BuamA.12800.n

Transcriptional regulator, LysR family

CENTER ID: BuamA.12800.n
ORGANISM: Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.12800.n.A1.GE31792 Full length( BuamA.12800.n ) 1 300
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|398577.6.peg.4844
RefSeq: YP_001811342.1
UniProt: B1YXZ2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKTTLDEMLA FVTIADTGSL TGAADRLHQP TSVMSRILRR LEDKLDTTLI RRTTRRLELT EEGNVFLDYA RDIIASVEFA EDQIARQRET PAGRLRINAA MPFMQHVIAP MMPGFREAYP AIEAELHTSD EIIDLLEQRT DVAIRIGELR DSTLHARKLG SSRLRILASP AYLERAGIPE SVDDLATHTL LGFVQPETLN HWPLRTAAGD GYRVQPALQA SSGETLLTLA LNGAGIVCLA DFMTGTHRRD GSLVELLVDD TTEFRQPIHA VYYRNATLSS RIVSFLDYVG AQLKESGDID
NT Sequence
ATGAAGACCA CACTAGACGA AATGCTCGCG TTCGTAACGA TCGCAGACAC GGGTTCGCTG ACGGGTGCGG CGGACCGGTT GCATCAGCCG ACCTCCGTGA TGAGCCGAAT CCTGCGGCGG CTGGAAGATA AACTCGACAC AACGCTGATC AGGCGCACGA CACGCCGGCT GGAACTCACG GAAGAGGGCA ACGTCTTCCT CGACTATGCG CGAGACATTA TTGCGTCGGT CGAGTTTGCC GAAGACCAGA TCGCCCGGCA GCGCGAAACG CCGGCGGGCC GTCTGCGGAT CAACGCTGCG ATGCCGTTCA TGCAGCACGT GATCGCGCCG ATGATGCCGG GGTTTCGCGA AGCCTATCCG GCGATCGAGG CCGAGCTTCA TACGAGCGAC GAGATCATCG ACTTGCTCGA ACAACGCACC GATGTCGCGA TCCGGATCGG TGAGTTGCGC GACTCGACAC TGCACGCGCG CAAGCTCGGC AGCAGCAGGC TACGTATTCT TGCCAGCCCG GCCTATCTGG AGAGGGCCGG GATACCCGAG AGCGTGGACG ATCTTGCGAC CCATACGCTG CTCGGCTTCG TCCAGCCGGA GACGCTGAAC CACTGGCCGT TGCGTACTGC GGCTGGCGAT GGGTACCGGG TACAGCCTGC GCTGCAGGCG TCGAGCGGCG AGACGCTGCT GACGCTCGCG CTCAATGGCG CCGGCATCGT GTGCCTGGCG GATTTCATGA CGGGTACGCA TCGCCGCGAC GGCAGTCTGG TCGAACTACT CGTGGACGAC ACCACCGAAT TTCGCCAGCC GATTCATGCG GTCTACTATC GCAACGCGAC GCTGTCGTCA CGCATCGTCA GCTTTCTTGA CTACGTCGGT GCACAGTTGA AGGAAAGCGG CGACATCGAC
Details for BuamA.12800.n.A1.GE31792
HARVESTED ON: 3/8/2011
SEQUENCED ON: 3/14/2011
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMKTTLDE MLAFVTIADT GSLTGAADRL HQPTSVMSRI LRRLEDKLDT TLIRRTTRRL ELTEEGNVFL DYARDIIASV EFAEDQIARQ RETPAGRLRI NAAMPFMQHV IAPMMPGFRE AYPAIEAELH TSDEIIDLLE QRTDVAIRIG ELRDSTLHAR KLGSSRLRIL ASPAYLERAG IPESVDDLAT HTLLGFVQPE TLNHWPLRTA AGDGYRVQPA LQASSGETLL TLALNGAGIV CLADFMTGTH RRDGSLVELL VDDTTEFRQP IHAVYYRNAT LSSRIVSFLD YVGAQLKESG DID
Validated NT Sequence
ttattagtcg atgtcgccgc tttccttcaa ctgtgcaccg acgtagtcaa gaaagctgac gatgcgtgac gacagcgtcg cgttgcgata gtagaccgca tgaatcggct ggcgaaattc ggtggtgtcg tccacgagta gttcgaccag actgccgtcg cggcgatgcg tacccgtcat gaaatccgcc aggcacacga tgccggcgcc attgagcgcg agcgtcagca gcgtctcgcc gctcgacgcc tgcagcgcag gctgtacccg gtacccatcg ccagccgcag tacgcaacgg ccagtggttc agcgtctccg gctggacgaa gccgagcagc gtatgggtcg caagatcgtc cacgctctcg ggtatcccgg ccctctccag ataggccggg ctggcaagaa tacgtagcct gctgctgccg agcttgcgcg cgtgcagtgt cgagtcgcgc aactcaccga tccggatcgc gacatcggtg cgttgttcga gcaagtcgat gatctcgtcg ctcgtatgaa gctcggcctc gatcgccgga taggcttcgc gaaaccccgg catcatcggc gcgatcacgt gctgcatgaa cggcatcgca gcgttgatcc gcagacggcc cgccggcgtt tcgcgctgcc gggcgatctg gtcttcggca aactcgaccg acgcaataat gtctcgcgca tagtcgagga agacgttgcc ctcttccgtg agttccagcc ggcgtgtcgt gcgcctgatc agcgttgtgt cgagtttatc ttccagccgc cgcaggattc ggctcatcac ggaggtcggc tgatgcaacc ggtccgccgc acccgtcagc gaacccgtgt ctgcgatcgt tacgaacgcg agcatttcgt ctagtgtggt cttcatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKTTLDEML AFVTIADTGS LTGAADRLHQ PTSVMSRILR RLEDKLDTTL IRRTTRRLEL TEEGNVFLDY ARDIIASVEF AEDQIARQRE TPAGRLRINA AMPFMQHVIA PMMPGFREAY PAIEAELHTS DEIIDLLEQR TDVAIRIGEL RDSTLHARKL GSSRLRILAS PAYLERAGIP ESVDDLATHT LLGFVQPETL NHWPLRTAAG DGYRVQPALQ ASSGETLLTL ALNGAGIVCL ADFMTGTHRR DGSLVELLVD DTTEFRQPIH AVYYRNATLS SRIVSFLDYV GAQLKESGDI D
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaagaccaca ctagacgaaa tgctcgcgtt cgtaacgatc gcagacacgg gttcgctgac gggtgcggcg gaccggttgc atcagccgac ctccgtgatg agccgaatcc tgcggcggct ggaagataaa ctcgacacaa cgctgatcag gcgcacgaca cgccggctgg aactcacgga agagggcaac gtcttcctcg actatgcgcg agacattatt gcgtcggtcg agtttgccga agaccagatc gcccggcagc gcgaaacgcc ggcgggccgt ctgcggatca acgctgcgat gccgttcatg cagcacgtga tcgcgccgat gatgccgggg tttcgcgaag cctatccggc gatcgaggcc gagcttcata cgagcgacga gatcatcgac ttgctcgaac aacgcaccga tgtcgcgatc cggatcggtg agttgcgcga ctcgacactg cacgcgcgca agctcggcag cagcaggcta cgtattcttg ccagcccggc ctatctggag agggccggga tacccgagag cgtggacgat cttgcgaccc atacgctgct cggcttcgtc cagccggaga cgctgaacca ctggccgttg cgtactgcgg ctggcgatgg gtaccgggta cagcctgcgc tgcaggcgtc gagcggcgag acgctgctga cgctcgcgct caatggcgcc ggcatcgtgt gcctggcgga tttcatgacg ggtacgcatc gccgcgacgg cagtctggtc gaactactcg tggacgacac caccgaattt cgccagccga ttcatgcggt ctactatcgc aacgcgacgc tgtcgtcacg catcgtcagc tttcttgact acgtcggtgc acagttgaag gaaagcggcg acatcgacta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a