SSGCID: Target Page

BuamA.12800.a

Transcriptional regulator, LysR family

CENTER ID:	BuamA.12800.a
ORGANISM:	Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuamA.12800.a.A1.GE31779	Full length( BuamA.12800.a )		1	307

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|398577.6.peg.1335
RefSeq:	YP_001808011.1
UniProt:	B1YNH7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQHLPDLEAW AIFARVAETG SFAKAAELLG ISQPTVSKAI ARLEKRLGAM LLYRTSRKLS LTPTGELLRD RAIRLLADAE LIESEACAQA LEPHGLVRVN APMSFGLRHL SPLLPAFLER YPRVDIDLVL TDHIVDIVSG GFDVAIRIAE LSDSSLRSRR LCAVRRPLVA SPAYLDRHGR PTHPRDIEQH VCLTYTNLPT PEHWRFRNPA SGEDVGVSVH GRIRTNNADV IAPALLAGHG LALQPEFLAW DALQSGELEE VMPEWKIADV NVNLVTPPGL LRAARVTVLL DFLADHFAHA PWALPVA

NT Sequence

ATGCAACATC TTCCTGATCT CGAAGCCTGG GCCATCTTCG CGCGCGTCGC GGAGACCGGT TCGTTCGCGA AAGCCGCGGA ACTGCTCGGC ATCTCGCAAC CGACCGTGTC GAAGGCCATC GCGCGCCTCG AAAAGCGCCT CGGCGCGATG CTGCTGTACC GCACGTCGCG CAAACTGTCG CTCACACCGA CCGGCGAGCT GCTGCGCGAC CGCGCGATCC GCCTGCTCGC CGATGCGGAA CTGATCGAGA GCGAGGCCTG CGCGCAAGCG CTCGAACCGC ACGGTCTCGT GCGCGTGAAC GCGCCGATGT CGTTCGGGCT GCGCCATCTG TCGCCGCTGC TGCCCGCGTT TCTCGAGCGC TATCCGCGCG TCGACATCGA CCTCGTGCTG ACCGACCACA TCGTCGACAT CGTGTCCGGC GGGTTCGACG TCGCGATCCG CATCGCCGAG CTGAGCGATT CATCGCTGCG CTCGCGCCGC CTGTGCGCGG TGCGCCGTCC GCTCGTCGCA TCGCCCGCAT ATCTCGACCG GCATGGCCGT CCCACGCATC CCCGCGACAT CGAACAGCAC GTGTGCCTCA CCTACACGAA CCTGCCGACG CCCGAGCACT GGCGCTTTCG CAATCCGGCT TCGGGCGAGG ATGTCGGCGT GTCCGTGCAC GGACGCATCC GCACCAACAA TGCCGACGTG ATCGCCCCCG CGCTCCTCGC CGGTCACGGC CTCGCGCTGC AGCCCGAGTT CCTCGCGTGG GACGCGCTGC AGAGCGGCGA GCTCGAGGAA GTGATGCCCG AGTGGAAAAT CGCCGATGTC AACGTCAATC TCGTCACGCC GCCCGGGCTG CTGCGCGCCG CACGCGTGAC GGTGCTGCTC GATTTCCTCG CCGATCACTT CGCGCACGCG CCGTGGGCGT TGCCCGTGGC C

Details for BuamA.12800.a.A1.GE31779

HARVESTED ON:	3/8/2011
SEQUENCED ON:	3/14/2011
EXPECTED MW:	36kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	95

Validated AA Sequence

MGTLEAQTQG PGSMQHLPDL EAWAIFARVA ETGSFAKAAE LLGISQPTVS KAIARLEKRL GAMLLYRTSR KLSLTPTGEL LRDRAIRLLA DAELIESEAC AQALEPHGLV RVNAPMSFGL RHLSPLLPAF LERYPRVDID LVLTDHIVDI VSGGFDVAIR IAELSDSSLR SRRLCAVRRP LVASPAYLDR HGRPTHPRDI EQHVCLTYTN LPTPEHWRFR NPASGEDVGV SVHGRIRTNN ADVIAPALLA GHGLALQPEF LAWDALQSGE LEEVMPEWKI ADVNVNLVTP PGLLRAARVT VLLDFLADHF AHA

Validated NT Sequence

gcgcgtgcgc gaagtgatcg gcgaggaaat cgagcagcac cgtcacgcgt gcggcgcgca gcagcccggg cggcgtgacg agattgacgt tgacatcggc gattttccac tcgggcatca cttcctcgag ctcgccgctc tgcagcgcgt cccacgcgag gaactcgggc tgcagcgcga ggccgtgacc ggcgaggagc gcgggggcga tcacgtcggc attgttggtg cggatgcgtc cgtgcacgga cacgccgaca tcctcgcccg aagccggatt gcgaaagcgc cagtgctcgg gcgtcggcag gttcgtgtag gtgaggcaca cgtgctgttc gatgtcgcgg ggatgcgtgg gacggccatg ccggtcgaga tatgcgggcg atgcgacgag cggacggcgc accgcgcaca ggcggcgcga gcgcagcgat gaatcgctca gctcggcgat gcggatcgcg acgtcgaacc cgccggacac gatgtcgacg atgtggtcgg tcagcacgag gtcgatgtcg acgcgcggat agcgctcgag aaacgcgggc agcagcggcg acagatggcg cagcccgaac gacatcggcg cgttcacgcg cacgagaccg tgcggttcga gcgcttgcgc gcaggcctcg ctctcgatca gttccgcatc ggcgagcagg cggatcgcgc ggtcgcgcag cagctcgccg gtcggtgtga gcgacagttt gcgcgacgtg cggtacagca gcatcgcgcc gaggcgcttt tcgaggcgcg cgatggcctt cgacacggtc ggttgcgaga tgccgagcag ttccgcggct ttcgcgaacg aaccggtctc cgcgacgcgc gcgaagatgg cccaggcttc gagatcagga agatgttgca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMQHLPDLEA WAIFARVAET GSFAKAAELL GISQPTVSKA IARLEKRLGA MLLYRTSRKL SLTPTGELLR DRAIRLLADA ELIESEACAQ ALEPHGLVRV NAPMSFGLRH LSPLLPAFLE RYPRVDIDLV LTDHIVDIVS GGFDVAIRIA ELSDSSLRSR RLCAVRRPLV ASPAYLDRHG RPTHPRDIEQ HVCLTYTNLP TPEHWRFRNP ASGEDVGVSV HGRIRTNNAD VIAPALLAGH GLALQPEFLA WDALQSGELE EVMPEWKIAD VNVNLVTPPG LLRAARVTVL LDFLADHFAH APWALPVA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcaacatctt cctgatctcg aagcctgggc catcttcgcg cgcgtcgcgg agaccggttc gttcgcgaaa gccgcggaac tgctcggcat ctcgcaaccg accgtgtcga aggccatcgc gcgcctcgaa aagcgcctcg gcgcgatgct gctgtaccgc acgtcgcgca aactgtcgct cacaccgacc ggcgagctgc tgcgcgaccg cgcgatccgc ctgctcgccg atgcggaact gatcgagagc gaggcctgcg cgcaagcgct cgaaccgcac ggtctcgtgc gcgtgaacgc gccgatgtcg ttcgggctgc gccatctgtc gccgctgctg cccgcgtttc tcgagcgcta tccgcgcgtc gacatcgacc tcgtgctgac cgaccacatc gtcgacatcg tgtccggcgg gttcgacgtc gcgatccgca tcgccgagct gagcgattca tcgctgcgct cgcgccgcct gtgcgcggtg cgccgtccgc tcgtcgcatc gcccgcatat ctcgaccggc atggccgtcc cacgcatccc cgcgacatcg aacagcacgt gtgcctcacc tacacgaacc tgccgacgcc cgagcactgg cgctttcgca atccggcttc gggcgaggat gtcggcgtgt ccgtgcacgg acgcatccgc accaacaatg ccgacgtgat cgcccccgcg ctcctcgccg gtcacggcct cgcgctgcag cccgagttcc tcgcgtggga cgcgctgcag agcggcgagc tcgaggaagt gatgcccgag tggaaaatcg ccgatgtcaa cgtcaatctc gtcacgccgc ccgggctgct gcgcgccgca cgcgtgacgg tgctgctcga tttcctcgcc gatcacttcg cgcacgcgcc gtgggcgttg cccgtggcct aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa