BuamA.11726.a

N-acetylmuramoyl-L-alanine amidase

CENTER ID: BuamA.11726.a
ORGANISM: Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.11726.a.B2.GE43971 Mature protein( BuamA.11726.a ) 48 515
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|398577.6.peg.2553
RefSeq: YP_001809176.1
UniProt: B1YVF6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSRKMLIKPF RSIESAATAT HNWRRRQILR AGASTLVLGL AAPRLAHASS VLGVRVWPAR DYTRVTIESD QPLQNSQQLL QGPDRLVVDL SGLDLDQALR DLVSKIAPND PQIQSVRVGQ YQPHVVRMVF DLKGSVKPQV FTLPPVGTYK YRLVFDLYPA VAPDPLSDLI AQTERKEQAL NDTARAQQMQ PPTSLAGPAA PSAPAAGDST DAFFQRFAQN TPATPATPAT PRTPPAAATP STPAKPAVKP PPVIARRDDS DDDGDTYKFT APKSGKGGTM RLLTVAIDPG HGGEDPGAIG GGGTYEKHIA LDIAKKLRAK IDGAPNMRAM MTRDADFFVP LNVRVQKARR VGADLFVSIH ADAFTTPSAR GSSVFALSDH GATSAAARWM ANKENSSDLI GGINIKTQDA AVSRALFDMS TTAQIRDSLR YGGYVLKEVG GINKLHKGSV EQAGFAVLKA PDIPSILVET AFISNPEEES RLNDDSYRDQ MADAIFRGIK RYFAANPPLA KNRMA
NT Sequence
ATGTCTCGAA AGATGTTGAT CAAACCGTTC CGCTCGATCG AATCGGCGGC CACCGCGACG CACAACTGGC GGCGCCGCCA GATCCTGCGC GCGGGCGCGT CGACGCTGGT GCTCGGGCTC GCCGCACCGC GGCTCGCGCA CGCGTCGTCG GTGCTCGGCG TGCGCGTGTG GCCCGCGCGC GATTACACGC GCGTCACGAT CGAATCGGAC CAGCCGCTGC AGAATAGCCA ACAGCTGTTG CAGGGCCCCG ACCGGCTCGT CGTCGACCTG AGCGGGCTCG ATCTCGATCA GGCCCTGCGC GACCTCGTGT CGAAGATCGC GCCGAACGAT CCGCAGATCC AGTCGGTGCG CGTCGGTCAG TACCAGCCGC ACGTCGTGCG GATGGTGTTC GACCTGAAAG GCTCGGTGAA GCCGCAGGTG TTCACGCTGC CGCCGGTGGG CACCTACAAG TACCGGCTCG TGTTCGACCT GTATCCGGCC GTCGCGCCCG ATCCGCTGTC CGACCTGATC GCGCAGACGG AACGCAAGGA ACAGGCGCTC AACGACACCG CGCGCGCGCA GCAGATGCAG CCGCCGACCT CGCTCGCGGG CCCGGCCGCG CCGTCGGCGC CCGCCGCGGG CGACAGCACC GACGCGTTCT TCCAGCGCTT CGCGCAGAAC ACGCCGGCCA CGCCGGCCAC GCCGGCCACG CCGCGCACGC CGCCGGCCGC CGCGACGCCC TCCACGCCCG CGAAACCGGC CGTCAAGCCG CCGCCCGTCA TCGCGCGCCG CGACGACAGC GACGACGACG GCGACACCTA CAAGTTCACC GCACCCAAAT CCGGCAAGGG CGGCACCATG CGCCTGCTGA CGGTCGCGAT CGATCCGGGC CACGGCGGCG AGGATCCGGG CGCGATCGGC GGCGGCGGCA CGTACGAGAA GCACATCGCG CTCGACATCG CGAAGAAGCT GCGCGCGAAG ATCGACGGCG CGCCGAACAT GCGCGCGATG ATGACGCGCG ACGCCGACTT CTTCGTGCCG CTGAACGTGC GCGTGCAGAA GGCGCGCCGC GTCGGCGCCG ACCTGTTCGT GTCGATCCAC GCGGATGCGT TCACGACGCC CTCCGCGCGC GGCTCGTCGG TGTTCGCGCT GTCCGACCAC GGCGCGACGA GCGCCGCGGC ACGCTGGATG GCGAACAAGG AAAACTCGTC GGACCTGATC GGCGGCATCA ACATCAAGAC GCAGGACGCG GCCGTGAGCC GCGCGCTGTT CGACATGTCG ACGACCGCGC AGATCCGCGA TTCGCTGCGC TACGGCGGCT ACGTGCTGAA GGAAGTCGGC GGCATCAACA AGCTGCACAA GGGCTCGGTC GAGCAGGCCG GATTCGCGGT GCTGAAGGCG CCCGACATTC CGTCGATCCT CGTCGAGACC GCGTTCATCA GCAACCCGGA AGAAGAGAGC CGGCTGAACG ACGACAGCTA TCGCGACCAG ATGGCCGACG CGATCTTCCG CGGCATCAAG CGTTACTTCG CCGCGAACCC GCCGCTCGCG AAGAACCGGA TGGCC
Details for BuamA.11726.a.B2.GE43971
HARVESTED ON: data unavailable
SEQUENCED ON: 12/16/2020
EXPECTED MW: 51kDa
OBSERVED MW: 51kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMA SSVLGVRVWP ARDYTRVTIE SDQPLQNSQQ LLQGPDRLVV DLSGLDLDQA LRDLVSKIAP NDPQIQSVRV GQYQPHVVRM VFDLKGSVKP QVFTLPPVGT YKYRLVFDLY PAVAPDPLSD LIAQTERKEQ ALNDTARAQQ MQPPTSLAGP AAPSAPAAGD STDAFFQRFA QNTPATPATP ATPRTPPAAA TPSTPAKPAV KPPPVIARRD DSDDDGDTYK FTAPKSGKGG TMRLLTVAID PGHGGEDPGA IGGGGTYEKH IALDIAKKLR AKIDGAPNMR AMMTRDADFF VPLNVRVQKA RRVGADLFVS IHADAFTTPS ARGSSVFALS DHGATSAAAR WMANKENSSD LIGGINIKTQ DAAVSRALFD MSTTAQIRDS LRYGGYVLKE VGGINKLHKG SVEQAGFAVL KAPDIPSILV ETAFISNPEE ESRLNDDSYR DQMADAIFRG IKRYFAANPP LAKNRMA
Validated NT Sequence
atggctcacc accaccacca ccatatggcg tcgtcggtgc tcggcgtgcg cgtgtggccc gcgcgcgatt acacgcgcgt cacgatcgaa tcggaccagc cgctgcagaa tagccaacag ctgttgcagg gccccgaccg gctcgtcgtc gacctgagcg ggctcgatct cgatcaggcc ctgcgcgacc tcgtgtcgaa gatcgcgccg aacgatccgc agatccagtc ggtgcgcgtc ggtcagtacc agccgcacgt cgtgcggatg gtgttcgacc tgaaaggctc ggtgaagccg caggtgttca cgctgccgcc ggtgggcacc tacaagtacc ggctcgtgtt cgacctgtat ccggccgtcg cgcccgatcc gctgtccgac ctgatcgcgc agacggaacg caaggaacag gcgctcaacg acaccgcgcg cgcgcagcag atgcagccgc cgacctcgct cgcgggcccg gccgcgccgt cggcgcccgc cgcgggcgac agcaccgacg cgttcttcca gcgcttcgcg cagaacacgc cggccacgcc ggnccacgcc ggccacgccg cgcacgccgc cggccgccgc gacgccctcc acgcccgcga aaccggccgt caagccgccg cccgtcatcg cgcgccgcga cgacagcgac gacgacggcg acacctacaa gttcaccgca cccaaatccg gcaagggcgg caccatgcgc ctgctgacgg tcgcgatcga tccgggccac ggcggcgagg atccgggcgc gatcggcggc ggcggcacgt acgagaagca catcgcgctc gacatcgcga agaagctgcg cgcgaagatc gacggcgcgc cgaacatgcg cgcgatgatg acgcgcgacg ccgacttctt cgtgccgctg aacgtgcgcg tgcagaaggc gcgccgcgtc ggcgccgacc tgttcgtgtc gatccacgcg gatgcgttca cgacgccctc cgcgcgcggc tcgtcggtgt tcgcgctgtc cgaccacggc gcgacgagcg ccgcggcacg ctggatggcg aacaaggaaa actcgtcgga cctgatcggc ggcatcaaca tcaagacgca ggacgcggcc gtgagccgcg cgctgttcga catgtcgacg accgcgcaga tccgcgattc gctgcgctac ggcggctacg tgctgaagga agtcggcggc atcaacaagc tgcacaaggg ctcggtcgag caggccggat tcgcggtgct gaaggcgccc gacattccgt cgatcctcgt cgagaccgcg ttcatcagca acccggaaga agagagccgg ctgaacgacg acagctatcg cgaccagatg gccgacgcga tcttccgcgg catcaagcgt tacttcgccg cgaacccgcc gc
Expected Protein Sequence
MAHHHHHHAS SVLGVRVWPA RDYTRVTIES DQPLQNSQQL LQGPDRLVVD LSGLDLDQAL RDLVSKIAPN DPQIQSVRVG QYQPHVVRMV FDLKGSVKPQ VFTLPPVGTY KYRLVFDLYP AVAPDPLSDL IAQTERKEQA LNDTARAQQM QPPTSLAGPA APSAPAAGDS TDAFFQRFAQ NTPATPATPA TPRTPPAAAT PSTPAKPAVK PPPVIARRDD SDDDGDTYKF TAPKSGKGGT MRLLTVAIDP GHGGEDPGAI GGGGTYEKHI ALDIAKKLRA KIDGAPNMRA MMTRDADFFV PLNVRVQKAR RVGADLFVSI HADAFTTPSA RGSSVFALSD HGATSAAARW MANKENSSDL IGGINIKTQD AAVSRALFDM STTAQIRDSL RYGGYVLKEV GGINKLHKGS VEQAGFAVLK APDIPSILVE TAFISNPEEE SRLNDDSYRD QMADAIFRGI KRYFAANPPL AKNRMA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcgtcgt cggtgctcgg cgtgcgcgtg tggcccgcgc gcgattacac gcgcgtcacg atcgaatcgg accagccgct gcagaatagc caacagctgt tgcagggccc cgaccggctc gtcgtcgacc tgagcgggct cgatctcgat caggccctgc gcgacctcgt gtcgaagatc gcgccgaacg atccgcagat ccagtcggtg cgcgtcggtc agtaccagcc gcacgtcgtg cggatggtgt tcgacctgaa aggctcggtg aagccgcagg tgttcacgct gccgccggtg ggcacctaca agtaccggct cgtgttcgac ctgtatccgg ccgtcgcgcc cgatccgctg tccgacctga tcgcgcagac ggaacgcaag gaacaggcgc tcaacgacac cgcgcgcgcg cagcagatgc agccgccgac ctcgctcgcg ggcccggccg cgccgtcggc gcccgccgcg ggcgacagca ccgacgcgtt cttccagcgc ttcgcgcaga acacgccggc cacgccggcc acgccggcca cgccgcgcac gccgccggcc gccgcgacgc cctccacgcc cgcgaaaccg gccgtcaagc cgccgcccgt catcgcgcgc cgcgacgaca gcgacgacga cggcgacacc tacaagttca ccgcacccaa atccggcaag ggcggcacca tgcgcctgct gacggtcgcg atcgatccgg gccacggcgg cgaggatccg ggcgcgatcg gcggcggcgg cacgtacgag aagcacatcg cgctcgacat cgcgaagaag ctgcgcgcga agatcgacgg cgcgccgaac atgcgcgcga tgatgacgcg cgacgccgac ttcttcgtgc cgctgaacgt gcgcgtgcag aaggcgcgcc gcgtcggcgc cgacctgttc gtgtcgatcc acgcggatgc gttcacgacg ccctccgcgc gcggctcgtc ggtgttcgcg ctgtccgacc acggcgcgac gagcgccgcg gcacgctgga tggcgaacaa ggaaaactcg tcggacctga tcggcggcat caacatcaag acgcaggacg cggccgtgag ccgcgcgctg ttcgacatgt cgacgaccgc gcagatccgc gattcgctgc gctacggcgg ctacgtgctg aaggaagtcg gcggcatcaa caagctgcac aagggctcgg tcgagcaggc cggattcgcg gtgctgaagg cgcccgacat tccgtcgatc ctcgtcgaga ccgcgttcat cagcaacccg gaagaagaga gccggctgaa cgacgacagc tatcgcgacc agatggccga cgcgatcttc cgcggcatca agcgttactt cgccgcgaac ccgccgctcg cgaagaaccg gatggcctga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at