SSGCID: Target Page

BuamA.01666.a

Two component transcriptional regulator, Fis family

CENTER ID:	BuamA.01666.a
ORGANISM:	Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuamA.01666.a.A1.GE31753	Full length( BuamA.01666.a )		1	233

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|398577.6.peg.3167
RefSeq:	YP_001809750.1
UniProt:	B1YPX0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MATILVVDDE MGIRELLSEI LSDEGHVVEA AENAQAAREY RLNQAPDLVL LDIWMPDTDG VTLLKEWAAQ GLLTMPVIMM SGHATIDTAV EATKIGALDF LEKPIALQKL LKSVEHGLVR GAAPVSVNSA AKAGVGQASG PAAVASAAAL PTLGDDMAAA LGLAGQTAAI PFDIPLREAR DAFERAYFEY HLARENGSMT RVAEKTGLER THLYRKLKQL GVELGKKPSE GAA

NT Sequence

ATGGCAACCA TCCTGGTGGT AGATGATGAA ATGGGCATCC GGGAATTGCT CTCGGAAATC CTCAGCGATG AAGGACATGT CGTCGAGGCG GCGGAGAACG CGCAGGCCGC GCGGGAATAC CGTCTGAATC AGGCGCCCGA TCTCGTGCTG CTCGATATCT GGATGCCCGA TACCGACGGC GTCACGCTGC TCAAGGAATG GGCGGCGCAG GGCCTGTTGA CGATGCCCGT GATCATGATG TCCGGGCACG CGACGATCGA CACGGCCGTC GAGGCAACGA AGATCGGCGC GCTCGATTTC CTCGAGAAGC CGATCGCGCT GCAGAAGCTG CTGAAGTCGG TCGAGCACGG CCTCGTGCGC GGCGCGGCGC CGGTGTCCGT CAACTCGGCG GCGAAGGCCG GCGTCGGCCA GGCTTCGGGG CCGGCGGCGG TCGCGTCCGC GGCCGCGCTG CCGACGCTCG GCGACGACAT GGCCGCGGCC CTGGGCCTCG CGGGGCAGAC GGCCGCGATT CCGTTCGACA TCCCGTTGCG CGAAGCGCGC GATGCGTTCG AGCGCGCGTA CTTCGAATAT CACCTCGCGC GCGAGAACGG CAGCATGACG CGCGTTGCGG AGAAGACGGG TCTCGAGCGC ACGCACCTGT ATCGCAAGCT CAAGCAGCTG GGTGTCGAGC TCGGCAAGAA GCCGTCGGAA GGCGCCGCG

Details for BuamA.01666.a.A1.GE31753

HARVESTED ON:	3/8/2011
SEQUENCED ON:	3/14/2011
EXPECTED MW:	26kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	96

Validated AA Sequence

MGTLEAQTQG PGSMATILVV DDEMGIRELL SEILSDEGHV VEAAENAQAA REYRLNQAPD LVLLDIWMPD TDGVTLLKEW AAQGLLTMPV IMMSGHATID TAVEATKIGA LDFLEKPIAL QKLLKSVEHG LVRGAAPVSV NSAAKAGVGQ ASGPAAVASA AALPTLGDDM AAALGLAGQT AAIPFDIPLR EARDAFERAY FEYHLARENG SMTRVAEKTG LERTHLYRKL KQLGVELGKK PSEGAA

Validated NT Sequence

ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggcaacc atcctggtgg tagatgatga aatgggcatc cgggaattgc tctcggaaat cctcagcgat gaaggacatg tcgtcgaggc ggcggagaac gcgcaggccg cgcgggaata ccgtctgaat caggcgcccg atctcgtgct gctcgatatc tggatgcccg ataccgacgg cgtcacgctg ctcaaggaat gggcggcgca gggcctgttg acgatgcccg tgatcatgat gtccgggcac gcgacgatcg acacggccgt cgaggcaacg aagatcggcg cgctcgattt cctcgagaag ccgatcgcgc tgcagaagct gctgaagtcg gtcgagcacg gcctcgtgcg cggcgcggcg ccggtgtccg tcaactcggc ggcgaaggcc ggcgtcggcc aggcttcggg gccggcggcg gtcgcgtccg cggccgcgct gccgacgctc ggcgacgaca tggccgcggc cctgggcctc gcggggcaga cggccgcgat tccgttcgac atcccgttgc gcgaagcgcg cgatgcgttc gagcgcgcgt acttcgaata tcacctcgcg cgcgagaacg gcagcatgac gcgcgttgcg gagaagacgg gtctcgagcg cacgcacctg tatcgcaagc tcaagcagct gggtgtcgag ctcggcaaga agccgtcgga aggcgccgcg taataa

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMATILVVDD EMGIRELLSE ILSDEGHVVE AAENAQAARE YRLNQAPDLV LLDIWMPDTD GVTLLKEWAA QGLLTMPVIM MSGHATIDTA VEATKIGALD FLEKPIALQK LLKSVEHGLV RGAAPVSVNS AAKAGVGQAS GPAAVASAAA LPTLGDDMAA ALGLAGQTAA IPFDIPLREA RDAFERAYFE YHLARENGSM TRVAEKTGLE RTHLYRKLKQ LGVELGKKPS EGAA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcaaccatc ctggtggtag atgatgaaat gggcatccgg gaattgctct cggaaatcct cagcgatgaa ggacatgtcg tcgaggcggc ggagaacgcg caggccgcgc gggaataccg tctgaatcag gcgcccgatc tcgtgctgct cgatatctgg atgcccgata ccgacggcgt cacgctgctc aaggaatggg cggcgcaggg cctgttgacg atgcccgtga tcatgatgtc cgggcacgcg acgatcgaca cggccgtcga ggcaacgaag atcggcgcgc tcgatttcct cgagaagccg atcgcgctgc agaagctgct gaagtcggtc gagcacggcc tcgtgcgcgg cgcggcgccg gtgtccgtca actcggcggc gaaggccggc gtcggccagg cttcggggcc ggcggcggtc gcgtccgcgg ccgcgctgcc gacgctcggc gacgacatgg ccgcggccct gggcctcgcg gggcagacgg ccgcgattcc gttcgacatc ccgttgcgcg aagcgcgcga tgcgttcgag cgcgcgtact tcgaatatca cctcgcgcgc gagaacggca gcatgacgcg cgttgcggag aagacgggtc tcgagcgcac gcacctgtat cgcaagctca agcagctggg tgtcgagctc ggcaagaagc cgtcggaagg cgccgcgtaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa