SSGCID: Target Page

BuamA.17230.c

Transketolase central region

CENTER ID:	BuamA.17230.c
ORGANISM:	Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuamA.17230.c.A1.GE31814	Full length( BuamA.17230.c )		1	332

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|398577.6.peg.5120
RefSeq:	YP_001811593.1
UniProt:	B1YZH7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSTVDNKPRL KTSAMIASIA ADGQATRSAP FGHALASLAR ANGNVIGMTA DLGKYTDLHI FAKEFPERYY QMGMAEQLLM GAAAGFAHEG AQPFVTTYAV FATRRAYDFI HQAIAEDNLD VKLVCALPGL TTGYGPSHQA AEDLALMRAM PNMTVIDPCD ALDIEQMVPA IAAHKGPVYA RLLRGNVPVV LDEYDYRFEL GKAKLLRDGN DVLLISSGIM TMRALEVAKA LEADRVDVAV LHVPTIKPLD TATIVREASR KGRMVIVAEN HTVIGGLGEA VATALLGAGV AAPFRQIALP DAYLAAGALP TLHERYGISA GAMRANIRSW VG

NT Sequence

ATGAGTACCG TCGACAACAA GCCACGCCTG AAGACTTCCG CGATGATCGC GTCGATCGCG GCCGACGGGC AGGCGACGCG CTCCGCCCCG TTCGGCCATG CGCTGGCGAG TCTTGCACGC GCGAACGGCA ACGTGATCGG CATGACGGCC GATCTTGGCA AGTACACCGA CCTGCACATT TTCGCGAAGG AATTTCCGGA GCGTTACTAC CAGATGGGTA TGGCGGAGCA ATTGCTGATG GGCGCCGCCG CCGGGTTCGC GCACGAAGGT GCGCAGCCGT TCGTCACGAC CTATGCGGTG TTCGCGACAC GCCGGGCATA CGACTTCATT CACCAGGCGA TTGCCGAGGA CAATCTCGAT GTGAAGCTGG TGTGTGCGTT GCCGGGACTC ACGACCGGCT ACGGGCCGAG CCATCAAGCG GCAGAGGACC TCGCGCTGAT GCGTGCGATG CCGAACATGA CGGTGATCGA TCCGTGCGAC GCGCTCGACA TCGAGCAGAT GGTGCCGGCG ATTGCCGCGC ACAAGGGGCC GGTCTATGCG CGCCTGCTGC GTGGGAACGT GCCGGTCGTG CTCGACGAAT ACGATTACCG TTTCGAACTC GGCAAGGCCA AGCTGCTGCG CGACGGCAAC GACGTGCTGT TGATCTCGTC CGGGATCATG ACGATGCGCG CGCTCGAGGT TGCGAAGGCG CTTGAGGCGG ATCGCGTCGA TGTGGCCGTG CTGCACGTGC CGACGATCAA GCCGCTCGAT ACGGCGACCA TCGTTCGCGA GGCATCGCGC AAGGGACGCA TGGTGATCGT CGCGGAGAAC CATACGGTGA TCGGCGGGCT CGGCGAAGCG GTGGCGACTG CATTGCTCGG CGCCGGCGTT GCCGCACCGT TCCGCCAGAT CGCGCTGCCG GATGCGTATC TCGCCGCGGG CGCGTTGCCG ACGCTGCACG AGCGCTATGG GATCTCGGCC GGCGCGATGC GCGCGAATAT CAGATCGTGG GTCGGA

Details for BuamA.17230.c.A1.GE31814

HARVESTED ON:	3/8/2011
SEQUENCED ON:	3/14/2011
EXPECTED MW:	37kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	88
PERCENT COVERAGE:	103

Validated AA Sequence

MGTLEAQTQG PGSMSTVDNK PRLKTSAMIA SIAADGQATR SAPFGHALAS LARANGNVIG MTADLGKYTD LHIFAKEFPE RYYQMGMAEQ LLMGAAAGFA HEGAQPFVTT YAVFATRRAY DFIHQAIAED NLDVKLVCAL PGLTTGYGPS HQAAEDLALM RAMPNMTVID PCDALDIEQM VPAIAAHKGP VYARLLRGNV PVVLDEYDYR FELGKAKLLR DGNDVLLISS GIMTMRALEV AKALXADRVD VAVLHVPTIK PLDTATIVRE ASRKGRMVIV AXNHTXIGGL GEAXAXALLX AXXAAPXXXI ALXXAXXXXX XLPXLXXXXX XXXXXXXXXX XXXXXXXXXX XXAXXXXXXX XXXXX

Validated NT Sequence

cnnnnnnnnn nnnnnnnnnn gcnnnnnncc atgtnnncnn nnntnccntg nnnnnnnnnn ntcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atgagtaccg tcgacaacaa gccacgcctg aagacttccg cgatgatcgc gtcgatcgcg gccgacgggc aggcgacgcg ctccgccccg ttcggccatg cgctggcgag tcttgcacgc gcgaacggca acgtgatcgg catgacggcc gatcttggca agtacaccga cctgcacatt ttcgcgaagg aatttccgga gcgttactac cagatgggta tggcggagca attgctgatg ggcgccgccg ccgggttcgc gcacgaaggt gcgcagccgt tcgtcacgac ctatgcggtg ttcgcgacac gccgggcata cgacttcatt caccaggcga ttgccgagga caatctcgat gtgaagctgg tgtgtgcgtt gccgggactc acgaccggct acgggccgag ccatcaagcg gcagaggacc tcgcgctgat gcgtgcgatg ccgaacatga cggtgatcga tccgtgcgac gcgctcgaca tcgagcagat ggtgccggcg attgccgcgc acaaggggcc ggtctatgcg cgcctgctgc gtgggaacgt gccggtcgtg ctcgacgaat acgattaccg tttcgaactc ggcaaggcca agctgctgcg cgacggcaac gacgtgctgt tgatctcgtc cgggatcatg acgatgcgcg cgctcgaggt tgcgaaggcg cttgangcgg atcgcgtcga tgtggccgtg ctgcacgtgc cgacgatcaa gccgctcgat acggcgacca tcgttcgcga ggcatcgcgc aagggacgca tggtgatcgt cgcgganaac catacggnga tcggcgggct cggcgaagcg gnggcgnctg cattgctcng cgcnnnnntt gccgcaccgn tcnnncnnat cgcnctgnnn nntgcgtann nnnccncggn cncnttgccn annctgcncg ancnctnnng ggnnnnnnnn nnnnnatgnn nncnnnntnn nancntgngg nngnnannna nnannnnnnn nnnttnngcn nnnnnnttnt nnnnnnnnnn nnnnnnnnnn nnnnngnng

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSTVDNKPR LKTSAMIASI AADGQATRSA PFGHALASLA RANGNVIGMT ADLGKYTDLH IFAKEFPERY YQMGMAEQLL MGAAAGFAHE GAQPFVTTYA VFATRRAYDF IHQAIAEDNL DVKLVCALPG LTTGYGPSHQ AAEDLALMRA MPNMTVIDPC DALDIEQMVP AIAAHKGPVY ARLLRGNVPV VLDEYDYRFE LGKAKLLRDG NDVLLISSGI MTMRALEVAK ALEADRVDVA VLHVPTIKPL DTATIVREAS RKGRMVIVAE NHTVIGGLGE AVATALLGAG VAAPFRQIAL PDAYLAAGAL PTLHERYGIS AGAMRANIRS WVG

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagtaccgtc gacaacaagc cacgcctgaa gacttccgcg atgatcgcgt cgatcgcggc cgacgggcag gcgacgcgct ccgccccgtt cggccatgcg ctggcgagtc ttgcacgcgc gaacggcaac gtgatcggca tgacggccga tcttggcaag tacaccgacc tgcacatttt cgcgaaggaa tttccggagc gttactacca gatgggtatg gcggagcaat tgctgatggg cgccgccgcc gggttcgcgc acgaaggtgc gcagccgttc gtcacgacct atgcggtgtt cgcgacacgc cgggcatacg acttcattca ccaggcgatt gccgaggaca atctcgatgt gaagctggtg tgtgcgttgc cgggactcac gaccggctac gggccgagcc atcaagcggc agaggacctc gcgctgatgc gtgcgatgcc gaacatgacg gtgatcgatc cgtgcgacgc gctcgacatc gagcagatgg tgccggcgat tgccgcgcac aaggggccgg tctatgcgcg cctgctgcgt gggaacgtgc cggtcgtgct cgacgaatac gattaccgtt tcgaactcgg caaggccaag ctgctgcgcg acggcaacga cgtgctgttg atctcgtccg ggatcatgac gatgcgcgcg ctcgaggttg cgaaggcgct tgaggcggat cgcgtcgatg tggccgtgct gcacgtgccg acgatcaagc cgctcgatac ggcgaccatc gttcgcgagg catcgcgcaa gggacgcatg gtgatcgtcg cggagaacca tacggtgatc ggcgggctcg gcgaagcggt ggcgactgca ttgctcggcg ccggcgttgc cgcaccgttc cgccagatcg cgctgccgga tgcgtatctc gccgcgggcg cgttgccgac gctgcacgag cgctatggga tctcggccgg cgcgatgcgc gcgaatatca gatcgtgggt cggataaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa