BuamA.15997.a
Beta-lactamase domain protein
| CENTER ID: | BuamA.15997.a |
| ORGANISM: | Burkholderia ambifaria MC40-6 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuamA.15997.a.B1.GE39262 | Full length( BuamA.15997.a ) | 1 | 358 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuamA.15997.a.B1.PW37786 | Full length( BuamA.15997.a ) | 1 | 358 |
Structures
5I0P
DEPOSITED: 2/4/2016
DETERMINATION: XRay
CLONE: BuamA.15997.a.B1.GE39262
PROTEIN: BuamA.15997.a.B1.PW37786
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_155546 |
| PATRIC ID: | fig|398577.6.peg.2929 |
| RefSeq: | YP_001809529.1 |
| UniProt: | B1YNR3 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNALEHQLDY PFADTLPAAG DTFEVAPGVR WLRMPLPFSL DHINLWLLRD EIDGQAGWTI VDCGISSEAI RTHWEQIFDR HLDGLPVLRV LVTHCHPDHF GLANWLCEGG DQGRWNVRLW MTLGEYMFGC LMAAGNGSNA GGAAAADHFA RHGLTDPAAL DKLRSRRSYY SDLVPAVPPR YRRLREGDAV KIGARTWRVV TGYGHSPEHC ALHSEADGVL ISGDMVLPRI STNVSVFDLE PEANPLALYL ESLGRYETMA PDTLVLPSHG KPFRGVRTRI AQLRAHHDAR LEEVRVACAE KPMSAAGIVP IMFRRRELDI HQMTFALGEA LAHLNLLWLA GELVREHGDD GVIRYARA
NT Sequence
ATGAACGCAC TGGAACACCA ACTCGACTAT CCCTTCGCCG ACACGCTGCC CGCCGCGGGC GACACGTTCG AGGTCGCACC CGGCGTGCGC TGGCTGCGCA TGCCGCTGCC GTTTTCGCTC GATCACATCA ATCTCTGGCT GCTGCGCGAC GAGATCGACG GGCAGGCCGG CTGGACGATC GTCGACTGCG GGATCTCGTC GGAGGCGATC CGTACGCACT GGGAACAGAT CTTCGATCGG CATCTCGACG GGCTGCCGGT GCTGCGCGTG CTCGTCACGC ACTGCCACCC CGATCACTTC GGCCTCGCGA ACTGGCTGTG CGAAGGCGGT GACCAAGGCC GCTGGAACGT GCGGCTGTGG ATGACGCTCG GCGAATACAT GTTCGGCTGC CTGATGGCGG CGGGCAACGG CTCGAACGCC GGCGGCGCGG CCGCGGCCGA TCACTTCGCG CGCCACGGCT TGACCGATCC GGCCGCGCTC GACAAGCTGC GCAGCCGCCG CAGCTACTAC TCGGATCTCG TGCCGGCCGT GCCGCCGCGC TACCGGCGCC TGCGCGAAGG CGACGCGGTG AAGATCGGCG CGCGCACCTG GCGCGTCGTC ACCGGCTACG GCCATTCGCC TGAACATTGC GCGCTGCACA GCGAAGCGGA CGGCGTGCTG ATCTCCGGCG ACATGGTGCT GCCGCGCATC TCGACCAACG TGTCCGTGTT CGATCTCGAA CCGGAAGCGA ACCCGCTCGC GCTGTACCTC GAGTCGCTCG GCCGCTACGA AACGATGGCG CCCGACACGC TCGTGCTGCC GTCGCACGGC AAGCCGTTCC GCGGCGTGCG CACGCGCATC GCGCAACTGC GCGCGCACCA CGACGCGCGG CTCGAGGAAG TGCGCGTCGC GTGCGCGGAG AAGCCGATGA GTGCGGCCGG CATCGTGCCG ATCATGTTCC GCCGCCGCGA GCTCGACATC CACCAGATGA CGTTCGCGCT CGGCGAGGCG CTCGCCCACC TGAACCTGCT GTGGCTCGCG GGTGAGCTCG TGCGCGAGCA CGGTGACGAC GGCGTGATTC GCTACGCGAG GGCC
Details for BuamA.15997.a.B1.GE39262
| HARVESTED ON: | 6/10/2015 |
| SEQUENCED ON: | 6/5/2015 |
| EXPECTED MW: | 41kDa |
| OBSERVED MW: | 39kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHMN ALEHQLDYPF ADTLPAAGDT FEVAPGVRWL RMPLPFSLDH INLWLLRDEI DGQAGWTIVD CGISSEAIRT HWEQIFDRHL DGLPVLRVLV THCHPDHFGL ANWLCEGGDQ GRWNVRLWMT LGEYMFGCLM AAGNGSNAGG AAAADHFARH GLTDPAALDK LRSRRSYYSD LVPAVPPRYR RLREGDAVKI GARTWRVVTG YGHSPEHCAL HSEADGVLIS GDMVLPRIST NVSVFDLEPE ANPLALYLES LGRYETMAPD TLVLPSHGKP FRGVRTRIAQ LRAHHDARLE EVRVACAEKP MSAAGIVPIM FRRRELDIHQ MTFALGEALA HLNLLWLAGE LVREHGD
Validated NT Sequence
cgtcaccgtg ctcgcgcacg agctcacccg cgagccacag caggttcagg tgggcgagcg cctcgccgag cgcgaacgtc atctggtgga tgtcgagctc gcggcggcgg aacatgatcg gcacgatgcc ggccgcactc atcggcttct ccgcgcacgc gacgcgcact tcctcgagcc gcgcgtcgtg gtgcgcgcgc agttgcgcga tgcgcgtgcg cacgccgcgg aacggcttgc cgtgcgacgg cagcacgagc gtgtcgggcg ccatcgtttc gtagcggccg agcgactcga ggtacagcgc gagcgggttc gcttccggtt cgagatcgaa cacggacacg ttggtcgaga tgcgcggcag caccatgtcg ccggagatca gcacgccgtc cgcttcgctg tgcagcgcgc aatgttcagg cgaatggccg tagccggtga cgacgcgcca ggtgcgcgcg ccgatcttca ccgcgtcgcc ttcgcgcagg cgccggtagc gcggcggcac ggccggcacg agatccgagt agtagctgcg gcggctgcgc agcttgtcga gcgcggccgg atcggtcaag ccgtggcgcg cgaagtgatc ggccgcggcc gcgccgccgg cgttcgagcc gttgcccgcc gccatcaggc agccgaacat gtattcgccg agcgtcatcc acagccgcac gttccagcgg ccttggtcac cgccttcgca cagccagttc gcgaggccga agtgatcggg gtggcagtgc gtgacgagca cgcgcagcac cggcagcccg tcgagatgcc gatcgaagat ctgttcccag tgcgtacgga tcgcctccga cgagatcccg cagtcgacga tcgtccagcc ggcctgcccg tcgatctcgt cgcgcagcag ccagagattg atgtgatcga gcgaaaacgg cagcggcatg cgcagccagc gcacgccggg tgcgacctcg aacgtgtcgc ccgcggcggg cagcgtgtcg gcgaagggat agtcgagttg gtgttccagt gcgttcatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHMN ALEHQLDYPF ADTLPAAGDT FEVAPGVRWL RMPLPFSLDH INLWLLRDEI DGQAGWTIVD CGISSEAIRT HWEQIFDRHL DGLPVLRVLV THCHPDHFGL ANWLCEGGDQ GRWNVRLWMT LGEYMFGCLM AAGNGSNAGG AAAADHFARH GLTDPAALDK LRSRRSYYSD LVPAVPPRYR RLREGDAVKI GARTWRVVTG YGHSPEHCAL HSEADGVLIS GDMVLPRIST NVSVFDLEPE ANPLALYLES LGRYETMAPD TLVLPSHGKP FRGVRTRIAQ LRAHHDARLE EVRVACAEKP MSAAGIVPIM FRRRELDIHQ MTFALGEALA HLNLLWLAGE LVREHGDDGV IRYARA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacg cactggaaca ccaactcgac tatcccttcg ccgacacgct gcccgccgcg ggcgacacgt tcgaggtcgc acccggcgtg cgctggctgc gcatgccgct gccgttttcg ctcgatcaca tcaatctctg gctgctgcgc gacgagatcg acgggcaggc cggctggacg atcgtcgact gcgggatctc gtcggaggcg atccgtacgc actgggaaca gatcttcgat cggcatctcg acgggctgcc ggtgctgcgc gtgctcgtca cgcactgcca ccccgatcac ttcggcctcg cgaactggct gtgcgaaggc ggtgaccaag gccgctggaa cgtgcggctg tggatgacgc tcggcgaata catgttcggc tgcctgatgg cggcgggcaa cggctcgaac gccggcggcg cggccgcggc cgatcacttc gcgcgccacg gcttgaccga tccggccgcg ctcgacaagc tgcgcagccg ccgcagctac tactcggatc tcgtgccggc cgtgccgccg cgctaccggc gcctgcgcga aggcgacgcg gtgaagatcg gcgcgcgcac ctggcgcgtc gtcaccggct acggccattc gcctgaacat tgcgcgctgc acagcgaagc ggacggcgtg ctgatctccg gcgacatggt gctgccgcgc atctcgacca acgtgtccgt gttcgatctc gaaccggaag cgaacccgct cgcgctgtac ctcgagtcgc tcggccgcta cgaaacgatg gcgcccgaca cgctcgtgct gccgtcgcac ggcaagccgt tccgcggcgt gcgcacgcgc atcgcgcaac tgcgcgcgca ccacgacgcg cggctcgagg aagtgcgcgt cgcgtgcgcg gagaagccga tgagtgcggc cggcatcgtg ccgatcatgt tccgccgccg cgagctcgac atccaccaga tgacgttcgc gctcggcgag gcgctcgccc acctgaacct gctgtggctc gcgggtgagc tcgtgcgcga gcacggtgac gacggcgtga ttcgctacgc gagggcctga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for BuamA.15997.a.B1.PW37786
| PURIFICATION DATe: | 9/16/2015 |
| CONCENTRATION: | 26mg/ml |
| OBSERVED MW: | 39kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 9 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMN ALEHQLDYPF ADTLPAAGDT FEVAPGVRWL RMPLPFSLDH INLWLLRDEI DGQAGWTIVD CGISSEAIRT HWEQIFDRHL DGLPVLRVLV THCHPDHFGL ANWLCEGGDQ GRWNVRLWMT LGEYMFGCLM AAGNGSNAGG AAAADHFARH GLTDPAALDK LRSRRSYYSD LVPAVPPRYR RLREGDAVKI GARTWRVVTG YGHSPEHCAL HSEADGVLIS GDMVLPRIST NVSVFDLEPE ANPLALYLES LGRYETMAPD TLVLPSHGKP FRGVRTRIAQ LRAHHDARLE EVRVACAEKP MSAAGIVPIM FRRRELDIHQ MTFALGEALA HLNLLWLAGE LVREHGD
Validated NT Sequence
cgtcaccgtg ctcgcgcacg agctcacccg cgagccacag caggttcagg tgggcgagcg cctcgccgag cgcgaacgtc atctggtgga tgtcgagctc gcggcggcgg aacatgatcg gcacgatgcc ggccgcactc atcggcttct ccgcgcacgc gacgcgcact tcctcgagcc gcgcgtcgtg gtgcgcgcgc agttgcgcga tgcgcgtgcg cacgccgcgg aacggcttgc cgtgcgacgg cagcacgagc gtgtcgggcg ccatcgtttc gtagcggccg agcgactcga ggtacagcgc gagcgggttc gcttccggtt cgagatcgaa cacggacacg ttggtcgaga tgcgcggcag caccatgtcg ccggagatca gcacgccgtc cgcttcgctg tgcagcgcgc aatgttcagg cgaatggccg tagccggtga cgacgcgcca ggtgcgcgcg ccgatcttca ccgcgtcgcc ttcgcgcagg cgccggtagc gcggcggcac ggccggcacg agatccgagt agtagctgcg gcggctgcgc agcttgtcga gcgcggccgg atcggtcaag ccgtggcgcg cgaagtgatc ggccgcggcc gcgccgccgg cgttcgagcc gttgcccgcc gccatcaggc agccgaacat gtattcgccg agcgtcatcc acagccgcac gttccagcgg ccttggtcac cgccttcgca cagccagttc gcgaggccga agtgatcggg gtggcagtgc gtgacgagca cgcgcagcac cggcagcccg tcgagatgcc gatcgaagat ctgttcccag tgcgtacgga tcgcctccga cgagatcccg cagtcgacga tcgtccagcc ggcctgcccg tcgatctcgt cgcgcagcag ccagagattg atgtgatcga gcgaaaacgg cagcggcatg cgcagccagc gcacgccggg tgcgacctcg aacgtgtcgc ccgcggcggg cagcgtgtcg gcgaagggat agtcgagttg gtgttccagt gcgttcatat ggtggtggtg gtggtgagcc at
Expressed Protein Sequence
MAHHHHHHMN ALEHQLDYPF ADTLPAAGDT FEVAPGVRWL RMPLPFSLDH INLWLLRDEI DGQAGWTIVD CGISSEAIRT HWEQIFDRHL DGLPVLRVLV THCHPDHFGL ANWLCEGGDQ GRWNVRLWMT LGEYMFGCLM AAGNGSNAGG AAAADHFARH GLTDPAALDK LRSRRSYYSD LVPAVPPRYR RLREGDAVKI GARTWRVVTG YGHSPEHCAL HSEADGVLIS GDMVLPRIST NVSVFDLEPE ANPLALYLES LGRYETMAPD TLVLPSHGKP FRGVRTRIAQ LRAHHDARLE EVRVACAEKP MSAAGIVPIM FRRRELDIHQ MTFALGEALA HLNLLWLAGE LVREHGDDGV IRYARA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAACG CACTGGAACA CCAACTCGAC TATCCCTTCG CCGACACGCT GCCCGCCGCG GGCGACACGT TCGAGGTCGC ACCCGGCGTG CGCTGGCTGC GCATGCCGCT GCCGTTTTCG CTCGATCACA TCAATCTCTG GCTGCTGCGC GACGAGATCG ACGGGCAGGC CGGCTGGACG ATCGTCGACT GCGGGATCTC GTCGGAGGCG ATCCGTACGC ACTGGGAACA GATCTTCGAT CGGCATCTCG ACGGGCTGCC GGTGCTGCGC GTGCTCGTCA CGCACTGCCA CCCCGATCAC TTCGGCCTCG CGAACTGGCT GTGCGAAGGC GGTGACCAAG GCCGCTGGAA CGTGCGGCTG TGGATGACGC TCGGCGAATA CATGTTCGGC TGCCTGATGG CGGCGGGCAA CGGCTCGAAC GCCGGCGGCG CGGCCGCGGC CGATCACTTC GCGCGCCACG GCTTGACCGA TCCGGCCGCG CTCGACAAGC TGCGCAGCCG CCGCAGCTAC TACTCGGATC TCGTGCCGGC CGTGCCGCCG CGCTACCGGC GCCTGCGCGA AGGCGACGCG GTGAAGATCG GCGCGCGCAC CTGGCGCGTC GTCACCGGCT ACGGCCATTC GCCTGAACAT TGCGCGCTGC ACAGCGAAGC GGACGGCGTG CTGATCTCCG GCGACATGGT GCTGCCGCGC ATCTCGACCA ACGTGTCCGT GTTCGATCTC GAACCGGAAG CGAACCCGCT CGCGCTGTAC CTCGAGTCGC TCGGCCGCTA CGAAACGATG GCGCCCGACA CGCTCGTGCT GCCGTCGCAC GGCAAGCCGT TCCGCGGCGT GCGCACGCGC ATCGCGCAAC TGCGCGCGCA CCACGACGCG CGGCTCGAGG AAGTGCGCGT CGCGTGCGCG GAGAAGCCGA TGAGTGCGGC CGGCATCGTG CCGATCATGT TCCGCCGCCG CGAGCTCGAC ATCCACCAGA TGACGTTCGC GCTCGGCGAG GCGCTCGCCC ACCTGAACCT GCTGTGGCTC GCGGGTGAGC TCGTGCGCGA GCACGGTGAC GACGGCGTGA TTCGCTACGC GAGGGCCTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT