BuamA.10611.c
Alcohol dehydrogenase zinc-binding domain protein
| CENTER ID: | BuamA.10611.c |
| ORGANISM: | Burkholderia ambifaria MC40-6 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuamA.10611.c.B1.GE39802 | Full length( BuamA.10611.c ) | 1 | 375 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuamA.10611.c.B1.PS37979 | Full length( BuamA.10611.c ) | 1 | 375 |
Structures
5TNX
DEPOSITED: 10/15/2016
DETERMINATION: XRay
CLONE: BuamA.10611.c.B1.GE39802
PROTEIN: BuamA.10611.c.B1.PS37979
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|398577.6.peg.6784 |
| RefSeq: | YP_001816292.1 |
| UniProt: | B1Z4S6 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MYTENDTRAV TAAVARAAGA PFSIEPARIR APRGDEVLVR VVATGLCHTD LIVRDQYYPV PLPAVLGHEG AGVVEAVGPN VKTLAAGDHV VLTYGACGHC ASCAGGHGAY CRQFFALNFG GADADGQTAL RDAAGEPLHD HFFAQSSFAS YALARENNAI KVPKEAPLEL LGPLGCGIQT GAGAVINSLA VRTGSSFASF GAGAVGMSAV MAARIAGATT IIAVDIVPSR LALALELGAT HAINSKEVDV VDAIREITGG GVDYALESTG LPAVLSQGID ALGSRGTMGV VGAPKLGTKA EFDVNSLLLG GHTIRGIVEG DSVPQTFIPQ LVQLHLQGRF PFDRLVKFYP LEQINQAAAD SSSGITLKPI LRLPH
NT Sequence
ATGTATACCG AAAACGATAC GCGCGCCGTC ACCGCGGCCG TCGCCCGCGC CGCCGGCGCG CCCTTCTCGA TCGAACCGGC CCGGATCCGC GCGCCGCGCG GCGACGAGGT GCTGGTGCGC GTCGTCGCGA CGGGCCTGTG CCACACCGAC CTGATCGTGC GCGACCAGTA CTATCCTGTG CCGCTACCGG CCGTGCTCGG TCATGAAGGC GCCGGCGTGG TCGAGGCCGT CGGCCCGAAC GTGAAGACGC TCGCCGCCGG CGATCACGTC GTGCTCACCT ACGGCGCGTG CGGCCACTGC GCGTCGTGCG CCGGCGGTCA CGGTGCGTAT TGCCGGCAGT TCTTCGCGCT GAACTTCGGC GGTGCCGATG CCGACGGACA GACGGCCCTC CGCGACGCAG CCGGCGAACC GCTGCACGAT CATTTCTTCG CGCAGTCGTC GTTCGCGAGC TACGCGCTCG CGCGCGAGAA CAACGCGATC AAGGTGCCGA AGGAAGCGCC GCTCGAACTG CTCGGCCCGC TCGGCTGCGG CATCCAGACC GGCGCGGGCG CGGTAATCAA TTCGCTCGCG GTACGCACGG GCAGCAGCTT CGCGAGCTTC GGCGCCGGCG CGGTCGGGAT GAGCGCCGTG ATGGCCGCGC GTATCGCCGG CGCGACGACC ATCATCGCCG TCGACATCGT GCCGTCGCGG CTCGCGCTGG CGCTGGAGCT CGGCGCGACG CACGCGATCA ACAGCAAGGA AGTCGACGTC GTCGACGCGA TCCGCGAGAT CACCGGCGGC GGCGTCGACT ACGCGCTCGA ATCGACCGGG CTGCCGGCCG TGCTGTCGCA AGGGATCGAC GCGCTCGGCT CGCGCGGCAC GATGGGCGTG GTCGGCGCGC CGAAGCTCGG CACGAAGGCC GAATTCGACG TGAACAGCCT GCTGCTCGGC GGCCACACGA TCCGCGGGAT CGTCGAAGGC GACAGCGTGC CGCAGACCTT CATTCCGCAA CTCGTGCAGC TGCATCTGCA AGGCCGGTTC CCCTTCGACC GGCTGGTGAA GTTCTATCCG CTCGAGCAGA TCAACCAGGC CGCCGCGGAC AGCAGCAGCG GCATCACGCT CAAACCGATC CTGCGGTTGC CGCAT
Details for BuamA.10611.c.B1.GE39802
| HARVESTED ON: | 2/4/2016 |
| SEQUENCED ON: | 1/27/2016 |
| EXPECTED MW: | 40kDa |
| OBSERVED MW: | 40kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 98 |
Validated AA Sequence
MAHHHHHHHM YTENDTRAVT AAVARAAGAP FSIEPARIRA PRGDEVLVRV VATGLCHTDL IVRDQYYPVP LPAVLGHEGA GVVEAVGPNV KTLAAGDHVV LTYGACGHCA SCAGGHGAYC RQFFALNFGG ADADGQTALR DAAGEPLHDH FFAQSSFASY ALARENNAIK VPKEAPLELL GPLGCGIQTG AGAVINSLAV RTGSSFASFG AGAVGMSAVM AARIAGATTI IAVDIVPSRL ALALELGATH AINSKEVDVV DAIREITGGG VDYALESTGL PAVLSQGIDA LGSRGTMGVV GAPKLGTKAE FDVNSLLLGG HTIRGIVEGD SVPQTFIPQL VQLHLQGRFP FDRLVKFYPL EQINQAAADS SSGITL
Validated NT Sequence
tttgagcgtg atgccgctgc tgctgtccgc ggcggcctgg ttgatctgct cgagcggata gaacttcacc agccggtcga aggggaaccg gccttgcaga tgcagctgca cgagttgcgg aatgaaggtc tgcggcacgc tgtcgccttc gacgatcccg cggatcgtgt ggccgccgag cagcaggctg ttcacgtcga attcggcctt cgtgccgagc ttcggcgcgc cgaccacgcc catcgtgccg cgcgagccga gcgcgtcgat cccttgcgac agcacggccg gcagcccggt cgattcgagc gcgtagtcga cgccgccgcc ggtgatctcg cggatcgcgt cgacgacgtc gacttccttg ctgttgatcg cgtgcgtcgc gccgagctcc agcgccagcg cgagccgcga cggcacgatg tcgacggcga tgatggtcgt cgcgccggcg atacgcgcgg ccatcacggc gctcatcccg accgcgccgg cgccgaagct cgcgaagctg ctgcccgtgc gtaccgcgag cgaattgatt accgcgcccg cgccggtctg gatgccgcag ccgagcgggc cgagcagttc gagcggcgct tccttcggca ccttgatcgc gttgttctcg cgcgcgagcg cgtagctcgc gaacgacgac tgcgcgaaga aatgatcgtg cagcggttcg ccggctgcgt cgcggagggc cgtctgtccg tcggcatcgg caccgccgaa gttcagcgcg aagaactgcc ggcaatacgc accgtgaccg ccggcgcacg acgcgcagtg gccgcacgcg ccgtaggtga gcacgacgtg atcgccggcg gcgagcgtct tcacgttcgg gccgacggcc tcgaccacgc cggcgccttc atgaccgagc acggccggta gcggcacagg atagtactgg tcgcgcacga tcaggtcggt gtggcacagg cccgtcgcga cgacgcgcac cagcacctcg tcgccgcgcg gcgcgcggat ccgggccggt tcgatcgaga agggcgcgcc ggcggcgcgg gcgacggccg cggtgacggc gcgcgtatcg ttttcggtat acatatggtg gtggtggtgg tggtgagcca tggtatat
Expected Protein Sequence
MAHHHHHHMY TENDTRAVTA AVARAAGAPF SIEPARIRAP RGDEVLVRVV ATGLCHTDLI VRDQYYPVPL PAVLGHEGAG VVEAVGPNVK TLAAGDHVVL TYGACGHCAS CAGGHGAYCR QFFALNFGGA DADGQTALRD AAGEPLHDHF FAQSSFASYA LARENNAIKV PKEAPLELLG PLGCGIQTGA GAVINSLAVR TGSSFASFGA GAVGMSAVMA ARIAGATTII AVDIVPSRLA LALELGATHA INSKEVDVVD AIREITGGGV DYALESTGLP AVLSQGIDAL GSRGTMGVVG APKLGTKAEF DVNSLLLGGH TIRGIVEGDS VPQTFIPQLV QLHLQGRFPF DRLVKFYPLE QINQAAADSS SGITLKPILR LPH
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtata ccgaaaacga tacgcgcgcc gtcaccgcgg ccgtcgcccg cgccgccggc gcgcccttct cgatcgaacc ggcccggatc cgcgcgccgc gcggcgacga ggtgctggtg cgcgtcgtcg cgacgggcct gtgccacacc gacctgatcg tgcgcgacca gtactatcct gtgccgctac cggccgtgct cggtcatgaa ggcgccggcg tggtcgaggc cgtcggcccg aacgtgaaga cgctcgccgc cggcgatcac gtcgtgctca cctacggcgc gtgcggccac tgcgcgtcgt gcgccggcgg tcacggtgcg tattgccggc agttcttcgc gctgaacttc ggcggtgccg atgccgacgg acagacggcc ctccgcgacg cagccggcga accgctgcac gatcatttct tcgcgcagtc gtcgttcgcg agctacgcgc tcgcgcgcga gaacaacgcg atcaaggtgc cgaaggaagc gccgctcgaa ctgctcggcc cgctcggctg cggcatccag accggcgcgg gcgcggtaat caattcgctc gcggtacgca cgggcagcag cttcgcgagc ttcggcgccg gcgcggtcgg gatgagcgcc gtgatggccg cgcgtatcgc cggcgcgacg accatcatcg ccgtcgacat cgtgccgtcg cggctcgcgc tggcgctgga gctcggcgcg acgcacgcga tcaacagcaa ggaagtcgac gtcgtcgacg cgatccgcga gatcaccggc ggcggcgtcg actacgcgct cgaatcgacc gggctgccgg ccgtgctgtc gcaagggatc gacgcgctcg gctcgcgcgg cacgatgggc gtggtcggcg cgccgaagct cggcacgaag gccgaattcg acgtgaacag cctgctgctc ggcggccaca cgatccgcgg gatcgtcgaa ggcgacagcg tgccgcagac cttcattccg caactcgtgc agctgcatct gcaaggccgg ttccccttcg accggctggt gaagttctat ccgctcgagc agatcaacca ggccgccgcg gacagcagca gcggcatcac gctcaaaccg atcctgcggt tgccgcattg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for BuamA.10611.c.B1.PS37979
| PURIFICATION DATe: | 7/14/2016 |
| CONCENTRATION: | 38.21mg/ml |
| OBSERVED MW: | 38kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | SEC BUFFER |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 7 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 98 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHHM YTENDTRAVT AAVARAAGAP FSIEPARIRA PRGDEVLVRV VATGLCHTDL IVRDQYYPVP LPAVLGHEGA GVVEAVGPNV KTLAAGDHVV LTYGACGHCA SCAGGHGAYC RQFFALNFGG ADADGQTALR DAAGEPLHDH FFAQSSFASY ALARENNAIK VPKEAPLELL GPLGCGIQTG AGAVINSLAV RTGSSFASFG AGAVGMSAVM AARIAGATTI IAVDIVPSRL ALALELGATH AINSKEVDVV DAIREITGGG VDYALESTGL PAVLSQGIDA LGSRGTMGVV GAPKLGTKAE FDVNSLLLGG HTIRGIVEGD SVPQTFIPQL VQLHLQGRFP FDRLVKFYPL EQINQAAADS SSGITL
Validated NT Sequence
tttgagcgtg atgccgctgc tgctgtccgc ggcggcctgg ttgatctgct cgagcggata gaacttcacc agccggtcga aggggaaccg gccttgcaga tgcagctgca cgagttgcgg aatgaaggtc tgcggcacgc tgtcgccttc gacgatcccg cggatcgtgt ggccgccgag cagcaggctg ttcacgtcga attcggcctt cgtgccgagc ttcggcgcgc cgaccacgcc catcgtgccg cgcgagccga gcgcgtcgat cccttgcgac agcacggccg gcagcccggt cgattcgagc gcgtagtcga cgccgccgcc ggtgatctcg cggatcgcgt cgacgacgtc gacttccttg ctgttgatcg cgtgcgtcgc gccgagctcc agcgccagcg cgagccgcga cggcacgatg tcgacggcga tgatggtcgt cgcgccggcg atacgcgcgg ccatcacggc gctcatcccg accgcgccgg cgccgaagct cgcgaagctg ctgcccgtgc gtaccgcgag cgaattgatt accgcgcccg cgccggtctg gatgccgcag ccgagcgggc cgagcagttc gagcggcgct tccttcggca ccttgatcgc gttgttctcg cgcgcgagcg cgtagctcgc gaacgacgac tgcgcgaaga aatgatcgtg cagcggttcg ccggctgcgt cgcggagggc cgtctgtccg tcggcatcgg caccgccgaa gttcagcgcg aagaactgcc ggcaatacgc accgtgaccg ccggcgcacg acgcgcagtg gccgcacgcg ccgtaggtga gcacgacgtg atcgccggcg gcgagcgtct tcacgttcgg gccgacggcc tcgaccacgc cggcgccttc atgaccgagc acggccggta gcggcacagg atagtactgg tcgcgcacga tcaggtcggt gtggcacagg cccgtcgcga cgacgcgcac cagcacctcg tcgccgcgcg gcgcgcggat ccgggccggt tcgatcgaga agggcgcgcc ggcggcgcgg gcgacggccg cggtgacggc gcgcgtatcg ttttcggtat acatatggtg gtggtggtgg tggtgagcca tggtatat
Expressed Protein Sequence
MAHHHHHHMY TENDTRAVTA AVARAAGAPF SIEPARIRAP RGDEVLVRVV ATGLCHTDLI VRDQYYPVPL PAVLGHEGAG VVEAVGPNVK TLAAGDHVVL TYGACGHCAS CAGGHGAYCR QFFALNFGGA DADGQTALRD AAGEPLHDHF FAQSSFASYA LARENNAIKV PKEAPLELLG PLGCGIQTGA GAVINSLAVR TGSSFASFGA GAVGMSAVMA ARIAGATTII AVDIVPSRLA LALELGATHA INSKEVDVVD AIREITGGGV DYALESTGLP AVLSQGIDAL GSRGTMGVVG APKLGTKAEF DVNSLLLGGH TIRGIVEGDS VPQTFIPQLV QLHLQGRFPF DRLVKFYPLE QINQAAADSS SGITLKPILR LPH
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTATA CCGAAAACGA TACGCGCGCC GTCACCGCGG CCGTCGCCCG CGCCGCCGGC GCGCCCTTCT CGATCGAACC GGCCCGGATC CGCGCGCCGC GCGGCGACGA GGTGCTGGTG CGCGTCGTCG CGACGGGCCT GTGCCACACC GACCTGATCG TGCGCGACCA GTACTATCCT GTGCCGCTAC CGGCCGTGCT CGGTCATGAA GGCGCCGGCG TGGTCGAGGC CGTCGGCCCG AACGTGAAGA CGCTCGCCGC CGGCGATCAC GTCGTGCTCA CCTACGGCGC GTGCGGCCAC TGCGCGTCGT GCGCCGGCGG TCACGGTGCG TATTGCCGGC AGTTCTTCGC GCTGAACTTC GGCGGTGCCG ATGCCGACGG ACAGACGGCC CTCCGCGACG CAGCCGGCGA ACCGCTGCAC GATCATTTCT TCGCGCAGTC GTCGTTCGCG AGCTACGCGC TCGCGCGCGA GAACAACGCG ATCAAGGTGC CGAAGGAAGC GCCGCTCGAA CTGCTCGGCC CGCTCGGCTG CGGCATCCAG ACCGGCGCGG GCGCGGTAAT CAATTCGCTC GCGGTACGCA CGGGCAGCAG CTTCGCGAGC TTCGGCGCCG GCGCGGTCGG GATGAGCGCC GTGATGGCCG CGCGTATCGC CGGCGCGACG ACCATCATCG CCGTCGACAT CGTGCCGTCG CGGCTCGCGC TGGCGCTGGA GCTCGGCGCG ACGCACGCGA TCAACAGCAA GGAAGTCGAC GTCGTCGACG CGATCCGCGA GATCACCGGC GGCGGCGTCG ACTACGCGCT CGAATCGACC GGGCTGCCGG CCGTGCTGTC GCAAGGGATC GACGCGCTCG GCTCGCGCGG CACGATGGGC GTGGTCGGCG CGCCGAAGCT CGGCACGAAG GCCGAATTCG ACGTGAACAG CCTGCTGCTC GGCGGCCACA CGATCCGCGG GATCGTCGAA GGCGACAGCG TGCCGCAGAC CTTCATTCCG CAACTCGTGC AGCTGCATCT GCAAGGCCGG TTCCCCTTCG ACCGGCTGGT GAAGTTCTAT CCGCTCGAGC AGATCAACCA GGCCGCCGCG GACAGCAGCA GCGGCATCAC GCTCAAACCG ATCCTGCGGT TGCCGCATTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT