SSGCID: Target Page

BuamA.17230.a

Transketolase central region

CENTER ID:	BuamA.17230.a
ORGANISM:	Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuamA.17230.a.A1.GE31812	Full length( BuamA.17230.a )		1	305

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuamA.17230.a.A1.PS01104	Full length( BuamA.17230.a )		1	305

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|398577.6.peg.2350
RefSeq:	YP_001808982.1
UniProt:	B1YUC6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRDAFSDAIV GAARQDPKVL LLTGDHGYAL FDAFRKACPD QYINCGIAEQ NMVGVAAGLA KAGFKPIVYG LAAFVPIRVL EQIKIDVCYE NLPVTFIGDG AGVVYAQLGT SHQSTEDIAA LRAIPQLSIY SPADRFELTA CMQDISTRQT PAYLRMGKAD LGDVHPGSLD SWCHGDAVPL TNEAHSSLFI ATGSGVKLAQ KVGAAIGGAD IVSVPCIKPF PVDSIRRLAA GRQAIVTFEE HSIYGGLGGA VAETLMGSFV GAFVRVGIQD RFSTLCGNYA YLMESHGLSP DAVTEQIKKA LDAGV

NT Sequence

ATGAGGGATG CGTTTTCCGA TGCCATCGTG GGTGCGGCGC GGCAAGATCC CAAGGTTCTT CTGCTGACCG GCGATCACGG CTATGCGCTC TTCGATGCAT TTCGCAAAGC ATGTCCCGAT CAGTACATCA ATTGCGGGAT TGCCGAGCAG AATATGGTGG GGGTGGCAGC GGGTCTCGCC AAGGCTGGAT TCAAACCCAT CGTCTACGGG CTCGCCGCAT TCGTGCCCAT TCGCGTCCTG GAACAGATCA AGATCGATGT CTGCTATGAA AATCTGCCGG TGACGTTCAT CGGCGATGGC GCCGGCGTCG TCTACGCACA ACTGGGTACG AGCCACCAAA GTACGGAGGA CATCGCTGCG CTTCGCGCGA TCCCGCAATT GTCGATCTAT TCTCCTGCCG ACCGGTTCGA ATTGACGGCA TGCATGCAGG ACATCTCGAC CAGACAGACG CCTGCCTATC TGCGAATGGG CAAGGCGGAT CTTGGCGATG TGCATCCCGG ATCGCTCGAC TCCTGGTGCC ATGGCGATGC CGTCCCGTTG ACGAACGAAG CGCACTCGAG CCTTTTCATT GCGACAGGTT CGGGCGTGAA GCTGGCTCAG AAGGTCGGTG CTGCTATCGG TGGCGCGGAC ATCGTCTCCG TGCCCTGCAT CAAACCCTTT CCTGTCGATT CGATCCGCAG GCTCGCGGCG GGCAGGCAAG CGATCGTGAC GTTTGAAGAG CATTCGATTT ACGGTGGGCT GGGTGGCGCC GTCGCGGAGA CGCTGATGGG CAGTTTTGTC GGGGCGTTCG TGCGTGTGGG CATTCAAGAC AGGTTCAGTA CGTTGTGTGG CAACTATGCC TATCTGATGG AAAGCCATGG CCTTTCGCCG GATGCCGTCA CAGAGCAAAT AAAAAAGGCA CTCGATGCAG GCGTC

Details for BuamA.17230.a.A1.GE31812

HARVESTED ON:	3/8/2011
SEQUENCED ON:	3/14/2011
EXPECTED MW:	34kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMRDAFSD AIVGAARQDP KVLLLTGDHG YALFDAFRKA CPDQYINCGI AEQNMVGVAA GLAKAGFKPI VYGLAAFVPI RVLEQIKIDV CYENLPVTFI GDGAGVVYAQ LGTSHQSTED IAALRAIPQL SIYSPADRFE LTACMQDIST RQTPAYLRMG KADLGDVHPG SLDSWCHGDA VPLTNEAHSS LFIATGSGVK LAQKVGAAIG GADIVSVPCI KPFPVDSIRR LAAGRQAIVT FEEHSIYGGL GGAVAETLMG SFVGAFVRVG IQDRFSTLCG NYAYLMESHG LSPDAVTEQI KKALDAGV

Validated NT Sequence

ttattagacg cctgcatcga gtgccttttt tatttgctct gtgacggcat ccggcgaaag gccatggctt tccatcagat aggcatagtt gccacacaac gtactgaacc tgtcttgaat gcccacacgc acgaacgccc cgacaaaact gcccatcagc gtctccgcga cggcgccacc cagcccaccg taaatcgaat gctcttcaaa cgtcacgatc gcttgcctgc ccgccgcgag cctgcggatc gaatcgacag gaaagggttt gatgcagggc acggagacga tgtccgcgcc accgatagca gcaccgacct tctgagccag cttcacgccc gaacctgtcg caatgaaaag gctcgagtgc gcttcgttcg tcaacgggac ggcatcgcca tggcaccagg agtcgagcga tccgggatgc acatcgccaa gatccgcctt gcccattcgc agataggcag gcgtctgtct ggtcgagatg tcctgcatgc atgccgtcaa ttcgaaccgg tcggcaggag aatagatcga caattgcggg atcgcgcgaa gcgcagcgat gtcctccgta ctttggtggc tcgtacccag ttgtgcgtag acgacgccgg cgccatcgcc gatgaacgtc accggcagat tttcatagca gacatcgatc ttgatctgtt ccaggacgcg aatgggcacg aatgcggcga gcccgtagac gatgggtttg aatccagcct tggcgagacc cgctgccacc cccaccatat tctgctcggc aatcccgcaa ttgatgtact gatcgggaca tgctttgcga aatgcatcga agagcgcata gccgtgatcg ccggtcagca gaagaacctt gggatcttgc cgcgccgcac ccacgatggc atcggaaaac gcatccctca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggng

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMRDAFSDAI VGAARQDPKV LLLTGDHGYA LFDAFRKACP DQYINCGIAE QNMVGVAAGL AKAGFKPIVY GLAAFVPIRV LEQIKIDVCY ENLPVTFIGD GAGVVYAQLG TSHQSTEDIA ALRAIPQLSI YSPADRFELT ACMQDISTRQ TPAYLRMGKA DLGDVHPGSL DSWCHGDAVP LTNEAHSSLF IATGSGVKLA QKVGAAIGGA DIVSVPCIKP FPVDSIRRLA AGRQAIVTFE EHSIYGGLGG AVAETLMGSF VGAFVRVGIQ DRFSTLCGNY AYLMESHGLS PDAVTEQIKK ALDAGV

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagggatgcg ttttccgatg ccatcgtggg tgcggcgcgg caagatccca aggttcttct gctgaccggc gatcacggct atgcgctctt cgatgcattt cgcaaagcat gtcccgatca gtacatcaat tgcgggattg ccgagcagaa tatggtgggg gtggcagcgg gtctcgccaa ggctggattc aaacccatcg tctacgggct cgccgcattc gtgcccattc gcgtcctgga acagatcaag atcgatgtct gctatgaaaa tctgccggtg acgttcatcg gcgatggcgc cggcgtcgtc tacgcacaac tgggtacgag ccaccaaagt acggaggaca tcgctgcgct tcgcgcgatc ccgcaattgt cgatctattc tcctgccgac cggttcgaat tgacggcatg catgcaggac atctcgacca gacagacgcc tgcctatctg cgaatgggca aggcggatct tggcgatgtg catcccggat cgctcgactc ctggtgccat ggcgatgccg tcccgttgac gaacgaagcg cactcgagcc ttttcattgc gacaggttcg ggcgtgaagc tggctcagaa ggtcggtgct gctatcggtg gcgcggacat cgtctccgtg ccctgcatca aaccctttcc tgtcgattcg atccgcaggc tcgcggcggg caggcaagcg atcgtgacgt ttgaagagca ttcgatttac ggtgggctgg gtggcgccgt cgcggagacg ctgatgggca gttttgtcgg ggcgttcgtg cgtgtgggca ttcaagacag gttcagtacg ttgtgtggca actatgccta tctgatggaa agccatggcc tttcgccgga tgccgtcaca gagcaaataa aaaaggcact cgatgcaggc gtctaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa

Details for BuamA.17230.a.A1.PS01104

PURIFICATION DATe:	5/31/2011
CONCENTRATION:	13.23mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	5
VIAL VOLUME:	100µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGGGATGCG TTTTCCGATG CCATCGTGGG TGCGGCGCGG CAAGATCCCA AGGTTCTTCT GCTGACCGGC GATCACGGCT ATGCGCTCTT CGATGCATTT CGCAAAGCAT GTCCCGATCA GTACATCAAT TGCGGGATTG CCGAGCAGAA TATGGTGGGG GTGGCAGCGG GTCTCGCCAA GGCTGGATTC AAACCCATCG TCTACGGGCT CGCCGCATTC GTGCCCATTC GCGTCCTGGA ACAGATCAAG ATCGATGTCT GCTATGAAAA TCTGCCGGTG ACGTTCATCG GCGATGGCGC CGGCGTCGTC TACGCACAAC TGGGTACGAG CCACCAAAGT ACGGAGGACA TCGCTGCGCT TCGCGCGATC CCGCAATTGT CGATCTATTC TCCTGCCGAC CGGTTCGAAT TGACGGCATG CATGCAGGAC ATCTCGACCA GACAGACGCC TGCCTATCTG CGAATGGGCA AGGCGGATCT TGGCGATGTG CATCCCGGAT CGCTCGACTC CTGGTGCCAT GGCGATGCCG TCCCGTTGAC GAACGAAGCG CACTCGAGCC TTTTCATTGC GACAGGTTCG GGCGTGAAGC TGGCTCAGAA GGTCGGTGCT GCTATCGGTG GCGCGGACAT CGTCTCCGTG CCCTGCATCA AACCCTTTCC TGTCGATTCG ATCCGCAGGC TCGCGGCGGG CAGGCAAGCG ATCGTGACGT TTGAAGAGCA TTCGATTTAC GGTGGGCTGG GTGGCGCCGT CGCGGAGACG CTGATGGGCA GTTTTGTCGG GGCGTTCGTG CGTGTGGGCA TTCAAGACAG GTTCAGTACG TTGTGTGGCA ACTATGCCTA TCTGATGGAA AGCCATGGCC TTTCGCCGGA TGCCGTCACA GAGCAAATAA AAAAGGCACT CGATGCAGGC GTCTAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA