BuamA.12800.r

Transcriptional regulator, LysR family

CENTER ID: BuamA.12800.r
ORGANISM: Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.12800.r.A1.GE31796 Full length( BuamA.12800.r ) 1 306
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.12800.r.A1.PW31703 Full length( BuamA.12800.r ) 1 306

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|398577.6.peg.3747
RefSeq: YP_001810317.1
UniProt: B1Z0I0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDYIDKLRIF RSVVEMRSFT RAADMHGLAR PVVSRAVADL EARFGSRLLH RTTRQVSLTE TAERIYERCA AVLDELDSLE AQAQSHTREP EGLLRLVAHT TAALNRLVPL IAGFKAAHPK VRLDVTLTER PVDLVGEGYD IGIVVPYMLT SETTVVRLVE RIPVVIVATP AYLRTHPRPE TPAALAEHAF VPLSPSLRRP ALSFRVDGDT LTVPLSFDIS SNSPVFNREM VMHDFGIGVV PRTLVEAELA SGALVQLLEG ADLVDAFVEI KLAYANRALL PAKVKAFIDY TAAYCDHDGR IVPAAA
NT Sequence
ATGGACTACA TCGACAAGCT GCGGATCTTC CGGTCGGTGG TGGAAATGCG CAGCTTCACG CGCGCCGCCG ACATGCACGG CCTCGCGCGG CCGGTCGTGT CGCGTGCAGT CGCGGATCTC GAGGCGCGCT TCGGCAGCCG CCTGCTGCAC CGGACCACGC GCCAGGTCTC GCTGACCGAA ACCGCCGAGC GCATCTACGA GCGCTGCGCG GCCGTGCTCG ACGAACTCGA CTCGCTGGAA GCGCAGGCGC AGTCGCACAC GCGCGAGCCC GAGGGGCTGC TGCGCCTCGT CGCGCACACG ACGGCCGCGC TGAACCGGCT CGTGCCGCTG ATCGCCGGCT TCAAGGCCGC GCATCCGAAG GTGCGCCTCG ACGTCACGCT GACCGAGCGC CCGGTCGACC TCGTCGGCGA AGGCTACGAC ATCGGCATCG TCGTGCCGTA CATGCTGACG AGCGAGACGA CCGTGGTGCG CCTGGTCGAA CGCATTCCGG TGGTGATCGT CGCGACGCCC GCGTATCTGC GTACGCATCC GCGTCCCGAG ACGCCGGCCG CGCTCGCCGA GCATGCGTTC GTGCCGCTGT CGCCGTCGCT GCGCCGGCCC GCGCTGTCGT TCCGCGTCGA CGGCGACACG CTGACCGTGC CGTTGAGCTT CGACATCTCG TCGAACAGCC CCGTCTTCAA CCGTGAAATG GTGATGCACG ACTTCGGCAT CGGTGTCGTG CCGAGAACGC TCGTCGAGGC CGAACTCGCG TCGGGCGCGC TCGTGCAGCT GCTCGAAGGC GCCGATCTCG TCGACGCATT CGTGGAGATC AAGCTCGCTT ACGCGAACCG CGCGCTGCTG CCCGCGAAGG TCAAGGCGTT CATCGACTAC ACGGCCGCGT ACTGCGACCA CGACGGCCGG ATTGTTCCCG CCGCCGCG
Details for BuamA.12800.r.A1.GE31796
HARVESTED ON: 3/8/2011
SEQUENCED ON: 3/14/2011
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMDYIDKL RIFRSVVEMR SFTRAADMHG LARPVVSRAV ADLEARFGSR LLHRTTRQVS LTETAERIYE RCAAVLDELD SLEAQAQSHT REPEGLLRLV AHTTAALNRL VPLIAGFKAA HPKVRLDVTL TERPVDLVGE GYDIGIVVPY MLTSETTVVR LVERIPVVIV ATPAYLRTHP RPETPAALAE HAFVPLSPSL RRPALSFRVD GDTLTVPLSF DISSNSPVFN REMVMHDFGI GVVPRTLVEA ELASGALVQL LEGADLVDAF VEIKLAYANR ALLPAKVKAF IDYTAAYCDH DGRIVPAAA
Validated NT Sequence
ttattacgcg gcggcgggaa caatccggcc gtcgtggtcg cagtacgcgg ccgtgtagtc gatgaacgcc ttgaccttcg cgggcagcag cgcgcggttc gcgtaagcga gcttgatctc cacgaatgcg tcgacgagat cggcgccttc gagcagctgc acgagcgcgc ccgacgcgag ttcggcctcg acgagcgttc tcggcacgac accgatgccg aagtcgtgca tcaccatttc acggttgaag acggggctgt tcgacgagat gtcgaagctc aacggcacgg tcagcgtgtc gccgtcgacg cggaacgaca gcgcgggccg gcgcagcgac ggcgacagcg gcacgaacgc atgctcggcg agcgcggccg gcgtctcggg acgcggatgc gtacgcagat acgcgggcgt cgcgacgatc accaccggaa tgcgttcgac caggcgcacc acggtcgtct cgctcgtcag catgtacggc acgacgatgc cgatgtcgta gccttcgccg acgaggtcga ccgggcgctc ggtcagcgtg acgtcgaggc gcaccttcgg atgcgcggcc ttgaagccgg cgatcagcgg cacgagccgg ttcagcgcgg ccgtcgtgtg cgcgacgagg cgcagcagcc cctcgggctc gcgcgtgtgc gactgcgcct gcgcttccag cgagtcgagt tcgtcgagca cggccgcgca gcgctcgtag atgcgctcgg cggtttcggt cagcgagacc tggcgcgtgg tccggtgcag caggcggctg ccgaagcgcg cctcgagatc cgcgactgca cgcgacacga ccggccgcgc gaggccgtgc atgtcggcgg cgcgcgtgaa gctgcgcatt tccaccaccg accggaagat ccgcagcttg tcgatgtagt ccatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDYIDKLRI FRSVVEMRSF TRAADMHGLA RPVVSRAVAD LEARFGSRLL HRTTRQVSLT ETAERIYERC AAVLDELDSL EAQAQSHTRE PEGLLRLVAH TTAALNRLVP LIAGFKAAHP KVRLDVTLTE RPVDLVGEGY DIGIVVPYML TSETTVVRLV ERIPVVIVAT PAYLRTHPRP ETPAALAEHA FVPLSPSLRR PALSFRVDGD TLTVPLSFDI SSNSPVFNRE MVMHDFGIGV VPRTLVEAEL ASGALVQLLE GADLVDAFVE IKLAYANRAL LPAKVKAFID YTAAYCDHDG RIVPAAA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggactacatc gacaagctgc ggatcttccg gtcggtggtg gaaatgcgca gcttcacgcg cgccgccgac atgcacggcc tcgcgcggcc ggtcgtgtcg cgtgcagtcg cggatctcga ggcgcgcttc ggcagccgcc tgctgcaccg gaccacgcgc caggtctcgc tgaccgaaac cgccgagcgc atctacgagc gctgcgcggc cgtgctcgac gaactcgact cgctggaagc gcaggcgcag tcgcacacgc gcgagcccga ggggctgctg cgcctcgtcg cgcacacgac ggccgcgctg aaccggctcg tgccgctgat cgccggcttc aaggccgcgc atccgaaggt gcgcctcgac gtcacgctga ccgagcgccc ggtcgacctc gtcggcgaag gctacgacat cggcatcgtc gtgccgtaca tgctgacgag cgagacgacc gtggtgcgcc tggtcgaacg cattccggtg gtgatcgtcg cgacgcccgc gtatctgcgt acgcatccgc gtcccgagac gccggccgcg ctcgccgagc atgcgttcgt gccgctgtcg ccgtcgctgc gccggcccgc gctgtcgttc cgcgtcgacg gcgacacgct gaccgtgccg ttgagcttcg acatctcgtc gaacagcccc gtcttcaacc gtgaaatggt gatgcacgac ttcggcatcg gtgtcgtgcc gagaacgctc gtcgaggccg aactcgcgtc gggcgcgctc gtgcagctgc tcgaaggcgc cgatctcgtc gacgcattcg tggagatcaa gctcgcttac gcgaaccgcg cgctgctgcc cgcgaaggtc aaggcgttca tcgactacac ggccgcgtac tgcgaccacg acggccggat tgttcccgcc gccgcgtaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for BuamA.12800.r.A1.PW31703
PURIFICATION DATe: 7/15/2011
CONCENTRATION: 30.63mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 50µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMDYIDKL RIFRSVVEMR SFTRAADMHG LARPVVSRAV ADLEARFGSR LLHRTTRQVS LTETAERIYE RCAAVLDELD SLEAQAQSHT REPEGLLRLV AHTTAALNRL VPLIAGFKAA HPKVRLDVTL TERPVDLVGE GYDIGIVVPY MLTSETTVVR LVERIPVVIV ATPAYLRTHP RPETPAALAE HAFVPLSPSL RRPALSFRVD GDTLTVPLSF DISSNSPVFN REMVMHDFGI GVVPRTLVEA ELASGALVQL LEGADLVDAF VEIKLAYANR ALLPAKVKAF IDYTAAYCDH DGRIVPAAA
Validated NT Sequence
ttattacgcg gcggcgggaa caatccggcc gtcgtggtcg cagtacgcgg ccgtgtagtc gatgaacgcc ttgaccttcg cgggcagcag cgcgcggttc gcgtaagcga gcttgatctc cacgaatgcg tcgacgagat cggcgccttc gagcagctgc acgagcgcgc ccgacgcgag ttcggcctcg acgagcgttc tcggcacgac accgatgccg aagtcgtgca tcaccatttc acggttgaag acggggctgt tcgacgagat gtcgaagctc aacggcacgg tcagcgtgtc gccgtcgacg cggaacgaca gcgcgggccg gcgcagcgac ggcgacagcg gcacgaacgc atgctcggcg agcgcggccg gcgtctcggg acgcggatgc gtacgcagat acgcgggcgt cgcgacgatc accaccggaa tgcgttcgac caggcgcacc acggtcgtct cgctcgtcag catgtacggc acgacgatgc cgatgtcgta gccttcgccg acgaggtcga ccgggcgctc ggtcagcgtg acgtcgaggc gcaccttcgg atgcgcggcc ttgaagccgg cgatcagcgg cacgagccgg ttcagcgcgg ccgtcgtgtg cgcgacgagg cgcagcagcc cctcgggctc gcgcgtgtgc gactgcgcct gcgcttccag cgagtcgagt tcgtcgagca cggccgcgca gcgctcgtag atgcgctcgg cggtttcggt cagcgagacc tggcgcgtgg tccggtgcag caggcggctg ccgaagcgcg cctcgagatc cgcgactgca cgcgacacga ccggccgcgc gaggccgtgc atgtcggcgg cgcgcgtgaa gctgcgcatt tccaccaccg accggaagat ccgcagcttg tcgatgtagt ccatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDYIDKLRI FRSVVEMRSF TRAADMHGLA RPVVSRAVAD LEARFGSRLL HRTTRQVSLT ETAERIYERC AAVLDELDSL EAQAQSHTRE PEGLLRLVAH TTAALNRLVP LIAGFKAAHP KVRLDVTLTE RPVDLVGEGY DIGIVVPYML TSETTVVRLV ERIPVVIVAT PAYLRTHPRP ETPAALAEHA FVPLSPSLRR PALSFRVDGD TLTVPLSFDI SSNSPVFNRE MVMHDFGIGV VPRTLVEAEL ASGALVQLLE GADLVDAFVE IKLAYANRAL LPAKVKAFID YTAAYCDHDG RIVPAAA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGACTACATC GACAAGCTGC GGATCTTCCG GTCGGTGGTG GAAATGCGCA GCTTCACGCG CGCCGCCGAC ATGCACGGCC TCGCGCGGCC GGTCGTGTCG CGTGCAGTCG CGGATCTCGA GGCGCGCTTC GGCAGCCGCC TGCTGCACCG GACCACGCGC CAGGTCTCGC TGACCGAAAC CGCCGAGCGC ATCTACGAGC GCTGCGCGGC CGTGCTCGAC GAACTCGACT CGCTGGAAGC GCAGGCGCAG TCGCACACGC GCGAGCCCGA GGGGCTGCTG CGCCTCGTCG CGCACACGAC GGCCGCGCTG AACCGGCTCG TGCCGCTGAT CGCCGGCTTC AAGGCCGCGC ATCCGAAGGT GCGCCTCGAC GTCACGCTGA CCGAGCGCCC GGTCGACCTC GTCGGCGAAG GCTACGACAT CGGCATCGTC GTGCCGTACA TGCTGACGAG CGAGACGACC GTGGTGCGCC TGGTCGAACG CATTCCGGTG GTGATCGTCG CGACGCCCGC GTATCTGCGT ACGCATCCGC GTCCCGAGAC GCCGGCCGCG CTCGCCGAGC ATGCGTTCGT GCCGCTGTCG CCGTCGCTGC GCCGGCCCGC GCTGTCGTTC CGCGTCGACG GCGACACGCT GACCGTGCCG TTGAGCTTCG ACATCTCGTC GAACAGCCCC GTCTTCAACC GTGAAATGGT GATGCACGAC TTCGGCATCG GTGTCGTGCC GAGAACGCTC GTCGAGGCCG AACTCGCGTC GGGCGCGCTC GTGCAGCTGC TCGAAGGCGC CGATCTCGTC GACGCATTCG TGGAGATCAA GCTCGCTTAC GCGAACCGCG CGCTGCTGCC CGCGAAGGTC AAGGCGTTCA TCGACTACAC GGCCGCGTAC TGCGACCACG ACGGCCGGAT TGTTCCCGCC GCCGCGTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA