BuamA.12800.l

Transcriptional regulator, LysR family

CENTER ID: BuamA.12800.l
ORGANISM: Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.12800.l.A1.GE31790 Full length( BuamA.12800.l ) 1 322
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|398577.6.peg.4804
RefSeq: YP_001811303.1
UniProt: B1YXM5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDTLLSMRIF SHVVETGSFS AAADHMEFSK ACVSRAVSTL ENRLRARLLN RTTRSLSLTE SGQRYYARCK QILADLDDAE AQAGGAYTVP HGTLHVHCSP ELGLRRFTQC LVEYQRQHPS VKLHVTFIPG AAHLINNRVD VSIVPTASLP DSGNVSRMVG QIEHVLVAEP TYLQACRIDT FEQIAPHALT PIPARVATPQ RHARLTIVKP ASANNDGPFV IDDVDATRFA VLAGAGVAAL PLHCVIDDIR QGALVRLFPD HRLERTPVFV LYASRRHVDA KIRTFVDFMA ARLIDVLQGP SCPTTADTSD TTACLDHPAR VG
NT Sequence
ATGGACACGC TACTGAGCAT GCGGATCTTC TCGCATGTGG TGGAAACGGG CAGTTTCAGT GCGGCTGCCG ACCATATGGA GTTCAGCAAG GCGTGCGTGT CGCGGGCGGT CTCCACGCTC GAGAACCGGT TGCGCGCGAG ACTGCTGAAC CGCACGACAC GCAGCCTGTC GTTGACGGAG TCCGGGCAGC GCTATTACGC CAGATGCAAG CAGATTCTTG CCGATCTGGA CGATGCCGAA GCGCAGGCCG GCGGCGCCTA TACCGTGCCG CACGGCACCC TGCACGTCCA TTGTTCGCCC GAGCTCGGAC TCCGGCGCTT CACGCAATGC CTCGTCGAAT ATCAACGCCA GCATCCGAGC GTCAAGCTTC ATGTCACGTT CATACCCGGC GCCGCACATC TGATCAACAA CCGCGTCGAT GTGTCCATCG TGCCCACGGC GTCACTGCCC GACTCCGGGA ACGTGAGCCG GATGGTCGGT CAGATCGAGC ATGTGCTGGT GGCCGAACCG ACGTATCTTC AGGCCTGCCG AATCGACACA TTCGAGCAGA TAGCCCCGCA CGCGCTGACG CCCATACCCG CGCGCGTTGC GACGCCGCAG CGCCACGCCA GGCTTACCAT CGTCAAGCCC GCATCGGCGA ACAACGACGG ACCGTTCGTC ATCGACGACG TCGACGCCAC GCGCTTCGCC GTTCTTGCGG GCGCGGGCGT CGCCGCGCTG CCGCTGCACT GCGTGATCGA CGACATTCGA CAGGGCGCGC TCGTGCGGCT GTTCCCCGAC CATCGCCTGG AGCGCACGCC CGTGTTCGTT CTTTACGCGT CGCGGCGTCA CGTCGACGCG AAGATCCGAA CGTTCGTCGA TTTCATGGCG GCGCGCCTGA TTGACGTGCT TCAGGGGCCC TCGTGCCCCA CTACCGCAGA TACGTCGGAC ACCACCGCTT GCCTCGACCA TCCCGCGCGC GTGGGC
Details for BuamA.12800.l.A1.GE31790
HARVESTED ON: 3/8/2011
SEQUENCED ON: 3/14/2011
EXPECTED MW: 37kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 93
Validated AA Sequence
MDTLLSMRIF SHVVETGSFS AAADHMEFSK ACVSRAVSTL ENRLRARLLN RTTRSLSLTE SGQRYYARCK QILADLDDAE AQAGGAYTVP HGTLHVHCSP ELGLRRFTQC LVEYQRQHPS VKLHVTFIPG AAHLINNRVD VSIVPTASLP DSGNVSRMVG QIEHVLVAEP TYLQACRIDT FEQIAPHALT PIPARVATPQ RHARLTIVKP ASANNDGPFV IDDVDATRFA VLAGAGVAAL PLHCVIDDIR QGALVRLFPD HRLERTPVFV LYASRRHVDA KIRTFVDFMA ARLIDVLQGP SCPTTADTSD TTACLDHPAR V
Validated NT Sequence
gcccacgcgc gcgggatggt cgaggcaagc ggtggtgtcc gacgtatctg cggtagtggg gcacgagggc ccctgaagca cgtcaatcag gcgcgccgcc atgaaatcga cgaacgttcg gatcttcgcg tcgacgtgac gccgcgacgc gtaaagaacg aacacgggcg tgcgctccag gcgatggtcg gggaacagcc gcacgagcgc gccctgtcga atgtcgtcga tcacgcagtg cagcggcagc gcggcgacgc ccgcgcccgc aagaacggcg aagcgcgtgg cgtcgacgtc gtcgatgacg aacggtccgt cgttgttcgc cgatgcgggc ttgacgatgg taagcctggc gtggcgctgc ggcgtcgcaa cgcgcgcggg tatgggcgtc agcgcgtgcg gggctatctg ctcgaatgtg tcgattcggc aggcctgaag atacgtcggt tcggccacca gcacatgctc gatctgaccg accatccggc tcacgttccc ggagtcgggc agtgacgccg tgggcacgat ggacacatcg acgcggttgt tgatcagatg tgcggcgccg ggtatgaacg tgacatgaag cttgacgctc ggatgctggc gttgatattc gacgaggcat tgcgtgaagc gccggagtcc gagctcgggc gaacaatgga cgtgcagggt gccgtgcggc acggtatagg cgccgccggc ctgcgcttcg gcatcgtcca gatcggcaag aatctgcttg catctggcgt aatagcgctg cccggactcc gtcaacgaca ggctgcgtgt cgtgcggttc agcagtctcg cgcgcaaccg gttctcgagc gtggagaccg cccgcgacac gcacgccttg ctgaactcca tatggtcggc agccgcactg aaactgcccg tttccaccac atgcgagaag atccgcatgc tcagtagcgt gtccatcgaa ccaggaccct gggtctgagc ttccagggta ccc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDTLLSMRI FSHVVETGSF SAAADHMEFS KACVSRAVST LENRLRARLL NRTTRSLSLT ESGQRYYARC KQILADLDDA EAQAGGAYTV PHGTLHVHCS PELGLRRFTQ CLVEYQRQHP SVKLHVTFIP GAAHLINNRV DVSIVPTASL PDSGNVSRMV GQIEHVLVAE PTYLQACRID TFEQIAPHAL TPIPARVATP QRHARLTIVK PASANNDGPF VIDDVDATRF AVLAGAGVAA LPLHCVIDDI RQGALVRLFP DHRLERTPVF VLYASRRHVD AKIRTFVDFM AARLIDVLQG PSCPTTADTS DTTACLDHPA RVG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacacgcta ctgagcatgc ggatcttctc gcatgtggtg gaaacgggca gtttcagtgc ggctgccgac catatggagt tcagcaaggc gtgcgtgtcg cgggcggtct ccacgctcga gaaccggttg cgcgcgagac tgctgaaccg cacgacacgc agcctgtcgt tgacggagtc cgggcagcgc tattacgcca gatgcaagca gattcttgcc gatctggacg atgccgaagc gcaggccggc ggcgcctata ccgtgccgca cggcaccctg cacgtccatt gttcgcccga gctcggactc cggcgcttca cgcaatgcct cgtcgaatat caacgccagc atccgagcgt caagcttcat gtcacgttca tacccggcgc cgcacatctg atcaacaacc gcgtcgatgt gtccatcgtg cccacggcgt cactgcccga ctccgggaac gtgagccgga tggtcggtca gatcgagcat gtgctggtgg ccgaaccgac gtatcttcag gcctgccgaa tcgacacatt cgagcagata gccccgcacg cgctgacgcc catacccgcg cgcgttgcga cgccgcagcg ccacgccagg cttaccatcg tcaagcccgc atcggcgaac aacgacggac cgttcgtcat cgacgacgtc gacgccacgc gcttcgccgt tcttgcgggc gcgggcgtcg ccgcgctgcc gctgcactgc gtgatcgacg acattcgaca gggcgcgctc gtgcggctgt tccccgacca tcgcctggag cgcacgcccg tgttcgttct ttacgcgtcg cggcgtcacg tcgacgcgaa gatccgaacg ttcgtcgatt tcatggcggc gcgcctgatt gacgtgcttc aggggccctc gtgccccact accgcagata cgtcggacac caccgcttgc ctcgaccatc ccgcgcgcgt gggctaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa