BuamA.12800.u

Transcriptional regulator, LysR family

CENTER ID: BuamA.12800.u
ORGANISM: Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.12800.u.A1.GE31799 Full length( BuamA.12800.u ) 1 315
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|398577.6.peg.4354
RefSeq: YP_001810894.1
UniProt: B1Z398

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDTLQNMRVF TRVVETGSFT AAAQSLNSTT GAMSRAVSEL EARLRTRLMN RSTRRLALTS AGESYLRRCR QILADVDRAE EEASCAHERP AGVLRMHSFA SVGHHYVMPA LTRYHTQYPD VSIELSLSQR MPDLFDGTSD MAVVTASSLP DSELVSHLLG STFSILCASP EYVKRHGAPA RPQDLGAHAC LTLCTPAFPT HEWVLEGAEG VEQIHVAGPV QTNTAESLAL AIRDGIGIGM LPLYSAIDAL RDGTLVRVLP EHILQKMNVY ALYPSRRFVD AKVRTWVEML RAQVPGMIAR DVEMLNAIDR EPQAA
NT Sequence
ATGGATACCC TACAAAACAT GCGGGTGTTT ACGCGCGTCG TCGAGACGGG CAGTTTCACC GCGGCCGCGC AATCGCTGAA TTCGACGACG GGCGCGATGT CGCGCGCGGT GTCGGAGCTC GAGGCGCGCC TGCGCACCCG TCTGATGAAT CGCTCGACAC GCCGTCTCGC GCTGACGTCG GCCGGCGAAA GCTATCTGCG GCGCTGCCGG CAGATCCTCG CCGACGTCGA TCGCGCCGAG GAAGAAGCAA GCTGCGCGCA CGAGCGGCCA GCCGGCGTGC TGCGCATGCA CAGCTTCGCG AGCGTCGGCC ATCACTACGT GATGCCCGCG CTGACGCGCT ATCACACGCA GTATCCGGAC GTGTCGATCG AGCTGTCGCT GTCGCAGCGC ATGCCCGACC TGTTCGACGG CACGAGCGAC ATGGCGGTCG TGACCGCGTC GTCGCTGCCC GATTCGGAAC TCGTGTCGCA CCTGCTGGGC TCGACCTTCA GCATCCTGTG CGCGTCGCCC GAGTACGTGA AGCGCCACGG CGCGCCCGCA CGCCCGCAGG ATCTCGGCGC GCACGCATGC CTGACGCTCT GCACGCCGGC CTTCCCGACT CACGAATGGG TGCTCGAAGG CGCCGAGGGC GTCGAGCAGA TCCATGTCGC CGGCCCGGTG CAGACCAATA CGGCCGAGTC GCTCGCGCTC GCGATCCGCG ACGGTATCGG GATCGGCATG CTGCCGCTGT ATTCGGCGAT CGACGCGCTG CGCGACGGCA CGCTCGTGCG CGTGCTGCCC GAGCACATCC TGCAGAAGAT GAACGTGTAT GCGCTGTATC CGTCGCGCCG CTTCGTCGAC GCGAAGGTGC GCACCTGGGT CGAGATGCTG CGGGCGCAGG TGCCGGGGAT GATCGCGCGC GACGTCGAGA TGCTGAACGC GATCGACCGC GAACCGCAGG CCGCG
Details for BuamA.12800.u.A1.GE31799
HARVESTED ON: 3/8/2011
SEQUENCED ON: 3/14/2011
EXPECTED MW: 36kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 93
Validated AA Sequence
MDTLQNMRVF TRVVETGSFT AAAQSLNSTT GAMSRAVSEL EARLRTRLMN RSTRRLALTS AGESYLRRCR QILADVDRAE EEASCAHERP AGVLRMHSFA SVGHHYVMPA LTRYHTQYPD VSIELSLSQR MPDLFDGTSD MAVVTASSLP DSELVSHLLG STFSILCASP EYVKRHGAPA RPQDLGAHAC LTLCTPAFPT HEWVLEGAEG VEQIHVAGPV QTNTAESLAL AIRDGIGIGM LPLYSAIDAL RDGTLVRVLP EHILQKMNVY ALYPSRRFVD AKVRTWVEML RAQVPGMIAR DVEMLNAIDR EPQA
Validated NT Sequence
cgcggcctgc ggttcgcggt cgatcgcgtt cagcatctcg acgtcgcgcg cgatcatccc cggcacctgc gcccgcagca tctcgaccca ggtgcgcacc ttcgcgtcga cgaagcggcg cgacggatac agcgcataca cgttcatctt ctgcaggatg tgctcgggca gcacgcgcac gagcgtgccg tcgcgcagcg cgtcgatcgc cgaatacagc ggcagcatgc cgatcccgat accgtcgcgg atcgcgagcg cgagcgactc ggccgtattg gtctgcaccg ggccggcgac atggatctgc tcgacgccct cggcgccttc gagcacccat tcgtgagtcg ggaaggccgg cgtgcagagc gtcaggcatg cgtgcgcgcc gagatcctgc gggcgtgcgg gcgcgccgtg gcgcttcacg tactcgggcg acgcgcacag gatgctgaag gtcgagccca gcaggtgcga cacgagttcc gaatcgggca gcgacgacgc ggtcacgacc gccatgtcgc tcgtgccgtc gaacaggtcg ggcatgcgct gcgacagcga cagctcgatc gacacgtccg gatactgcgt gtgatagcgc gtcagcgcgg gcatcacgta gtgatggccg acgctcgcga agctgtgcat gcgcagcacg ccggctggcc gctcgtgcgc gcagcttgct tcttcctcgg cgcgatcgac gtcggcgagg atctgccggc agcgccgcag atagctttcg ccggccgacg tcagcgcgag acggcgtgtc gagcgattca tcagacgggt gcgcaggcgc gcctcgagct ccgacaccgc gcgcgacatc gcgcccgtcg tcgaattcag cgattgcgcg gccgcggtga aactgcccgt ctcgacgacg cgcgtaaaca cccgcatgtt ttgtagggta tccatcgaac caggaccctg ggtctgagct tccagggtac cc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDTLQNMRV FTRVVETGSF TAAAQSLNST TGAMSRAVSE LEARLRTRLM NRSTRRLALT SAGESYLRRC RQILADVDRA EEEASCAHER PAGVLRMHSF ASVGHHYVMP ALTRYHTQYP DVSIELSLSQ RMPDLFDGTS DMAVVTASSL PDSELVSHLL GSTFSILCAS PEYVKRHGAP ARPQDLGAHA CLTLCTPAFP THEWVLEGAE GVEQIHVAGP VQTNTAESLA LAIRDGIGIG MLPLYSAIDA LRDGTLVRVL PEHILQKMNV YALYPSRRFV DAKVRTWVEM LRAQVPGMIA RDVEMLNAID REPQAA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggatacccta caaaacatgc gggtgtttac gcgcgtcgtc gagacgggca gtttcaccgc ggccgcgcaa tcgctgaatt cgacgacggg cgcgatgtcg cgcgcggtgt cggagctcga ggcgcgcctg cgcacccgtc tgatgaatcg ctcgacacgc cgtctcgcgc tgacgtcggc cggcgaaagc tatctgcggc gctgccggca gatcctcgcc gacgtcgatc gcgccgagga agaagcaagc tgcgcgcacg agcggccagc cggcgtgctg cgcatgcaca gcttcgcgag cgtcggccat cactacgtga tgcccgcgct gacgcgctat cacacgcagt atccggacgt gtcgatcgag ctgtcgctgt cgcagcgcat gcccgacctg ttcgacggca cgagcgacat ggcggtcgtg accgcgtcgt cgctgcccga ttcggaactc gtgtcgcacc tgctgggctc gaccttcagc atcctgtgcg cgtcgcccga gtacgtgaag cgccacggcg cgcccgcacg cccgcaggat ctcggcgcgc acgcatgcct gacgctctgc acgccggcct tcccgactca cgaatgggtg ctcgaaggcg ccgagggcgt cgagcagatc catgtcgccg gcccggtgca gaccaatacg gccgagtcgc tcgcgctcgc gatccgcgac ggtatcggga tcggcatgct gccgctgtat tcggcgatcg acgcgctgcg cgacggcacg ctcgtgcgcg tgctgcccga gcacatcctg cagaagatga acgtgtatgc gctgtatccg tcgcgccgct tcgtcgacgc gaaggtgcgc acctgggtcg agatgctgcg ggcgcaggtg ccggggatga tcgcgcgcga cgtcgagatg ctgaacgcga tcgaccgcga accgcaggcc gcgtaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa