BuamA.12800.x

Transcriptional regulator, LysR family

CENTER ID: BuamA.12800.x
ORGANISM: Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.12800.x.A1.GE31802 Full length( BuamA.12800.x ) 1 345
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|398577.6.peg.6010
RefSeq: YP_001815553.1
UniProt: B1Z5F4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNQSDTAEHG ARRDLAGRAD ALSASFGASY AGILAFVAVA SEGSFTKAAE LLGVGRPAVS RNVQKLEAQL STRLFQRTTR TTELTCEGRR FFENCHQGVA QIVEAMNDML ELRQGPPRGL IRISSAVGFG RKIVAPLLEK FAQAYPDIII DLLLDDRPID LVSERIDVSF RNGRIEDSSI IARQLIPMQM ALCAAPSYVA RHGLPQRIDD LDQHECINFR FASGRVFEWE FNVDGRMQKY LPTSRLTFND ADLVLRAVLN GAGIAQMAGY QIRDHLASKE LVVALAKHVP EDRGHYICYL SRQHLPTRIR IFVDFMTEQI RALDLNCMTR FNPDMQAVPS AGLAA
NT Sequence
ATGAATCAAT CGGATACAGC GGAGCACGGC GCTCGTCGAG ACCTGGCCGG TCGTGCCGAT GCACTTTCGG CGAGCTTCGG TGCGAGCTAC GCGGGCATCC TTGCTTTTGT CGCGGTGGCG AGCGAGGGCA GTTTTACGAA AGCCGCCGAG TTGCTCGGCG TCGGACGGCC GGCCGTGAGC CGCAACGTGC AGAAGCTGGA GGCGCAGTTG AGCACGCGGC TGTTCCAGCG CACCACGCGC ACGACCGAAC TGACGTGCGA AGGCCGGCGA TTCTTCGAGA ACTGCCATCA GGGCGTGGCA CAGATCGTGG AAGCCATGAA CGACATGCTG GAGTTACGGC AGGGGCCGCC ACGCGGCCTC ATTCGCATCA GCTCGGCCGT GGGGTTCGGC AGGAAGATCG TCGCGCCGCT GCTGGAAAAA TTCGCGCAGG CCTACCCGGA CATCATCATC GATCTGCTGC TCGATGACCG GCCGATCGAC CTGGTGTCGG AGCGGATCGA CGTGTCGTTC CGCAACGGGC GCATCGAGGA TTCGAGCATC ATCGCCCGGC AACTGATTCC GATGCAGATG GCGCTCTGCG CGGCGCCGTC GTATGTCGCA CGGCATGGCC TCCCGCAGCG CATCGACGAC CTCGACCAGC ACGAGTGCAT CAATTTCCGC TTTGCGAGCG GTCGCGTGTT CGAATGGGAG TTCAATGTCG ACGGCCGCAT GCAAAAGTAC CTGCCGACGT CGCGCCTCAC GTTCAACGAC GCGGATCTGG TGTTGCGCGC GGTGCTGAAC GGAGCGGGCA TCGCGCAGAT GGCGGGCTAC CAGATCCGCG ACCATCTCGC GTCGAAGGAG CTCGTCGTCG CACTGGCGAA GCATGTGCCG GAGGATCGCG GCCACTACAT CTGCTATCTG AGCCGGCAGC ATCTGCCGAC GCGCATCCGG ATCTTCGTCG ACTTCATGAC CGAGCAGATT CGCGCGCTCG ATCTGAACTG CATGACCCGG TTCAATCCTG ACATGCAGGC GGTTCCATCG GCGGGGCTGG CGGCT
Details for BuamA.12800.x.A1.GE31802
HARVESTED ON: 3/8/2011
SEQUENCED ON: 3/14/2011
EXPECTED MW: 40kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 91
PERCENT COVERAGE: 89
Validated AA Sequence
MHFRRASVRA TRASLLLSRW RARAVLRKPP SCSASDGPAV SRNVQKLEAQ LSTRLFQRTT RTTELTCEGR RFFENCHQGV AQIVEAMNDM LELRQGPPRG LIRISSAVGF GRKIVAPLLE KFAQAYPDII IDLLLDDRPI DLVSERIDVS FRNGRIEDSS IIARQLIPMQ MALCAAPSYV ARHGLPQRID DLDQHECINF RFASGRVFEW EFNVDGRMQK YLPTSRLTFN DADLVLRAVL NGAGIAQMAG YQIRDHLASK ELVVALAKHV PEDRGHYICY LSRQHLPTRI RIFVDFMTEQ IRALDLNCMT RFNPDMQAVP SAGLAA
Validated NT Sequence
ttattaagcc gccagccccg ccgatggaac cgcctgcatg tcaggattga accgggtcat gcagttcaga tcgagcgcgc gaatctgctc ggtcatgaag tcgacgaaga tccggatgcg cgtcggcaga tgctgccggc tcagatagca gatgtagtgg ccgcgatcct ccggcacatg cttcgccagt gcgacgacga gctccttcga cgcgagatgg tcgcggatct ggtagcccgc catctgcgcg atgcccgctc cgttcagcac cgcgcgcaac accagatccg cgtcgttgaa cgtgaggcgc gacgtcggca ggtacttttg catgcggccg tcgacattga actcccattc gaacacgcga ccgctcgcaa agcggaaatt gatgcactcg tgctggtcga ggtcgtcgat gcgctgcggg aggccatgcc gtgcgacata cgacggcgcc gcgcagagcg ccatctgcat cggaatcagt tgccgggcga tgatgctcga atcctcgatg cgcccgttgc ggaacgacac gtcgatccgc tccgacacca ggtcgatcgg ccggtcatcg agcagcagat cgatgatgat gtccgggtag gcctgcgcga atttttccag cagcggcgcg acgatcttcc tgccgaaccc cacggccgag ctgatgcgaa tgaggccgcg tggcggcccc tgccgtaact ccagcatgtc gttcatggct tccacgatct gtgccacgcc ctgatggcag ttctcgaaga atcgccggcc ttcgcacgtc agttcggtcg tgcgcgtggt gcgctggaac agccgcgtgc tcaactgcgc ctccagcttc tgcacgttgc ggctcacggc cgggccgtcc gacgccgagc aactcggcgg ctttcgtaaa actgccctcg ctcgccaccg cgacaaaagc aaggatgccc gcgtagctcg caccgaagct cgccgaaagt gcatcggcac gaccggccag gtctcgacga gcgccgtgct ccgctgtatc cgattgattc atcgaaccag gaccctgggt ntgagct
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNQSDTAEH GARRDLAGRA DALSASFGAS YAGILAFVAV ASEGSFTKAA ELLGVGRPAV SRNVQKLEAQ LSTRLFQRTT RTTELTCEGR RFFENCHQGV AQIVEAMNDM LELRQGPPRG LIRISSAVGF GRKIVAPLLE KFAQAYPDII IDLLLDDRPI DLVSERIDVS FRNGRIEDSS IIARQLIPMQ MALCAAPSYV ARHGLPQRID DLDQHECINF RFASGRVFEW EFNVDGRMQK YLPTSRLTFN DADLVLRAVL NGAGIAQMAG YQIRDHLASK ELVVALAKHV PEDRGHYICY LSRQHLPTRI RIFVDFMTEQ IRALDLNCMT RFNPDMQAVP SAGLAA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaatcaatcg gatacagcgg agcacggcgc tcgtcgagac ctggccggtc gtgccgatgc actttcggcg agcttcggtg cgagctacgc gggcatcctt gcttttgtcg cggtggcgag cgagggcagt tttacgaaag ccgccgagtt gctcggcgtc ggacggccgg ccgtgagccg caacgtgcag aagctggagg cgcagttgag cacgcggctg ttccagcgca ccacgcgcac gaccgaactg acgtgcgaag gccggcgatt cttcgagaac tgccatcagg gcgtggcaca gatcgtggaa gccatgaacg acatgctgga gttacggcag gggccgccac gcggcctcat tcgcatcagc tcggccgtgg ggttcggcag gaagatcgtc gcgccgctgc tggaaaaatt cgcgcaggcc tacccggaca tcatcatcga tctgctgctc gatgaccggc cgatcgacct ggtgtcggag cggatcgacg tgtcgttccg caacgggcgc atcgaggatt cgagcatcat cgcccggcaa ctgattccga tgcagatggc gctctgcgcg gcgccgtcgt atgtcgcacg gcatggcctc ccgcagcgca tcgacgacct cgaccagcac gagtgcatca atttccgctt tgcgagcggt cgcgtgttcg aatgggagtt caatgtcgac ggccgcatgc aaaagtacct gccgacgtcg cgcctcacgt tcaacgacgc ggatctggtg ttgcgcgcgg tgctgaacgg agcgggcatc gcgcagatgg cgggctacca gatccgcgac catctcgcgt cgaaggagct cgtcgtcgca ctggcgaagc atgtgccgga ggatcgcggc cactacatct gctatctgag ccggcagcat ctgccgacgc gcatccggat cttcgtcgac ttcatgaccg agcagattcg cgcgctcgat ctgaactgca tgacccggtt caatcctgac atgcaggcgg ttccatcggc ggggctggcg gcttaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa