BuamA.12800.w

Transcriptional regulator, LysR family

CENTER ID: BuamA.12800.w
ORGANISM: Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.12800.w.A1.GE31801 Full length( BuamA.12800.w ) 1 305
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.12800.w.A1.PW31708 Full length( BuamA.12800.w ) 1 305

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|398577.6.peg.6416
RefSeq: YP_001815940.1
UniProt: B1Z3S4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDRFDAMRAF VRVVEAGSFT KAAETLHMSK TTVTQLVQQL EARLRVRLLN RTTRRINVTP DGIAYYERVT RLLADMDDAE TSLSSASAVP RGRLRVDVPS PFARLILIPA LPAFLARYPD IQLDMGVSDR TVDIIGENVD CVVRGGEPND QSMVARRVAD LSLGIVASPG YLALASTPSH PAELENSHHR IVGFSWGRTG KLYPLVMHRG DETVAVQGRY ALAVDDGNAY LAAGVAGLGI MWLPHYMAKA HLERGELVTL FDDWQLDPMP LYIAYPPNRH VSAKLRAFID WVVELMNAHA PVEPQ
NT Sequence
ATGGATCGCT TCGATGCGAT GCGCGCTTTC GTCCGGGTCG TCGAGGCGGG CAGCTTCACG AAGGCCGCCG AGACGCTGCA TATGAGCAAG ACCACAGTCA CGCAGCTGGT CCAGCAGCTC GAGGCGCGCT TGCGGGTCCG ACTGCTGAAC CGGACGACGC GCAGGATCAA CGTCACGCCG GACGGCATCG CGTACTACGA GCGCGTGACG CGGCTGCTCG CCGATATGGA CGACGCCGAA ACGAGCCTGT CGAGCGCATC GGCGGTGCCA CGAGGCCGGC TGCGGGTGGA CGTGCCGAGT CCGTTCGCGC GCCTGATCCT GATCCCCGCG CTGCCGGCCT TCCTCGCCCG CTATCCGGAC ATTCAGCTCG ACATGGGCGT GAGCGACCGC ACCGTCGACA TCATCGGCGA GAACGTGGAT TGCGTCGTGC GCGGCGGCGA GCCGAACGAT CAGTCGATGG TCGCCCGGCG CGTGGCGGAC CTGTCGCTCG GCATCGTTGC GTCGCCCGGC TATCTTGCGC TCGCGAGCAC GCCTTCGCAT CCCGCGGAAC TCGAAAACTC GCATCACCGC ATCGTCGGGT TCTCATGGGG ACGCACGGGC AAACTCTATC CGCTCGTCAT GCATCGCGGC GACGAGACCG TCGCGGTGCA AGGCCGTTAC GCGCTCGCCG TCGATGACGG CAATGCCTAT CTCGCGGCCG GCGTGGCCGG CCTCGGCATC ATGTGGCTGC CGCACTATAT GGCGAAAGCG CATCTGGAGC GCGGCGAACT CGTCACGCTG TTCGACGACT GGCAGCTCGA TCCGATGCCG CTATACATCG CGTATCCGCC GAACCGCCAC GTCAGTGCCA AACTGCGTGC GTTCATCGAC TGGGTCGTCG AGCTGATGAA CGCGCATGCT CCCGTGGAAC CGCAA
Details for BuamA.12800.w.A1.GE31801
HARVESTED ON: 3/8/2011
SEQUENCED ON: 3/14/2011
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Validated AA Sequence
MGTLEAQTQG PGSMDRFDAM RAFVRVVEAG SFTKAAETLH MSKTTVTQLV QQLEARLRVR LLNRTTRRIN VTPDGIAYYE RVTRLLADMD DAETSLSSAS AVPRGRLRVD VPSPFARLIL IPALPAFLAR YPDIQLDMGV SDRTVDIIGE NVDCVVRGGE PNDQSMVARR VADLSLGIVA SPGYLALAST PSHPAELENS HHRIVGFSWG RTGKLYPLVM HRGDETVAVQ GRYALAVDDG NAYLAAGVAG LGIMWLPHYM AKAHLERGEL VTLFDDWQLD PMPLYIAYPP NRHVSAKLRA FIDWVVELMN AHAP
Validated NT Sequence
acgggagcat gcgcgttcat cagctcgacg acccagtcga tgaacgcacg cagtttggca ctgacgtggc ggttcggcgg atacgcgatg tatagcggca tcggatcgag ctgccagtcg tcgaacagcg tgacgagttc gccgcgctcc agatgcgctt tcgccatata gtgcggcagc cacatgatgc cgaggccggc cacgccggcc gcgagatagg cattgccgtc atcgacggcg agcgcgtaac ggccttgcac cgcgacggtc tcgtcgccgc gatgcatgac gagcggatag agtttgcccg tgcgtcccca tgagaacccg acgatgcggt gatgcgagtt ttcgagttcc gcgggatgcg aaggcgtgct cgcgagcgca agatagccgg gcgacgcaac gatgccgagc gacaggtccg ccacgcgccg ggcgaccatc gactgatcgt tcggctcgcc gccgcgcacg acgcaatcca cgttctcgcc gatgatgtcg acggtgcggt cgctcacgcc catgtcgagc tgaatgtccg gatagcgggc gaggaaggcc ggcagcgcgg ggatcaggat caggcgcgcg aacggactcg gcacgtccac ccgcagccgg cctcgtggca ccgccgatgc gctcgacagg ctcgtttcgg cgtcgtccat atcggcgagc agccgcgtca cgcgctcgta gtacgcgatg ccgtccggcg tgacgttgat cctgcgcgtc gtccggttca gcagtcggac ccgcaagcgc gcctcgagct gctggaccag ctgcgtgact gtggtcttgc tcatatgcag cgtctcggcg gccttcgtga agctgcccgc ctcgacgacc cggacgaaag cgcgcatcgc atcgaagcga tccatcgaac caggaccctg ggtctgagct tccagggtac ccata
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDRFDAMRA FVRVVEAGSF TKAAETLHMS KTTVTQLVQQ LEARLRVRLL NRTTRRINVT PDGIAYYERV TRLLADMDDA ETSLSSASAV PRGRLRVDVP SPFARLILIP ALPAFLARYP DIQLDMGVSD RTVDIIGENV DCVVRGGEPN DQSMVARRVA DLSLGIVASP GYLALASTPS HPAELENSHH RIVGFSWGRT GKLYPLVMHR GDETVAVQGR YALAVDDGNA YLAAGVAGLG IMWLPHYMAK AHLERGELVT LFDDWQLDPM PLYIAYPPNR HVSAKLRAFI DWVVELMNAH APVEPQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggatcgcttc gatgcgatgc gcgctttcgt ccgggtcgtc gaggcgggca gcttcacgaa ggccgccgag acgctgcata tgagcaagac cacagtcacg cagctggtcc agcagctcga ggcgcgcttg cgggtccgac tgctgaaccg gacgacgcgc aggatcaacg tcacgccgga cggcatcgcg tactacgagc gcgtgacgcg gctgctcgcc gatatggacg acgccgaaac gagcctgtcg agcgcatcgg cggtgccacg aggccggctg cgggtggacg tgccgagtcc gttcgcgcgc ctgatcctga tccccgcgct gccggccttc ctcgcccgct atccggacat tcagctcgac atgggcgtga gcgaccgcac cgtcgacatc atcggcgaga acgtggattg cgtcgtgcgc ggcggcgagc cgaacgatca gtcgatggtc gcccggcgcg tggcggacct gtcgctcggc atcgttgcgt cgcccggcta tcttgcgctc gcgagcacgc cttcgcatcc cgcggaactc gaaaactcgc atcaccgcat cgtcgggttc tcatggggac gcacgggcaa actctatccg ctcgtcatgc atcgcggcga cgagaccgtc gcggtgcaag gccgttacgc gctcgccgtc gatgacggca atgcctatct cgcggccggc gtggccggcc tcggcatcat gtggctgccg cactatatgg cgaaagcgca tctggagcgc ggcgaactcg tcacgctgtt cgacgactgg cagctcgatc cgatgccgct atacatcgcg tatccgccga accgccacgt cagtgccaaa ctgcgtgcgt tcatcgactg ggtcgtcgag ctgatgaacg cgcatgctcc cgtggaaccg caataaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for BuamA.12800.w.A1.PW31708
PURIFICATION DATe: 7/15/2011
CONCENTRATION: 25.5mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMDRFDAM RAFVRVVEAG SFTKAAETLH MSKTTVTQLV QQLEARLRVR LLNRTTRRIN VTPDGIAYYE RVTRLLADMD DAETSLSSAS AVPRGRLRVD VPSPFARLIL IPALPAFLAR YPDIQLDMGV SDRTVDIIGE NVDCVVRGGE PNDQSMVARR VADLSLGIVA SPGYLALAST PSHPAELENS HHRIVGFSWG RTGKLYPLVM HRGDETVAVQ GRYALAVDDG NAYLAAGVAG LGIMWLPHYM AKAHLERGEL VTLFDDWQLD PMPLYIAYPP NRHVSAKLRA FIDWVVELMN AHAP
Validated NT Sequence
acgggagcat gcgcgttcat cagctcgacg acccagtcga tgaacgcacg cagtttggca ctgacgtggc ggttcggcgg atacgcgatg tatagcggca tcggatcgag ctgccagtcg tcgaacagcg tgacgagttc gccgcgctcc agatgcgctt tcgccatata gtgcggcagc cacatgatgc cgaggccggc cacgccggcc gcgagatagg cattgccgtc atcgacggcg agcgcgtaac ggccttgcac cgcgacggtc tcgtcgccgc gatgcatgac gagcggatag agtttgcccg tgcgtcccca tgagaacccg acgatgcggt gatgcgagtt ttcgagttcc gcgggatgcg aaggcgtgct cgcgagcgca agatagccgg gcgacgcaac gatgccgagc gacaggtccg ccacgcgccg ggcgaccatc gactgatcgt tcggctcgcc gccgcgcacg acgcaatcca cgttctcgcc gatgatgtcg acggtgcggt cgctcacgcc catgtcgagc tgaatgtccg gatagcgggc gaggaaggcc ggcagcgcgg ggatcaggat caggcgcgcg aacggactcg gcacgtccac ccgcagccgg cctcgtggca ccgccgatgc gctcgacagg ctcgtttcgg cgtcgtccat atcggcgagc agccgcgtca cgcgctcgta gtacgcgatg ccgtccggcg tgacgttgat cctgcgcgtc gtccggttca gcagtcggac ccgcaagcgc gcctcgagct gctggaccag ctgcgtgact gtggtcttgc tcatatgcag cgtctcggcg gccttcgtga agctgcccgc ctcgacgacc cggacgaaag cgcgcatcgc atcgaagcga tccatcgaac caggaccctg ggtctgagct tccagggtac ccata
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDRFDAMRA FVRVVEAGSF TKAAETLHMS KTTVTQLVQQ LEARLRVRLL NRTTRRINVT PDGIAYYERV TRLLADMDDA ETSLSSASAV PRGRLRVDVP SPFARLILIP ALPAFLARYP DIQLDMGVSD RTVDIIGENV DCVVRGGEPN DQSMVARRVA DLSLGIVASP GYLALASTPS HPAELENSHH RIVGFSWGRT GKLYPLVMHR GDETVAVQGR YALAVDDGNA YLAAGVAGLG IMWLPHYMAK AHLERGELVT LFDDWQLDPM PLYIAYPPNR HVSAKLRAFI DWVVELMNAH APVEPQ
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGATCGCTTC GATGCGATGC GCGCTTTCGT CCGGGTCGTC GAGGCGGGCA GCTTCACGAA GGCCGCCGAG ACGCTGCATA TGAGCAAGAC CACAGTCACG CAGCTGGTCC AGCAGCTCGA GGCGCGCTTG CGGGTCCGAC TGCTGAACCG GACGACGCGC AGGATCAACG TCACGCCGGA CGGCATCGCG TACTACGAGC GCGTGACGCG GCTGCTCGCC GATATGGACG ACGCCGAAAC GAGCCTGTCG AGCGCATCGG CGGTGCCACG AGGCCGGCTG CGGGTGGACG TGCCGAGTCC GTTCGCGCGC CTGATCCTGA TCCCCGCGCT GCCGGCCTTC CTCGCCCGCT ATCCGGACAT TCAGCTCGAC ATGGGCGTGA GCGACCGCAC CGTCGACATC ATCGGCGAGA ACGTGGATTG CGTCGTGCGC GGCGGCGAGC CGAACGATCA GTCGATGGTC GCCCGGCGCG TGGCGGACCT GTCGCTCGGC ATCGTTGCGT CGCCCGGCTA TCTTGCGCTC GCGAGCACGC CTTCGCATCC CGCGGAACTC GAAAACTCGC ATCACCGCAT CGTCGGGTTC TCATGGGGAC GCACGGGCAA ACTCTATCCG CTCGTCATGC ATCGCGGCGA CGAGACCGTC GCGGTGCAAG GCCGTTACGC GCTCGCCGTC GATGACGGCA ATGCCTATCT CGCGGCCGGC GTGGCCGGCC TCGGCATCAT GTGGCTGCCG CACTATATGG CGAAAGCGCA TCTGGAGCGC GGCGAACTCG TCACGCTGTT CGACGACTGG CAGCTCGATC CGATGCCGCT ATACATCGCG TATCCGCCGA ACCGCCACGT CAGTGCCAAA CTGCGTGCGT TCATCGACTG GGTCGTCGAG CTGATGAACG CGCATGCTCC CGTGGAACCG CAATAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA