SSGCID: Target Page

BuamA.12800.y

Transcriptional regulator, LysR family

CENTER ID:	BuamA.12800.y
ORGANISM:	Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuamA.12800.y.A1.GE31803	Full length( BuamA.12800.y )		1	301

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuamA.12800.y.A1.PW31710	Full length( BuamA.12800.y )		1	301

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|398577.6.peg.6063
RefSeq:	YP_001815608.1
UniProt:	B1Z5K9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDKFSALRAF VEVAEAGGFS SAGRRLDLAA SSVVRAVDAL EASLGTVLLN RTTRQVTLSD AGAVYYARAK QLLEALAEAD ALVADRGDEP SGPLRVSVPV AYGMHRIAPQ VGAFLARYPR LDLDLQLTDA RVDLVTSRID VAIRLGEAAP SAEVVARQLG TFRRYVVASP DYLDAHGTPS TPGELVDHGC LRFHFGGDQQ AWSFVDAHGA TKVLVTGRLK SNHSEVLRAA ALDGGGIALL PDWLVDADVR AGRLRRLFEQ YDVVPDAARS IVTALYLPNQ RGSKRVTAFI DFVEAQARTP C

NT Sequence

ATGGACAAAT TCTCCGCGCT GCGCGCGTTC GTTGAAGTAG CGGAAGCCGG CGGCTTCTCC AGCGCAGGGC GCCGCCTCGA TCTCGCCGCT TCGTCGGTGG TGCGCGCGGT AGACGCGCTC GAGGCATCGC TCGGCACCGT GCTGCTGAAC CGCACCACGC GACAGGTCAC GTTGTCCGAT GCGGGCGCCG TCTACTATGC GCGGGCGAAG CAGCTGCTCG AGGCGCTTGC GGAGGCCGAT GCACTCGTCG CCGATCGCGG CGACGAACCG TCAGGACCGC TGCGGGTGTC CGTGCCGGTC GCATACGGCA TGCACCGCAT CGCACCGCAG GTCGGCGCAT TCCTCGCGCG CTATCCCAGG CTCGATCTCG ATCTGCAACT CACCGACGCA CGCGTCGATC TCGTCACCAG CCGGATCGAC GTCGCGATCC GGCTCGGCGA AGCCGCGCCG TCCGCGGAAG TCGTCGCACG GCAGCTCGGC ACGTTCCGGC GGTATGTCGT GGCGAGCCCC GACTATCTCG ACGCGCACGG CACGCCATCG ACGCCGGGTG AGCTGGTCGA TCATGGCTGC CTGCGCTTTC ACTTCGGTGG CGATCAGCAG GCGTGGTCGT TCGTCGATGC GCACGGCGCG ACGAAGGTGC TCGTCACCGG GCGACTGAAA TCGAACCACA GCGAAGTATT GCGCGCCGCC GCGCTCGATG GCGGCGGCAT CGCGCTGCTG CCCGACTGGC TGGTCGATGC CGACGTCCGG GCGGGCCGCC TGCGCAGGCT GTTCGAACAG TACGACGTCG TGCCCGACGC GGCACGCTCG ATCGTCACCG CGCTCTACCT GCCGAATCAG CGGGGCTCGA AGCGCGTCAC CGCGTTCATC GATTTTGTCG AGGCGCAGGC TCGCACGCCG TGC

Details for BuamA.12800.y.A1.GE31803

HARVESTED ON:	3/8/2011
SEQUENCED ON:	3/14/2011
EXPECTED MW:	34kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMDKFSAL RAFVEVAEAG GFSSAGRRLD LAASSVVRAV DALEASLGTV LLNRTTRQVT LSDAGAVYYA RAKQLLEALA EADALVADRG DEPSGPLRVS VPVAYGMHRI APQVGAFLAR YPRLDLDLQL TDARVDLVTS RIDVAIRLGE AAPSAEVVAR QLGTFRRYVV ASPDYLDAHG TPSTPGELVD HGCLRFHFGG DQQAWSFVDA HGATKVLVTG RLKSNHSEVL RAAALDGGGI ALLPDWLVDA DVRAGRLRRL FEQYDVVPDA ARSIVTALYL PNQRGSKRVT AFIDFVEAQA RTPC

Validated NT Sequence

ttattagcac ggcgtgcgag cctgcgcctc gacaaaatcg atgaacgcgg tgacgcgctt cgagccccgc tgattcggca ggtagagcgc ggtgacgatc gagcgtgccg cgtcgggcac gacgtcgtac tgttcgaaca gcctgcgcag gcggcccgcc cggacgtcgg catcgaccag ccagtcgggc agcagcgcga tgccgccgcc atcgagcgcg gcggcgcgca atacttcgct gtggttcgat ttcagtcgcc cggtgacgag caccttcgtc gcgccgtgcg catcgacgaa cgaccacgcc tgctgatcgc caccgaagtg aaagcgcagg cagccatgat cgaccagctc acccggcgtc gatggcgtgc cgtgcgcgtc gagatagtcg gggctcgcca cgacataccg ccggaacgtg ccgagctgcc gtgcgacgac ttccgcggac ggcgcggctt cgccgagccg gatcgcgacg tcgatccggc tggtgacgag atcgacgcgt gcgtcggtga gttgcagatc gagatcgagc ctgggatagc gcgcgaggaa tgcgccgacc tgcggtgcga tgcggtgcat gccgtatgcg accggcacgg acacccgcag cggtcctgac ggttcgtcgc cgcgatcggc gacgagtgca tcggcctccg caagcgcctc gagcagctgc ttcgcccgcg catagtagac ggcgcccgca tcggacaacg tgacctgtcg cgtggtgcgg ttcagcagca cggtgccgag cgatgcctcg agcgcgtcta ccgcgcgcac caccgacgaa gcggcgagat cgaggcggcg ccctgcgctg gagaagccgc cggcttccgc tacttcaacg aacgcgcgca gcgcggagaa tttgtccatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgang gtg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMDKFSALRA FVEVAEAGGF SSAGRRLDLA ASSVVRAVDA LEASLGTVLL NRTTRQVTLS DAGAVYYARA KQLLEALAEA DALVADRGDE PSGPLRVSVP VAYGMHRIAP QVGAFLARYP RLDLDLQLTD ARVDLVTSRI DVAIRLGEAA PSAEVVARQL GTFRRYVVAS PDYLDAHGTP STPGELVDHG CLRFHFGGDQ QAWSFVDAHG ATKVLVTGRL KSNHSEVLRA AALDGGGIAL LPDWLVDADV RAGRLRRLFE QYDVVPDAAR SIVTALYLPN QRGSKRVTAF IDFVEAQART PC

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacaaattc tccgcgctgc gcgcgttcgt tgaagtagcg gaagccggcg gcttctccag cgcagggcgc cgcctcgatc tcgccgcttc gtcggtggtg cgcgcggtag acgcgctcga ggcatcgctc ggcaccgtgc tgctgaaccg caccacgcga caggtcacgt tgtccgatgc gggcgccgtc tactatgcgc gggcgaagca gctgctcgag gcgcttgcgg aggccgatgc actcgtcgcc gatcgcggcg acgaaccgtc aggaccgctg cgggtgtccg tgccggtcgc atacggcatg caccgcatcg caccgcaggt cggcgcattc ctcgcgcgct atcccaggct cgatctcgat ctgcaactca ccgacgcacg cgtcgatctc gtcaccagcc ggatcgacgt cgcgatccgg ctcggcgaag ccgcgccgtc cgcggaagtc gtcgcacggc agctcggcac gttccggcgg tatgtcgtgg cgagccccga ctatctcgac gcgcacggca cgccatcgac gccgggtgag ctggtcgatc atggctgcct gcgctttcac ttcggtggcg atcagcaggc gtggtcgttc gtcgatgcgc acggcgcgac gaaggtgctc gtcaccgggc gactgaaatc gaaccacagc gaagtattgc gcgccgccgc gctcgatggc ggcggcatcg cgctgctgcc cgactggctg gtcgatgccg acgtccgggc gggccgcctg cgcaggctgt tcgaacagta cgacgtcgtg cccgacgcgg cacgctcgat cgtcaccgcg ctctacctgc cgaatcagcg gggctcgaag cgcgtcaccg cgttcatcga ttttgtcgag gcgcaggctc gcacgccgtg ctaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa

Details for BuamA.12800.y.A1.PW31710

PURIFICATION DATe:	7/28/2011
CONCENTRATION:	21.84mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	9
VIAL VOLUME:	100µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGACAAATTC TCCGCGCTGC GCGCGTTCGT TGAAGTAGCG GAAGCCGGCG GCTTCTCCAG CGCAGGGCGC CGCCTCGATC TCGCCGCTTC GTCGGTGGTG CGCGCGGTAG ACGCGCTCGA GGCATCGCTC GGCACCGTGC TGCTGAACCG CACCACGCGA CAGGTCACGT TGTCCGATGC GGGCGCCGTC TACTATGCGC GGGCGAAGCA GCTGCTCGAG GCGCTTGCGG AGGCCGATGC ACTCGTCGCC GATCGCGGCG ACGAACCGTC AGGACCGCTG CGGGTGTCCG TGCCGGTCGC ATACGGCATG CACCGCATCG CACCGCAGGT CGGCGCATTC CTCGCGCGCT ATCCCAGGCT CGATCTCGAT CTGCAACTCA CCGACGCACG CGTCGATCTC GTCACCAGCC GGATCGACGT CGCGATCCGG CTCGGCGAAG CCGCGCCGTC CGCGGAAGTC GTCGCACGGC AGCTCGGCAC GTTCCGGCGG TATGTCGTGG CGAGCCCCGA CTATCTCGAC GCGCACGGCA CGCCATCGAC GCCGGGTGAG CTGGTCGATC ATGGCTGCCT GCGCTTTCAC TTCGGTGGCG ATCAGCAGGC GTGGTCGTTC GTCGATGCGC ACGGCGCGAC GAAGGTGCTC GTCACCGGGC GACTGAAATC GAACCACAGC GAAGTATTGC GCGCCGCCGC GCTCGATGGC GGCGGCATCG CGCTGCTGCC CGACTGGCTG GTCGATGCCG ACGTCCGGGC GGGCCGCCTG CGCAGGCTGT TCGAACAGTA CGACGTCGTG CCCGACGCGG CACGCTCGAT CGTCACCGCG CTCTACCTGC CGAATCAGCG GGGCTCGAAG CGCGTCACCG CGTTCATCGA TTTTGTCGAG GCGCAGGCTC GCACGCCGTG CTAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA