SSGCID: Target Page

BuamA.12800.h

Transcriptional regulator, LysR family

CENTER ID:	BuamA.12800.h
ORGANISM:	Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuamA.12800.h.A1.GE31786	Full length( BuamA.12800.h )		1	328

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|398577.6.peg.734
RefSeq:	YP_001807435.1
UniProt:	B1YTZ6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNQIQTMRVF VCVAEQQSFR RAAHHLGVSN ALVTRSIAML ESHLNTRLIH RTTRNLSLTE AGLRYLDGCR ALLEEFDHLE ASVAHAVREP AGTLRVVASS LLSPLALTPL VSSFRHRYPE LRVQLTVAEG PLDVLDSGYD VGIVTGNRLD GNPTLIGHAL PSAPFVACAA PAYLERRGEP RAPDDLPGHD WVALAPHQHA PAWRLVGHDG ATHSITVRPT CTVNQLALVH AAAVAGSGIV VLPEPCVADA LATGTLVRLL PAYQIDDPDA QLSLVYPNRQ YVPARTRSFI EHALEHFGVQ AAREPSGYSF LRTPDRVDLV TGRQTGLQ

NT Sequence

ATGAACCAGA TTCAGACCAT GCGTGTGTTC GTCTGCGTCG CCGAGCAGCA GAGCTTCCGC CGCGCGGCGC ACCATCTAGG CGTGTCCAAT GCGCTCGTCA CGCGCTCGAT AGCGATGCTC GAGTCTCACC TGAACACGCG GTTGATCCAC CGCACCACGC GCAACCTGTC GCTGACCGAG GCCGGGCTGC GCTACCTCGA CGGCTGCCGC GCGCTGCTCG AGGAATTCGA TCATCTCGAG GCATCCGTCG CGCATGCGGT GCGCGAGCCG GCCGGCACGC TGCGGGTCGT CGCGTCGAGC CTGCTGTCGC CGCTCGCACT GACGCCGCTC GTCAGCAGCT TCCGGCACCG CTACCCCGAG CTGCGCGTGC AGCTCACCGT CGCCGAAGGG CCGCTCGACG TGCTCGATTC GGGCTACGAC GTCGGCATCG TGACCGGCAA CCGGCTCGAC GGCAACCCGA CGCTGATCGG CCACGCACTC CCGTCGGCCC CGTTCGTCGC CTGTGCGGCG CCCGCGTATC TCGAACGGCG CGGCGAGCCG CGCGCGCCCG ACGACCTGCC CGGCCACGAC TGGGTCGCAC TCGCGCCGCA CCAGCATGCG CCCGCGTGGC GGCTGGTCGG CCACGACGGC GCAACGCATT CGATCACGGT ACGCCCGACC TGCACGGTCA ACCAGCTGGC GCTCGTGCAC GCGGCGGCCG TGGCCGGGTC CGGCATCGTG GTGCTGCCCG AGCCGTGCGT CGCCGACGCG CTCGCCACCG GCACGCTCGT GCGGCTGTTG CCCGCCTACC AGATCGACGA CCCGGACGCG CAGCTGTCGC TCGTCTACCC GAACCGGCAG TACGTGCCGG CCCGCACGCG CAGCTTCATC GAGCACGCGC TCGAGCATTT CGGCGTGCAG GCGGCCCGCG AGCCAAGCGG CTACAGCTTC CTGCGCACGC CCGATCGCGT CGATCTCGTC ACGGGCCGGC AGACGGGCCT GCAG

Details for BuamA.12800.h.A1.GE31786

HARVESTED ON:	3/8/2011
SEQUENCED ON:	3/14/2011
EXPECTED MW:	37kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Validated AA Sequence

Validated NT Sequence

tgcaggcccg tctgccggcc cgtgacgaga tcgacgcgat cgggcgtgcg caggaagctg tagccgcttg gctcgcgggc cgcctgcacg ccgaaatgct cgagcgcgtg ctcgatgaag ctgcgcgtgc gggccggcac gtactgccgg ttcgggtaga cgagcgacag ctgcgcgtcc gggtcgtcga tctggtaggc gggcaacagc cgcacgagcg tgccggtggc gagcgcgtcg gcgacgcacg gctcgggcag caccacgatg ccggacccgg ccacggccgc cgcgtgcacg agcgccagct ggttgaccgt gcaggtcggg cgtaccgtga tcgaatgcgt tgcgccgtcg tggccgacca gccgccacgc gggcgcatgc tggtgcggcg cgagtgcgac ccagtcgtgg ccgggcaggt cgtcgggcgc gcgcggctcg ccgcgccgtt cgagatacgc gggcgccgca caggcgacga acggggccga cgggagtgcg tggccgatca gcgtcgggtt gccgtcgagc cggttgccgg tcacgatgcc gacgtcgtag cccgaatcga gcacgtcgag cggcccttcg gcgacggtga gctgcacgcg cagctcgggg tagcggtgcc ggaagctgct gacgagcggc gtcagtgcga gcggcgacag caggctcgac gcgacgaccc gcagcgtgcc ggccggctcg cgcaccgcat gcgcgacgga tgcctcgaga tgatcgaatt cctcgagcag cgcgcggcag ccgtcgaggt agcgcagccc ggcctcggtc agcgacaggt tgcgcgtggt gcggtggatc aaccgcgtgt tcaggtgaga ctcgagcatc gctatcgagc gcgtgacgag cgcattggac acgcctagat ggtgcgccgc gcggcggaag ctctgctgct cggcgacgca gacgaacaca cgcatggtct gaatctggtt catcgaacca ggacccnggg tntgagct

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMNQIQTMRV FVCVAEQQSF RRAAHHLGVS NALVTRSIAM LESHLNTRLI HRTTRNLSLT EAGLRYLDGC RALLEEFDHL EASVAHAVRE PAGTLRVVAS SLLSPLALTP LVSSFRHRYP ELRVQLTVAE GPLDVLDSGY DVGIVTGNRL DGNPTLIGHA LPSAPFVACA APAYLERRGE PRAPDDLPGH DWVALAPHQH APAWRLVGHD GATHSITVRP TCTVNQLALV HAAAVAGSGI VVLPEPCVAD ALATGTLVRL LPAYQIDDPD AQLSLVYPNR QYVPARTRSF IEHALEHFGV QAAREPSGYS FLRTPDRVDL VTGRQTGLQ

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaccagatt cagaccatgc gtgtgttcgt ctgcgtcgcc gagcagcaga gcttccgccg cgcggcgcac catctaggcg tgtccaatgc gctcgtcacg cgctcgatag cgatgctcga gtctcacctg aacacgcggt tgatccaccg caccacgcgc aacctgtcgc tgaccgaggc cgggctgcgc tacctcgacg gctgccgcgc gctgctcgag gaattcgatc atctcgaggc atccgtcgcg catgcggtgc gcgagccggc cggcacgctg cgggtcgtcg cgtcgagcct gctgtcgccg ctcgcactga cgccgctcgt cagcagcttc cggcaccgct accccgagct gcgcgtgcag ctcaccgtcg ccgaagggcc gctcgacgtg ctcgattcgg gctacgacgt cggcatcgtg accggcaacc ggctcgacgg caacccgacg ctgatcggcc acgcactccc gtcggccccg ttcgtcgcct gtgcggcgcc cgcgtatctc gaacggcgcg gcgagccgcg cgcgcccgac gacctgcccg gccacgactg ggtcgcactc gcgccgcacc agcatgcgcc cgcgtggcgg ctggtcggcc acgacggcgc aacgcattcg atcacggtac gcccgacctg cacggtcaac cagctggcgc tcgtgcacgc ggcggccgtg gccgggtccg gcatcgtggt gctgcccgag ccgtgcgtcg ccgacgcgct cgccaccggc acgctcgtgc ggctgttgcc cgcctaccag atcgacgacc cggacgcgca gctgtcgctc gtctacccga accggcagta cgtgccggcc cgcacgcgca gcttcatcga gcacgcgctc gagcatttcg gcgtgcaggc ggcccgcgag ccaagcggct acagcttcct gcgcacgccc gatcgcgtcg atctcgtcac gggccggcag acgggcctgc agtaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa