BuamA.12800.i

Transcriptional regulator, LysR family

CENTER ID: BuamA.12800.i
ORGANISM: Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.12800.i.A1.GE31787 Full length( BuamA.12800.i ) 1 309
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|398577.6.peg.2469
RefSeq: YP_001809097.1
UniProt: B1YV77

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDKLDQVRIF LQVAEMGSFI KAAHALDVPR ATVSAAVQQL ETALGTRLLH RTTRQVQLTA DGALLLERGR RLLAEADELD RLFRRRDRDV VGRLNVDAPS RIARRVIAPA LPSLFRRHPK LQLSLGSTDR TIDLVQEGVD CAIRVGRLAD SSLVVRPLGR FALINCASPD YLRECGVPEH PDALAHGHWA IGYASPTTGR ELGWEYCADG GQHTLALPSR VIVNNAETYI ASCIAGMGLI QIPRFDVVHL LDSGALVEVM PGHRAAPMDV SAVYPHRRHR SRRLNAFIEW FGELMADALN DTGAAARGD
NT Sequence
ATGGACAAGC TGGACCAGGT CAGAATCTTC CTTCAGGTTG CCGAGATGGG CAGCTTCATC AAGGCCGCGC ATGCGCTCGA CGTGCCGCGC GCGACCGTGT CGGCCGCTGT CCAGCAGCTC GAGACGGCGC TCGGCACGCG CCTGTTGCAC CGCACGACGC GGCAGGTCCA GCTGACCGCC GACGGCGCGC TGCTGCTCGA GCGCGGCCGG CGCCTGCTCG CGGAAGCCGA CGAACTCGAC CGGCTGTTCC GGCGTCGCGA TCGCGACGTG GTCGGGCGGC TGAACGTCGA TGCGCCGAGC CGGATCGCGC GCCGCGTGAT CGCGCCGGCA CTGCCGTCGC TGTTTCGCCG CCACCCGAAG CTGCAACTGT CGCTCGGCTC GACGGACCGC ACGATCGATC TCGTGCAGGA GGGTGTCGAC TGCGCGATCC GGGTCGGACG GCTCGCGGAC AGCAGCCTCG TCGTGCGGCC GCTCGGGCGG TTCGCGCTGA TCAACTGCGC GAGCCCCGAC TACCTGCGCG AATGCGGCGT GCCCGAGCAT CCCGATGCGC TCGCGCACGG ACATTGGGCG ATCGGCTATG CGTCGCCGAC GACCGGGCGC GAACTCGGCT GGGAATACTG CGCGGATGGC GGGCAGCACA CGCTGGCGCT GCCGAGCCGC GTGATCGTCA ACAACGCCGA AACGTATATC GCGAGCTGCA TCGCCGGGAT GGGGCTGATC CAGATTCCGC GCTTCGACGT CGTGCACCTG CTCGACAGCG GTGCGCTGGT CGAGGTGATG CCCGGCCATC GCGCCGCGCC GATGGACGTG TCGGCCGTGT ATCCGCATCG CCGGCACCGG TCGCGGCGGC TCAACGCGTT CATCGAGTGG TTCGGGGAGT TGATGGCGGA TGCGCTGAAC GACACGGGCG CGGCCGCGCG CGGCGAC
Details for BuamA.12800.i.A1.GE31787
HARVESTED ON: 3/8/2011
SEQUENCED ON: 3/14/2011
EXPECTED MW: 36kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMDKLDQV RIFLQVAEMG SFIKAAHALD VPRATVSAAV QQLETALGTR LLHRTTRQVQ LTADGALLLE RGRRLLAEAD ELDRLFRRRD RDVVGRLNVD APSRIARRVI APALPSLFRR HPKLQLSLGS TDRTIDLVQE GVDCAIRVGR LADSSLVVRP LGRFALINCA SPDYLRECGV PEHPDALAHG HWAIGYASPT TGRELGWEYC ADGGQHTLAL PSRVIVNNAE TYIASCIAGM GLIQIPRFDV VHLLDSGALV EVMPGHRAAP MDVSAVYPHR RHRSRRLNAF IEWFGELMAD ALNDTGAAAR GD
Validated NT Sequence
tagtcgccgc gcgcggccgc gcccgtgtcg ttcagcgcat ccgccatcaa ctccccgaac cactcgatga acgcgttgag ccgccgcgac cggtgccggc gatgcggata cacggccgac acgtccatcg gcgcggcgcg atggccgggc atcacctcga ccagcgcacc gctgtcgagc aggtgcacga cgtcgaagcg cggaatctgg atcagcccca tcccggcgat gcagctcgcg atatacgttt cggcgttgtt gacgatcacg cggctcggca gcgccagcgt gtgctgcccg ccatccgcgc agtattccca gccgagttcg cgcccggtcg tcggcgacgc atagccgatc gcccaatgtc cgtgcgcgag cgcatcggga tgctcgggca cgccgcattc gcgcaggtag tcggggctcg cgcagttgat cagcgcgaac cgcccgagcg gccgcacgac gaggctgctg tccgcgagcc gtccgacccg gatcgcgcag tcgacaccct cctgcacgag atcgatcgtg cggtccgtcg agccgagcga cagttgcagc ttcgggtggc ggcgaaacag cgacggcagt gccggcgcga tcacgcggcg cgcgatccgg ctcggcgcat cgacgttcag ccgcccgacc acgtcgcgat cgcgacgccg gaacagccgg tcgagttcgt cggcttccgc gagcaggcgc cggccgcgct cgagcagcag cgcgccgtcg gcggtcagct ggacctgccg cgtcgtgcgg tgcaacaggc gcgtgccgag cgccgtctcg agctgctgga cagcggccga cacggtcgcg cgcggcacgt cgagcgcatg cgcggccttg atgaagctgc ccatctcggc aacctgaagg aagattctga cctggtccag cttgtccatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgang gtg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDKLDQVRI FLQVAEMGSF IKAAHALDVP RATVSAAVQQ LETALGTRLL HRTTRQVQLT ADGALLLERG RRLLAEADEL DRLFRRRDRD VVGRLNVDAP SRIARRVIAP ALPSLFRRHP KLQLSLGSTD RTIDLVQEGV DCAIRVGRLA DSSLVVRPLG RFALINCASP DYLRECGVPE HPDALAHGHW AIGYASPTTG RELGWEYCAD GGQHTLALPS RVIVNNAETY IASCIAGMGL IQIPRFDVVH LLDSGALVEV MPGHRAAPMD VSAVYPHRRH RSRRLNAFIE WFGELMADAL NDTGAAARGD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacaagctg gaccaggtca gaatcttcct tcaggttgcc gagatgggca gcttcatcaa ggccgcgcat gcgctcgacg tgccgcgcgc gaccgtgtcg gccgctgtcc agcagctcga gacggcgctc ggcacgcgcc tgttgcaccg cacgacgcgg caggtccagc tgaccgccga cggcgcgctg ctgctcgagc gcggccggcg cctgctcgcg gaagccgacg aactcgaccg gctgttccgg cgtcgcgatc gcgacgtggt cgggcggctg aacgtcgatg cgccgagccg gatcgcgcgc cgcgtgatcg cgccggcact gccgtcgctg tttcgccgcc acccgaagct gcaactgtcg ctcggctcga cggaccgcac gatcgatctc gtgcaggagg gtgtcgactg cgcgatccgg gtcggacggc tcgcggacag cagcctcgtc gtgcggccgc tcgggcggtt cgcgctgatc aactgcgcga gccccgacta cctgcgcgaa tgcggcgtgc ccgagcatcc cgatgcgctc gcgcacggac attgggcgat cggctatgcg tcgccgacga ccgggcgcga actcggctgg gaatactgcg cggatggcgg gcagcacacg ctggcgctgc cgagccgcgt gatcgtcaac aacgccgaaa cgtatatcgc gagctgcatc gccgggatgg ggctgatcca gattccgcgc ttcgacgtcg tgcacctgct cgacagcggt gcgctggtcg aggtgatgcc cggccatcgc gccgcgccga tggacgtgtc ggccgtgtat ccgcatcgcc ggcaccggtc gcggcggctc aacgcgttca tcgagtggtt cggggagttg atggcggatg cgctgaacga cacgggcgcg gccgcgcgcg gcgactaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa