BuamA.12245.d

Amidase, hydantoinase/carbamoylase family

CENTER ID: BuamA.12245.d
ORGANISM: Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.12245.d.B1.GE39807 Full length( BuamA.12245.d ) 1 426
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.12245.d.B1.PS37908 Full length( BuamA.12245.d ) 1 426

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|398577.6.peg.6178
RefSeq: YP_001815718.1
UniProt: B1Z5W9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDTVSETVKR AAFDTSIKVD GRRLWDSLME VAKIGATPKG GVCRLALTDL DKAGRDLIVG WAKAAGCTVT VDTMGNVFMR RAGRVADAAP VVTGSHADSQ PTGGRFDGIY GVLGGLEVIR SLNDHGIETE HPIEVVIWTN EEGSRFAPAM VASGVFAGVF SLEYGLSRKD VDGKTIGEEL ARIGYAGDVP CGGRKLHAAF ELHIEQGPIL EAECKTIGVV TDAQGQRWYE ITFTGQEAHA GPTPMPRRRD ALLGASRVVD LVNRIGLDHA PFGCATVGMM QIHPNSRNVI PGRVFFTVDF RHPDDAVLAK MDAALRDGVA RIAGEIGLET ALEQIFYYKP VAFDPACVAA VRDAADRFGY SHRDIVSGAG HDACYLAQVA PTSMVFVPCV DGISHNEIED ATPAWIEAGA NVLLHAMLSR ACEPAS
NT Sequence
ATGGACACGG TATCGGAAAC AGTGAAGCGG GCAGCATTCG ACACGTCGAT CAAGGTCGAC GGCAGACGGT TGTGGGACAG CCTGATGGAG GTGGCGAAGA TCGGCGCGAC GCCGAAGGGC GGCGTCTGCC GCCTCGCGCT GACCGATCTC GACAAGGCCG GCCGCGACCT GATCGTCGGC TGGGCGAAGG CGGCGGGCTG CACGGTGACG GTCGATACGA TGGGCAACGT ATTCATGCGT CGCGCGGGGC GCGTGGCCGA CGCGGCGCCG GTCGTCACCG GCTCGCACGC GGATTCGCAG CCGACGGGCG GCCGCTTCGA CGGCATCTAC GGCGTGCTCG GCGGCCTCGA GGTGATTCGC AGTCTGAACG ATCACGGCAT CGAGACCGAG CATCCGATCG AGGTCGTGAT CTGGACCAAC GAGGAAGGCT CGCGCTTCGC ACCGGCGATG GTCGCGTCGG GCGTGTTCGC GGGCGTGTTT TCGCTCGAAT ACGGGCTGTC GCGCAAGGAC GTCGACGGCA AGACGATCGG CGAGGAACTG GCGCGCATCG GCTATGCCGG CGACGTGCCG TGCGGCGGCC GCAAGCTGCA TGCGGCGTTC GAGCTGCATA TCGAACAGGG GCCGATTCTC GAAGCGGAGT GCAAGACGAT CGGCGTGGTG ACCGACGCGC AGGGGCAGCG CTGGTATGAG ATCACGTTCA CCGGCCAGGA AGCGCATGCG GGGCCCACGC CGATGCCGCG CCGCCGCGAC GCGCTGCTCG GCGCGTCCCG CGTCGTCGAT CTCGTGAACC GGATCGGCCT CGATCACGCG CCGTTCGGTT GCGCGACGGT CGGGATGATG CAGATTCATC CGAACTCGCG CAACGTGATT CCGGGCCGCG TGTTCTTCAC CGTCGATTTC CGTCATCCGG ACGATGCCGT GCTCGCGAAG ATGGACGCTG CGCTGCGCGA CGGCGTCGCG CGCATCGCGG GCGAGATCGG GCTCGAGACC GCGCTCGAGC AGATTTTCTA CTACAAGCCC GTCGCTTTCG ATCCGGCCTG CGTGGCGGCC GTGCGCGACG CGGCCGACCG CTTCGGCTAT TCGCATCGCG ACATCGTGTC GGGTGCGGGA CACGACGCGT GCTATCTGGC GCAGGTCGCG CCGACGTCGA TGGTGTTCGT GCCCTGCGTC GACGGCATCA GCCACAACGA GATCGAGGAC GCGACGCCTG CGTGGATCGA GGCAGGCGCG AACGTGCTGC TGCACGCGAT GCTCTCGCGT GCGTGCGAGC CGGCTTCA
Details for BuamA.12245.d.B1.GE39807
HARVESTED ON: 2/4/2016
SEQUENCED ON: 1/27/2016
EXPECTED MW: 47kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMD TVSETVKRAA FDTSIKVDGR RLWDSLMEVA KIGATPKGGV CRLALTDLDK AGRDLIVGWA KAAGCTVTVD TMGNVFMRRA GRVADAAPVV TGSHADSQPT GGRFDGIYGV LGGLEVIRSL NDHGIETEHP IEVVIWTNEE GSRFAPAMVA SGVFAGVFSL EYGLSRKDVD GKTIGEELAR IGYAGDVPCG GRKLHAAFEL HIEQGPILEA ECKTIGVVTD AQGQRWYEIT FTGQEAHAGP TPMPRRRDAL LGASRVVDLV NRIGLDHAPF GCATVGMMQI HPNSRNVIPG RVFFTVDFRH PDDAVLAKMD AALRDGVARI AGEIGLETAL EQIFYYKPVA FDPACVAAVR DAADRFGYSH RDIVSGAGHD ACYLAQVAPT SMVFVPCVDG ISHNEIEDAT PAWIEAGANV LLHAM
Validated NT Sequence
agcatcgcgt gcagcagcac gttcgcgcct gcctcgatcc acgcaggcgt cgcgtcctcg atctcgttgt ggctgatgcc gtcgacgcag ggcacgaaca ccatcgacgt cggcgcgacc tgcgccagat agcacgcgtc gtgtcccgca cccgacacga tgtcgcgatg cgaatagccg aagcggtcgg ccgcgtcgcg cacggccgcc acgcaggccg gatcgaaagc gacgggcttg tagtagaaaa tctgctcgag cgcggtctcg agcccgatct cgcccgcgat gcgcgcgacg ccgtcgcgca gcgcagcgtc catcttcgcg agcacggcat cgtccggatg acggaaatcg acggtgaaga acacgcggcc cggaatcacg ttgcgcgagt tcggatgaat ctgcatcatc ccgaccgtcg cgcaaccgaa cggcgcgtga tcgaggccga tccggttcac gagatcgacg acgcgggacg cgccgagcag cgcgtcgcgg cggcgcggca tcggcgtggg ccccgcatgc gcttcctggc cggtgaacgt gatctcatac cagcgctgcc cctgcgcgtc ggtcaccacg ccgatcgtct tgcactccgc ttcgagaatc ggcccctgtt cgatatgcag ctcgaacgcc gcatgcagct tgcggccgcc gcacggcacg tcgccggcat agccgatgcg cgccagttcc tcgccgatcg tcttgccgtc gacgtccttg cgcgacagcc cgtattcgag cgaaaacacg cccgcgaaca cgcccgacgc gaccatcgcc ggtgcgaagc gcgagccttc ctcgttggtc cagatcacga cctcgatcgg atgctcggtc tcgatgccgt gatcgttcag actgcgaatc acctcgaggc cgccgagcac gccgtagatg ccgtcgaagc ggccgcccgt cggctgcgaa tccgcgtgcg agccggtgac gaccggcgcc gcgtcggcca cgcgccccgc gcgacgcatg aatacgttgc ccatcgtatc gaccgtcacc gtgcagcccg ccgccttcgc ccagccgacg atcaggtcgc ggccggcctt gtcgagatcg gtcagcgcga ggcggcagac gccgcccttc ggcgtcgcgc cgatcttcgc cacctccatc aggctgtccc acaaccgtct gccgtcgacc ttgatcgacg tgtcgaatgc tgcccgcttc actgtttccg ataccgtgtc catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMD TVSETVKRAA FDTSIKVDGR RLWDSLMEVA KIGATPKGGV CRLALTDLDK AGRDLIVGWA KAAGCTVTVD TMGNVFMRRA GRVADAAPVV TGSHADSQPT GGRFDGIYGV LGGLEVIRSL NDHGIETEHP IEVVIWTNEE GSRFAPAMVA SGVFAGVFSL EYGLSRKDVD GKTIGEELAR IGYAGDVPCG GRKLHAAFEL HIEQGPILEA ECKTIGVVTD AQGQRWYEIT FTGQEAHAGP TPMPRRRDAL LGASRVVDLV NRIGLDHAPF GCATVGMMQI HPNSRNVIPG RVFFTVDFRH PDDAVLAKMD AALRDGVARI AGEIGLETAL EQIFYYKPVA FDPACVAAVR DAADRFGYSH RDIVSGAGHD ACYLAQVAPT SMVFVPCVDG ISHNEIEDAT PAWIEAGANV LLHAMLSRAC EPAS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaca cggtatcgga aacagtgaag cgggcagcat tcgacacgtc gatcaaggtc gacggcagac ggttgtggga cagcctgatg gaggtggcga agatcggcgc gacgccgaag ggcggcgtct gccgcctcgc gctgaccgat ctcgacaagg ccggccgcga cctgatcgtc ggctgggcga aggcggcggg ctgcacggtg acggtcgata cgatgggcaa cgtattcatg cgtcgcgcgg ggcgcgtggc cgacgcggcg ccggtcgtca ccggctcgca cgcggattcg cagccgacgg gcggccgctt cgacggcatc tacggcgtgc tcggcggcct cgaggtgatt cgcagtctga acgatcacgg catcgagacc gagcatccga tcgaggtcgt gatctggacc aacgaggaag gctcgcgctt cgcaccggcg atggtcgcgt cgggcgtgtt cgcgggcgtg ttttcgctcg aatacgggct gtcgcgcaag gacgtcgacg gcaagacgat cggcgaggaa ctggcgcgca tcggctatgc cggcgacgtg ccgtgcggcg gccgcaagct gcatgcggcg ttcgagctgc atatcgaaca ggggccgatt ctcgaagcgg agtgcaagac gatcggcgtg gtgaccgacg cgcaggggca gcgctggtat gagatcacgt tcaccggcca ggaagcgcat gcggggccca cgccgatgcc gcgccgccgc gacgcgctgc tcggcgcgtc ccgcgtcgtc gatctcgtga accggatcgg cctcgatcac gcgccgttcg gttgcgcgac ggtcgggatg atgcagattc atccgaactc gcgcaacgtg attccgggcc gcgtgttctt caccgtcgat ttccgtcatc cggacgatgc cgtgctcgcg aagatggacg ctgcgctgcg cgacggcgtc gcgcgcatcg cgggcgagat cgggctcgag accgcgctcg agcagatttt ctactacaag cccgtcgctt tcgatccggc ctgcgtggcg gccgtgcgcg acgcggccga ccgcttcggc tattcgcatc gcgacatcgt gtcgggtgcg ggacacgacg cgtgctatct ggcgcaggtc gcgccgacgt cgatggtgtt cgtgccctgc gtcgacggca tcagccacaa cgagatcgag gacgcgacgc ctgcgtggat cgaggcaggc gcgaacgtgc tgctgcacgc gatgctctcg cgtgcgtgcg agccggcttc atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for BuamA.12245.d.B1.PS37908
PURIFICATION DATe: 4/13/2016
CONCENTRATION: 32.6mg/ml
OBSERVED MW: 47kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC Buffer
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMD TVSETVKRAA FDTSIKVDGR RLWDSLMEVA KIGATPKGGV CRLALTDLDK AGRDLIVGWA KAAGCTVTVD TMGNVFMRRA GRVADAAPVV TGSHADSQPT GGRFDGIYGV LGGLEVIRSL NDHGIETEHP IEVVIWTNEE GSRFAPAMVA SGVFAGVFSL EYGLSRKDVD GKTIGEELAR IGYAGDVPCG GRKLHAAFEL HIEQGPILEA ECKTIGVVTD AQGQRWYEIT FTGQEAHAGP TPMPRRRDAL LGASRVVDLV NRIGLDHAPF GCATVGMMQI HPNSRNVIPG RVFFTVDFRH PDDAVLAKMD AALRDGVARI AGEIGLETAL EQIFYYKPVA FDPACVAAVR DAADRFGYSH RDIVSGAGHD ACYLAQVAPT SMVFVPCVDG ISHNEIEDAT PAWIEAGANV LLHAM
Validated NT Sequence
agcatcgcgt gcagcagcac gttcgcgcct gcctcgatcc acgcaggcgt cgcgtcctcg atctcgttgt ggctgatgcc gtcgacgcag ggcacgaaca ccatcgacgt cggcgcgacc tgcgccagat agcacgcgtc gtgtcccgca cccgacacga tgtcgcgatg cgaatagccg aagcggtcgg ccgcgtcgcg cacggccgcc acgcaggccg gatcgaaagc gacgggcttg tagtagaaaa tctgctcgag cgcggtctcg agcccgatct cgcccgcgat gcgcgcgacg ccgtcgcgca gcgcagcgtc catcttcgcg agcacggcat cgtccggatg acggaaatcg acggtgaaga acacgcggcc cggaatcacg ttgcgcgagt tcggatgaat ctgcatcatc ccgaccgtcg cgcaaccgaa cggcgcgtga tcgaggccga tccggttcac gagatcgacg acgcgggacg cgccgagcag cgcgtcgcgg cggcgcggca tcggcgtggg ccccgcatgc gcttcctggc cggtgaacgt gatctcatac cagcgctgcc cctgcgcgtc ggtcaccacg ccgatcgtct tgcactccgc ttcgagaatc ggcccctgtt cgatatgcag ctcgaacgcc gcatgcagct tgcggccgcc gcacggcacg tcgccggcat agccgatgcg cgccagttcc tcgccgatcg tcttgccgtc gacgtccttg cgcgacagcc cgtattcgag cgaaaacacg cccgcgaaca cgcccgacgc gaccatcgcc ggtgcgaagc gcgagccttc ctcgttggtc cagatcacga cctcgatcgg atgctcggtc tcgatgccgt gatcgttcag actgcgaatc acctcgaggc cgccgagcac gccgtagatg ccgtcgaagc ggccgcccgt cggctgcgaa tccgcgtgcg agccggtgac gaccggcgcc gcgtcggcca cgcgccccgc gcgacgcatg aatacgttgc ccatcgtatc gaccgtcacc gtgcagcccg ccgccttcgc ccagccgacg atcaggtcgc ggccggcctt gtcgagatcg gtcagcgcga ggcggcagac gccgcccttc ggcgtcgcgc cgatcttcgc cacctccatc aggctgtccc acaaccgtct gccgtcgacc ttgatcgacg tgtcgaatgc tgcccgcttc actgtttccg ataccgtgtc catatggtgg tggtggtggt gagccat
Expressed Protein Sequence
MAHHHHHHMD TVSETVKRAA FDTSIKVDGR RLWDSLMEVA KIGATPKGGV CRLALTDLDK AGRDLIVGWA KAAGCTVTVD TMGNVFMRRA GRVADAAPVV TGSHADSQPT GGRFDGIYGV LGGLEVIRSL NDHGIETEHP IEVVIWTNEE GSRFAPAMVA SGVFAGVFSL EYGLSRKDVD GKTIGEELAR IGYAGDVPCG GRKLHAAFEL HIEQGPILEA ECKTIGVVTD AQGQRWYEIT FTGQEAHAGP TPMPRRRDAL LGASRVVDLV NRIGLDHAPF GCATVGMMQI HPNSRNVIPG RVFFTVDFRH PDDAVLAKMD AALRDGVARI AGEIGLETAL EQIFYYKPVA FDPACVAAVR DAADRFGYSH RDIVSGAGHD ACYLAQVAPT SMVFVPCVDG ISHNEIEDAT PAWIEAGANV LLHAMLSRAC EPAS
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGACA CGGTATCGGA AACAGTGAAG CGGGCAGCAT TCGACACGTC GATCAAGGTC GACGGCAGAC GGTTGTGGGA CAGCCTGATG GAGGTGGCGA AGATCGGCGC GACGCCGAAG GGCGGCGTCT GCCGCCTCGC GCTGACCGAT CTCGACAAGG CCGGCCGCGA CCTGATCGTC GGCTGGGCGA AGGCGGCGGG CTGCACGGTG ACGGTCGATA CGATGGGCAA CGTATTCATG CGTCGCGCGG GGCGCGTGGC CGACGCGGCG CCGGTCGTCA CCGGCTCGCA CGCGGATTCG CAGCCGACGG GCGGCCGCTT CGACGGCATC TACGGCGTGC TCGGCGGCCT CGAGGTGATT CGCAGTCTGA ACGATCACGG CATCGAGACC GAGCATCCGA TCGAGGTCGT GATCTGGACC AACGAGGAAG GCTCGCGCTT CGCACCGGCG ATGGTCGCGT CGGGCGTGTT CGCGGGCGTG TTTTCGCTCG AATACGGGCT GTCGCGCAAG GACGTCGACG GCAAGACGAT CGGCGAGGAA CTGGCGCGCA TCGGCTATGC CGGCGACGTG CCGTGCGGCG GCCGCAAGCT GCATGCGGCG TTCGAGCTGC ATATCGAACA GGGGCCGATT CTCGAAGCGG AGTGCAAGAC GATCGGCGTG GTGACCGACG CGCAGGGGCA GCGCTGGTAT GAGATCACGT TCACCGGCCA GGAAGCGCAT GCGGGGCCCA CGCCGATGCC GCGCCGCCGC GACGCGCTGC TCGGCGCGTC CCGCGTCGTC GATCTCGTGA ACCGGATCGG CCTCGATCAC GCGCCGTTCG GTTGCGCGAC GGTCGGGATG ATGCAGATTC ATCCGAACTC GCGCAACGTG ATTCCGGGCC GCGTGTTCTT CACCGTCGAT TTCCGTCATC CGGACGATGC CGTGCTCGCG AAGATGGACG CTGCGCTGCG CGACGGCGTC GCGCGCATCG CGGGCGAGAT CGGGCTCGAG ACCGCGCTCG AGCAGATTTT CTACTACAAG CCCGTCGCTT TCGATCCGGC CTGCGTGGCG GCCGTGCGCG ACGCGGCCGA CCGCTTCGGC TATTCGCATC GCGACATCGT GTCGGGTGCG GGACACGACG CGTGCTATCT GGCGCAGGTC GCGCCGACGT CGATGGTGTT CGTGCCCTGC GTCGACGGCA TCAGCCACAA CGAGATCGAG GACGCGACGC CTGCGTGGAT CGAGGCAGGC GCGAACGTGC TGCTGCACGC GATGCTCTCG CGTGCGTGCG AGCCGGCTTC ATGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT