BuamA.12800.s

Transcriptional regulator, LysR family

CENTER ID: BuamA.12800.s
ORGANISM: Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.12800.s.A1.GE31797 Full length( BuamA.12800.s ) 1 295
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|398577.6.peg.5463
RefSeq: YP_001811913.1
UniProt: B1Z195

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTCFARAVET GSFSAAGRDL GLGQPNVSRY VAALEDHLQT RLLHRSTRKL ALTPEGERYY ADIRRILDAV DESESSFRNS VDPSGLLRVA CPTALAHAYV LPQVPVFLER YPRLTLDLQI SDRYVNLVDE GVELAIRIGH LEDSALRARR IGMYERVAVA SKDYLAKRGV PDTPDDLREH DCVVYTLLSS GATWRFRDAD IPVSGRLRVN SPEAVRAAVT AGLGVGHGPE WLFEEGLKSG RLQLILTGHF APAVPVQIVY VANRLLPTRA IVFIEFISDV FSRISAFNKI AFPKP
NT Sequence
ATGACGTGCT TCGCACGGGC CGTCGAGACG GGGAGTTTTT CTGCTGCAGG GCGAGATTTG GGATTGGGCC AACCCAACGT CAGCCGCTAT GTAGCCGCGC TGGAGGACCA TCTCCAAACG CGGCTGCTGC ACCGATCCAC ACGAAAGCTT GCCCTGACGC CCGAGGGTGA ACGCTACTAT GCAGATATCC GCCGGATTCT TGACGCGGTG GACGAATCTG AATCGTCTTT CAGGAACAGT GTCGATCCAT CGGGACTCTT GCGCGTGGCC TGTCCCACGG CGTTAGCGCA TGCATATGTG TTGCCTCAGG TCCCGGTCTT TCTGGAGCGG TATCCCAGAT TGACGCTGGA TCTTCAGATC AGCGATCGCT ACGTGAACCT TGTAGACGAG GGAGTCGAGC TCGCGATTCG CATTGGACAT CTGGAGGACA GTGCGCTACG CGCCAGGCGC ATAGGAATGT ACGAGCGTGT GGCCGTGGCC AGCAAGGACT ATCTGGCGAA ACGCGGAGTT CCGGACACAC CTGATGACCT GCGCGAACAT GATTGCGTGG TCTATACTTT GCTGTCGTCC GGTGCGACAT GGCGCTTTCG TGACGCCGAT ATTCCGGTCT CTGGGAGATT GCGGGTCAAC TCACCCGAGG CCGTTCGAGC AGCGGTCACA GCAGGGTTAG GGGTCGGGCA TGGGCCGGAA TGGCTGTTCG AGGAAGGGTT AAAAAGCGGT CGTTTGCAGT TAATTCTGAC CGGGCACTTT GCTCCGGCCG TCCCTGTTCA GATCGTCTAT GTTGCAAACA GGTTGCTTCC AACACGGGCC ATCGTGTTTA TTGAATTCAT TTCCGACGTA TTTTCAAGGA TTTCGGCTTT CAACAAAATC GCATTTCCGA AGCCT
Details for BuamA.12800.s.A1.GE31797
HARVESTED ON: 3/8/2011
SEQUENCED ON: 3/14/2011
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 87
PERCENT COVERAGE: 109
Validated AA Sequence
MXXXXHHHHH MGTLEAQTQG PGSMTCFARA VETGSFSAAG RDLGLGQPNV SRYVAALEDH LQTRLLHRST RKLALTPEGE RYYADIRRIL DAVDESESSF RNSVDPSGLL RVACPTALAH AYVLPQVPVF LERYPRLTLD LQISDRYVNL VDEGVELAIR IGHLEDSALR ARRIGMYERV AVASKDYLAK RGVPDTPDDL REHDCVVYTL LSSGATWRFR DAXIPVSGRL RVNSPEAVRA AVTAGLGVGH GPEWLFXEXL KSGRLQLILT GHFAPXVPVX IVYVAXXXLX HGXXXXXXHX RRIXXXXSXX XXXXXXKXXX XQXXXXXXXX XXXXXXXXXX XXXXXXX
Validated NT Sequence
cnnnnnnnnn nnngcggnnn ngccatgtnc ntgnnnnanc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgacgt gcttcgcacg ggccgtcgag acggggagtt tttctgctgc agggcgagat ttgggattgg gccaacccaa cgtcagccgc tatgtagccg cgctggagga ccatctccaa acgcggctgc tgcaccgatc cacacgaaag cttgccctga cgcccgaggg tgaacgctac tatgcagata tccgccggat tcttgacgcg gtggacgaat ctgaatcgtc tttcaggaac agtgtcgatc catcgggact cttgcgcgtg gcctgtccca cggcgttagc gcatgcatat gtgttgcctc aggtcccggt ctttctggag cggtatccca gattgacgct ggatcttcag atcagcgatc gctacgtgaa ccttgtagac gagggagtcg agctcgcgat tcgcattgga catctggagg acagtgcgct acgcgccagg cgcataggaa tgtacgagcg tgtggccgtg gccagcaagg actatctggc gaaacgcgga gttccggaca cacctgatga cctgcgcgaa catgattgcg tggtctatac tttgctgtcg tccggtgcga catggcgctt tcgtgacgcc natattccgg tctctgggag attgcgggtc aactcacccg aggccgttcg agcagcggtc acagcagggt taggggtcgg gcatgggccg gaatggctgt tcgangaang gttaaaaagc ggtcgtttgc agttaattct gaccgggcac tttgctccng ncgtccctgt nnanatcgtc tatgttgcaa annnnnngct tnnncacggg ncatnnnnnt nnnnnantca tttncgacgt atttnangan nnnnntcacn nnnnncnntt ncnnannntn ataaannnnn nnnnnngcag cnnntnnnnn nnnnnnnnnt nnnancnaan ncnnangnan nnnnnntnnn nnnnnnnnnn nnnnnnnnnn nnnnnnna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTCFARAVE TGSFSAAGRD LGLGQPNVSR YVAALEDHLQ TRLLHRSTRK LALTPEGERY YADIRRILDA VDESESSFRN SVDPSGLLRV ACPTALAHAY VLPQVPVFLE RYPRLTLDLQ ISDRYVNLVD EGVELAIRIG HLEDSALRAR RIGMYERVAV ASKDYLAKRG VPDTPDDLRE HDCVVYTLLS SGATWRFRDA DIPVSGRLRV NSPEAVRAAV TAGLGVGHGP EWLFEEGLKS GRLQLILTGH FAPAVPVQIV YVANRLLPTR AIVFIEFISD VFSRISAFNK IAFPKP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacgtgcttc gcacgggccg tcgagacggg gagtttttct gctgcagggc gagatttggg attgggccaa cccaacgtca gccgctatgt agccgcgctg gaggaccatc tccaaacgcg gctgctgcac cgatccacac gaaagcttgc cctgacgccc gagggtgaac gctactatgc agatatccgc cggattcttg acgcggtgga cgaatctgaa tcgtctttca ggaacagtgt cgatccatcg ggactcttgc gcgtggcctg tcccacggcg ttagcgcatg catatgtgtt gcctcaggtc ccggtctttc tggagcggta tcccagattg acgctggatc ttcagatcag cgatcgctac gtgaaccttg tagacgaggg agtcgagctc gcgattcgca ttggacatct ggaggacagt gcgctacgcg ccaggcgcat aggaatgtac gagcgtgtgg ccgtggccag caaggactat ctggcgaaac gcggagttcc ggacacacct gatgacctgc gcgaacatga ttgcgtggtc tatactttgc tgtcgtccgg tgcgacatgg cgctttcgtg acgccgatat tccggtctct gggagattgc gggtcaactc acccgaggcc gttcgagcag cggtcacagc agggttaggg gtcgggcatg ggccggaatg gctgttcgag gaagggttaa aaagcggtcg tttgcagtta attctgaccg ggcactttgc tccggccgtc cctgttcaga tcgtctatgt tgcaaacagg ttgcttccaa cacgggccat cgtgtttatt gaattcattt ccgacgtatt ttcaaggatt tcggctttca acaaaatcgc atttccgaag ccttaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa