BuamA.01666.c

Signal transduction histidine kinase, nitrogen specific, NtrB

CENTER ID: BuamA.01666.c
ORGANISM: Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.01666.c.A1.GE31755 Full length( BuamA.01666.c ) 1 380
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
OrthoMCL: OG5_145042
PATRIC ID: fig|398577.6.peg.2112
RefSeq: YP_001808755.1
UniProt: B1YT50

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MALKNLIKAK TGQPERLTED ERLARSGLLA GLEALPTVVI VLDRKTLRVA FANPSAEAML DISRRQLAQR PWGEIFPNAN ELAATITAIG QERFHATHLD TVLDRPGHEA LHVHAIVGFL ETAPDFVLVE LFENERQSRT DREERIHDLT AVNKQLIRNL AHEIKNPLGG IRGAAQLLEF ELSERERGEL REYTQVIIKE SDRLQTLVDR LLEPHRHPHI VGDVNIHEVC ERVRAVMLAE FPRGLTIERD YDVSVPDLRG DKEQLIQALL NIVRNAAQAL RERIAQGDAK IELRTRVARK VTVAKRLYKL ALDLHVVDNG PGIPEEIRDR IFYPLVSGRD DGSGLGLTLA QTFVQQHDGM IEVESRPGRT EFQILLPLDH
NT Sequence
ATGGCTCTGA AAAATCTGAT CAAGGCGAAG ACGGGGCAGC CCGAGCGACT GACGGAAGAC GAGCGGCTCG CGCGCTCGGG CCTGCTTGCG GGGCTGGAGG CACTGCCGAC GGTCGTGATC GTGCTCGACC GCAAGACGCT CAGGGTCGCG TTCGCGAACC CGTCCGCGGA GGCGATGCTC GACATCTCGC GGCGGCAGCT CGCGCAGCGG CCGTGGGGCG AGATTTTTCC GAACGCGAAC GAGCTCGCGG CGACGATCAC CGCGATCGGC CAGGAGCGTT TTCACGCGAC GCATCTCGAC ACGGTGCTCG ACCGGCCCGG CCACGAGGCG CTGCACGTGC ATGCGATCGT CGGCTTCCTC GAGACCGCGC CCGATTTCGT GCTCGTCGAA CTGTTCGAGA ACGAACGGCA GTCGCGCACC GATCGCGAGG AACGGATCCA CGACCTCACC GCGGTCAACA AGCAACTGAT CCGCAATCTC GCGCACGAGA TCAAGAACCC GCTCGGCGGC ATTCGCGGCG CAGCCCAGCT GCTCGAATTC GAGCTGAGCG AGCGCGAGCG CGGCGAGCTG CGCGAATACA CGCAGGTGAT CATCAAGGAG TCCGACCGCC TGCAGACGCT CGTCGACCGC TTGCTGGAGC CGCACCGGCA TCCGCATATC GTCGGCGACG TGAACATTCA TGAAGTGTGC GAACGCGTGC GCGCGGTGAT GCTCGCGGAA TTTCCGCGCG GCCTCACGAT CGAGCGCGAC TACGACGTCA GCGTGCCCGA CCTGCGCGGC GACAAGGAGC AGTTGATCCA GGCGCTGCTG AACATCGTGC GCAACGCCGC GCAGGCGCTG CGCGAACGGA TCGCGCAGGG CGACGCGAAG ATCGAACTGC GCACGCGCGT CGCGCGCAAG GTGACGGTCG CGAAGCGACT GTACAAGTTG GCACTGGACT TGCATGTAGT CGACAACGGG CCCGGCATTC CGGAAGAGAT CCGCGACCGG ATCTTCTATC CGCTCGTGTC CGGGCGCGAC GACGGCAGCG GCCTTGGCCT CACGCTCGCG CAGACCTTCG TGCAGCAGCA CGACGGGATG ATCGAGGTCG AGAGCCGGCC GGGACGTACC GAATTTCAGA TCCTGCTGCC GCTCGACCAT
Details for BuamA.01666.c.A1.GE31755
HARVESTED ON: 3/8/2011
SEQUENCED ON: 3/14/2011
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MALKNLIKAK TGQPERLTED ERLARSGLLA GLEALPTVVI VLDRKTLRVA FANPSAEAML DISRRQLAQR PWGEIFPNAN ELAATITAIG QERFHATHLD TVLDRPGHEA LHVHAIVGFL ETAPDFVLVE LFENERQSRT DREERIHDLT AVNKQLIRNL AHEIKNPLGG IRGAAQLLEF ELSERERGEL REYTQVIIKE SDRLQTLVDR LLEPHRHPHI VGDVNIHEVC ERVRAVMLAE FPRGLTIERD YDVSVPDLRG DKEQLIQALL NIVRNAAQAL RERIAQGDAK IELRTRVARK VTVAKRLYKL ALDLHVVDNG PGIPEEIRDR IFYPLVSGRD DGSGLGLTLA QTFVQQHDGM IEVESRPGRT EFQILLPL
Validated NT Sequence
cgagcggcag caggatctga aattcggtac gtcccggccg gctctcgacc tcgatcatcc cgtcgtgctg ctgcacgaag gtctgcgcga gcgtgaggcc aaggccgctg ccgtcgtcgc gcccggacac gagcggatag aagatccggt cgcggatctc ttccggaatg ccgggcccgt tgtcgactac atgcaagtcc agtgccaact tgtacagtcg cttcgcgacc gtcaccttgc gcgcgacgcg cgtgcgcagt tcgatcttcg cgtcgccctg cgcgatccgt tcgcgcagcg cctgcgcggc gttgcgcacg atgttcagca gcgcctggat caactgctcc ttgtcgccgc gcaggtcggg cacgctgacg tcgtagtcgc gctcgatcgt gaggccgcgc ggaaattccg cgagcatcac cgcgcgcacg cgttcgcaca cttcatgaat gttcacgtcg ccgacgatat gcggatgccg gtgcggctcc agcaagcggt cgacgagcgt ctgcaggcgg tcggactcct tgatgatcac ctgcgtgtat tcgcgcagct cgccgcgctc gcgctcgctc agctcgaatt cgagcagctg ggctgcgccg cgaatgccgc cgagcgggtt cttgatctcg tgcgcgagat tgcggatcag ttgcttgttg accgcggtga ggtcgtggat ccgttcctcg cgatcggtgc gcgactgccg ttcgttctcg aacagttcga cgagcacgaa atcgggcgcg gtctcgagga agccgacgat cgcatgcacg tgcagcgcct cgtggccggg ccggtcgagc accgtgtcga gatgcgtcgc gtgaaaacgc tcctggccga tcgcggtgat cgtcgccgcg agctcgttcg cgttcggaaa aatctcgccc cacggccgct gcgcgagctg ccgccgcgag atgtcgagca tcgcctccgc ggacgggttc gcgaacgcga ccctgagcgt cttgcggtcg agcacgatca cgaccgtcgg cagtgcctcc agccccgcaa gcaggcccga gcgcgcgagc cgctcgtctt ccgtcagtcg ctcgggctgc cccgtcttcg ccttgatcag atttttcaga gccatcgaac caggaccctg ggtctgagct tccagg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMALKNLIKA KTGQPERLTE DERLARSGLL AGLEALPTVV IVLDRKTLRV AFANPSAEAM LDISRRQLAQ RPWGEIFPNA NELAATITAI GQERFHATHL DTVLDRPGHE ALHVHAIVGF LETAPDFVLV ELFENERQSR TDREERIHDL TAVNKQLIRN LAHEIKNPLG GIRGAAQLLE FELSERERGE LREYTQVIIK ESDRLQTLVD RLLEPHRHPH IVGDVNIHEV CERVRAVMLA EFPRGLTIER DYDVSVPDLR GDKEQLIQAL LNIVRNAAQA LRERIAQGDA KIELRTRVAR KVTVAKRLYK LALDLHVVDN GPGIPEEIRD RIFYPLVSGR DDGSGLGLTL AQTFVQQHDG MIEVESRPGR TEFQILLPLD H
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggctctgaaa aatctgatca aggcgaagac ggggcagccc gagcgactga cggaagacga gcggctcgcg cgctcgggcc tgcttgcggg gctggaggca ctgccgacgg tcgtgatcgt gctcgaccgc aagacgctca gggtcgcgtt cgcgaacccg tccgcggagg cgatgctcga catctcgcgg cggcagctcg cgcagcggcc gtggggcgag atttttccga acgcgaacga gctcgcggcg acgatcaccg cgatcggcca ggagcgtttt cacgcgacgc atctcgacac ggtgctcgac cggcccggcc acgaggcgct gcacgtgcat gcgatcgtcg gcttcctcga gaccgcgccc gatttcgtgc tcgtcgaact gttcgagaac gaacggcagt cgcgcaccga tcgcgaggaa cggatccacg acctcaccgc ggtcaacaag caactgatcc gcaatctcgc gcacgagatc aagaacccgc tcggcggcat tcgcggcgca gcccagctgc tcgaattcga gctgagcgag cgcgagcgcg gcgagctgcg cgaatacacg caggtgatca tcaaggagtc cgaccgcctg cagacgctcg tcgaccgctt gctggagccg caccggcatc cgcatatcgt cggcgacgtg aacattcatg aagtgtgcga acgcgtgcgc gcggtgatgc tcgcggaatt tccgcgcggc ctcacgatcg agcgcgacta cgacgtcagc gtgcccgacc tgcgcggcga caaggagcag ttgatccagg cgctgctgaa catcgtgcgc aacgccgcgc aggcgctgcg cgaacggatc gcgcagggcg acgcgaagat cgaactgcgc acgcgcgtcg cgcgcaaggt gacggtcgcg aagcgactgt acaagttggc actggacttg catgtagtcg acaacgggcc cggcattccg gaagagatcc gcgaccggat cttctatccg ctcgtgtccg ggcgcgacga cggcagcggc cttggcctca cgctcgcgca gaccttcgtg cagcagcacg acgggatgat cgaggtcgag agccggccgg gacgtaccga atttcagatc ctgctgccgc tcgaccatta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a