BuamA.00863.e
Histidine kinase
| CENTER ID: | BuamA.00863.e |
| ORGANISM: | Burkholderia ambifaria MC40-6 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuamA.00863.e.A1.GE31746 | Full length( BuamA.00863.e ) | 1 | 259 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuamA.00863.e.A1.PS01103 | Full length( BuamA.00863.e ) | 1 | 259 |
Structures
7LTQ
DEPOSITED: 2/19/2021
DETERMINATION: XRay
CLONE: BuamA.00863.e.A1.GE31746
PROTEIN: BuamA.00863.e.A1.PS01103
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_215291 |
| PATRIC ID: | fig|398577.6.peg.3868 |
| RefSeq: | YP_001810432.1 |
| UniProt: | B1Z136 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTSIPVDPAA DLLRERAAHY AAEAALFLRD QALSTASHDL RSPLNAMHSW AYVLERQLAS ADPSLQRALA GIRTGIDQQV ALIDDVLDAP RAETRTLAIT AQPFALRPLL DDTLALVRFA LADARQVSID ATLPDGEPSL SADRERVAQA LWTMLTTAVE ASAAGNRVTF ACTRDGAQCV AHVTCGVSAA ALADPALPHA FDAFARREML RSRDAKRVAW VLALCQRVAL AHGGTFTHAA FADGAVVTLS LAVPCKAAG
NT Sequence
GTGACGTCCA TTCCCGTCGA CCCTGCCGCC GACCTGCTGC GCGAACGCGC AGCGCACTAT GCCGCCGAGG CCGCGCTGTT CCTGCGCGAC CAGGCGCTTT CCACCGCGTC GCACGACCTG CGCAGCCCGC TGAATGCAAT GCACAGCTGG GCGTATGTGC TCGAGCGCCA GCTCGCGAGC GCCGACCCGA GCCTGCAGCG CGCGCTCGCG GGCATCCGCA CCGGGATCGA TCAGCAGGTT GCGCTGATCG ACGACGTGCT CGACGCGCCG CGCGCGGAAA CGCGCACCCT CGCGATCACC GCGCAGCCGT TCGCGCTGCG CCCGCTGCTC GACGACACGC TCGCGCTGGT GCGCTTCGCG CTCGCCGACG CGCGGCAGGT GAGCATCGAT GCGACGCTGC CCGACGGCGA GCCGTCACTG AGCGCCGATC GCGAACGCGT CGCGCAGGCG CTCTGGACGA TGCTGACGAC GGCCGTCGAA GCCAGTGCCG CCGGCAATCG CGTAACGTTC GCATGCACGC GCGACGGCGC GCAGTGCGTC GCGCATGTCA CCTGCGGCGT GAGCGCCGCC GCGCTCGCCG ATCCGGCGCT GCCGCACGCG TTCGACGCGT TCGCACGTCG CGAGATGCTG CGCTCGCGCG ACGCGAAGCG CGTCGCGTGG GTGCTCGCGC TGTGCCAGCG TGTCGCGCTC GCGCACGGCG GCACCTTCAC GCACGCCGCG TTCGCCGACG GCGCCGTCGT CACGCTGTCA CTCGCGGTTC CCTGCAAGGC GGCGGGC
Details for BuamA.00863.e.A1.GE31746
| HARVESTED ON: | 3/8/2011 |
| SEQUENCED ON: | 3/14/2011 |
| EXPECTED MW: | 29kDa |
| OBSERVED MW: | 29kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MGTLEAQTQG PGSMVTSIPV DPAADLLRER AAHYAAEAAL FLRDQALSTA SHDLRSPLNA MHSWAYVLER QLASADPSLQ RALAGIRTGI DQQVALIDDV LDAPRAETRT LAITAQPFAL RPLLDDTLAL VRFALADARQ VSIDATLPDG EPSLSADRER VAQALWTMLT TAVEASAAGN RVTFACTRDG AQCVAHVTCG VSAAALADPA LPHAFDAFAR REMLRSRDAK RVAWVLALCQ RVALAHGGTF THAAFADGAV VTLSLAVPCK AAG
Validated NT Sequence
ttattagccc gccgccttgc agggaaccgc gagtgacagc gtgacgacgg cgccgtcggc gaacgcggcg tgcgtgaagg tgccgccgtg cgcgagcgcg acacgctggc acagcgcgag cacccacgcg acgcgcttcg cgtcgcgcga gcgcagcatc tcgcgacgtg cgaacgcgtc gaacgcgtgc ggcagcgccg gatcggcgag cgcggcggcg ctcacgccgc aggtgacatg cgcgacgcac tgcgcgccgt cgcgcgtgca tgcgaacgtt acgcgattgc cggcggcact ggcttcgacg gccgtcgtca gcatcgtcca gagcgcctgc gcgacgcgtt cgcgatcggc gctcagtgac ggctcgccgt cgggcagcgt cgcatcgatg ctcacctgcc gcgcgtcggc gagcgcgaag cgcaccagcg cgagcgtgtc gtcgagcagc gggcgcagcg cgaacggctg cgcggtgatc gcgagggtgc gcgtttccgc gcgcggcgcg tcgagcacgt cgtcgatcag cgcaacctgc tgatcgatcc cggtgcggat gcccgcgagc gcgcgctgca ggctcgggtc ggcgctcgcg agctggcgct cgagcacata cgcccagctg tgcattgcat tcagcgggct gcgcaggtcg tgcgacgcgg tggaaagcgc ctggtcgcgc aggaacagcg cggcctcggc ggcatagtgc gctgcgcgtt cgcgcagcag gtcggcggca gggtcgacgg gaatggacgt caccatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagcca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTSIPVDP AADLLRERAA HYAAEAALFL RDQALSTASH DLRSPLNAMH SWAYVLERQL ASADPSLQRA LAGIRTGIDQ QVALIDDVLD APRAETRTLA ITAQPFALRP LLDDTLALVR FALADARQVS IDATLPDGEP SLSADRERVA QALWTMLTTA VEASAAGNRV TFACTRDGAQ CVAHVTCGVS AAALADPALP HAFDAFARRE MLRSRDAKRV AWVLALCQRV ALAHGGTFTH AAFADGAVVT LSLAVPCKAA G
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgacgtcc attcccgtcg accctgccgc cgacctgctg cgcgaacgcg cagcgcacta tgccgccgag gccgcgctgt tcctgcgcga ccaggcgctt tccaccgcgt cgcacgacct gcgcagcccg ctgaatgcaa tgcacagctg ggcgtatgtg ctcgagcgcc agctcgcgag cgccgacccg agcctgcagc gcgcgctcgc gggcatccgc accgggatcg atcagcaggt tgcgctgatc gacgacgtgc tcgacgcgcc gcgcgcggaa acgcgcaccc tcgcgatcac cgcgcagccg ttcgcgctgc gcccgctgct cgacgacacg ctcgcgctgg tgcgcttcgc gctcgccgac gcgcggcagg tgagcatcga tgcgacgctg cccgacggcg agccgtcact gagcgccgat cgcgaacgcg tcgcgcaggc gctctggacg atgctgacga cggccgtcga agccagtgcc gccggcaatc gcgtaacgtt cgcatgcacg cgcgacggcg cgcagtgcgt cgcgcatgtc acctgcggcg tgagcgccgc cgcgctcgcc gatccggcgc tgccgcacgc gttcgacgcg ttcgcacgtc gcgagatgct gcgctcgcgc gacgcgaagc gcgtcgcgtg ggtgctcgcg ctgtgccagc gtgtcgcgct cgcgcacggc ggcaccttca cgcacgccgc gttcgccgac ggcgccgtcg tcacgctgtc actcgcggtt ccctgcaagg cggcgggcta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for BuamA.00863.e.A1.PS01103
| PURIFICATION DATe: | 5/26/2011 |
| CONCENTRATION: | 28.3mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMVTSIPV DPAADLLRER AAHYAAEAAL FLRDQALSTA SHDLRSPLNA MHSWAYVLER QLASADPSLQ RALAGIRTGI DQQVALIDDV LDAPRAETRT LAITAQPFAL RPLLDDTLAL VRFALADARQ VSIDATLPDG EPSLSADRER VAQALWTMLT TAVEASAAGN RVTFACTRDG AQCVAHVTCG VSAAALADPA LPHAFDAFAR REMLRSRDAK RVAWVLALCQ RVALAHGGTF THAAFADGAV VTLSLAVPCK AAG
Validated NT Sequence
ttattagccc gccgccttgc agggaaccgc gagtgacagc gtgacgacgg cgccgtcggc gaacgcggcg tgcgtgaagg tgccgccgtg cgcgagcgcg acacgctggc acagcgcgag cacccacgcg acgcgcttcg cgtcgcgcga gcgcagcatc tcgcgacgtg cgaacgcgtc gaacgcgtgc ggcagcgccg gatcggcgag cgcggcggcg ctcacgccgc aggtgacatg cgcgacgcac tgcgcgccgt cgcgcgtgca tgcgaacgtt acgcgattgc cggcggcact ggcttcgacg gccgtcgtca gcatcgtcca gagcgcctgc gcgacgcgtt cgcgatcggc gctcagtgac ggctcgccgt cgggcagcgt cgcatcgatg ctcacctgcc gcgcgtcggc gagcgcgaag cgcaccagcg cgagcgtgtc gtcgagcagc gggcgcagcg cgaacggctg cgcggtgatc gcgagggtgc gcgtttccgc gcgcggcgcg tcgagcacgt cgtcgatcag cgcaacctgc tgatcgatcc cggtgcggat gcccgcgagc gcgcgctgca ggctcgggtc ggcgctcgcg agctggcgct cgagcacata cgcccagctg tgcattgcat tcagcgggct gcgcaggtcg tgcgacgcgg tggaaagcgc ctggtcgcgc aggaacagcg cggcctcggc ggcatagtgc gctgcgcgtt cgcgcagcag gtcggcggca gggtcgacgg gaatggacgt caccatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagcca
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTSIPVDP AADLLRERAA HYAAEAALFL RDQALSTASH DLRSPLNAMH SWAYVLERQL ASADPSLQRA LAGIRTGIDQ QVALIDDVLD APRAETRTLA ITAQPFALRP LLDDTLALVR FALADARQVS IDATLPDGEP SLSADRERVA QALWTMLTTA VEASAAGNRV TFACTRDGAQ CVAHVTCGVS AAALADPALP HAFDAFARRE MLRSRDAKRV AWVLALCQRV ALAHGGTFTH AAFADGAVVT LSLAVPCKAA G
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGACGTCC ATTCCCGTCG ACCCTGCCGC CGACCTGCTG CGCGAACGCG CAGCGCACTA TGCCGCCGAG GCCGCGCTGT TCCTGCGCGA CCAGGCGCTT TCCACCGCGT CGCACGACCT GCGCAGCCCG CTGAATGCAA TGCACAGCTG GGCGTATGTG CTCGAGCGCC AGCTCGCGAG CGCCGACCCG AGCCTGCAGC GCGCGCTCGC GGGCATCCGC ACCGGGATCG ATCAGCAGGT TGCGCTGATC GACGACGTGC TCGACGCGCC GCGCGCGGAA ACGCGCACCC TCGCGATCAC CGCGCAGCCG TTCGCGCTGC GCCCGCTGCT CGACGACACG CTCGCGCTGG TGCGCTTCGC GCTCGCCGAC GCGCGGCAGG TGAGCATCGA TGCGACGCTG CCCGACGGCG AGCCGTCACT GAGCGCCGAT CGCGAACGCG TCGCGCAGGC GCTCTGGACG ATGCTGACGA CGGCCGTCGA AGCCAGTGCC GCCGGCAATC GCGTAACGTT CGCATGCACG CGCGACGGCG CGCAGTGCGT CGCGCATGTC ACCTGCGGCG TGAGCGCCGC CGCGCTCGCC GATCCGGCGC TGCCGCACGC GTTCGACGCG TTCGCACGTC GCGAGATGCT GCGCTCGCGC GACGCGAAGC GCGTCGCGTG GGTGCTCGCG CTGTGCCAGC GTGTCGCGCT CGCGCACGGC GGCACCTTCA CGCACGCCGC GTTCGCCGAC GGCGCCGTCG TCACGCTGTC ACTCGCGGTT CCCTGCAAGG CGGCGGGCTA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A