BuamA.10611.a

Alcohol dehydrogenase GroES domain protein

CENTER ID: BuamA.10611.a
ORGANISM: Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.10611.a.B1.GE39800 Full length( BuamA.10611.a ) 1 335
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.10611.a.B1.PS37901 Full length( BuamA.10611.a ) 1 335

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|398577.6.peg.1278
RefSeq: YP_001807956.1
UniProt: B1YNC2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLSIICESPG ILRHEDRAPP SRAEGEILLR VQRVGICGTD MHIYSGNQPY LQYPRVMGHE LSAIVAEADP GAHVAPGDAV YVMPYLSCGQ CIACRQGKTN CCVNIKVLGV HRDGALTEYL SVPAQFVHKA EGVTLDQAAM IEFLAIGAHA VRRAAVSAGQ RILVVGAGPI GMAAMLFAQL RGAHVTCLDT RADRLAFCRE HFAVATVQTG PNDVDELSTM TNGEFFDVVF DATGNVKAMN RGFEFIAHGG KYVLISIVPG EITFSDPEFH KREATLLGSR NATAEDFETV LDAMRAGRIP DKALNTHRMS LSDVPVEFPK LMELGQTVVK ALVEC
NT Sequence
ATGCTCAGCA TCATTTGCGA ATCGCCCGGC ATACTTCGCC ACGAAGATCG CGCGCCGCCT TCCCGCGCCG AAGGGGAGAT ATTGTTGCGC GTGCAACGAG TCGGTATTTG CGGCACCGAC ATGCACATCT ATTCGGGCAA TCAACCCTAC CTGCAATATC CGCGCGTCAT GGGCCATGAA CTGTCCGCCA TCGTCGCCGA GGCCGATCCC GGCGCGCATG TCGCGCCTGG AGACGCGGTC TACGTGATGC CCTACCTGTC GTGCGGACAG TGCATCGCGT GCCGACAGGG CAAGACCAAC TGCTGCGTCA ACATCAAGGT GCTCGGGGTG CATCGTGACG GCGCATTGAC CGAATATCTG TCGGTGCCCG CGCAGTTCGT GCACAAGGCC GAAGGGGTCA CGCTTGACCA GGCGGCGATG ATCGAATTCC TGGCCATCGG CGCGCACGCG GTGCGGCGGG CCGCCGTGTC CGCAGGCCAA AGGATCCTCG TGGTGGGTGC GGGGCCGATC GGCATGGCGG CGATGCTGTT CGCCCAGCTG AGAGGCGCAC ACGTCACCTG TCTGGACACA CGCGCCGATC GCCTTGCGTT CTGCCGCGAG CACTTCGCCG TTGCGACGGT GCAGACCGGG CCGAACGACG TCGACGAATT GTCGACGATG ACCAACGGCG AGTTTTTCGA CGTTGTGTTC GATGCGACGG GTAACGTCAA GGCCATGAAT CGCGGTTTCG AGTTCATTGC GCACGGCGGC AAGTACGTGC TGATTTCGAT TGTCCCGGGC GAGATCACAT TCTCCGATCC GGAGTTTCAC AAGCGCGAAG CAACGCTGCT CGGCAGCCGC AACGCCACCG CCGAAGACTT CGAGACCGTC CTCGACGCGA TGCGCGCCGG CCGCATTCCG GACAAGGCGC TCAATACGCA TCGGATGAGC CTGTCGGACG TTCCCGTCGA GTTCCCTAAA CTGATGGAAC TCGGGCAGAC CGTCGTCAAG GCGCTGGTCG AGTGC
Details for BuamA.10611.a.B1.GE39800
HARVESTED ON: 2/4/2016
SEQUENCED ON: 1/27/2016
EXPECTED MW: 37kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHML SIICESPGIL RHEDRAPPSR AEGEILLRVQ RVGICGTDMH IYSGNQPYLQ YPRVMGHELS AIVAEADPGA HVAPGDAVYV MPYLSCGQCI ACRQGKTNCC VNIKVLGVHR DGALTEYLSV PAQFVHKAEG VTLDQAAMIE FLAIGAHAVR RAAVSAGQRI LVVGAGPIGM AAMLFAQLRG AHVTCLDTRA DRLAFCREHF AVATVQTGPN DVDELSTMTN GEFFDVVFDA TGNVKAMNRG FEFIAHGGKY VLISIVPGEI TFSDPEFHKR EATLLGSRNA TAEDFETVLD AMRAGRIPDK ALNTHRMSLS DVPVEFPKLM ELGQ
Validated NT Sequence
gtctgcccga gttccatcag tttagggaac tcgacgggaa cgtccgacag gctcatccga tgcgtattga gcgccttgtc cggaatgcgg ccggcgcgca tcgcgtcgag gacggtctcg aagtcttcgg cggtggcgtt gcggctgccg agcagcgttg cttcgcgctt gtgaaactcc ggatcggaga atgtgatctc gcccgggaca atcgaaatca gcacgtactt gccgccgtgc gcaatgaact cgaaaccgcg attcatggcc ttgacgttac ccgtcgcatc gaacacaacg tcgaaaaact cgccgttggt catcgtcgac aattcgtcga cgtcgttcgg cccggtctgc accgtcgcaa cggcgaagtg ctcgcggcag aacgcaaggc gatcggcgcg tgtgtccaga caggtgacgt gtgcgcctct cagctgggcg aacagcatcg ccgccatgcc gatcggcccc gcacccacca cgaggatcct ttggcctgcg gacacggcgg cccgccgcac cgcgtgcgcg ccgatggcca ggaattcgat catcgccgcc tggtcaagcg tgaccccttc ggccttgtgc acgaactgcg cgggcaccga cagatattcg gtcaatgcgc cgtcacgatg caccccgagc accttgatgt tgacgcagca gttggtcttg ccctgtcggc acgcgatgca ctgtccgcac gacaggtagg gcatcacgta gaccgcgtct ccaggcgcga catgcgcgcc gggatcggcc tcggcgacga tggcggacag ttcatggccc atgacgcgcg gatattgcag gtagggttga ttgcccgaat agatgtgcat gtcggtgccg caaataccga ctcgttgcac gcgcaacaat atctcccctt cggcgcggga aggcggcgcg cgatcttcgt ggcgaagtat gccgggcgat tcgcaaatga tgctgagcat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHML SIICESPGIL RHEDRAPPSR AEGEILLRVQ RVGICGTDMH IYSGNQPYLQ YPRVMGHELS AIVAEADPGA HVAPGDAVYV MPYLSCGQCI ACRQGKTNCC VNIKVLGVHR DGALTEYLSV PAQFVHKAEG VTLDQAAMIE FLAIGAHAVR RAAVSAGQRI LVVGAGPIGM AAMLFAQLRG AHVTCLDTRA DRLAFCREHF AVATVQTGPN DVDELSTMTN GEFFDVVFDA TGNVKAMNRG FEFIAHGGKY VLISIVPGEI TFSDPEFHKR EATLLGSRNA TAEDFETVLD AMRAGRIPDK ALNTHRMSLS DVPVEFPKLM ELGQTVVKAL VEC
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctca gcatcatttg cgaatcgccc ggcatacttc gccacgaaga tcgcgcgccg ccttcccgcg ccgaagggga gatattgttg cgcgtgcaac gagtcggtat ttgcggcacc gacatgcaca tctattcggg caatcaaccc tacctgcaat atccgcgcgt catgggccat gaactgtccg ccatcgtcgc cgaggccgat cccggcgcgc atgtcgcgcc tggagacgcg gtctacgtga tgccctacct gtcgtgcgga cagtgcatcg cgtgccgaca gggcaagacc aactgctgcg tcaacatcaa ggtgctcggg gtgcatcgtg acggcgcatt gaccgaatat ctgtcggtgc ccgcgcagtt cgtgcacaag gccgaagggg tcacgcttga ccaggcggcg atgatcgaat tcctggccat cggcgcgcac gcggtgcggc gggccgccgt gtccgcaggc caaaggatcc tcgtggtggg tgcggggccg atcggcatgg cggcgatgct gttcgcccag ctgagaggcg cacacgtcac ctgtctggac acacgcgccg atcgccttgc gttctgccgc gagcacttcg ccgttgcgac ggtgcagacc gggccgaacg acgtcgacga attgtcgacg atgaccaacg gcgagttttt cgacgttgtg ttcgatgcga cgggtaacgt caaggccatg aatcgcggtt tcgagttcat tgcgcacggc ggcaagtacg tgctgatttc gattgtcccg ggcgagatca cattctccga tccggagttt cacaagcgcg aagcaacgct gctcggcagc cgcaacgcca ccgccgaaga cttcgagacc gtcctcgacg cgatgcgcgc cggccgcatt ccggacaagg cgctcaatac gcatcggatg agcctgtcgg acgttcccgt cgagttccct aaactgatgg aactcgggca gaccgtcgtc aaggcgctgg tcgagtgctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for BuamA.10611.a.B1.PS37901
PURIFICATION DATe: 4/6/2016
CONCENTRATION: 27.6mg/ml
OBSERVED MW: 38kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: SEC
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHML SIICESPGIL RHEDRAPPSR AEGEILLRVQ RVGICGTDMH IYSGNQPYLQ YPRVMGHELS AIVAEADPGA HVAPGDAVYV MPYLSCGQCI ACRQGKTNCC VNIKVLGVHR DGALTEYLSV PAQFVHKAEG VTLDQAAMIE FLAIGAHAVR RAAVSAGQRI LVVGAGPIGM AAMLFAQLRG AHVTCLDTRA DRLAFCREHF AVATVQTGPN DVDELSTMTN GEFFDVVFDA TGNVKAMNRG FEFIAHGGKY VLISIVPGEI TFSDPEFHKR EATLLGSRNA TAEDFETVLD AMRAGRIPDK ALNTHRMSLS DVPVEFPKLM ELGQ
Validated NT Sequence
gtctgcccga gttccatcag tttagggaac tcgacgggaa cgtccgacag gctcatccga tgcgtattga gcgccttgtc cggaatgcgg ccggcgcgca tcgcgtcgag gacggtctcg aagtcttcgg cggtggcgtt gcggctgccg agcagcgttg cttcgcgctt gtgaaactcc ggatcggaga atgtgatctc gcccgggaca atcgaaatca gcacgtactt gccgccgtgc gcaatgaact cgaaaccgcg attcatggcc ttgacgttac ccgtcgcatc gaacacaacg tcgaaaaact cgccgttggt catcgtcgac aattcgtcga cgtcgttcgg cccggtctgc accgtcgcaa cggcgaagtg ctcgcggcag aacgcaaggc gatcggcgcg tgtgtccaga caggtgacgt gtgcgcctct cagctgggcg aacagcatcg ccgccatgcc gatcggcccc gcacccacca cgaggatcct ttggcctgcg gacacggcgg cccgccgcac cgcgtgcgcg ccgatggcca ggaattcgat catcgccgcc tggtcaagcg tgaccccttc ggccttgtgc acgaactgcg cgggcaccga cagatattcg gtcaatgcgc cgtcacgatg caccccgagc accttgatgt tgacgcagca gttggtcttg ccctgtcggc acgcgatgca ctgtccgcac gacaggtagg gcatcacgta gaccgcgtct ccaggcgcga catgcgcgcc gggatcggcc tcggcgacga tggcggacag ttcatggccc atgacgcgcg gatattgcag gtagggttga ttgcccgaat agatgtgcat gtcggtgccg caaataccga ctcgttgcac gcgcaacaat atctcccctt cggcgcggga aggcggcgcg cgatcttcgt ggcgaagtat gccgggcgat tcgcaaatga tgctgagcat atggtggtgg tggtggtgag ccat
Expressed Protein Sequence
MAHHHHHHML SIICESPGIL RHEDRAPPSR AEGEILLRVQ RVGICGTDMH IYSGNQPYLQ YPRVMGHELS AIVAEADPGA HVAPGDAVYV MPYLSCGQCI ACRQGKTNCC VNIKVLGVHR DGALTEYLSV PAQFVHKAEG VTLDQAAMIE FLAIGAHAVR RAAVSAGQRI LVVGAGPIGM AAMLFAQLRG AHVTCLDTRA DRLAFCREHF AVATVQTGPN DVDELSTMTN GEFFDVVFDA TGNVKAMNRG FEFIAHGGKY VLISIVPGEI TFSDPEFHKR EATLLGSRNA TAEDFETVLD AMRAGRIPDK ALNTHRMSLS DVPVEFPKLM ELGQTVVKAL VEC
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCTCA GCATCATTTG CGAATCGCCC GGCATACTTC GCCACGAAGA TCGCGCGCCG CCTTCCCGCG CCGAAGGGGA GATATTGTTG CGCGTGCAAC GAGTCGGTAT TTGCGGCACC GACATGCACA TCTATTCGGG CAATCAACCC TACCTGCAAT ATCCGCGCGT CATGGGCCAT GAACTGTCCG CCATCGTCGC CGAGGCCGAT CCCGGCGCGC ATGTCGCGCC TGGAGACGCG GTCTACGTGA TGCCCTACCT GTCGTGCGGA CAGTGCATCG CGTGCCGACA GGGCAAGACC AACTGCTGCG TCAACATCAA GGTGCTCGGG GTGCATCGTG ACGGCGCATT GACCGAATAT CTGTCGGTGC CCGCGCAGTT CGTGCACAAG GCCGAAGGGG TCACGCTTGA CCAGGCGGCG ATGATCGAAT TCCTGGCCAT CGGCGCGCAC GCGGTGCGGC GGGCCGCCGT GTCCGCAGGC CAAAGGATCC TCGTGGTGGG TGCGGGGCCG ATCGGCATGG CGGCGATGCT GTTCGCCCAG CTGAGAGGCG CACACGTCAC CTGTCTGGAC ACACGCGCCG ATCGCCTTGC GTTCTGCCGC GAGCACTTCG CCGTTGCGAC GGTGCAGACC GGGCCGAACG ACGTCGACGA ATTGTCGACG ATGACCAACG GCGAGTTTTT CGACGTTGTG TTCGATGCGA CGGGTAACGT CAAGGCCATG AATCGCGGTT TCGAGTTCAT TGCGCACGGC GGCAAGTACG TGCTGATTTC GATTGTCCCG GGCGAGATCA CATTCTCCGA TCCGGAGTTT CACAAGCGCG AAGCAACGCT GCTCGGCAGC CGCAACGCCA CCGCCGAAGA CTTCGAGACC GTCCTCGACG CGATGCGCGC CGGCCGCATT CCGGACAAGG CGCTCAATAC GCATCGGATG AGCCTGTCGG ACGTTCCCGT CGAGTTCCCT AAACTGATGG AACTCGGGCA GACCGTCGTC AAGGCGCTGG TCGAGTGCTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT