BuamA.10611.b

Alcohol dehydrogenase GroES domain protein

CENTER ID: BuamA.10611.b
ORGANISM: Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.10611.b.B1.GE39801 Full length( BuamA.10611.b ) 1 373
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.10611.b.B1.PS37900 Full length( BuamA.10611.b ) 1 373

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|398577.6.peg.3251
RefSeq: YP_001809832.1
UniProt: B1YXR6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRALQWHGPR DLRVVNLDTP RAGPGEVRIA VAYCGICGSD LHEYADGPHA IPVDTPHPLS GRTAPLTLGH EFCGTVVEVG TGVTTLTPGD RVAVEPEYHC HQCAYCRSGS YNLCVSMGFA GLMGDGGMAD FAVVPAYMLH RLPDDVSLEQ AAVMEPAAVA LHALRRGELR LGDTCAVFGL GPIGLLLILL AKLQGATTIV AVDVSPERLA AAARFGATHA FDARSLDAPV LRDAIREAAG GLGVDVSFEA AGLPATFEAA MQGLRKGGRL VMVGLMPHAG FDAFRAVNDE LTFTASVGYR HVYEDLLRLI GSGALDLTSI VTRTVALEDA VADGFDALLA DRAQIKILVA PPRRHARRPH TVGSIEHEPT LVV
NT Sequence
ATGCGTGCAC TTCAATGGCA CGGCCCGCGC GATCTCCGCG TGGTCAATCT CGACACGCCG CGCGCCGGGC CCGGCGAGGT CCGCATCGCG GTCGCGTATT GCGGCATCTG CGGCAGCGAC CTGCACGAAT ATGCAGACGG CCCGCACGCG ATTCCGGTCG ACACGCCGCA TCCGCTGTCC GGCCGCACCG CCCCGCTGAC GCTCGGTCAC GAATTCTGCG GCACCGTCGT CGAAGTCGGC ACGGGCGTGA CGACGCTGAC CCCGGGCGAC CGCGTCGCGG TCGAGCCCGA GTACCACTGC CATCAGTGCG CGTACTGCCG CTCGGGCTCG TACAACCTGT GCGTATCGAT GGGCTTCGCC GGCCTGATGG GCGACGGCGG GATGGCCGAC TTCGCGGTCG TGCCGGCCTA CATGCTGCAT CGGCTTCCCG ACGACGTCAG TCTCGAACAG GCCGCGGTGA TGGAGCCGGC GGCCGTCGCG CTGCATGCGC TGCGGCGCGG CGAGCTGCGG CTCGGCGACA CCTGCGCGGT GTTCGGGCTC GGGCCGATCG GCCTGCTGCT GATCCTGCTC GCGAAGCTGC AAGGCGCGAC GACCATCGTC GCGGTCGACG TGTCGCCGGA GCGGCTCGCG GCGGCCGCGC GCTTCGGCGC GACGCACGCG TTCGACGCGC GCTCGCTCGA CGCGCCGGTG CTGCGCGACG CGATCCGCGA GGCGGCGGGG GGCCTCGGCG TCGACGTGAG CTTCGAGGCG GCCGGCCTGC CGGCCACGTT CGAGGCCGCG ATGCAGGGGC TGCGCAAGGG CGGCCGCCTC GTGATGGTCG GCCTGATGCC GCATGCGGGT TTCGATGCGT TCCGCGCGGT CAACGACGAG CTGACCTTCA CGGCGAGCGT CGGCTACCGG CACGTGTACG AAGACCTGCT GCGGCTGATC GGGTCGGGCG CGCTCGATCT CACGTCGATC GTCACGCGCA CGGTCGCGCT CGAAGATGCC GTCGCCGACG GCTTCGACGC GCTGCTCGCC GATCGCGCGC AGATCAAGAT CCTGGTTGCG CCGCCGCGCC GGCATGCGCG CCGTCCTCAC ACCGTCGGGA GCATCGAGCA TGAGCCGACC CTGGTCGTT
Details for BuamA.10611.b.B1.GE39801
HARVESTED ON: 2/4/2016
SEQUENCED ON: 1/27/2016
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMR ALQWHGPRDL RVVNLDTPRA GPGEVRIAVA YCGICGSDLH EYADGPHAIP VDTPHPLSGR TAPLTLGHEF CGTVVEVGTG VTTLTPGDRV AVEPEYHCHQ CAYCRSGSYN LCVSMGFAGL MGDGGMADFA VVPAYMLHRL PDDVSLEQAA VMEPAAVALH ALRRGELRLG DTCAVFGLGP IGLLLILLAK LQGATTIVAV DVSPERLAAA ARFGATHAFD ARSLDAPVLR DAIREAAGGL GVDVSFEAAG LPATFEAAMQ GLRKGGRLVM VGLMPHAGFD AFRAVNDELT FTASVGYRHV YEDLLRLIGS GALDLTSIVT RTVALEDAVA DGFDALLADR AQIKILVAPP RRHARRPHTV GS
Validated NT Sequence
atgctcccga cggtgtgagg acggcgcgca tgccggcgcg gcggcgcaac caggatcttg atctgcgcgc gatcggcgag cagcgcgtcg aagccgtcgg cgacggcatc ttcgagcgcg accgtgcgcg tgacgatcga cgtgagatcg agcgcgcccg acccgatcag ccgcagcagg tcttcgtaca cgtgccggta gccgacgctc gccgtgaagg tcagctcgtc gttgaccgcg cggaacgcat cgaaacccgc atgcggcatc aggccgacca tcacgaggcg gccgcccttg cgcagcccct gcatcgcggc ctcgaacgtg gccggcaggc cggccgcctc gaagctcacg tcgacgccga ggccccccgc cgcctcgcgg atcgcgtcgc gcagcaccgg cgcgtcgagc gagcgcgcgt cgaacgcgtg cgtcgcgccg aagcgcgcgg ccgccgcgag ccgctccggc gacacgtcga ccgcgacgat ggtcgtcgcg ccttgcagct tcgcgagcag gatcagcagc aggccgatcg gcccgagccc gaacaccgcg caggtgtcgc cgagccgcag ctcgccgcgc cgcagcgcat gcagcgcgac ggccgccggc tccatcaccg cggcctgttc gagactgacg tcgtcgggaa gccgatgcag catgtaggcc ggcacgaccg cgaagtcggc catcccgccg tcgcccatca ggccggcgaa gcccatcgat acgcacaggt tgtacgagcc cgagcggcag tacgcgcact gatggcagtg gtactcgggc tcgaccgcga cgcggtcgcc cggggtcagc gtcgtcacgc ccgtgccgac ttcgacgacg gtgccgcaga attcgtgacc gagcgtcagc ggggcggtgc ggccggacag cggatgcggc gtgtcgaccg gaatcgcgtg cgggccgtct gcatattcgt gcaggtcgct gccgcagatg ccgcaatacg cgaccgcgat gcggacctcg ccgggcccgg cgcgcggcgt gtcgagattg accacgcgga gatcgcgcgg gccgtgccat tgaagtgcac gcatatggtg gtggtggtgg tgagccatgg tatat
Expected Protein Sequence
MAHHHHHHMR ALQWHGPRDL RVVNLDTPRA GPGEVRIAVA YCGICGSDLH EYADGPHAIP VDTPHPLSGR TAPLTLGHEF CGTVVEVGTG VTTLTPGDRV AVEPEYHCHQ CAYCRSGSYN LCVSMGFAGL MGDGGMADFA VVPAYMLHRL PDDVSLEQAA VMEPAAVALH ALRRGELRLG DTCAVFGLGP IGLLLILLAK LQGATTIVAV DVSPERLAAA ARFGATHAFD ARSLDAPVLR DAIREAAGGL GVDVSFEAAG LPATFEAAMQ GLRKGGRLVM VGLMPHAGFD AFRAVNDELT FTASVGYRHV YEDLLRLIGS GALDLTSIVT RTVALEDAVA DGFDALLADR AQIKILVAPP RRHARRPHTV GSIEHEPTLV V
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgtg cacttcaatg gcacggcccg cgcgatctcc gcgtggtcaa tctcgacacg ccgcgcgccg ggcccggcga ggtccgcatc gcggtcgcgt attgcggcat ctgcggcagc gacctgcacg aatatgcaga cggcccgcac gcgattccgg tcgacacgcc gcatccgctg tccggccgca ccgccccgct gacgctcggt cacgaattct gcggcaccgt cgtcgaagtc ggcacgggcg tgacgacgct gaccccgggc gaccgcgtcg cggtcgagcc cgagtaccac tgccatcagt gcgcgtactg ccgctcgggc tcgtacaacc tgtgcgtatc gatgggcttc gccggcctga tgggcgacgg cgggatggcc gacttcgcgg tcgtgccggc ctacatgctg catcggcttc ccgacgacgt cagtctcgaa caggccgcgg tgatggagcc ggcggccgtc gcgctgcatg cgctgcggcg cggcgagctg cggctcggcg acacctgcgc ggtgttcggg ctcgggccga tcggcctgct gctgatcctg ctcgcgaagc tgcaaggcgc gacgaccatc gtcgcggtcg acgtgtcgcc ggagcggctc gcggcggccg cgcgcttcgg cgcgacgcac gcgttcgacg cgcgctcgct cgacgcgccg gtgctgcgcg acgcgatccg cgaggcggcg gggggcctcg gcgtcgacgt gagcttcgag gcggccggcc tgccggccac gttcgaggcc gcgatgcagg ggctgcgcaa gggcggccgc ctcgtgatgg tcggcctgat gccgcatgcg ggtttcgatg cgttccgcgc ggtcaacgac gagctgacct tcacggcgag cgtcggctac cggcacgtgt acgaagacct gctgcggctg atcgggtcgg gcgcgctcga tctcacgtcg atcgtcacgc gcacggtcgc gctcgaagat gccgtcgccg acggcttcga cgcgctgctc gccgatcgcg cgcagatcaa gatcctggtt gcgccgccgc gccggcatgc gcgccgtcct cacaccgtcg ggagcatcga gcatgagccg accctggtcg tttgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for BuamA.10611.b.B1.PS37900
PURIFICATION DATe: 4/6/2016
CONCENTRATION: 20.2mg/ml
OBSERVED MW: 40kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: SEC Buffer
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMR ALQWHGPRDL RVVNLDTPRA GPGEVRIAVA YCGICGSDLH EYADGPHAIP VDTPHPLSGR TAPLTLGHEF CGTVVEVGTG VTTLTPGDRV AVEPEYHCHQ CAYCRSGSYN LCVSMGFAGL MGDGGMADFA VVPAYMLHRL PDDVSLEQAA VMEPAAVALH ALRRGELRLG DTCAVFGLGP IGLLLILLAK LQGATTIVAV DVSPERLAAA ARFGATHAFD ARSLDAPVLR DAIREAAGGL GVDVSFEAAG LPATFEAAMQ GLRKGGRLVM VGLMPHAGFD AFRAVNDELT FTASVGYRHV YEDLLRLIGS GALDLTSIVT RTVALEDAVA DGFDALLADR AQIKILVAPP RRHARRPHTV GS
Validated NT Sequence
atgctcccga cggtgtgagg acggcgcgca tgccggcgcg gcggcgcaac caggatcttg atctgcgcgc gatcggcgag cagcgcgtcg aagccgtcgg cgacggcatc ttcgagcgcg accgtgcgcg tgacgatcga cgtgagatcg agcgcgcccg acccgatcag ccgcagcagg tcttcgtaca cgtgccggta gccgacgctc gccgtgaagg tcagctcgtc gttgaccgcg cggaacgcat cgaaacccgc atgcggcatc aggccgacca tcacgaggcg gccgcccttg cgcagcccct gcatcgcggc ctcgaacgtg gccggcaggc cggccgcctc gaagctcacg tcgacgccga ggccccccgc cgcctcgcgg atcgcgtcgc gcagcaccgg cgcgtcgagc gagcgcgcgt cgaacgcgtg cgtcgcgccg aagcgcgcgg ccgccgcgag ccgctccggc gacacgtcga ccgcgacgat ggtcgtcgcg ccttgcagct tcgcgagcag gatcagcagc aggccgatcg gcccgagccc gaacaccgcg caggtgtcgc cgagccgcag ctcgccgcgc cgcagcgcat gcagcgcgac ggccgccggc tccatcaccg cggcctgttc gagactgacg tcgtcgggaa gccgatgcag catgtaggcc ggcacgaccg cgaagtcggc catcccgccg tcgcccatca ggccggcgaa gcccatcgat acgcacaggt tgtacgagcc cgagcggcag tacgcgcact gatggcagtg gtactcgggc tcgaccgcga cgcggtcgcc cggggtcagc gtcgtcacgc ccgtgccgac ttcgacgacg gtgccgcaga attcgtgacc gagcgtcagc ggggcggtgc ggccggacag cggatgcggc gtgtcgaccg gaatcgcgtg cgggccgtct gcatattcgt gcaggtcgct gccgcagatg ccgcaatacg cgaccgcgat gcggacctcg ccgggcccgg cgcgcggcgt gtcgagattg accacgcgga gatcgcgcgg gccgtgccat tgaagtgcac gcatatggtg gtggtggtgg tgagccatgg tatat
Expressed Protein Sequence
MAHHHHHHMR ALQWHGPRDL RVVNLDTPRA GPGEVRIAVA YCGICGSDLH EYADGPHAIP VDTPHPLSGR TAPLTLGHEF CGTVVEVGTG VTTLTPGDRV AVEPEYHCHQ CAYCRSGSYN LCVSMGFAGL MGDGGMADFA VVPAYMLHRL PDDVSLEQAA VMEPAAVALH ALRRGELRLG DTCAVFGLGP IGLLLILLAK LQGATTIVAV DVSPERLAAA ARFGATHAFD ARSLDAPVLR DAIREAAGGL GVDVSFEAAG LPATFEAAMQ GLRKGGRLVM VGLMPHAGFD AFRAVNDELT FTASVGYRHV YEDLLRLIGS GALDLTSIVT RTVALEDAVA DGFDALLADR AQIKILVAPP RRHARRPHTV GSIEHEPTLV V
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCGTG CACTTCAATG GCACGGCCCG CGCGATCTCC GCGTGGTCAA TCTCGACACG CCGCGCGCCG GGCCCGGCGA GGTCCGCATC GCGGTCGCGT ATTGCGGCAT CTGCGGCAGC GACCTGCACG AATATGCAGA CGGCCCGCAC GCGATTCCGG TCGACACGCC GCATCCGCTG TCCGGCCGCA CCGCCCCGCT GACGCTCGGT CACGAATTCT GCGGCACCGT CGTCGAAGTC GGCACGGGCG TGACGACGCT GACCCCGGGC GACCGCGTCG CGGTCGAGCC CGAGTACCAC TGCCATCAGT GCGCGTACTG CCGCTCGGGC TCGTACAACC TGTGCGTATC GATGGGCTTC GCCGGCCTGA TGGGCGACGG CGGGATGGCC GACTTCGCGG TCGTGCCGGC CTACATGCTG CATCGGCTTC CCGACGACGT CAGTCTCGAA CAGGCCGCGG TGATGGAGCC GGCGGCCGTC GCGCTGCATG CGCTGCGGCG CGGCGAGCTG CGGCTCGGCG ACACCTGCGC GGTGTTCGGG CTCGGGCCGA TCGGCCTGCT GCTGATCCTG CTCGCGAAGC TGCAAGGCGC GACGACCATC GTCGCGGTCG ACGTGTCGCC GGAGCGGCTC GCGGCGGCCG CGCGCTTCGG CGCGACGCAC GCGTTCGACG CGCGCTCGCT CGACGCGCCG GTGCTGCGCG ACGCGATCCG CGAGGCGGCG GGGGGCCTCG GCGTCGACGT GAGCTTCGAG GCGGCCGGCC TGCCGGCCAC GTTCGAGGCC GCGATGCAGG GGCTGCGCAA GGGCGGCCGC CTCGTGATGG TCGGCCTGAT GCCGCATGCG GGTTTCGATG CGTTCCGCGC GGTCAACGAC GAGCTGACCT TCACGGCGAG CGTCGGCTAC CGGCACGTGT ACGAAGACCT GCTGCGGCTG ATCGGGTCGG GCGCGCTCGA TCTCACGTCG ATCGTCACGC GCACGGTCGC GCTCGAAGAT GCCGTCGCCG ACGGCTTCGA CGCGCTGCTC GCCGATCGCG CGCAGATCAA GATCCTGGTT GCGCCGCCGC GCCGGCATGC GCGCCGTCCT CACACCGTCG GGAGCATCGA GCATGAGCCG ACCCTGGTCG TTTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT