BuamA.10100.c

Peptidase S1 and S6 chymotrypsin/Hap

CENTER ID: BuamA.10100.c
ORGANISM: Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.10100.c.B1.GE39798 Full length( BuamA.10100.c ) 1 584
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|398577.6.peg.4568
RefSeq: YP_001811082.1
UniProt: B1YX04

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRVSESYMAD AAWTINGCVY RRLPGVRVSR ARRRRDCRGR CTVVRQTLAR AVLRTALIGG VFAGILSSSP PTRAGTVATP APPPVMKSRP AARAYSMPVD FPAIVDRYGP AVVTIMTTGP DQQPGGPAFA ILEPDDPLAG FFRRGAQPPA PPPPLMPGQG PQAPLPDTSP RAIAGSGSGF IVSPDGLILT SAHVVADATD VTVRLADRRD LKATVLAVDP QSDVAVLRVD ATKLPFVRIG DSARVRAGEP VMTIGAPDGS GNTVTAGIVS ATAHPLPDGS AFPFFQTDIA VNPDNSGGPV FNRAGEVIGI AVQVYTGADR YASTTFAIPI AFAMKVRAQA QAQMRTNAQA QRASSGNALG VDVQDVGTGL AAAFGLPRPV GALVNGVDPG SPAAAAGVKP GDVIVQFGDK PIGQAAELND LAAALPPGGK VPLRLIRNRT PMRLTVTGAA TGDAIDNATY NATYNAPYNA PATGSAGMVG TAGAAGVAMA AARPADGGDR LGLTMHPLSD AERRSTGLAV GMMVDAVHGP AARAGIRPGD VVLELNDTLL ETPEDVPALE ASGGVIAVLI QRENARKFVS VRAR
NT Sequence
ATGCGGGTTT CCGAATCGTA TATGGCCGAC GCGGCGTGGA CTATAAATGG ATGCGTGTAC CGGCGCCTGC CGGGCGTCCG CGTGTCTCGC GCGAGACGTC GCCGCGACTG CCGGGGGAGG TGCACCGTGG TTCGCCAGAC GCTTGCTCGC GCCGTGCTTC GCACCGCGTT GATCGGGGGC GTATTCGCCG GCATTTTGTC GTCGTCGCCG CCAACCCGTG CGGGGACCGT CGCGACGCCC GCCCCACCGC CTGTCATGAA AAGCAGGCCC GCCGCCCGCG CTTACTCGAT GCCGGTCGAC TTTCCGGCGA TCGTCGACCG CTACGGCCCG GCGGTCGTGA CCATCATGAC CACCGGGCCC GACCAGCAGC CCGGTGGCCC CGCCTTCGCA ATCCTCGAAC CCGACGATCC GCTCGCCGGG TTCTTCCGCC GCGGCGCGCA GCCGCCTGCG CCGCCACCTC CGCTCATGCC CGGGCAGGGC CCGCAGGCGC CGTTGCCCGA TACGTCGCCG CGCGCGATCG CGGGCAGCGG CTCGGGCTTC ATCGTCAGCC CCGACGGGCT GATCCTGACG TCCGCGCATG TCGTCGCCGA CGCGACCGAC GTCACGGTGC GACTGGCCGA TCGTCGCGAC CTGAAGGCGA CGGTGCTGGC GGTCGATCCG CAGAGCGACG TCGCGGTGCT GCGCGTCGAC GCGACGAAGC TGCCGTTCGT GCGCATCGGC GATTCGGCGC GGGTGCGCGC CGGCGAACCG GTGATGACGA TCGGCGCCCC CGACGGCTCG GGCAATACGG TGACGGCCGG CATCGTCAGC GCGACCGCGC ATCCGCTGCC GGACGGCAGC GCGTTTCCGT TCTTCCAGAC CGACATCGCG GTGAATCCCG ACAACTCCGG CGGCCCGGTG TTCAACCGTG CGGGCGAGGT GATCGGCATC GCGGTGCAGG TCTACACCGG CGCCGACCGC TACGCGAGCA CGACGTTCGC GATCCCGATC GCGTTCGCCA TGAAGGTGCG CGCGCAAGCG CAGGCGCAGA TGCGGACCAA CGCACAGGCG CAGCGCGCTT CGTCCGGCAA CGCGCTGGGC GTCGACGTAC AGGATGTCGG CACCGGGTTG GCCGCCGCGT TCGGGCTGCC GCGTCCGGTC GGCGCGCTCG TCAACGGCGT CGATCCCGGC TCGCCGGCCG CCGCGGCCGG CGTGAAGCCC GGCGACGTGA TCGTGCAGTT CGGCGACAAG CCGATCGGCC AGGCTGCCGA GCTGAACGAC CTGGCCGCCG CGTTGCCGCC TGGCGGGAAG GTGCCGCTGC GGCTGATCCG CAACCGCACG CCGATGCGGC TGACGGTCAC GGGAGCGGCG ACCGGTGACG CGATCGACAA CGCGACTTAC AACGCGACTT ACAACGCGCC CTACAACGCG CCCGCGACGG GCAGCGCAGG CATGGTCGGT ACGGCCGGCG CCGCCGGCGT GGCGATGGCC GCCGCGCGGC CGGCCGATGG CGGCGATCGG CTCGGGCTGA CGATGCATCC GCTAAGCGAT GCCGAACGGC GTTCGACCGG GCTCGCGGTC GGGATGATGG TGGACGCGGT GCACGGGCCG GCCGCGCGCG CGGGCATCCG GCCGGGCGAT GTCGTGCTGG AACTCAACGA CACGCTGCTC GAAACGCCGG AAGACGTGCC GGCGCTCGAA GCGAGCGGCG GCGTGATCGC GGTGCTCATC CAGCGCGAGA ACGCGCGCAA GTTCGTGTCG GTGCGGGCGC GG
Details for BuamA.10100.c.B1.GE39798
HARVESTED ON: 2/4/2016
SEQUENCED ON: 1/27/2016
EXPECTED MW: 61kDa
OBSERVED MW: 61kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 60
PERCENT COVERAGE: 77
Validated AA Sequence
MAHHHHHHMR VSESYMADAA WTINGCVYRR LPGVRVSRAR RRRDCRGRCT VVRQTLARAV LRTALIGGVF AGILSSSPPT RAGTVATPAP PPVMKSRPAA RAYSMPVDFP AIVDRYGPAV VTIMTTGPDQ QPGGPAFAIL EPDDPLAGFF RRGAQPPAPP PPLMPGQGPQ APLPDTSPRA IAGSGSGFIV SPDGLILTSA HVVADATDVT VRXADRRDLK ATVLAVDPXX DVXXXRXXXX XXPFXXXGXX XRXXRRXXXD XXXXXRXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXPXXXXX XRAXXXXXXX XXXXTGXXXX AXXXFAXPXX VRHEGARASA GADADQRTGA ARFVRQRAGR RRTGCRHRVG RRVRAAASGR RARQRRRSRL AGRRGRREAR RRDRAVRRQA DRPGCRAERP GRRVAAWREG AAAADPQPHA DAADGHGSGD R
Validated NT Sequence
cgcgttctcg cgctggatga gcaccgcgat cacgccgccg ctcgcttcga gcgccggcac gtcttccggc gtttcgagca gcgtgtcgtt gagttccagc acgacatcgc ccggccggat gcccgcgcgc gcggccggcc cgtgcaccgc gtccaccatc atcccgaccg cgagcccggt cgaacgccgt tcggcatcgc ttagcggatg catcgtcagc ccgagccgat cgccgccatc ggccggccgc gcggcggcca tcgccacgcc ggcggcgccg gccgtaccga ccatgcctgc gctgcccgtc gcgggcgcgt tgtagggcgc gttgtaagtc gcgttgtaag tcgcgttgtc gatcgcgtca ccggtcgccg ctcccgtgac cgtcagccgc atcggcgtgc ggttgcggat cagccgcagc ggcaccttcc cgccaggcgg caacgcggcg gccaggtcgt tcagctcggc agcctggccg atcggcttgt cgccgaactg cacgatcacg tcgccgggct tcacgccggc cgcggcggcc ggcgagccgg gatcgacgcc gttgacgagc gcgccgaccg gacgcggcag cccgaacgcg gcggccaacc cggtgccgac atcctgtacg tcgacgccca gcgcgttgcc ggacgaagcg cgctgcgcct gtgcgttggt ccgcatctgc gcctgcgctt gcgcgcgcac cttcatggcg aacgnnntcg ggannngcga acnnnnnnnn ngcnnnnnng nnnnngccgg tgtnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngcacg gnnnnnnnnn nnnnnnnncg gnnntnnnnn nnnnnnnnnn nnnnnnngnn nnnnnnnnng nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngnn nnnnnnnnnn nnnnnnnnnn nnnngtcgnn nnnnnnnann nnnatcannn nnnnnncggc gcnnnnnccg cgnnnnnnnn ccnnnnnnnn cgaacggcan nnnnnnnnnn tnnncncgna nnnnnnnnac gtcgcnntgc ggatcgaccg ccagcaccgt cgccttcagg tcgcgacgat cggccnnncg caccgtgacg tcggtcgcgt cggcgacgac atgcgcggac gtcaggatca gcccgtcggg gctgacgatg aagcccgagc cgctgcccgc gatcgcgcgc ggcgacgtat cgggcaacgg cgcctgcggg ccctgcccgg gcatgagcgg aggtggcggc gcaggcggct gcgcgccgcg gcggaagaac ccggcgagcg gatcgtcggg ttcgaggatt gcgaaggcgg ggccaccggg ctgctggtcg ggcccggtgg tcatgatggt cacgaccgcc gggccgtagc ggtcgacgat cgccggaaag tcgaccggca tcgagtaagc gcgggcggcg ggcctgcttt tcatgacagg cggtggggcg ggcgtcgcga cggtccccgc acgggttggc ggcgacgacg acaaaatgcc ggcgaatacg cccccgatca acgcggtgcg aagcacggcg cgagcaagcg tctggcgaac cacggtgcac ctcccccggc agtcgcggcg acgtctcgcg cgagacacgc ggacgcccgg caggcgccgg tacacgcatc catttatagt ccacgccgcg tcggccatat acgattcgga aacccgcata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHMR VSESYMADAA WTINGCVYRR LPGVRVSRAR RRRDCRGRCT VVRQTLARAV LRTALIGGVF AGILSSSPPT RAGTVATPAP PPVMKSRPAA RAYSMPVDFP AIVDRYGPAV VTIMTTGPDQ QPGGPAFAIL EPDDPLAGFF RRGAQPPAPP PPLMPGQGPQ APLPDTSPRA IAGSGSGFIV SPDGLILTSA HVVADATDVT VRLADRRDLK ATVLAVDPQS DVAVLRVDAT KLPFVRIGDS ARVRAGEPVM TIGAPDGSGN TVTAGIVSAT AHPLPDGSAF PFFQTDIAVN PDNSGGPVFN RAGEVIGIAV QVYTGADRYA STTFAIPIAF AMKVRAQAQA QMRTNAQAQR ASSGNALGVD VQDVGTGLAA AFGLPRPVGA LVNGVDPGSP AAAAGVKPGD VIVQFGDKPI GQAAELNDLA AALPPGGKVP LRLIRNRTPM RLTVTGAATG DAIDNATYNA TYNAPYNAPA TGSAGMVGTA GAAGVAMAAA RPADGGDRLG LTMHPLSDAE RRSTGLAVGM MVDAVHGPAA RAGIRPGDVV LELNDTLLET PEDVPALEAS GGVIAVLIQR ENARKFVSVR AR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcggg tttccgaatc gtatatggcc gacgcggcgt ggactataaa tggatgcgtg taccggcgcc tgccgggcgt ccgcgtgtct cgcgcgagac gtcgccgcga ctgccggggg aggtgcaccg tggttcgcca gacgcttgct cgcgccgtgc ttcgcaccgc gttgatcggg ggcgtattcg ccggcatttt gtcgtcgtcg ccgccaaccc gtgcggggac cgtcgcgacg cccgccccac cgcctgtcat gaaaagcagg cccgccgccc gcgcttactc gatgccggtc gactttccgg cgatcgtcga ccgctacggc ccggcggtcg tgaccatcat gaccaccggg cccgaccagc agcccggtgg ccccgccttc gcaatcctcg aacccgacga tccgctcgcc gggttcttcc gccgcggcgc gcagccgcct gcgccgccac ctccgctcat gcccgggcag ggcccgcagg cgccgttgcc cgatacgtcg ccgcgcgcga tcgcgggcag cggctcgggc ttcatcgtca gccccgacgg gctgatcctg acgtccgcgc atgtcgtcgc cgacgcgacc gacgtcacgg tgcgactggc cgatcgtcgc gacctgaagg cgacggtgct ggcggtcgat ccgcagagcg acgtcgcggt gctgcgcgtc gacgcgacga agctgccgtt cgtgcgcatc ggcgattcgg cgcgggtgcg cgccggcgaa ccggtgatga cgatcggcgc ccccgacggc tcgggcaata cggtgacggc cggcatcgtc agcgcgaccg cgcatccgct gccggacggc agcgcgtttc cgttcttcca gaccgacatc gcggtgaatc ccgacaactc cggcggcccg gtgttcaacc gtgcgggcga ggtgatcggc atcgcggtgc aggtctacac cggcgccgac cgctacgcga gcacgacgtt cgcgatcccg atcgcgttcg ccatgaaggt gcgcgcgcaa gcgcaggcgc agatgcggac caacgcacag gcgcagcgcg cttcgtccgg caacgcgctg ggcgtcgacg tacaggatgt cggcaccggg ttggccgccg cgttcgggct gccgcgtccg gtcggcgcgc tcgtcaacgg cgtcgatccc ggctcgccgg ccgccgcggc cggcgtgaag cccggcgacg tgatcgtgca gttcggcgac aagccgatcg gccaggctgc cgagctgaac gacctggccg ccgcgttgcc gcctggcggg aaggtgccgc tgcggctgat ccgcaaccgc acgccgatgc ggctgacggt cacgggagcg gcgaccggtg acgcgatcga caacgcgact tacaacgcga cttacaacgc gccctacaac gcgcccgcga cgggcagcgc aggcatggtc ggtacggccg gcgccgccgg cgtggcgatg gccgccgcgc ggccggccga tggcggcgat cggctcgggc tgacgatgca tccgctaagc gatgccgaac ggcgttcgac cgggctcgcg gtcgggatga tggtggacgc ggtgcacggg ccggccgcgc gcgcgggcat ccggccgggc gatgtcgtgc tggaactcaa cgacacgctg ctcgaaacgc cggaagacgt gccggcgctc gaagcgagcg gcggcgtgat cgcggtgctc atccagcgcg agaacgcgcg caagttcgtg tcggtgcggg cgcggtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat