BuamA.12245.a
Amidase, hydantoinase/carbamoylase family
| CENTER ID: | BuamA.12245.a |
| ORGANISM: | Burkholderia ambifaria MC40-6 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuamA.12245.a.B1.GE39805 | Full length( BuamA.12245.a ) | 1 | 423 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuamA.12245.a.B1.PS37939 | Full length( BuamA.12245.a ) | 1 | 423 |
Structures
5TP4
DEPOSITED: 10/19/2016
DETERMINATION: XRay
CLONE: BuamA.12245.a.B1.GE39805
PROTEIN: BuamA.12245.a.B1.PS37939
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|398577.6.peg.1174 |
| RefSeq: | YP_001807851.1 |
| UniProt: | B1YW99 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKDLLEIDGA RLWRSLADMA RIGATPRGGV RRLALTDDDR RGRDLFAQWC RDAGMTVSVD AVGNLFARRD GADAQAAPVL IGSHLDTQPE GGRFDGVYGV LAGLEVVRTL NDAGIVTDKP LEIVSWTNEE GARFAPAMLG SAVFTGALPL DDALARQDAE GITLGAALDA CGCRGTRAPG GAVDAYFEAH IEQGPVLEAN GTTIGIVTGG QAIRWLDVRV TGVAAHAGTT PMPYRKDAYF ASAQMALELE RIVAGHAPRG LATIGQAGIR NASRNTIAGD VTFTVDLRHH DDAQVDAMER ALRDACARVA AARGVQVAID TCWRSPATPF DRGCVELVAR AAEAFGYTNE RIVSGAGHDA ILLARRVPTA MVFIPCVDGL SHNEAEDALP DDVTRGTNVL LNAVLARAGV ATRVAAAAAA HDA
NT Sequence
ATGAAAGACC TGCTGGAAAT CGACGGTGCC CGCCTGTGGC GGAGCCTGGC CGACATGGCG CGCATCGGCG CGACGCCGCG CGGCGGCGTG CGGCGCCTCG CGCTGACCGA CGACGACCGG CGCGGCCGCG ACCTGTTCGC GCAGTGGTGC CGCGACGCGG GGATGACGGT GAGCGTCGAC GCGGTCGGCA ACCTGTTCGC GCGGCGCGAC GGCGCCGATG CGCAGGCCGC GCCGGTGCTG ATCGGCAGCC ATCTCGACAC GCAGCCCGAA GGCGGGCGCT TCGACGGCGT GTACGGCGTG CTCGCCGGGC TCGAAGTCGT GCGCACGCTG AACGATGCGG GCATCGTGAC CGACAAGCCG CTCGAGATCG TGTCGTGGAC CAACGAGGAG GGCGCGCGTT TCGCGCCGGC GATGCTCGGC TCGGCCGTGT TCACGGGCGC GCTGCCGCTC GACGACGCGC TCGCGAGGCA GGACGCCGAG GGCATCACGC TCGGCGCCGC GCTGGACGCA TGCGGCTGTC GCGGCACGCG TGCGCCGGGC GGCGCGGTCG ACGCGTATTT CGAAGCGCAT ATCGAACAGG GGCCCGTGCT CGAGGCGAAC GGCACGACGA TCGGCATCGT CACGGGCGGG CAGGCGATCC GCTGGCTCGA CGTGCGCGTG ACGGGGGTGG CCGCGCATGC GGGCACGACG CCGATGCCGT ATCGCAAGGA CGCGTATTTC GCGAGCGCGC AGATGGCGCT CGAACTGGAA CGCATCGTCG CCGGCCACGC GCCGCGCGGG CTCGCGACGA TCGGGCAGGC CGGCATCCGC AACGCGTCGC GCAACACGAT CGCCGGCGAC GTGACGTTCA CCGTCGACCT GCGCCATCAC GACGATGCGC AAGTCGATGC GATGGAGCGC GCGCTGCGCG ACGCATGCGC GCGCGTGGCC GCTGCGCGCG GCGTGCAGGT CGCGATCGAT ACCTGCTGGC GCAGCCCCGC GACGCCGTTC GACCGCGGCT GCGTCGAGCT GGTCGCGCGG GCCGCGGAGG CATTTGGTTA CACGAACGAA CGGATCGTCA GCGGCGCCGG TCACGATGCG ATCCTGCTCG CGCGTCGCGT ACCGACCGCG ATGGTGTTCA TCCCGTGCGT CGACGGGCTG TCGCACAACG AGGCCGAGGA CGCGCTGCCC GACGACGTGA CGCGCGGCAC CAACGTGCTG CTCAACGCGG TGCTCGCGCG GGCGGGCGTC GCGACGCGCG TGGCGGCAGC GGCAGCCGCG CACGATGCA
Details for BuamA.12245.a.B1.GE39805
| HARVESTED ON: | 2/4/2016 |
| SEQUENCED ON: | 1/27/2016 |
| EXPECTED MW: | 45kDa |
| OBSERVED MW: | 45kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 73 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHMK DLLEIDGARL WRSLADMARI GATPRGGVRR LALTDDDRRG RDLFAQWCRD AGMTVSVDAV GNLFARRDGA DAQAAPVLIG SHLDTQPEGG RFDGVYGVLA GLEVVRTLND AGIVTDKPLE IVSWTNEEGA RFAPAMLGSA VFTGALPLDD ALARQDAEGI TLGAALDACG CRGTRAPGGA VDAYFEAHIE QGPVLEANGT TIGIVTGGQA IRWLDVRVTG VAAHAGTTPM PYRKDAYFAS AQMALELERI VAGHAPRGLA TXXAGRHPQR VAQHDRRRRD VHRRPAPSRR CASRCDGARA ARRMRARGRC ARRAGRDRYL LAQPRDAVRP RLRRAGRAGR GGIWLHERTD RQRRRSRCDP ARASRTDRDG VHPVRRRAVA QRGRGRAARR RDARHQRAAQ RGARAGGRRD AR
Validated NT Sequence
cacgcgcgtc gcgacgcccg cccgcgcgag caccgcgttg agcagcacgt tggtgccgcg cgtcacgtcg tcgggcagcg cgtcctcggc ctcgttgtgc gacagcccgt cgacgcacgg gatgaacacc atcgcggtcg gtacgcgacg cgcgagcagg atcgcatcgt gaccggcgcc gctgacgatc cgttcgttcg tgtaaccaaa tgcctccgcg gcccgcgcga ccagctcgac gcagccgcgg tcgaacggcg tcgcggggct gcgccagcag gtatcgatcg cgacctgcac gccgcgcgca gcggccacgc gcgcgcatgc gtcgcgcagc gcgcgctcca tcgcatcgac ttgcgcatcg tcgtgatggc gcaggtcgac ggtgaacgtc acgtcgccgg cgatcgtgtt gcgcgacgcg ttgcggatgc cggcctgccc nnatcgtcgc gagcccgcgc ggcgcgtggc cggcgacgat gcgttccagt tcgagcgcca tctgcgcgct cgcgaaatac gcgtccttgc gatacggcat cggcgtcgtg cccgcatgcg cggccacccc cgtcacgcgc acgtcgagcc agcggatcgc ctgcccgccc gtgacgatgc cgatcgtcgt gccgttcgcc tcgagcacgg gcccctgttc gatatgcgct tcgaaatacg cgtcgaccgc gccgcccggc gcacgcgtgc cgcgacagcc gcatgcgtcc agcgcggcgc cgagcgtgat gccctcggcg tcctgcctcg cgagcgcgtc gtcgagcggc agcgcgcccg tgaacacggc cgagccgagc atcgccggcg cgaaacgcgc gccctcctcg ttggtccacg acacgatctc gagcggcttg tcggtcacga tgcccgcatc gttcagcgtg cgcacgactt cgagcccggc gagcacgccg tacacgccgt cgaagcgccc gccttcgggc tgcgtgtcga gatggctgcc gatcagcacc ggcgcggcct gcgcatcggc gccgtcgcgc cgcgcgaaca ggttgccgac cgcgtcgacg ctcaccgtca tccccgcgtc gcggcaccac tgcgcgaaca ggtcgcggcc gcgccggtcg tcgtcggtca gcgcgaggcg ccgcacgccg ccgcgcggcg tcgcgccgat gcgcgccatg tcggccaggc tccgccacag gcgggcaccg tcgatttcca gcaggtcttt catatggtgg tggtggtggt gagccatggt atntctc
Expected Protein Sequence
MAHHHHHHMK DLLEIDGARL WRSLADMARI GATPRGGVRR LALTDDDRRG RDLFAQWCRD AGMTVSVDAV GNLFARRDGA DAQAAPVLIG SHLDTQPEGG RFDGVYGVLA GLEVVRTLND AGIVTDKPLE IVSWTNEEGA RFAPAMLGSA VFTGALPLDD ALARQDAEGI TLGAALDACG CRGTRAPGGA VDAYFEAHIE QGPVLEANGT TIGIVTGGQA IRWLDVRVTG VAAHAGTTPM PYRKDAYFAS AQMALELERI VAGHAPRGLA TIGQAGIRNA SRNTIAGDVT FTVDLRHHDD AQVDAMERAL RDACARVAAA RGVQVAIDTC WRSPATPFDR GCVELVARAA EAFGYTNERI VSGAGHDAIL LARRVPTAMV FIPCVDGLSH NEAEDALPDD VTRGTNVLLN AVLARAGVAT RVAAAAAAHD A
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaag acctgctgga aatcgacggt gcccgcctgt ggcggagcct ggccgacatg gcgcgcatcg gcgcgacgcc gcgcggcggc gtgcggcgcc tcgcgctgac cgacgacgac cggcgcggcc gcgacctgtt cgcgcagtgg tgccgcgacg cggggatgac ggtgagcgtc gacgcggtcg gcaacctgtt cgcgcggcgc gacggcgccg atgcgcaggc cgcgccggtg ctgatcggca gccatctcga cacgcagccc gaaggcgggc gcttcgacgg cgtgtacggc gtgctcgccg ggctcgaagt cgtgcgcacg ctgaacgatg cgggcatcgt gaccgacaag ccgctcgaga tcgtgtcgtg gaccaacgag gagggcgcgc gtttcgcgcc ggcgatgctc ggctcggccg tgttcacggg cgcgctgccg ctcgacgacg cgctcgcgag gcaggacgcc gagggcatca cgctcggcgc cgcgctggac gcatgcggct gtcgcggcac gcgtgcgccg ggcggcgcgg tcgacgcgta tttcgaagcg catatcgaac aggggcccgt gctcgaggcg aacggcacga cgatcggcat cgtcacgggc gggcaggcga tccgctggct cgacgtgcgc gtgacggggg tggccgcgca tgcgggcacg acgccgatgc cgtatcgcaa ggacgcgtat ttcgcgagcg cgcagatggc gctcgaactg gaacgcatcg tcgccggcca cgcgccgcgc gggctcgcga cgatcgggca ggccggcatc cgcaacgcgt cgcgcaacac gatcgccggc gacgtgacgt tcaccgtcga cctgcgccat cacgacgatg cgcaagtcga tgcgatggag cgcgcgctgc gcgacgcatg cgcgcgcgtg gccgctgcgc gcggcgtgca ggtcgcgatc gatacctgct ggcgcagccc cgcgacgccg ttcgaccgcg gctgcgtcga gctggtcgcg cgggccgcgg aggcatttgg ttacacgaac gaacggatcg tcagcggcgc cggtcacgat gcgatcctgc tcgcgcgtcg cgtaccgacc gcgatggtgt tcatcccgtg cgtcgacggg ctgtcgcaca acgaggccga ggacgcgctg cccgacgacg tgacgcgcgg caccaacgtg ctgctcaacg cggtgctcgc gcgggcgggc gtcgcgacgc gcgtggcggc agcggcagcc gcgcacgatg catgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for BuamA.12245.a.B1.PS37939
| PURIFICATION DATe: | 6/9/2016 |
| CONCENTRATION: | 123mg/ml |
| OBSERVED MW: | 46kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | SEC |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 15 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 73 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMK DLLEIDGARL WRSLADMARI GATPRGGVRR LALTDDDRRG RDLFAQWCRD AGMTVSVDAV GNLFARRDGA DAQAAPVLIG SHLDTQPEGG RFDGVYGVLA GLEVVRTLND AGIVTDKPLE IVSWTNEEGA RFAPAMLGSA VFTGALPLDD ALARQDAEGI TLGAALDACG CRGTRAPGGA VDAYFEAHIE QGPVLEANGT TIGIVTGGQA IRWLDVRVTG VAAHAGTTPM PYRKDAYFAS AQMALELERI VAGHAPRGLA TXXAGRHPQR VAQHDRRRRD VHRRPAPSRR CASRCDGARA ARRMRARGRC ARRAGRDRYL LAQPRDAVRP RLRRAGRAGR GGIWLHERTD RQRRRSRCDP ARASRTDRDG VHPVRRRAVA QRGRGRAARR RDARHQRAAQ RGARAGGRRD AR
Validated NT Sequence
cacgcgcgtc gcgacgcccg cccgcgcgag caccgcgttg agcagcacgt tggtgccgcg cgtcacgtcg tcgggcagcg cgtcctcggc ctcgttgtgc gacagcccgt cgacgcacgg gatgaacacc atcgcggtcg gtacgcgacg cgcgagcagg atcgcatcgt gaccggcgcc gctgacgatc cgttcgttcg tgtaaccaaa tgcctccgcg gcccgcgcga ccagctcgac gcagccgcgg tcgaacggcg tcgcggggct gcgccagcag gtatcgatcg cgacctgcac gccgcgcgca gcggccacgc gcgcgcatgc gtcgcgcagc gcgcgctcca tcgcatcgac ttgcgcatcg tcgtgatggc gcaggtcgac ggtgaacgtc acgtcgccgg cgatcgtgtt gcgcgacgcg ttgcggatgc cggcctgccc nnatcgtcgc gagcccgcgc ggcgcgtggc cggcgacgat gcgttccagt tcgagcgcca tctgcgcgct cgcgaaatac gcgtccttgc gatacggcat cggcgtcgtg cccgcatgcg cggccacccc cgtcacgcgc acgtcgagcc agcggatcgc ctgcccgccc gtgacgatgc cgatcgtcgt gccgttcgcc tcgagcacgg gcccctgttc gatatgcgct tcgaaatacg cgtcgaccgc gccgcccggc gcacgcgtgc cgcgacagcc gcatgcgtcc agcgcggcgc cgagcgtgat gccctcggcg tcctgcctcg cgagcgcgtc gtcgagcggc agcgcgcccg tgaacacggc cgagccgagc atcgccggcg cgaaacgcgc gccctcctcg ttggtccacg acacgatctc gagcggcttg tcggtcacga tgcccgcatc gttcagcgtg cgcacgactt cgagcccggc gagcacgccg tacacgccgt cgaagcgccc gccttcgggc tgcgtgtcga gatggctgcc gatcagcacc ggcgcggcct gcgcatcggc gccgtcgcgc cgcgcgaaca ggttgccgac cgcgtcgacg ctcaccgtca tccccgcgtc gcggcaccac tgcgcgaaca ggtcgcggcc gcgccggtcg tcgtcggtca gcgcgaggcg ccgcacgccg ccgcgcggcg tcgcgccgat gcgcgccatg tcggccaggc tccgccacag gcgggcaccg tcgatttcca gcaggtcttt catatggtgg tggtggtggt gagccatggt atntctc
Expressed Protein Sequence
MAHHHHHHMK DLLEIDGARL WRSLADMARI GATPRGGVRR LALTDDDRRG RDLFAQWCRD AGMTVSVDAV GNLFARRDGA DAQAAPVLIG SHLDTQPEGG RFDGVYGVLA GLEVVRTLND AGIVTDKPLE IVSWTNEEGA RFAPAMLGSA VFTGALPLDD ALARQDAEGI TLGAALDACG CRGTRAPGGA VDAYFEAHIE QGPVLEANGT TIGIVTGGQA IRWLDVRVTG VAAHAGTTPM PYRKDAYFAS AQMALELERI VAGHAPRGLA TIGQAGIRNA SRNTIAGDVT FTVDLRHHDD AQVDAMERAL RDACARVAAA RGVQVAIDTC WRSPATPFDR GCVELVARAA EAFGYTNERI VSGAGHDAIL LARRVPTAMV FIPCVDGLSH NEAEDALPDD VTRGTNVLLN AVLARAGVAT RVAAAAAAHD A
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAAG ACCTGCTGGA AATCGACGGT GCCCGCCTGT GGCGGAGCCT GGCCGACATG GCGCGCATCG GCGCGACGCC GCGCGGCGGC GTGCGGCGCC TCGCGCTGAC CGACGACGAC CGGCGCGGCC GCGACCTGTT CGCGCAGTGG TGCCGCGACG CGGGGATGAC GGTGAGCGTC GACGCGGTCG GCAACCTGTT CGCGCGGCGC GACGGCGCCG ATGCGCAGGC CGCGCCGGTG CTGATCGGCA GCCATCTCGA CACGCAGCCC GAAGGCGGGC GCTTCGACGG CGTGTACGGC GTGCTCGCCG GGCTCGAAGT CGTGCGCACG CTGAACGATG CGGGCATCGT GACCGACAAG CCGCTCGAGA TCGTGTCGTG GACCAACGAG GAGGGCGCGC GTTTCGCGCC GGCGATGCTC GGCTCGGCCG TGTTCACGGG CGCGCTGCCG CTCGACGACG CGCTCGCGAG GCAGGACGCC GAGGGCATCA CGCTCGGCGC CGCGCTGGAC GCATGCGGCT GTCGCGGCAC GCGTGCGCCG GGCGGCGCGG TCGACGCGTA TTTCGAAGCG CATATCGAAC AGGGGCCCGT GCTCGAGGCG AACGGCACGA CGATCGGCAT CGTCACGGGC GGGCAGGCGA TCCGCTGGCT CGACGTGCGC GTGACGGGGG TGGCCGCGCA TGCGGGCACG ACGCCGATGC CGTATCGCAA GGACGCGTAT TTCGCGAGCG CGCAGATGGC GCTCGAACTG GAACGCATCG TCGCCGGCCA CGCGCCGCGC GGGCTCGCGA CGATCGGGCA GGCCGGCATC CGCAACGCGT CGCGCAACAC GATCGCCGGC GACGTGACGT TCACCGTCGA CCTGCGCCAT CACGACGATG CGCAAGTCGA TGCGATGGAG CGCGCGCTGC GCGACGCATG CGCGCGCGTG GCCGCTGCGC GCGGCGTGCA GGTCGCGATC GATACCTGCT GGCGCAGCCC CGCGACGCCG TTCGACCGCG GCTGCGTCGA GCTGGTCGCG CGGGCCGCGG AGGCATTTGG TTACACGAAC GAACGGATCG TCAGCGGCGC CGGTCACGAT GCGATCCTGC TCGCGCGTCG CGTACCGACC GCGATGGTGT TCATCCCGTG CGTCGACGGG CTGTCGCACA ACGAGGCCGA GGACGCGCTG CCCGACGACG TGACGCGCGG CACCAACGTG CTGCTCAACG CGGTGCTCGC GCGGGCGGGC GTCGCGACGC GCGTGGCGGC AGCGGCAGCC GCGCACGATG CATGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT