BuamA.01673.b

RND efflux system, outer membrane lipoprotein, NodT family

CENTER ID: BuamA.01673.b
ORGANISM: Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.01673.b.A2.GE31761 Mature protein( BuamA.01673.b ) 29 498
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|398577.6.peg.1458
RefSeq: YP_001808131.1
UniProt: B1YP41

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKSSPLSVRA GSCRAAVAAA VAALALAGCA NYIGIKSDKQ IAPASQFDTA QSLPAQGGQW PALDWANQFG DPQLPKLIDE ALEGSPSIAQ AQARIAKASS YIESSRSNLM PKAEASYSWT RELYSSNALF PPPYGGQWYS ENNALASASW DLDLWGKNRE RLHTAVSQEK AAEADMQQAR ITLAASVART YNSLAQLYAL RDIAQREITN RQTVGKITDG RVSAGLDTNV ERQTARGNIA TTQASLSDLD GQITTVRYQL AALLGKGPDR GLQIAAPVMS PAGDVALPGN LPADLVSRRP DIVAARWQVE AAMHDVKEAK AEFFPDVNLA AGFGFDAFGW GKFLNFASRQ AQFGPAIHLP IFDAGALRAQ LKGRYADFDL SVANYNQTLI NALNDVATQV ASIRAVDRQM DDAQRALDAS TRAYDLAVIR YKAGLSPQLQ VLTADSNRLA SEQTVTNLKM RRRDMQLALI KALGGGFDAT GTRLAAPDAD KPTKQAAN
NT Sequence
ATGAAATCCT CCCCGTTGTC CGTGCGCGCC GGGTCGTGCC GCGCCGCCGT CGCCGCCGCG GTTGCCGCGC TGGCGCTGGC GGGCTGCGCA AACTACATCG GCATCAAGAG CGACAAGCAG ATCGCTCCCG CGTCGCAATT CGACACCGCC CAGAGCCTGC CGGCCCAGGG CGGCCAGTGG CCGGCGCTCG ACTGGGCCAA CCAGTTCGGC GATCCGCAAT TGCCGAAGCT GATCGACGAG GCGCTCGAGG GCAGCCCGTC GATCGCGCAG GCGCAGGCGC GCATCGCGAA AGCATCGTCG TACATCGAAT CGTCGCGCTC GAACCTGATG CCGAAGGCCG AAGCCAGCTA CTCGTGGACG CGCGAGCTGT ATTCGTCGAA CGCGCTGTTC CCGCCGCCGT ACGGCGGCCA GTGGTACAGC GAGAACAACG CGCTCGCGAG CGCGTCGTGG GATCTCGACC TGTGGGGCAA GAATCGCGAG CGCCTGCACA CGGCCGTGTC GCAGGAAAAG GCCGCCGAAG CCGACATGCA GCAGGCGCGC ATCACGCTCG CGGCGTCGGT CGCCCGCACC TACAACTCGC TCGCGCAACT GTATGCGCTG CGCGACATCG CGCAACGTGA GATCACCAAC CGCCAGACGG TCGGCAAGAT CACCGACGGC CGCGTGTCGG CCGGCCTCGA CACCAATGTC GAACGCCAGA CCGCGCGAGG CAATATCGCG ACGACCCAGG CGTCGCTGTC CGACCTCGAC GGCCAGATCA CGACCGTGCG CTACCAGCTC GCCGCGCTGC TCGGCAAGGG TCCGGACCGC GGGCTGCAGA TCGCCGCGCC GGTGATGAGC CCGGCCGGCG ACGTCGCGCT GCCGGGCAAC CTGCCGGCCG ACCTCGTGTC GCGCCGCCCC GACATCGTCG CCGCGCGCTG GCAGGTCGAG GCCGCGATGC ACGACGTGAA GGAAGCGAAG GCCGAGTTCT TCCCGGACGT GAACCTCGCG GCCGGCTTCG GCTTCGATGC GTTCGGGTGG GGCAAATTCC TGAATTTCGC GAGCCGCCAG GCGCAGTTCG GCCCGGCCAT CCACCTGCCG ATCTTCGATG CGGGCGCGTT GCGCGCGCAG CTCAAGGGCC GTTATGCGGA CTTCGACCTG TCGGTCGCGA ACTACAACCA GACGCTGATC AACGCGCTGA ACGACGTCGC GACGCAGGTC GCGTCGATCC GCGCGGTCGA TCGCCAGATG GACGACGCGC AGCGTGCGCT CGACGCGTCG ACGCGTGCGT ACGATCTCGC TGTGATCCGC TACAAGGCCG GCCTGTCGCC CCAGCTCCAG GTACTGACCG CGGACAGCAA CCGCCTCGCA TCGGAACAGA CGGTGACCAA CCTGAAGATG CGCCGGCGCG ACATGCAGCT CGCGCTGATC AAGGCGCTCG GCGGCGGGTT CGACGCGACC GGCACGCGGC TCGCCGCGCC CGACGCCGAC AAGCCGACGA AACAGGCCGC CAAC
Details for BuamA.01673.b.A2.GE31761
HARVESTED ON: 3/8/2011
SEQUENCED ON: 3/14/2011
EXPECTED MW: 53kDa
OBSERVED MW: 53kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 90
PERCENT COVERAGE: 64
Validated AA Sequence
MCANYIGIKS DKQIAPASQF DTAQSLPAQG GQWPALDWAN QFGDPQLPKL IDEALEGSPS IAQAQARIAK ASSYIESSRS NLMPKAEASY SWTRELYSSN ALFPPPYGGQ WYSENNALAS ASWDLDLWGK NRERLHTAVS QEKAAEADMQ QARITLAASV ARTYNSLAQL YALRDIAQRE ITNRQTVGKI TDGRVSAGLD TNVERQTARG NIATTQASLS DLDGXITXVR YQLAALLGKG XDRGLQIAAP VMSPXGDVXL PGXLPADXVS RRPXXVXARW QVXAAMHXVX EAXXXFSXXV NLXXXXRLRX VXXAXX
Validated NT Sequence
gcngnncagc catgtttnnn nnnctcnnnn nnncnnnnnn nannnnnnnn ngnnnncnnn gnnagctcag acccngggtc ctggttcgat gtgcgcaaac tacatcggca tcaagagcga caagcagatc gctcccgcgt cgcaattcga caccgcccag agcctgccgg cccagggcgg ccagtggccg gcgctcgact gggccaacca gttcggcgat ccgcaattgc cgaagctgat cgacgaggcg ctcgagggca gcccgtcgat cgcgcaggcg caggcgcgca tcgcgaaagc atcgtcgtac atcgaatcgt cgcgctcgaa cctgatgccg aaggccgaag ccagctactc gtggacgcgc gagctgtatt cgtcgaacgc gctgttcccg ccgccgtacg gcggccagtg gtacagcgag aacaacgcgc tcgcgagcgc gtcgtgggat ctcgacctgt ggggcaagaa tcgcgagcgc ctgcacacgg ccgtgtcgca ggaaaaggcc gccgaagccg acatgcagca ggcgcgcatc acgctcgcgg cgtcggtcgc ccgcacctac aactcgctcg cgcaactgta tgcgctgcgc gacatcgcgc aacgtgagat caccaaccgc cagacggtcg gcaagatcac cgacggccgc gtgtcggccg gcctcgacac caatgtcgaa cgccagaccg cgcgaggcaa tatcgcgacg acccaggcgt cgctgtccga cctcgacggc nagatcacga ncgtgcgcta ccagctcgcc gcgctgctcg gcaagggtnn ngaccgcggg ctgcagatcg ccgcgccggt gatgagcccg gncggcgacg tcncgctgcc gggcnanctg ccggcngacc nngtgtcgcg ccgccccnac ntcgtcncng cgcgctggca ggtcnnngcc gcnatgcacg angtgaanga agcnaangnc nagttttccc nggangtgaa cctcncgncc ngnntncggc ttcgangcgt nnggngggca aantnctgaa tttcgcgngc cgcnnnnnnn ngttngggnn ggncnncnnn ntnnnnnnat nnnnnannnn gggnnnnnnt nnnnngnnnn nnnnngngna nntnnnannn nngnnnnnnn nnncnaannn nnnnnnnnnn nnnnnnnnnn cnncnnnnna nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMCANYIGIK SDKQIAPASQ FDTAQSLPAQ GGQWPALDWA NQFGDPQLPK LIDEALEGSP SIAQAQARIA KASSYIESSR SNLMPKAEAS YSWTRELYSS NALFPPPYGG QWYSENNALA SASWDLDLWG KNRERLHTAV SQEKAAEADM QQARITLAAS VARTYNSLAQ LYALRDIAQR EITNRQTVGK ITDGRVSAGL DTNVERQTAR GNIATTQASL SDLDGQITTV RYQLAALLGK GPDRGLQIAA PVMSPAGDVA LPGNLPADLV SRRPDIVAAR WQVEAAMHDV KEAKAEFFPD VNLAAGFGFD AFGWGKFLNF ASRQAQFGPA IHLPIFDAGA LRAQLKGRYA DFDLSVANYN QTLINALNDV ATQVASIRAV DRQMDDAQRA LDASTRAYDL AVIRYKAGLS PQLQVLTADS NRLASEQTVT NLKMRRRDMQ LALIKALGGG FDATGTRLAA PDADKPTKQA AN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtgcgcaaac tacatcggca tcaagagcga caagcagatc gctcccgcgt cgcaattcga caccgcccag agcctgccgg cccagggcgg ccagtggccg gcgctcgact gggccaacca gttcggcgat ccgcaattgc cgaagctgat cgacgaggcg ctcgagggca gcccgtcgat cgcgcaggcg caggcgcgca tcgcgaaagc atcgtcgtac atcgaatcgt cgcgctcgaa cctgatgccg aaggccgaag ccagctactc gtggacgcgc gagctgtatt cgtcgaacgc gctgttcccg ccgccgtacg gcggccagtg gtacagcgag aacaacgcgc tcgcgagcgc gtcgtgggat ctcgacctgt ggggcaagaa tcgcgagcgc ctgcacacgg ccgtgtcgca ggaaaaggcc gccgaagccg acatgcagca ggcgcgcatc acgctcgcgg cgtcggtcgc ccgcacctac aactcgctcg cgcaactgta tgcgctgcgc gacatcgcgc aacgtgagat caccaaccgc cagacggtcg gcaagatcac cgacggccgc gtgtcggccg gcctcgacac caatgtcgaa cgccagaccg cgcgaggcaa tatcgcgacg acccaggcgt cgctgtccga cctcgacggc cagatcacga ccgtgcgcta ccagctcgcc gcgctgctcg gcaagggtcc ggaccgcggg ctgcagatcg ccgcgccggt gatgagcccg gccggcgacg tcgcgctgcc gggcaacctg ccggccgacc tcgtgtcgcg ccgccccgac atcgtcgccg cgcgctggca ggtcgaggcc gcgatgcacg acgtgaagga agcgaaggcc gagttcttcc cggacgtgaa cctcgcggcc ggcttcggct tcgatgcgtt cgggtggggc aaattcctga atttcgcgag ccgccaggcg cagttcggcc cggccatcca cctgccgatc ttcgatgcgg gcgcgttgcg cgcgcagctc aagggccgtt atgcggactt cgacctgtcg gtcgcgaact acaaccagac gctgatcaac gcgctgaacg acgtcgcgac gcaggtcgcg tcgatccgcg cggtcgatcg ccagatggac gacgcgcagc gtgcgctcga cgcgtcgacg cgtgcgtacg atctcgctgt gatccgctac aaggccggcc tgtcgcccca gctccaggta ctgaccgcgg acagcaaccg cctcgcatcg gaacagacgg tgaccaacct gaagatgcgc cggcgcgaca tgcagctcgc gctgatcaag gcgctcggcg gcgggttcga cgcgaccggc acgcggctcg ccgcgcccga cgccgacaag ccgacgaaac aggccgccaa ctaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa