SSGCID: Target Page

BuamA.16004.a

Beta-lactamase domain protein

CENTER ID:	BuamA.16004.a
ORGANISM:	Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuamA.16004.a.B1.GE39264	Full length( BuamA.16004.a )		1	298

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|398577.6.peg.5979
RefSeq:	YP_001815523.1
UniProt:	B1Z5C4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLNASPGWID QRLALVGTAD VPLYVIVNDE AATLIEGGLS GMTELVWQQL HDLLRDFGGI RHLRYWLITH SHYDHCSLLM TLKSRMPWLH VSGSPDAFDA FQSPSACRTI RQLDAQASRS WDPAVGVDFT ELSDLPFYPV NPDTQLDIGD GMQIRTIALP GHSRCQFGYY CPQLDIGFVS DALGEFQGAT SWLPLVFQDL FAYRHTLDVI EQLQAPRLAL GHHGILTGEL ARSAVRHARA CLDAREADAR AVRGNATATH ELAHHWTARY AARSERVVPH FLHLKSMMHM IDLFHRAE

NT Sequence

ATGCTGAACG CTTCCCCGGG GTGGATCGAT CAGCGGCTGG CCCTCGTCGG CACGGCCGAC GTGCCGCTGT ACGTGATCGT CAACGACGAG GCCGCGACGC TGATCGAGGG TGGCCTGAGC GGCATGACGG AGCTCGTGTG GCAGCAGCTT CACGACCTGC TGCGGGACTT TGGCGGCATC CGGCATTTGC GCTACTGGCT GATCACCCAC TCGCACTATG ACCATTGCAG CCTGCTGATG ACGCTCAAGT CGCGCATGCC GTGGCTCCAT GTGTCCGGTT CTCCCGACGC GTTCGATGCG TTCCAGAGCC CGTCGGCCTG CCGAACCATC CGTCAGCTCG ACGCGCAAGC GTCACGGTCG TGGGATCCCG CCGTCGGCGT CGATTTCACC GAGTTATCCG ACCTGCCGTT CTACCCGGTC AATCCGGACA CACAGCTCGA CATCGGCGAC GGGATGCAGA TTCGCACGAT CGCGCTGCCG GGCCACAGCC GTTGCCAGTT CGGCTACTAC TGCCCGCAGC TGGACATCGG TTTCGTGTCG GATGCGCTCG GCGAATTTCA GGGCGCCACC AGCTGGCTGC CGCTGGTCTT TCAGGACCTG TTTGCGTATC GGCACACGCT GGACGTCATC GAGCAACTGC AGGCGCCGCG GCTCGCATTG GGACATCACG GCATCCTCAC CGGCGAGCTC GCCCGATCGG CCGTGCGGCA TGCACGTGCG TGCCTCGATG CGCGCGAGGC CGACGCACGC GCGGTTCGCG GCAACGCCAC CGCCACGCAC GAACTGGCCC ACCACTGGAC TGCGCGTTAT GCGGCAAGGA GCGAACGGGT GGTGCCGCAT TTCCTGCACC TCAAAAGCAT GATGCACATG ATCGATCTGT TCCATCGCGC CGAA

Details for BuamA.16004.a.B1.GE39264

HARVESTED ON:	6/10/2015
SEQUENCED ON:	6/5/2015
EXPECTED MW:	34kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	97
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHML NASPGWIDQR LALVGTADVP LYVIVNDEAA TLIEGGLSGM TELVWQQLHD LLRDFGGIRH LRYWLITHSH YDHCSLLMTL KSRMPWLHVS GSPDAFDAFQ SPSACRTIRQ LDAQASRSWD PAVGVDFTEL SDLPFYPVNP DTQLDIGDGM QIRTIALPGH SRCQFGYYCP QLDIGFVSDA LGEFQGATSW LPLVFQDLFA YRHTLDVIEQ LQAPRLALGH HGILTGELAR SAVRHARACL DAREADARAV RGNATATHEL AHHWTARYAA RSERVVPHFL HLKSMMHXXX XVPSR

Validated NT Sequence

ggcgcgatgg aacannnnnn nnnatgtgca tcatgctttt gaggtgcagg aaatgcggca ccacccgttc gctccttgcc gcataacgcg cagtccagtg gtgggccagt tcgtgcgtgg cggtggcgtt gccgcgaacc gcgcgtgcgt cggcctcgcg cgcatcgagg cacgcacgtg catgccgcac ggccgatcgg gcgagctcgc cggtgaggat gccgtgatgt cccaatgcga gccgcggcgc ctgcagttgc tcgatgacgt ccagcgtgtg ccgatacgca aacaggtcct gaaagaccag cggcagccag ctggtggcgc cctgaaattc gccgagcgca tccgacacga aaccgatgtc cagctgcggg cagtagtagc cgaactggca acggctgtgg cccggcagcg cgatcgtgcg aatctgcatc ccgtcgccga tgtcgagctg tgtgtccgga ttgaccgggt agaacggcag gtcggataac tcggtgaaat cgacgccgac ggcgggatcc cacgaccgtg acgcttgcgc gtcgagctga cggatggttc ggcaggccga cgggctctgg aacgcatcga acgcgtcggg agaaccggac acatggagcc acggcatgcg cgacttgagc gtcatcagca ggctgcaatg gtcatagtgc gagtgggtga tcagccagta gcgcaaatgc cggatgccgc caaagtcccg cagcaggtcg tgaagctgct gccacacgag ctccgtcatg ccgctcaggc caccctcgat cagcgtcgcg gcctcgtcgt tgacgatcac gtacagcggc acgtcggccg tgccgacgag ggccagccgc tgatcgatcc accccgggga agcgttcagc atatggtggt ggtggtggtg agccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctga acgcttcccc ggggtggatc gatcagcggc tggccctcgt cggcacggcc gacgtgccgc tgtacgtgat cgtcaacgac gaggccgcga cgctgatcga gggtggcctg agcggcatga cggagctcgt gtggcagcag cttcacgacc tgctgcggga ctttggcggc atccggcatt tgcgctactg gctgatcacc cactcgcact atgaccattg cagcctgctg atgacgctca agtcgcgcat gccgtggctc catgtgtccg gttctcccga cgcgttcgat gcgttccaga gcccgtcggc ctgccgaacc atccgtcagc tcgacgcgca agcgtcacgg tcgtgggatc ccgccgtcgg cgtcgatttc accgagttat ccgacctgcc gttctacccg gtcaatccgg acacacagct cgacatcggc gacgggatgc agattcgcac gatcgcgctg ccgggccaca gccgttgcca gttcggctac tactgcccgc agctggacat cggtttcgtg tcggatgcgc tcggcgaatt tcagggcgcc accagctggc tgccgctggt ctttcaggac ctgtttgcgt atcggcacac gctggacgtc atcgagcaac tgcaggcgcc gcggctcgca ttgggacatc acggcatcct caccggcgag ctcgcccgat cggccgtgcg gcatgcacgt gcgtgcctcg atgcgcgcga ggccgacgca cgcgcggttc gcggcaacgc caccgccacg cacgaactgg cccaccactg gactgcgcgt tatgcggcaa ggagcgaacg ggtggtgccg catttcctgc acctcaaaag catgatgcac atgatcgatc tgttccatcg cgccgaatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at