BuamA.10100.b

Protease Do

CENTER ID: BuamA.10100.b
ORGANISM: Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.10100.b.B2.GE39797 Mature protein( BuamA.10100.b ) 30 494
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.10100.b.B2.PS37916 Mature protein( BuamA.10100.b ) 30 494

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|398577.6.peg.2609
RefSeq: YP_001809229.1
UniProt: B1YVV2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNTRFLARGA VAVAVAAALS AGYVAGTRHA DPQIITPAQA AALMPAEAAA KTGIPDFSGL VETYGPAVVN ISAKHVVKQV SRRVQQPQLP MDPSDPFYQF FKHFYGQVPG MGGDAQPDDQ PSASLGSGFI VSADGYILTN AHVIDGANVV TVKLTDKREY KAKVVGSDKQ SDVAVLKIDA SGLPTVKIGD PGQSKVGQWV VAIGSPYGFD NTVTSGIISA KSRALPDENY TPFIQTDVPV NPGNSGGPLF NLQGEVIGIN SMIYSQTGGF QGLSFAIPIN EAIKVKDELV KTGHVSRGRL GVAVQGLNQT LASSFGLQKP DGALVSSVDP NGPAAKAGLQ PGDVILGVNG SPVADSTSLP AQIANLKPGS KADLQVWRDK SKKTISVTLG AMTDAKLASN DGGPVEQGRL GVAVRPLTPQ ERSATNLSHG LIVQQAGGPA ATAGIQPGDV ILAVNGRPVT NAEQLRDAVK GAGNSLALLI QRDNAQIFVP VDLS
NT Sequence
ATGAACACTC GATTCCTTGC GCGCGGCGCC GTCGCGGTCG CCGTGGCGGC CGCCCTGTCC GCCGGCTATG TCGCGGGCAC CCGCCACGCC GATCCGCAGA TCATCACGCC TGCGCAGGCC GCTGCGCTGA TGCCGGCCGA GGCCGCGGCG AAGACGGGCA TTCCCGATTT CTCCGGGCTC GTCGAAACCT ACGGCCCGGC GGTCGTGAAC ATCAGCGCGA AGCACGTCGT GAAGCAGGTG TCGCGACGCG TGCAGCAGCC GCAACTGCCG ATGGATCCGA GCGATCCGTT CTACCAGTTC TTCAAGCACT TCTACGGCCA GGTGCCGGGC ATGGGCGGCG ACGCGCAGCC GGACGACCAG CCGAGCGCGA GCCTCGGTTC GGGCTTCATC GTCAGCGCCG ACGGGTACAT CCTCACGAAC GCGCACGTGA TCGACGGCGC GAACGTCGTC ACGGTCAAGC TGACCGACAA GCGCGAGTAC AAGGCGAAGG TCGTCGGCTC CGACAAGCAG TCCGATGTCG CGGTGCTGAA GATCGACGCG AGCGGCCTGC CGACCGTGAA GATCGGAGAC CCGGGCCAGA GCAAGGTCGG CCAGTGGGTC GTCGCGATCG GCTCGCCGTA CGGTTTCGAC AACACGGTCA CGTCCGGCAT CATCAGCGCG AAATCGCGCG CGCTGCCCGA CGAGAACTAC ACGCCGTTCA TCCAGACCGA CGTGCCGGTG AACCCCGGCA ACTCGGGCGG CCCGCTGTTC AACCTGCAGG GTGAGGTGAT CGGCATCAAC TCGATGATCT ACTCGCAGAC GGGCGGCTTC CAGGGCCTGT CGTTCGCGAT CCCGATCAAT GAGGCGATCA AGGTCAAGGA CGAGCTCGTG AAGACGGGCC ACGTGAGCCG CGGCCGCCTC GGCGTCGCGG TGCAGGGGCT GAACCAGACG CTCGCGAGCT CGTTCGGACT GCAAAAGCCG GATGGCGCGC TCGTCAGCTC GGTCGACCCG AACGGCCCGG CCGCGAAGGC CGGCCTGCAG CCGGGCGACG TGATCCTGGG GGTGAACGGC TCGCCGGTGG CCGACTCCAC GTCGCTGCCC GCGCAGATCG CGAACCTGAA GCCGGGCTCG AAGGCCGATC TGCAGGTGTG GCGCGACAAG TCGAAGAAGA CGATCAGCGT GACGCTCGGC GCGATGACCG ACGCGAAGCT GGCGTCGAAC GACGGCGGCC CGGTCGAGCA GGGGCGCCTC GGCGTCGCGG TGCGGCCGCT CACGCCGCAG GAGCGCAGCG CCACCAACCT GTCGCACGGG CTGATCGTGC AGCAGGCCGG CGGCCCGGCC GCGACCGCGG GCATCCAGCC CGGCGACGTG ATCCTCGCGG TCAACGGCCG CCCGGTCACG AACGCCGAGC AATTGCGCGA CGCGGTCAAG GGAGCGGGCA ACAGTCTTGC TCTCCTGATT CAGCGCGATA ATGCACAGAT CTTCGTGCCG GTCGACCTGA GC
Details for BuamA.10100.b.B2.GE39797
HARVESTED ON: 2/4/2016
SEQUENCED ON: 1/27/2016
EXPECTED MW: 49kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHAD PQIITPAQAA ALMPAEAAAK TGIPDFSGLV ETYGPAVVNI SAKHVVKQVS RRVQQPQLPM DPSDPFYQFF KHFYGQVPGM GGDAQPDDQP SASLGSGFIV SADGYILTNA HVIDGANVVT VKLTDKREYK AKVVGSDKQS DVAVLKIDAS GLPTVKIGDP GQSKVGQWVV AIGSPYGFDN TVTSGIISAK SRALPDENYT PFIQTDVPVN PGNSGGPLFN LQGEVIGINS MIYSQTGGFQ GLSFAIPINE AIKVKDELVK TGHVSRGRLG VAVQGLNQTL ASSFGLQKPD GALVSSVDPN GPAAKAGLQP GDVILGVNGS PVADSTSLPA QIANLKPGSK ADLQVWRDKS KKTISVTLGA MTDAKLASND GGPVEQGRLG VAVRPLTPQE RSATNLSHGL IVQQAGGPAA TAGIQPGDVI LAVNGRPVTN AEQLRDAVKG AGNSLALLIQ RDNAQIFVPV DLS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgccgatc cgcagatcat cacgcctgcg caggccgctg cgctgatgcc ggccgaggcc gcggcgaaga cgggcattcc cgatttctcc gggctcgtcg aaacctacgg cccggcggtc gtgaacatca gcgcgaagca cgtcgtgaag caggtgtcgc gacgcgtgca gcagccgcaa ctgccgatgg atccgagcga tccgttctac cagttcttca agcacttcta cggccaggtg ccgggcatgg gcggcgacgc gcagccggac gaccagccga gcgcgagcct cggttcgggc ttcatcgtca gcgccgacgg gtacatcctc acgaacgcgc acgtgatcga cggcgcgaac gtcgtcacgg tcaagctgac cgacaagcgc gagtacaagg cgaaggtcgt cggctccgac aagcagtccg atgtcgcggt gctgaagatc gacgcgagcg gcctgccgac cgtgaagatc ggagacccgg gccagagcaa ggtcggccag tgggtcgtcg cgatcggctc gccgtacggt ttcgacaaca cggtcacgtc cggcatcatc agcgcgaaat cgcgcgcgct gcccgacgag aactacacgc cgttcatcca gaccgacgtg ccggtgaacc ccggcaactc gggcggcccg ctgttcaacc tgcagggtga ggtgatcggc atcaactcga tgatctactc gcagacgggc ggcttccagg gcctgtcgtt cgcgatcccg atcaatgagg cgatcaaggt caaggacgag ctcgtgaaga cgggccacgt gagccgcggc cgcctcggcg tcgcggtgca ggggctgaac cagacgctcg cgagctcgtt cggactgcaa aagccggatg gcgcgctcgt cagctcggtc gacccgaacg gcccggccgc gaaggccggc ctgcagccgg gcgacgtgat cctgggggtg aacggctcgc cggtggccga ctccacgtcg ctgcccgcgc agatcgcgaa cctgaagccg ggctcgaagg ccgatctgca ggtgtggcgc gacaagtcga agaagacgat cagcgtgacg ctcggcgcga tgaccgacgc gaagctggcg tcgaacgacg gcggcccggt cgagcagggg cgcctcggcg tcgcggtgcg gccgctcacg ccgcaggagc gcagcgccac caacctgtcg cacgggctga tcgtgcagca ggccggcggc ccggccgcga ccgcgggcat ccagcccggc gacgtgatcc tcgcggtcaa cggccgcccg gtcacgaacg ccgagcaatt gcgcgacgcg gtcaagggag cgggcaacag tcttgctctc ctgattcagc gcgataatgc acagatcttc gtgccggtcg acctgagctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for BuamA.10100.b.B2.PS37916
PURIFICATION DATe: 4/19/2016
CONCENTRATION: 23.9mg/ml
OBSERVED MW: 49kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHAD PQIITPAQAA ALMPAEAAAK TGIPDFSGLV ETYGPAVVNI SAKHVVKQVS RRVQQPQLPM DPSDPFYQFF KHFYGQVPGM GGDAQPDDQP SASLGSGFIV SADGYILTNA HVIDGANVVT VKLTDKREYK AKVVGSDKQS DVAVLKIDAS GLPTVKIGDP GQSKVGQWVV AIGSPYGFDN TVTSGIISAK SRALPDENYT PFIQTDVPVN PGNSGGPLFN LQGEVIGINS MIYSQTGGFQ GLSFAIPINE AIKVKDELVK TGHVSRGRLG VAVQGLNQTL ASSFGLQKPD GALVSSVDPN GPAAKAGLQP GDVILGVNGS PVADSTSLPA QIANLKPGSK ADLQVWRDKS KKTISVTLGA MTDAKLASND GGPVEQGRLG VAVRPLTPQE RSATNLSHGL IVQQAGGPAA TAGIQPGDVI LAVNGRPVTN AEQLRDAVKG AGNSLALLIQ RDNAQIFVPV DLS
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGCCGATC CGCAGATCAT CACGCCTGCG CAGGCCGCTG CGCTGATGCC GGCCGAGGCC GCGGCGAAGA CGGGCATTCC CGATTTCTCC GGGCTCGTCG AAACCTACGG CCCGGCGGTC GTGAACATCA GCGCGAAGCA CGTCGTGAAG CAGGTGTCGC GACGCGTGCA GCAGCCGCAA CTGCCGATGG ATCCGAGCGA TCCGTTCTAC CAGTTCTTCA AGCACTTCTA CGGCCAGGTG CCGGGCATGG GCGGCGACGC GCAGCCGGAC GACCAGCCGA GCGCGAGCCT CGGTTCGGGC TTCATCGTCA GCGCCGACGG GTACATCCTC ACGAACGCGC ACGTGATCGA CGGCGCGAAC GTCGTCACGG TCAAGCTGAC CGACAAGCGC GAGTACAAGG CGAAGGTCGT CGGCTCCGAC AAGCAGTCCG ATGTCGCGGT GCTGAAGATC GACGCGAGCG GCCTGCCGAC CGTGAAGATC GGAGACCCGG GCCAGAGCAA GGTCGGCCAG TGGGTCGTCG CGATCGGCTC GCCGTACGGT TTCGACAACA CGGTCACGTC CGGCATCATC AGCGCGAAAT CGCGCGCGCT GCCCGACGAG AACTACACGC CGTTCATCCA GACCGACGTG CCGGTGAACC CCGGCAACTC GGGCGGCCCG CTGTTCAACC TGCAGGGTGA GGTGATCGGC ATCAACTCGA TGATCTACTC GCAGACGGGC GGCTTCCAGG GCCTGTCGTT CGCGATCCCG ATCAATGAGG CGATCAAGGT CAAGGACGAG CTCGTGAAGA CGGGCCACGT GAGCCGCGGC CGCCTCGGCG TCGCGGTGCA GGGGCTGAAC CAGACGCTCG CGAGCTCGTT CGGACTGCAA AAGCCGGATG GCGCGCTCGT CAGCTCGGTC GACCCGAACG GCCCGGCCGC GAAGGCCGGC CTGCAGCCGG GCGACGTGAT CCTGGGGGTG AACGGCTCGC CGGTGGCCGA CTCCACGTCG CTGCCCGCGC AGATCGCGAA CCTGAAGCCG GGCTCGAAGG CCGATCTGCA GGTGTGGCGC GACAAGTCGA AGAAGACGAT CAGCGTGACG CTCGGCGCGA TGACCGACGC GAAGCTGGCG TCGAACGACG GCGGCCCGGT CGAGCAGGGG CGCCTCGGCG TCGCGGTGCG GCCGCTCACG CCGCAGGAGC GCAGCGCCAC CAACCTGTCG CACGGGCTGA TCGTGCAGCA GGCCGGCGGC CCGGCCGCGA CCGCGGGCAT CCAGCCCGGC GACGTGATCC TCGCGGTCAA CGGCCGCCCG GTCACGAACG CCGAGCAATT GCGCGACGCG GTCAAGGGAG CGGGCAACAG TCTTGCTCTC CTGATTCAGC GCGATAATGC ACAGATCTTC GTGCCGGTCG ACCTGAGCTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT