BuamA.12800.c

Transcriptional regulator, LysR family

CENTER ID: BuamA.12800.c
ORGANISM: Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.12800.c.A1.GE31781 Full length( BuamA.12800.c ) 1 349
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.12800.c.A1.PS01075 Full length( BuamA.12800.c ) 1 349

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|398577.6.peg.3178
RefSeq: YP_001809761.1
UniProt: B1YPY1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDHLQSMRVF VKVADLGSFA RAASAMDISN AVATRHVADL EGRLGTRLLN RTTRSLSLTE SGQVYLERAR QILDELEDVE QMVVARNHEP VGTLRIVAPV VFGLHNLAPV LQSYTENFPK VVPDLTLVDR QVDLVEEGFD VGIVVTRQMR SASIVTRRLT TGCMTVCATP SYLEKHGVPT HPEQLAEHPA LSLPTEYWGD ERVFTGPDGE VRVRPTNVIV ANNTAMLRQF ALLGMGVAIL PSYLIGGDIA RGALVRLLPD FRLPQVEINI AYPSRRHLPA KVRTFIDHLV EYFSHATDAV IGEQWAAQSA MPAPIGVEMR AEQPESTDPN LQPRVARSAR PRVAVPSPL
NT Sequence
ATGGATCATT TGCAGTCGAT GCGCGTGTTC GTCAAGGTTG CGGATCTCGG CAGTTTCGCG CGGGCCGCGA GTGCTATGGA TATCTCCAAC GCGGTCGCGA CACGTCACGT CGCCGATCTG GAGGGCCGCC TCGGCACACG GTTGCTGAAC CGCACCACCC GCAGCCTTTC GCTGACGGAG TCGGGCCAGG TCTATCTGGA GCGGGCGCGC CAGATTCTCG ATGAGCTCGA GGATGTCGAA CAGATGGTCG TCGCGCGCAA TCACGAGCCG GTCGGCACGT TGCGCATCGT CGCGCCGGTC GTGTTCGGCC TGCATAACCT TGCGCCCGTC CTGCAGTCGT ACACGGAGAA TTTCCCGAAA GTCGTGCCGG ATCTGACGCT GGTCGACCGC CAGGTCGATC TCGTCGAGGA AGGTTTCGAC GTCGGCATCG TCGTCACGCG CCAGATGCGC AGCGCGAGCA TCGTCACGCG GCGCCTGACC ACGGGCTGCA TGACCGTGTG CGCGACGCCG AGCTACCTGG AAAAGCATGG CGTGCCGACT CATCCGGAAC AACTCGCCGA GCATCCGGCG CTGAGCCTGC CGACCGAATA CTGGGGCGAC GAGCGCGTGT TCACGGGGCC CGACGGTGAA GTGCGCGTGC GCCCGACCAA CGTGATCGTC GCCAATAACA CCGCGATGCT GCGTCAGTTC GCGCTGCTCG GGATGGGCGT CGCGATCCTG CCGAGCTACC TGATCGGCGG CGACATCGCG CGCGGCGCGC TGGTGCGGTT GCTGCCGGAT TTCCGGCTGC CGCAGGTCGA GATCAACATC GCATACCCGA GCCGGCGCCA TCTGCCCGCG AAGGTGCGCA CGTTCATCGA CCACCTCGTC GAATATTTCA GCCACGCGAC CGACGCGGTG ATTGGCGAAC AGTGGGCCGC ACAGAGTGCG ATGCCGGCGC CGATCGGCGT CGAGATGCGT GCCGAGCAGC CCGAATCGAC CGACCCGAAC CTGCAGCCGC GCGTCGCGCG TTCGGCGCGC CCGCGCGTCG CGGTGCCGTC ACCGCTG
Details for BuamA.12800.c.A1.GE31781
HARVESTED ON: 3/8/2011
SEQUENCED ON: 3/14/2011
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MDHLQSMRVF VKVADLGSFA RAASAMDISN AVATRHVADL EGRLGTRLLN RTTRSLSLTE SGQVYLERAR QILDELEDVE QMVVARNHEP VGTLRIVAPV VFGLHNLAPV LQSYTENFPK VVPDLTLVDR QVDLVEEGFD VGIVVTRQMR SASIVTRRLT TGCMTVCATP SYLEKHGVPT HPEQLAEHPA LSLPTEYWGD ERVFTGPDGE VRVRPTNVIV ANNTAMLRQF ALLGMGVAIL PSYLIGGDIA RGALVRLLPD FRLPQVEINI AYPSRRHLPA KVRTFIDHLV EYFSHATDAV IGEQWAAQSA MPAPIGVEMR AEQPESTDPN LQPRVARSAR PRVAVPSP
Validated NT Sequence
agcggtgacg gcaccgcgac gcgcgggcgc gccgaacgcg cgacgcgcgg ctgcaggttc gggtcggtcg attcgggctg ctcggcacgc atctcgacgc cgatcggcgc cggcatcgca ctctgtgcgg cccactgttc gccaatcacc gcgtcggtcg cgtggctgaa atattcgacg aggtggtcga tgaacgtgcg caccttcgcg ggcagatggc gccggctcgg gtatgcgatg ttgatctcga cctgcggcag ccggaaatcc ggcagcaacc gcaccagcgc gccgcgcgcg atgtcgccgc cgatcaggta gctcggcagg atcgcgacgc ccatcccgag cagcgcgaac tgacgcagca tcgcggtgtt attggcgacg atcacgttgg tcgggcgcac gcgcacttca ccgtcgggcc ccgtgaacac gcgctcgtcg ccccagtatt cggtcggcag gctcagcgcc ggatgctcgg cgagttgttc cggatgagtc ggcacgccat gcttttccag gtagctcggc gtcgcgcaca cggtcatgca gcccgtggtc aggcgccgcg tgacgatgct cgcgctgcgc atctggcgcg tgacgacgat gccgacgtcg aaaccttcct cgacgagatc gacctggcgg tcgaccagcg tcagatccgg cacgactttc gggaaattct ccgtgtacga ctgcaggacg ggcgcaaggt tatgcaggcc gaacacgacc ggcgcgacga tgcgcaacgt gccgaccggc tcgtgattgc gcgcgacgac catctgttcg acatcctcga gctcatcgag aatctggcgc gcccgctcca gatagacctg gcccgactcc gtcagcgaaa ggctgcgggt ggtgcggttc agcaaccgtg tgccgaggcg gccctccaga tcggcgacgt gacgtgtcgc gaccgcgttg gagatatcca tagcactcgc ggcccgcgcg aaactgccga gatccgcaac cttgacgaac acgcgcatcg actgcaaatg atccatcgaa ccaggaccct gggtctgagc ttcca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDHLQSMRV FVKVADLGSF ARAASAMDIS NAVATRHVAD LEGRLGTRLL NRTTRSLSLT ESGQVYLERA RQILDELEDV EQMVVARNHE PVGTLRIVAP VVFGLHNLAP VLQSYTENFP KVVPDLTLVD RQVDLVEEGF DVGIVVTRQM RSASIVTRRL TTGCMTVCAT PSYLEKHGVP THPEQLAEHP ALSLPTEYWG DERVFTGPDG EVRVRPTNVI VANNTAMLRQ FALLGMGVAI LPSYLIGGDI ARGALVRLLP DFRLPQVEIN IAYPSRRHLP AKVRTFIDHL VEYFSHATDA VIGEQWAAQS AMPAPIGVEM RAEQPESTDP NLQPRVARSA RPRVAVPSPL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggatcatttg cagtcgatgc gcgtgttcgt caaggttgcg gatctcggca gtttcgcgcg ggccgcgagt gctatggata tctccaacgc ggtcgcgaca cgtcacgtcg ccgatctgga gggccgcctc ggcacacggt tgctgaaccg caccacccgc agcctttcgc tgacggagtc gggccaggtc tatctggagc gggcgcgcca gattctcgat gagctcgagg atgtcgaaca gatggtcgtc gcgcgcaatc acgagccggt cggcacgttg cgcatcgtcg cgccggtcgt gttcggcctg cataaccttg cgcccgtcct gcagtcgtac acggagaatt tcccgaaagt cgtgccggat ctgacgctgg tcgaccgcca ggtcgatctc gtcgaggaag gtttcgacgt cggcatcgtc gtcacgcgcc agatgcgcag cgcgagcatc gtcacgcggc gcctgaccac gggctgcatg accgtgtgcg cgacgccgag ctacctggaa aagcatggcg tgccgactca tccggaacaa ctcgccgagc atccggcgct gagcctgccg accgaatact ggggcgacga gcgcgtgttc acggggcccg acggtgaagt gcgcgtgcgc ccgaccaacg tgatcgtcgc caataacacc gcgatgctgc gtcagttcgc gctgctcggg atgggcgtcg cgatcctgcc gagctacctg atcggcggcg acatcgcgcg cggcgcgctg gtgcggttgc tgccggattt ccggctgccg caggtcgaga tcaacatcgc atacccgagc cggcgccatc tgcccgcgaa ggtgcgcacg ttcatcgacc acctcgtcga atatttcagc cacgcgaccg acgcggtgat tggcgaacag tgggccgcac agagtgcgat gccggcgccg atcggcgtcg agatgcgtgc cgagcagccc gaatcgaccg acccgaacct gcagccgcgc gtcgcgcgtt cggcgcgccc gcgcgtcgcg gtgccgtcac cgctgtaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for BuamA.12800.c.A1.PS01075
PURIFICATION DATe: 5/9/2011
CONCENTRATION: 18.78mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Protocol Notes
notes unavailable
Validated AA Sequence
MDHLQSMRVF VKVADLGSFA RAASAMDISN AVATRHVADL EGRLGTRLLN RTTRSLSLTE SGQVYLERAR QILDELEDVE QMVVARNHEP VGTLRIVAPV VFGLHNLAPV LQSYTENFPK VVPDLTLVDR QVDLVEEGFD VGIVVTRQMR SASIVTRRLT TGCMTVCATP SYLEKHGVPT HPEQLAEHPA LSLPTEYWGD ERVFTGPDGE VRVRPTNVIV ANNTAMLRQF ALLGMGVAIL PSYLIGGDIA RGALVRLLPD FRLPQVEINI AYPSRRHLPA KVRTFIDHLV EYFSHATDAV IGEQWAAQSA MPAPIGVEMR AEQPESTDPN LQPRVARSAR PRVAVPSP
Validated NT Sequence
agcggtgacg gcaccgcgac gcgcgggcgc gccgaacgcg cgacgcgcgg ctgcaggttc gggtcggtcg attcgggctg ctcggcacgc atctcgacgc cgatcggcgc cggcatcgca ctctgtgcgg cccactgttc gccaatcacc gcgtcggtcg cgtggctgaa atattcgacg aggtggtcga tgaacgtgcg caccttcgcg ggcagatggc gccggctcgg gtatgcgatg ttgatctcga cctgcggcag ccggaaatcc ggcagcaacc gcaccagcgc gccgcgcgcg atgtcgccgc cgatcaggta gctcggcagg atcgcgacgc ccatcccgag cagcgcgaac tgacgcagca tcgcggtgtt attggcgacg atcacgttgg tcgggcgcac gcgcacttca ccgtcgggcc ccgtgaacac gcgctcgtcg ccccagtatt cggtcggcag gctcagcgcc ggatgctcgg cgagttgttc cggatgagtc ggcacgccat gcttttccag gtagctcggc gtcgcgcaca cggtcatgca gcccgtggtc aggcgccgcg tgacgatgct cgcgctgcgc atctggcgcg tgacgacgat gccgacgtcg aaaccttcct cgacgagatc gacctggcgg tcgaccagcg tcagatccgg cacgactttc gggaaattct ccgtgtacga ctgcaggacg ggcgcaaggt tatgcaggcc gaacacgacc ggcgcgacga tgcgcaacgt gccgaccggc tcgtgattgc gcgcgacgac catctgttcg acatcctcga gctcatcgag aatctggcgc gcccgctcca gatagacctg gcccgactcc gtcagcgaaa ggctgcgggt ggtgcggttc agcaaccgtg tgccgaggcg gccctccaga tcggcgacgt gacgtgtcgc gaccgcgttg gagatatcca tagcactcgc ggcccgcgcg aaactgccga gatccgcaac cttgacgaac acgcgcatcg actgcaaatg atccatcgaa ccaggaccct gggtctgagc ttcca
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDHLQSMRV FVKVADLGSF ARAASAMDIS NAVATRHVAD LEGRLGTRLL NRTTRSLSLT ESGQVYLERA RQILDELEDV EQMVVARNHE PVGTLRIVAP VVFGLHNLAP VLQSYTENFP KVVPDLTLVD RQVDLVEEGF DVGIVVTRQM RSASIVTRRL TTGCMTVCAT PSYLEKHGVP THPEQLAEHP ALSLPTEYWG DERVFTGPDG EVRVRPTNVI VANNTAMLRQ FALLGMGVAI LPSYLIGGDI ARGALVRLLP DFRLPQVEIN IAYPSRRHLP AKVRTFIDHL VEYFSHATDA VIGEQWAAQS AMPAPIGVEM RAEQPESTDP NLQPRVARSA RPRVAVPSPL
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGATCATTTG CAGTCGATGC GCGTGTTCGT CAAGGTTGCG GATCTCGGCA GTTTCGCGCG GGCCGCGAGT GCTATGGATA TCTCCAACGC GGTCGCGACA CGTCACGTCG CCGATCTGGA GGGCCGCCTC GGCACACGGT TGCTGAACCG CACCACCCGC AGCCTTTCGC TGACGGAGTC GGGCCAGGTC TATCTGGAGC GGGCGCGCCA GATTCTCGAT GAGCTCGAGG ATGTCGAACA GATGGTCGTC GCGCGCAATC ACGAGCCGGT CGGCACGTTG CGCATCGTCG CGCCGGTCGT GTTCGGCCTG CATAACCTTG CGCCCGTCCT GCAGTCGTAC ACGGAGAATT TCCCGAAAGT CGTGCCGGAT CTGACGCTGG TCGACCGCCA GGTCGATCTC GTCGAGGAAG GTTTCGACGT CGGCATCGTC GTCACGCGCC AGATGCGCAG CGCGAGCATC GTCACGCGGC GCCTGACCAC GGGCTGCATG ACCGTGTGCG CGACGCCGAG CTACCTGGAA AAGCATGGCG TGCCGACTCA TCCGGAACAA CTCGCCGAGC ATCCGGCGCT GAGCCTGCCG ACCGAATACT GGGGCGACGA GCGCGTGTTC ACGGGGCCCG ACGGTGAAGT GCGCGTGCGC CCGACCAACG TGATCGTCGC CAATAACACC GCGATGCTGC GTCAGTTCGC GCTGCTCGGG ATGGGCGTCG CGATCCTGCC GAGCTACCTG ATCGGCGGCG ACATCGCGCG CGGCGCGCTG GTGCGGTTGC TGCCGGATTT CCGGCTGCCG CAGGTCGAGA TCAACATCGC ATACCCGAGC CGGCGCCATC TGCCCGCGAA GGTGCGCACG TTCATCGACC ACCTCGTCGA ATATTTCAGC CACGCGACCG ACGCGGTGAT TGGCGAACAG TGGGCCGCAC AGAGTGCGAT GCCGGCGCCG ATCGGCGTCG AGATGCGTGC CGAGCAGCCC GAATCGACCG ACCCGAACCT GCAGCCGCGC GTCGCGCGTT CGGCGCGCCC GCGCGTCGCG GTGCCGTCAC CGCTGTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA