BuamA.12800.j

Transcriptional regulator, LysR family

CENTER ID: BuamA.12800.j
ORGANISM: Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.12800.j.A1.GE31788 Full length( BuamA.12800.j ) 1 322
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.12800.j.A1.PW31695 Full length( BuamA.12800.j ) 1 322

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|398577.6.peg.4588
RefSeq: YP_001811098.1
UniProt: B1YX20

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDMLENMRLF VRVVEAGSFT AVAKEIDATT AQVSRAVSNL EAHVQTRLLH RTTRHLGLTE SGERYFERAK SILAEIDYAN AEARNALLRP SGKMRIHAMT GLGQSHVVSS IVRYQEDNPD VSVELTLAQR MPNLVEEGYD VSIVTASQLP DSGYVAQTCG TSCSVLVASR DYLARHGTPK TPDDLPNHVC LRLDTPASPA SEWRLERGDD EETVYELQPA PFQVNVPDAL CVAVRAGRGI ACVALYTVLD DLREGRLIRV LPEYRLQTVS VYAVYATRRY LDAKIRTFLD HLRTTLTPAL ENDLRELDRL TVEHTPGKRQ RA
NT Sequence
ATGGACATGC TCGAAAACAT GCGCCTGTTC GTGCGCGTTG TCGAAGCCGG CAGTTTCACC GCGGTCGCGA AAGAAATCGA CGCGACCACC GCGCAGGTGT CGCGCGCGGT TTCGAATCTC GAAGCCCACG TGCAGACACG CCTGCTGCAT CGCACGACGC GCCATCTCGG TCTCACCGAA AGCGGCGAGC GCTATTTCGA GCGGGCGAAA TCGATCCTCG CCGAAATCGA CTACGCGAAC GCCGAAGCGC GCAACGCGCT GCTGCGGCCG AGCGGCAAGA TGCGCATCCA TGCGATGACG GGGCTCGGGC AGAGTCATGT CGTGTCGTCG ATCGTGCGCT ATCAGGAAGA CAACCCGGAC GTGTCGGTCG AGCTGACGCT CGCGCAGCGG ATGCCGAACC TCGTCGAGGA GGGCTACGAC GTGTCGATCG TGACCGCGTC GCAGTTGCCC GATTCCGGCT ACGTCGCGCA GACCTGCGGC ACGAGCTGCA GCGTGCTGGT CGCATCGCGC GACTACCTCG CGCGGCACGG CACGCCGAAG ACGCCGGACG ACCTGCCGAA CCATGTGTGC CTGCGCCTCG ATACGCCCGC GTCGCCGGCC AGCGAGTGGC GGCTCGAGCG CGGCGACGAC GAGGAGACCG TCTACGAACT GCAGCCGGCG CCGTTCCAGG TCAACGTGCC GGATGCGCTG TGCGTCGCGG TGCGGGCGGG GCGCGGCATC GCGTGCGTCG CGCTGTACAC GGTGCTCGAC GACCTTCGCG AAGGGCGGCT GATCCGCGTG CTGCCCGAGT ACCGCCTGCA GACGGTCAGC GTGTACGCCG TCTATGCGAC GCGCCGCTAT CTGGACGCGA AGATCCGCAC CTTCCTCGAC CATCTGCGCA CGACGCTCAC GCCCGCGCTG GAGAACGACC TGCGCGAACT CGACCGGCTC ACGGTCGAGC ACACGCCGGG CAAGCGCCAG CGCGCG
Details for BuamA.12800.j.A1.GE31788
HARVESTED ON: 3/8/2011
SEQUENCED ON: 3/14/2011
EXPECTED MW: 38kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMDMLENM RLFVRVVEAG SFTAVAKEID ATTAQVSRAV SNLEAHVQTR LLHRTTRHLG LTESGERYFE RAKSILAEID YANAEARNAL LRPSGKMRIH AMTGLGQSHV VSSIVRYQED NPDVSVELTL AQRMPNLVEE GYDVSIVTAS QLPDSGYVAQ TCGTSCSVLV ASRDYLARHG TPKTPDDLPN HVCLRLDTPA SPASEWRLER GDDEETVYEL QPAPFQVNVP DALCVAVRAG RGIACVALYT VLDDLREGRL IRVLPEYRLQ TVSVYAVYAT RRYLDAKIRT FLDHLRTTLT PALENDLREL DRLTVEHTPG KRQRA
Validated NT Sequence
ttattacgcg cgctggcgct tgcccggcgt gtgctcgacc gtgagccggt cgagttcgcg caggtcgttc tccagcgcgg gcgtgagcgt cgtgcgcaga tggtcgagga aggtgcggat cttcgcgtcc agatagcggc gcgtcgcata gacggcgtac acgctgaccg tctgcaggcg gtactcgggc agcacgcgga tcagccgccc ttcgcgaagg tcgtcgagca ccgtgtacag cgcgacgcac gcgatgccgc gccccgcccg caccgcgacg cacagcgcat ccggcacgtt gacctggaac ggcgccggct gcagttcgta gacggtctcc tcgtcgtcgc cgcgctcgag ccgccactcg ctggccggcg acgcgggcgt atcgaggcgc aggcacacat ggttcggcag gtcgtccggc gtcttcggcg tgccgtgccg cgcgaggtag tcgcgcgatg cgaccagcac gctgcagctc gtgccgcagg tctgcgcgac gtagccggaa tcgggcaact gcgacgcggt cacgatcgac acgtcgtagc cctcctcgac gaggttcggc atccgctgcg cgagcgtcag ctcgaccgac acgtccgggt tgtcttcctg atagcgcacg atcgacgaca cgacatgact ctgcccgagc cccgtcatcg catggatgcg catcttgccg ctcggccgca gcagcgcgtt gcgcgcttcg gcgttcgcgt agtcgatttc ggcgaggatc gatttcgccc gctcgaaata gcgctcgccg ctttcggtga gaccgagatg gcgcgtcgtg cgatgcagca ggcgtgtctg cacgtgggct tcgagattcg aaaccgcgcg cgacacctgc gcggtggtcg cgtcgatttc tttcgcgacc gcggtgaaac tgccggcttc gacaacgcgc acgaacaggc gcatgttttc gagcatgtcc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDMLENMRL FVRVVEAGSF TAVAKEIDAT TAQVSRAVSN LEAHVQTRLL HRTTRHLGLT ESGERYFERA KSILAEIDYA NAEARNALLR PSGKMRIHAM TGLGQSHVVS SIVRYQEDNP DVSVELTLAQ RMPNLVEEGY DVSIVTASQL PDSGYVAQTC GTSCSVLVAS RDYLARHGTP KTPDDLPNHV CLRLDTPASP ASEWRLERGD DEETVYELQP APFQVNVPDA LCVAVRAGRG IACVALYTVL DDLREGRLIR VLPEYRLQTV SVYAVYATRR YLDAKIRTFL DHLRTTLTPA LENDLRELDR LTVEHTPGKR QRA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacatgctc gaaaacatgc gcctgttcgt gcgcgttgtc gaagccggca gtttcaccgc ggtcgcgaaa gaaatcgacg cgaccaccgc gcaggtgtcg cgcgcggttt cgaatctcga agcccacgtg cagacacgcc tgctgcatcg cacgacgcgc catctcggtc tcaccgaaag cggcgagcgc tatttcgagc gggcgaaatc gatcctcgcc gaaatcgact acgcgaacgc cgaagcgcgc aacgcgctgc tgcggccgag cggcaagatg cgcatccatg cgatgacggg gctcgggcag agtcatgtcg tgtcgtcgat cgtgcgctat caggaagaca acccggacgt gtcggtcgag ctgacgctcg cgcagcggat gccgaacctc gtcgaggagg gctacgacgt gtcgatcgtg accgcgtcgc agttgcccga ttccggctac gtcgcgcaga cctgcggcac gagctgcagc gtgctggtcg catcgcgcga ctacctcgcg cggcacggca cgccgaagac gccggacgac ctgccgaacc atgtgtgcct gcgcctcgat acgcccgcgt cgccggccag cgagtggcgg ctcgagcgcg gcgacgacga ggagaccgtc tacgaactgc agccggcgcc gttccaggtc aacgtgccgg atgcgctgtg cgtcgcggtg cgggcggggc gcggcatcgc gtgcgtcgcg ctgtacacgg tgctcgacga ccttcgcgaa gggcggctga tccgcgtgct gcccgagtac cgcctgcaga cggtcagcgt gtacgccgtc tatgcgacgc gccgctatct ggacgcgaag atccgcacct tcctcgacca tctgcgcacg acgctcacgc ccgcgctgga gaacgacctg cgcgaactcg accggctcac ggtcgagcac acgccgggca agcgccagcg cgcgtaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for BuamA.12800.j.A1.PW31695
PURIFICATION DATe: 5/27/2011
CONCENTRATION: 24.62mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 50µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMDMLENM RLFVRVVEAG SFTAVAKEID ATTAQVSRAV SNLEAHVQTR LLHRTTRHLG LTESGERYFE RAKSILAEID YANAEARNAL LRPSGKMRIH AMTGLGQSHV VSSIVRYQED NPDVSVELTL AQRMPNLVEE GYDVSIVTAS QLPDSGYVAQ TCGTSCSVLV ASRDYLARHG TPKTPDDLPN HVCLRLDTPA SPASEWRLER GDDEETVYEL QPAPFQVNVP DALCVAVRAG RGIACVALYT VLDDLREGRL IRVLPEYRLQ TVSVYAVYAT RRYLDAKIRT FLDHLRTTLT PALENDLREL DRLTVEHTPG KRQRA
Validated NT Sequence
ttattacgcg cgctggcgct tgcccggcgt gtgctcgacc gtgagccggt cgagttcgcg caggtcgttc tccagcgcgg gcgtgagcgt cgtgcgcaga tggtcgagga aggtgcggat cttcgcgtcc agatagcggc gcgtcgcata gacggcgtac acgctgaccg tctgcaggcg gtactcgggc agcacgcgga tcagccgccc ttcgcgaagg tcgtcgagca ccgtgtacag cgcgacgcac gcgatgccgc gccccgcccg caccgcgacg cacagcgcat ccggcacgtt gacctggaac ggcgccggct gcagttcgta gacggtctcc tcgtcgtcgc cgcgctcgag ccgccactcg ctggccggcg acgcgggcgt atcgaggcgc aggcacacat ggttcggcag gtcgtccggc gtcttcggcg tgccgtgccg cgcgaggtag tcgcgcgatg cgaccagcac gctgcagctc gtgccgcagg tctgcgcgac gtagccggaa tcgggcaact gcgacgcggt cacgatcgac acgtcgtagc cctcctcgac gaggttcggc atccgctgcg cgagcgtcag ctcgaccgac acgtccgggt tgtcttcctg atagcgcacg atcgacgaca cgacatgact ctgcccgagc cccgtcatcg catggatgcg catcttgccg ctcggccgca gcagcgcgtt gcgcgcttcg gcgttcgcgt agtcgatttc ggcgaggatc gatttcgccc gctcgaaata gcgctcgccg ctttcggtga gaccgagatg gcgcgtcgtg cgatgcagca ggcgtgtctg cacgtgggct tcgagattcg aaaccgcgcg cgacacctgc gcggtggtcg cgtcgatttc tttcgcgacc gcggtgaaac tgccggcttc gacaacgcgc acgaacaggc gcatgttttc gagcatgtcc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg a
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDMLENMRL FVRVVEAGSF TAVAKEIDAT TAQVSRAVSN LEAHVQTRLL HRTTRHLGLT ESGERYFERA KSILAEIDYA NAEARNALLR PSGKMRIHAM TGLGQSHVVS SIVRYQEDNP DVSVELTLAQ RMPNLVEEGY DVSIVTASQL PDSGYVAQTC GTSCSVLVAS RDYLARHGTP KTPDDLPNHV CLRLDTPASP ASEWRLERGD DEETVYELQP APFQVNVPDA LCVAVRAGRG IACVALYTVL DDLREGRLIR VLPEYRLQTV SVYAVYATRR YLDAKIRTFL DHLRTTLTPA LENDLRELDR LTVEHTPGKR QRA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGACATGCTC GAAAACATGC GCCTGTTCGT GCGCGTTGTC GAAGCCGGCA GTTTCACCGC GGTCGCGAAA GAAATCGACG CGACCACCGC GCAGGTGTCG CGCGCGGTTT CGAATCTCGA AGCCCACGTG CAGACACGCC TGCTGCATCG CACGACGCGC CATCTCGGTC TCACCGAAAG CGGCGAGCGC TATTTCGAGC GGGCGAAATC GATCCTCGCC GAAATCGACT ACGCGAACGC CGAAGCGCGC AACGCGCTGC TGCGGCCGAG CGGCAAGATG CGCATCCATG CGATGACGGG GCTCGGGCAG AGTCATGTCG TGTCGTCGAT CGTGCGCTAT CAGGAAGACA ACCCGGACGT GTCGGTCGAG CTGACGCTCG CGCAGCGGAT GCCGAACCTC GTCGAGGAGG GCTACGACGT GTCGATCGTG ACCGCGTCGC AGTTGCCCGA TTCCGGCTAC GTCGCGCAGA CCTGCGGCAC GAGCTGCAGC GTGCTGGTCG CATCGCGCGA CTACCTCGCG CGGCACGGCA CGCCGAAGAC GCCGGACGAC CTGCCGAACC ATGTGTGCCT GCGCCTCGAT ACGCCCGCGT CGCCGGCCAG CGAGTGGCGG CTCGAGCGCG GCGACGACGA GGAGACCGTC TACGAACTGC AGCCGGCGCC GTTCCAGGTC AACGTGCCGG ATGCGCTGTG CGTCGCGGTG CGGGCGGGGC GCGGCATCGC GTGCGTCGCG CTGTACACGG TGCTCGACGA CCTTCGCGAA GGGCGGCTGA TCCGCGTGCT GCCCGAGTAC CGCCTGCAGA CGGTCAGCGT GTACGCCGTC TATGCGACGC GCCGCTATCT GGACGCGAAG ATCCGCACCT TCCTCGACCA TCTGCGCACG ACGCTCACGC CCGCGCTGGA GAACGACCTG CGCGAACTCG ACCGGCTCAC GGTCGAGCAC ACGCCGGGCA AGCGCCAGCG CGCGTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA