SSGCID: Target Page

BuamA.12800.v

Transcriptional regulator, LysR family

CENTER ID:	BuamA.12800.v
ORGANISM:	Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuamA.12800.v.A2.GE31800	Mature protein( BuamA.12800.v )		27	345

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|398577.6.peg.6334
RefSeq:	YP_001815864.1
UniProt:	B1Z3J8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRSSSEKLSG SISVFAAVVD MGTFAAASEV IGMTPPGVSR AIARLEKKLN IRLFNRTTRS VSLTEEGRRF YEQVMPHLAG LEEAAAAAAG SASAVRGKLR VNLDPVVYRT ILGPQLDSFM DAHPELEIEL IARDSLGDLV MDGFDIAVRF GEPRASTLIA RKLLDTGIVT VASPAYIARW GRPTKPEDLE SRSHRCIEFR NPETGKPFAW EFHRRRKRIV VDTRGRLTVN DPGALLNACL AGSGIAQMLL LGAEPLIANG QLINLFPEWA DERFPLYAYY PSRHHLPAKT RAFLDFVVEL MATMSASARA KHPRSRHQSH LKAAARHIAP YRSGRCPVRR RTAKG

NT Sequence

ATGAGATCCA GTAGCGAGAA GCTGTCCGGC AGCATCAGCG TGTTCGCCGC CGTGGTGGAC ATGGGGACGT TCGCGGCCGC ATCGGAAGTC ATAGGAATGA CACCACCGGG CGTTAGTCGC GCAATCGCGC GACTGGAAAA AAAGCTCAAC ATCCGGCTCT TCAACCGAAC GACACGCTCC GTCTCGCTCA CTGAAGAGGG CCGCCGCTTT TACGAGCAGG TCATGCCCCA TCTCGCCGGG CTGGAGGAAG CAGCCGCGGC TGCGGCAGGT AGCGCCTCCG CTGTGCGTGG AAAGCTGAGA GTCAATCTTG ATCCTGTCGT CTACCGGACC ATTCTTGGGC CGCAACTCGA TTCGTTCATG GACGCGCACC CGGAACTCGA AATCGAGCTC ATCGCACGAG ACAGTCTTGG CGACCTCGTC ATGGATGGCT TCGACATCGC CGTGCGATTC GGCGAGCCCC GAGCCTCTAC GCTGATCGCG AGAAAGTTGC TCGATACCGG CATTGTTACG GTCGCATCGC CCGCGTATAT CGCTCGCTGG GGACGGCCGA CAAAACCCGA GGACCTGGAA AGCCGAAGCC ATCGATGCAT TGAATTTCGC AACCCGGAGA CCGGCAAGCC GTTCGCGTGG GAATTCCATC GCCGGCGAAA GCGCATTGTG GTCGACACCC GGGGACGCCT CACGGTCAAC GATCCGGGTG CCCTGCTCAA CGCATGCCTC GCCGGTTCCG GAATTGCGCA GATGTTGCTG CTCGGTGCCG AGCCCCTGAT CGCGAACGGC CAATTGATCA ATCTGTTTCC CGAATGGGCA GACGAGCGAT TTCCGTTATA CGCGTACTAC CCGTCCCGGC ATCACCTGCC GGCAAAGACG CGGGCGTTTC TTGACTTCGT GGTTGAGCTG ATGGCAACCA TGAGCGCGTC GGCACGCGCC AAGCACCCGC GATCTCGCCA TCAGTCGCAT CTCAAAGCAG CAGCGAGACA CATAGCGCCA TACCGGTCAG GTAGGTGCCC AGTGCGCCGA CGAACCGCAA AGGGT

Details for BuamA.12800.v.A2.GE31800

HARVESTED ON:	3/8/2011
SEQUENCED ON:	3/14/2011
EXPECTED MW:	37kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMASEVIG MTPPGVSRAI ARLEKKLNIR LFNRTTRSVS LTEEGRRFYE QVMPHLAGLE EAAAAAAGSA SAVRGKLRVN LDPVVYRTIL GPQLDSFMDA HPELEIELIA RDSLGDLVMD GFDIAVRFGE PRASTLIARK LLDTGIVTVA SPAYIARWGR PTKPEDLESR SHRCIEFRNP ETGKPFAWEF HRRRKRIVVD TRGRLTVNDP GALLNACLAG SGIAQMLLLG AEPLIANGQL INLFPEWADE RFPLYAYYPS RHHLPAKTRA FLDFVVELMA TMSASARAKH PRSRHQSHLK AAARHIAPYR SGRCPVRRRT A

Validated NT Sequence

ttgcggttcg tcggcgcact gggcacctac ctgaccggta tggcgctatg tgtctcgctg ctgctttgag atgcgactga tggcgagatc gcgggtgctt ggcgcgtgcc gacgcgctca tggttgccat cagctcaacc acgaagtcaa gaaacgcccg cgtctttgcc ggcaggtgat gccgggacgg gtagtacgcg tataacggaa atcgctcgtc tgcccattcg ggaaacagat tgatcaattg gccgttcgcg atcaggggct cggcaccgag cagcaacatc tgcgcaattc cggaaccggc gaggcatgcg ttgagcaggg cacccggatc gttgaccgtg aggcgtcccc gggtgtcgac cacaatgcgc tttcgccggc gatggaattc ccacgcgaac ggcttgccgg tctccgggtt gcgaaattca atgcatcgat ggcttcggct ttccaggtcc tcgggttttg tcggccgtcc ccagcgagcg atatacgcgg gcgatgcgac cgtaacaatg ccggtatcga gcaactttct cgcgatcagc gtagaggctc ggggctcgcc gaatcgcacg gcgatgtcga agccatccat gacgaggtcg ccaagactgt ctcgtgcgat gagctcgatt tcgagttccg ggtgcgcgtc catgaacgaa tcgagttgcg gcccaagaat ggtccggtag acgacaggat caagattgac tctcagcttt ccacgcacag cggaggcgct acctgccgca gccgcggctg cttcctccag cccggcgaga tggggcatga cctgctcgta aaagcggcgg ccctcttcag tgagcgagac ggagcgtgtc gttcggttga agagccggat gttgagcttt ttttccagtc gcgcgattgc gcgactaacg cccggtggtg tcattcctat gacttccgat gccatcgaac caggaccctg ggtctgagct tccagggtac ccatatga

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMASEVIGMT PPGVSRAIAR LEKKLNIRLF NRTTRSVSLT EEGRRFYEQV MPHLAGLEEA AAAAAGSASA VRGKLRVNLD PVVYRTILGP QLDSFMDAHP ELEIELIARD SLGDLVMDGF DIAVRFGEPR ASTLIARKLL DTGIVTVASP AYIARWGRPT KPEDLESRSH RCIEFRNPET GKPFAWEFHR RRKRIVVDTR GRLTVNDPGA LLNACLAGSG IAQMLLLGAE PLIANGQLIN LFPEWADERF PLYAYYPSRH HLPAKTRAFL DFVVELMATM SASARAKHPR SRHQSHLKAA ARHIAPYRSG RCPVRRRTAK G

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcatcggaa gtcataggaa tgacaccacc gggcgttagt cgcgcaatcg cgcgactgga aaaaaagctc aacatccggc tcttcaaccg aacgacacgc tccgtctcgc tcactgaaga gggccgccgc ttttacgagc aggtcatgcc ccatctcgcc gggctggagg aagcagccgc ggctgcggca ggtagcgcct ccgctgtgcg tggaaagctg agagtcaatc ttgatcctgt cgtctaccgg accattcttg ggccgcaact cgattcgttc atggacgcgc acccggaact cgaaatcgag ctcatcgcac gagacagtct tggcgacctc gtcatggatg gcttcgacat cgccgtgcga ttcggcgagc cccgagcctc tacgctgatc gcgagaaagt tgctcgatac cggcattgtt acggtcgcat cgcccgcgta tatcgctcgc tggggacggc cgacaaaacc cgaggacctg gaaagccgaa gccatcgatg cattgaattt cgcaacccgg agaccggcaa gccgttcgcg tgggaattcc atcgccggcg aaagcgcatt gtggtcgaca cccggggacg cctcacggtc aacgatccgg gtgccctgct caacgcatgc ctcgccggtt ccggaattgc gcagatgttg ctgctcggtg ccgagcccct gatcgcgaac ggccaattga tcaatctgtt tcccgaatgg gcagacgagc gatttccgtt atacgcgtac tacccgtccc ggcatcacct gccggcaaag acgcgggcgt ttcttgactt cgtggttgag ctgatggcaa ccatgagcgc gtcggcacgc gccaagcacc cgcgatctcg ccatcagtcg catctcaaag cagcagcgag acacatagcg ccataccggt caggtaggtg cccagtgcgc cgacgaaccg caaagggtta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a