BuamA.12746.a

Beta-lactamase domain protein

CENTER ID:	BuamA.12746.a
ORGANISM:	Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuamA.12746.a.B1.GE39261	Full length( BuamA.12746.a )		1	339

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_140167
PATRIC ID:	fig\|398577.6.peg.4334
RefSeq:	YP_001810877.1
UniProt:	B1Z381

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQVDDPLTPL NTWLEATERN MAKAFASQAD LEVKKITWTQ LSENAYAYTA EGDPNSGVII GDDSVLVVDT TATPAMAQDL IAKIRSVTDK PIKHVVLSHY HAVRVLGASA YFAEGAQHVI ASRGTYEMIV ERGEADMKSE IERFPRLFAG VETVPGLTWP TLVFEREITL FLGKLEVKIM HVGSGHTKGD TIVWLPSQKV LFSGDLVEYD AACYCGDAQL EQWPTTLEAL RALGAEKLVP GRGPALLNPA DVNKGLDYTK DFVTTLLAQG RKAVERKLDL KASMALTREA MDPKFGNVFI YEHCLPFDVS RAFDEASGIT HPRIWTAQRD KEMWDALQD

NT Sequence

ATGCAGGTCG ATGATCCACT CACTCCCCTC AACACATGGC TCGAAGCCAC GGAACGCAAC ATGGCCAAAG CATTCGCATC GCAAGCCGAT CTGGAAGTGA AGAAGATCAC CTGGACGCAG CTGTCCGAGA ACGCATATGC CTATACCGCC GAAGGCGACC CGAACTCGGG CGTAATCATC GGCGACGACA GCGTGCTGGT CGTCGATACG ACCGCGACGC CCGCGATGGC GCAAGACCTG ATCGCGAAGA TCCGCAGCGT GACCGACAAG CCGATCAAGC ACGTCGTGCT GTCGCATTAC CACGCGGTGC GCGTGCTCGG CGCGTCCGCG TATTTCGCCG AAGGCGCGCA GCACGTGATC GCGAGCCGCG GCACCTATGA AATGATCGTC GAGCGCGGCG AGGCCGACAT GAAGTCGGAG ATCGAGCGCT TCCCGCGCCT GTTCGCGGGC GTCGAGACGG TGCCGGGCCT GACCTGGCCG ACGCTCGTGT TCGAGCGCGA GATCACGCTG TTCCTCGGCA AGCTCGAAGT GAAGATCATG CACGTCGGCT CGGGCCATAC GAAGGGCGAC ACGATCGTCT GGCTGCCGTC GCAGAAGGTG CTGTTCTCCG GCGACCTGGT CGAATACGAC GCGGCCTGCT ATTGCGGCGA CGCGCAGCTC GAACAATGGC CGACCACGCT CGAGGCGCTG CGCGCGCTCG GCGCGGAAAA GCTCGTGCCG GGCCGCGGTC CCGCGCTGCT GAATCCGGCC GACGTGAACA AGGGTCTCGA CTATACGAAG GACTTCGTGA CGACGCTGCT CGCGCAGGGC CGCAAGGCCG TCGAGCGCAA GCTCGACCTG AAGGCGTCGA TGGCGCTCAC GCGCGAGGCG ATGGATCCGA AGTTCGGCAA CGTGTTCATC TACGAGCACT GTCTGCCGTT CGACGTGTCG CGCGCATTCG ACGAGGCGAG CGGCATCACG CATCCGCGCA TCTGGACCGC GCAGCGCGAC AAGGAAATGT GGGACGCGCT GCAGGAC

Details for BuamA.12746.a.B1.GE39261

HARVESTED ON:	6/10/2015
SEQUENCED ON:	6/5/2015
EXPECTED MW:	39kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMQ VDDPLTPLNT WLEATERNMA KAFASQADLE VKKITWTQLS ENAYAYTAEG DPNSGVIIGD DSVLVVDTTA TPAMAQDLIA KIRSVTDKPI KHVVLSHYHA VRVLGASAYF AEGAQHVIAS RGTYEMIVER GEADMKSEIE RFPRLFAGVE TVPGLTWPTL VFEREITLFL GKLEVKIMHV GSGHTKGDTI VWLPSQKVLF SGDLVEYDAA CYCGDAQLEQ WPTTLEALRA LGAEKLVPGR GPALLNPADV NKGLDYTKDF VTTLLAQGRK AVERKLDLKA SMALTREAMD PKFGNVFIYE HCLPFDVSRA FDEASGITHP RIWTAQRDK

Validated NT Sequence

ttccttgtcg cgctgcgcgg tccagatgcg cggatgcgtg atgccgctcg cctcgtcgaa tgcgcgcgac acgtcgaacg gcagacagtg ctcgtagatg aacacgttgc cgaacttcgg atccatcgcc tcgcgcgtga gcgccatcga cgccttcagg tcgagcttgc gctcgacggc cttgcggccc tgcgcgagca gcgtcgtcac gaagtccttc gtatagtcga gacccttgtt cacgtcggcc ggattcagca gcgcgggacc gcggcccggc acgagctttt ccgcgccgag cgcgcgcagc gcctcgagcg tggtcggcca ttgttcgagc tgcgcgtcgc cgcaatagca ggccgcgtcg tattcgacca ggtcgccgga gaacagcacc ttctgcgacg gcagccagac gatcgtgtcg cccttcgtat ggcccgagcc gacgtgcatg atcttcactt cgagcttgcc gaggaacagc gtgatctcgc gctcgaacac gagcgtcggc caggtcaggc ccggcaccgt ctcgacgccc gcgaacaggc gcgggaagcg ctcgatctcc gacttcatgt cggcctcgcc gcgctcgacg atcatttcat aggtgccgcg gctcgcgatc acgtgctgcg cgccttcggc gaaatacgcg gacgcgccga gcacgcgcac cgcgtggtaa tgcgacagca cgacgtgctt gatcggcttg tcggtcacgc tgcggatctt cgcgatcagg tcttgcgcca tcgcgggcgt cgcggtcgta tcgacgacca gcacgctgtc gtcgccgatg attacgcccg agttcgggtc gccttcggcg gtataggcat atgcgttctc ggacagctgc gtccaggtga tcttcttcac ttccagatcg gcttgcgatg cgaatgcttt ggccatgttg cgttccgtgg cttcgagcca tgtgttgagg ggagtgagtg gatcatcgac ctgcatatgg tggtggtggt ggtgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcagg tcgatgatcc actcactccc ctcaacacat ggctcgaagc cacggaacgc aacatggcca aagcattcgc atcgcaagcc gatctggaag tgaagaagat cacctggacg cagctgtccg agaacgcata tgcctatacc gccgaaggcg acccgaactc gggcgtaatc atcggcgacg acagcgtgct ggtcgtcgat acgaccgcga cgcccgcgat ggcgcaagac ctgatcgcga agatccgcag cgtgaccgac aagccgatca agcacgtcgt gctgtcgcat taccacgcgg tgcgcgtgct cggcgcgtcc gcgtatttcg ccgaaggcgc gcagcacgtg atcgcgagcc gcggcaccta tgaaatgatc gtcgagcgcg gcgaggccga catgaagtcg gagatcgagc gcttcccgcg cctgttcgcg ggcgtcgaga cggtgccggg cctgacctgg ccgacgctcg tgttcgagcg cgagatcacg ctgttcctcg gcaagctcga agtgaagatc atgcacgtcg gctcgggcca tacgaagggc gacacgatcg tctggctgcc gtcgcagaag gtgctgttct ccggcgacct ggtcgaatac gacgcggcct gctattgcgg cgacgcgcag ctcgaacaat ggccgaccac gctcgaggcg ctgcgcgcgc tcggcgcgga aaagctcgtg ccgggccgcg gtcccgcgct gctgaatccg gccgacgtga acaagggtct cgactatacg aaggacttcg tgacgacgct gctcgcgcag ggccgcaagg ccgtcgagcg caagctcgac ctgaaggcgt cgatggcgct cacgcgcgag gcgatggatc cgaagttcgg caacgtgttc atctacgagc actgtctgcc gttcgacgtg tcgcgcgcat tcgacgaggc gagcggcatc acgcatccgc gcatctggac cgcgcagcgc gacaaggaaa tgtgggacgc gctgcaggac tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat