAGTCAAATG TCGTTCGCCG ACGAACCGAC CGCTCGCTTT GCGCTGGCGT TGCTCGAAAA GGGTGAGTCG TTCGCGAATG TCGCGACGCG CAGCCACGCA AGCGACGCGC AGACCAGTTG GATCAGCTTC AGGCAGCCGG TGAAAGACGG CCAGACGCTC GGATTGCCGT TGCCGCTCGC GCAGGCACTC GCGACGATGC AGCCCGGCCA GTTCACGAAC GCGCCATTGC ACGTCGGTGA CCGATTCATC GTTGCGCGGC TCGACGAGCG GCGCGATACG GTCATCCCGT CGTATACGCA GGCCAGCGCC GCAATGCGCA AGGCGATGGA AAGCAAACGC AACGGTGACG CCTTCGTCAC GTTCATCGGC CAGCTTGCCC GAAAGGCCGA GATCCGGCCG GCTCAAATTC TGCGGAGTGC ATCGCAA

Details for BuamA.17266.a.A2.GE31818

HARVESTED ON:	3/8/2011
SEQUENCED ON:	3/14/2011
EXPECTED MW:	29kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMAASDDV VATVNGVALH RVELDEAMTG RPESARGEVL QGLVARELLR QAAERDGLGN AQPVRAAMQK AKVDTENRLY VARHLPMQPV TDDEVRQRYN EIVSQIGPYQ YKVSQMSFAD EPTARFALAL LEKGESFANV ATRSHASDAQ TSWISFRQPV KDGQTLGLPL PLAQALATMQ PGQFTNAPLH VGDRFIVARL DERRDTVIPS YTQASAAMRK AMESKRNGDA FVTFIGQLAR KAEIRPAQIL RSASQ

Validated NT Sequence

ttattattgc gatgcactcc gcagaatttg agccggccgg atctcggcct ttcgggcaag ctggccgatg aacgtgacga aggcgtcacc gttgcgtttg ctttccatcg ccttgcgcat tgcggcgctg gcctgcgtat acgacgggat gaccgtatcg cgccgctcgt cgagccgcgc aacgatgaat cggtcaccga cgtgcaatgg cgcgttcgtg aactggccgg gctgcatcgt cgcgagtgcc tgcgcgagcg gcaacggcaa tccgagcgtc tggccgtctt tcaccggctg cctgaagctg atccaactgg tctgcgcgtc gcttgcgtgg ctgcgcgtcg cgacattcgc gaacgactca cccttttcga gcaacgccag cgcaaagcga gcggtcggtt cgtcggcgaa cgacatttga ctgaccttgt attggtacgg tccgatctgg ctgacgattt cgttgtagcg ctggcgaact tcgtcgtccg taaccggctg catcggcaaa tgcctggcga cgtatagccg attctccgta tcgactttcg ctttttgcat ggcggcgcgc acgggttgtg cgttacccaa accgtcgcgc tccgctgcct gacgaagcag ctcgcgcgcg acgagcccct gcaacacttc gccgcgagcc gattcggggc gcccggtcat cgcttcgtcc agttcgacgc gatgcagcgc gacgccgttc accgtcgcga ccacatcatc ggaagccgcc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga ncca

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMAASDDVVA TVNGVALHRV ELDEAMTGRP ESARGEVLQG LVARELLRQA AERDGLGNAQ PVRAAMQKAK VDTENRLYVA RHLPMQPVTD DEVRQRYNEI VSQIGPYQYK VSQMSFADEP TARFALALLE KGESFANVAT RSHASDAQTS WISFRQPVKD GQTLGLPLPL AQALATMQPG QFTNAPLHVG DRFIVARLDE RRDTVIPSYT QASAAMRKAM ESKRNGDAFV TFIGQLARKA EIRPAQILRS ASQ

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcggcttcc gatgatgtgg tcgcgacggt gaacggcgtc gcgctgcatc gcgtcgaact ggacgaagcg atgaccgggc gccccgaatc ggctcgcggc gaagtgttgc aggggctcgt cgcgcgcgag ctgcttcgtc aggcagcgga gcgcgacggt ttgggtaacg cacaacccgt gcgcgccgcc atgcaaaaag cgaaagtcga tacggagaat cggctatacg tcgccaggca tttgccgatg cagccggtta cggacgacga agttcgccag cgctacaacg aaatcgtcag ccagatcgga ccgtaccaat acaaggtcag tcaaatgtcg ttcgccgacg aaccgaccgc tcgctttgcg ctggcgttgc tcgaaaaggg tgagtcgttc gcgaatgtcg cgacgcgcag ccacgcaagc gacgcgcaga ccagttggat cagcttcagg cagccggtga aagacggcca gacgctcgga ttgccgttgc cgctcgcgca ggcactcgcg acgatgcagc ccggccagtt cacgaacgcg ccattgcacg tcggtgaccg attcatcgtt gcgcggctcg acgagcggcg cgatacggtc atcccgtcgt atacgcaggc cagcgccgca atgcgcaagg cgatggaaag caaacgcaac ggtgacgcct tcgtcacgtt catcggccag cttgcccgaa aggccgagat ccggccggct caaattctgc ggagtgcatc gcaataaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa

Details for BuamA.17266.a.A2.PS01083

PURIFICATION DATe:	5/12/2011
CONCENTRATION:	36.1mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	9
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGGCTTCC GATGATGTGG TCGCGACGGT GAACGGCGTC GCGCTGCATC GCGTCGAACT GGACGAAGCG ATGACCGGGC GCCCCGAATC GGCTCGCGGC GAAGTGTTGC AGGGGCTCGT CGCGCGCGAG CTGCTTCGTC AGGCAGCGGA GCGCGACGGT TTGGGTAACG CACAACCCGT GCGCGCCGCC ATGCAAAAAG CGAAAGTCGA TACGGAGAAT CGGCTATACG TCGCCAGGCA TTTGCCGATG CAGCCGGTTA CGGACGACGA AGTTCGCCAG CGCTACAACG AAATCGTCAG CCAGATCGGA CCGTACCAAT ACAAGGTCAG TCAAATGTCG TTCGCCGACG AACCGACCGC TCGCTTTGCG CTGGCGTTGC TCGAAAAGGG TGAGTCGTTC GCGAATGTCG CGACGCGCAG CCACGCAAGC GACGCGCAGA CCAGTTGGAT CAGCTTCAGG CAGCCGGTGA AAGACGGCCA GACGCTCGGA TTGCCGTTGC CGCTCGCGCA GGCACTCGCG ACGATGCAGC CCGGCCAGTT CACGAACGCG CCATTGCACG TCGGTGACCG ATTCATCGTT GCGCGGCTCG ACGAGCGGCG CGATACGGTC ATCCCGTCGT ATACGCAGGC CAGCGCCGCA ATGCGCAAGG CGATGGAAAG CAAACGCAAC GGTGACGCCT TCGTCACGTT CATCGGCCAG CTTGCCCGAA AGGCCGAGAT CCGGCCGGCT CAAATTCTGC GGAGTGCATC GCAATAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA