BuamA.12800.g

Transcriptional regulator, LysR family

CENTER ID: BuamA.12800.g
ORGANISM: Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.12800.g.A2.GE31785 Mature protein( BuamA.12800.g ) 25 301
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|398577.6.peg.2002
RefSeq: YP_001808650.1
UniProt: B1YSJ8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDRFKQIETF AAVAAKGSLS AAAHAEGVAP AIIGRRLDAL EERLGVKLLV RTTRKLTLTF EGSAFLEDCQ RIINDMQNAE ASVSAGGVKA SGHLRISAPA GFGRRHVAPL VPEFSGAHPD VSVTLDLSDR MVDLVNEGFD CAVRLGELPD SSLVSLKLGE NRRVCVASPA YLARRGTPAT LAELARHNCL ALAANANQQR GWAFQEGDKV VSIRVNGTME CSDGAVLHEW CLAGYGLAWR SWWEVGGDIA AGRLVSVLDA FAAPPIGIHA VFPQRRHLPL RVRLFLDYLK HTYERPGYWG E
NT Sequence
ATGGACCGGT TCAAACAGAT CGAAACGTTC GCCGCGGTCG CGGCGAAGGG CAGCCTGTCG GCGGCCGCGC ACGCGGAAGG CGTCGCGCCG GCGATCATCG GCCGCCGCCT CGACGCGCTC GAGGAGCGGC TCGGCGTCAA GCTGCTGGTG CGTACGACCC GCAAGCTCAC GCTGACCTTC GAGGGTTCGG CTTTCCTCGA GGATTGCCAG CGCATCATCA ACGACATGCA GAACGCGGAG GCGAGCGTGT CCGCCGGCGG CGTGAAGGCG AGCGGCCACC TGCGCATTTC CGCGCCGGCC GGCTTCGGGC GGCGCCACGT TGCGCCCCTC GTGCCCGAAT TCAGCGGCGC GCATCCGGAC GTGTCGGTCA CGCTCGACCT GTCCGACCGG ATGGTCGACC TCGTCAACGA AGGGTTCGAT TGCGCGGTGC GGCTCGGCGA GCTGCCCGAT TCGTCGCTGG TGTCGCTGAA GCTCGGCGAG AACCGGCGCG TGTGCGTCGC GTCTCCGGCG TATCTCGCAC GGCGCGGCAC GCCGGCCACG CTCGCCGAAC TCGCGCGCCA CAACTGCCTC GCGCTGGCCG CGAACGCGAA CCAGCAGCGC GGCTGGGCGT TCCAGGAGGG CGACAAGGTC GTGTCGATCC GCGTGAACGG CACGATGGAA TGCTCGGACG GCGCGGTGCT GCACGAGTGG TGCCTCGCCG GCTACGGCCT GGCATGGCGA TCGTGGTGGG AGGTCGGCGG CGACATCGCG GCCGGCCGCC TCGTCAGCGT GCTCGATGCG TTCGCGGCGC CGCCGATCGG CATCCACGCG GTGTTTCCGC AGCGCCGCCA CCTGCCGCTG CGCGTGCGGC TGTTTCTCGA TTACCTGAAA CATACGTACG AGCGGCCCGG ATATTGGGGC GAG
Details for BuamA.12800.g.A2.GE31785
HARVESTED ON: 3/8/2011
SEQUENCED ON: 3/14/2011
EXPECTED MW: 32kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 14
PERCENT COVERAGE: 108
Validated AA Sequence
MXXXXXXSPS YGYPGSSDPG SWFDGGRRRA GDHRPPPRRA RGAARRQAAG AYDPQAHADL RGFGFPRGLP AHHQRHAERG GERVRRRREG ERPPAHFRAG RLRAAPRCAP RARIQRRASG RVGHARPVRP DGRPRQRRVR LRGAARRAAR FVAGVAEARR EPARVRRVSG VSRTARHAGH ARRTRAPQLP RAGREREPAA RLGVPGGRQG RVDPRERXDG MLGRRXAARV XXRRLXPGMA IXVGGRRRXX GRXXVSVXDA SRRXXXASRV XPQRALXXAX AVLXTEXXYE XXXWXXXXXX XXXAXFSXXX XXXEXXXLXX XXXX
Validated NT Sequence
gnnnnnnnnn nngcnnnnnn ncnatgtnnn nngnnnnnnc ncnntcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcgg aaggcgtcgc gccggcgatc atcggccgcc gcctcgacgc gctcgaggag cggctcggcg tcaagctgct ggtgcgtacg acccgcaagc tcacgctgac cttcgagggt tcggctttcc tcgaggattg ccagcgcatc atcaacgaca tgcagaacgc ggaggcgagc gtgtccgccg gcggcgtgaa ggcgagcggc cacctgcgca tttccgcgcc ggccggcttc gggcggcgcc acgttgcgcc cctcgtgccc gaattcagcg gcgcgcatcc ggacgtgtcg gtcacgctcg acctgtccga ccggatggtc gacctcgtca acgaagggtt cgattgcgcg gtgcggctcg gcgagctgcc cgattcgtcg ctggtgtcgc tgaagctcgg cgagaaccgg cgcgtgtgcg tcgcgtctcc ggcgtatctc gcacggcgcg gcacgccggc cacgctcgcc gaactcgcgc gccacaactg cctcgcgctg gccgcgaacg cgaaccagca gcgcggctgg gcgttccagg agggcgacaa ggtcgtgtcg atccgcgtga acggnacgat ggaatgctcg gacggcgcgn ngctgcacga gtggnnnctc gccggctacn gcctggcatg gcgatcgngg tgggaggncg gcggcgacnt cncggccggn cgcncgtcag cgtgntcgat gcntcncggc gccnccnanc ggcatcacgc gtgnttccnc agcgcgcact gcnntgngcg ngngctgttc tcgntactga acntncntac gagngncnna nntgggnnan nanaanagna cnanagtntg nagccanctt ctcannncng nnnnnannan ccgaanganc tganttgnnn nnncnnnnnn annnnna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAEGVAPAI IGRRLDALEE RLGVKLLVRT TRKLTLTFEG SAFLEDCQRI INDMQNAEAS VSAGGVKASG HLRISAPAGF GRRHVAPLVP EFSGAHPDVS VTLDLSDRMV DLVNEGFDCA VRLGELPDSS LVSLKLGENR RVCVASPAYL ARRGTPATLA ELARHNCLAL AANANQQRGW AFQEGDKVVS IRVNGTMECS DGAVLHEWCL AGYGLAWRSW WEVGGDIAAG RLVSVLDAFA APPIGIHAVF PQRRHLPLRV RLFLDYLKHT YERPGYWGE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcggaaggc gtcgcgccgg cgatcatcgg ccgccgcctc gacgcgctcg aggagcggct cggcgtcaag ctgctggtgc gtacgacccg caagctcacg ctgaccttcg agggttcggc tttcctcgag gattgccagc gcatcatcaa cgacatgcag aacgcggagg cgagcgtgtc cgccggcggc gtgaaggcga gcggccacct gcgcatttcc gcgccggccg gcttcgggcg gcgccacgtt gcgcccctcg tgcccgaatt cagcggcgcg catccggacg tgtcggtcac gctcgacctg tccgaccgga tggtcgacct cgtcaacgaa gggttcgatt gcgcggtgcg gctcggcgag ctgcccgatt cgtcgctggt gtcgctgaag ctcggcgaga accggcgcgt gtgcgtcgcg tctccggcgt atctcgcacg gcgcggcacg ccggccacgc tcgccgaact cgcgcgccac aactgcctcg cgctggccgc gaacgcgaac cagcagcgcg gctgggcgtt ccaggagggc gacaaggtcg tgtcgatccg cgtgaacggc acgatggaat gctcggacgg cgcggtgctg cacgagtggt gcctcgccgg ctacggcctg gcatggcgat cgtggtggga ggtcggcggc gacatcgcgg ccggccgcct cgtcagcgtg ctcgatgcgt tcgcggcgcc gccgatcggc atccacgcgg tgtttccgca gcgccgccac ctgccgctgc gcgtgcggct gtttctcgat tacctgaaac atacgtacga gcggcccgga tattggggcg agtaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa