BuamA.00863.c

Histidine kinase

CENTER ID: BuamA.00863.c
ORGANISM: Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.00863.c.A1.GE31744 Full length( BuamA.00863.c ) 1 371
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.00863.c.A1.PS01201 Full length( BuamA.00863.c ) 1 371

External Resources

RESOURCE REFERENCE ID
RefSeq: WP_012364195.1
RefSeq: YP_001808694.1
UniProt: B1YSP2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRLADFIVRN IEPILVQWEA FAATLLPAAR SMDSLALRDQ ARQILEAVAK DITTPQTRED QHEKSLGRAP QPVKATAAQT HAVLRARRGF NINQLAAEYR ALRASVLRLW IDECKPSAPD LDDMIRFNEA IDQALAESLA FFAEQVEQSR NLLLGMLGHD MRTPLQTIHV TASYLAALNA GEAVSDAAAR LIRSGSRMQG LLDDLCDYNR TQLGVGINVV PRNIDLARVL ANVVDELQVA HPDREINLEV SGDLRGDWDD QRMQRLLSNL VANAVKYGTP ETPVWVMAAS ADAEVLIEIR NSGPAMDRCT LDRIFDPLQR GAHQPDLPSD DVGLGLGLFI ASEIAKAHHG RIDARSDQTE TIFAVRLPKG A
NT Sequence
ATGCGTCTAG CTGATTTCAT TGTTAGGAAC ATAGAGCCGA TCCTGGTGCA GTGGGAAGCG TTTGCCGCCA CGCTGCTACC AGCCGCCAGG AGCATGGACT CGCTCGCGCT GCGCGACCAA GCACGCCAGA TCCTGGAGGC CGTAGCCAAG GACATAACGA CCCCGCAAAC AAGGGAAGAT CAACATGAAA AATCGTTGGG GCGCGCGCCC CAGCCGGTGA AGGCCACGGC CGCGCAGACG CATGCCGTTC TGCGCGCCCG GCGGGGTTTC AACATCAACC AGCTGGCGGC CGAGTATCGG GCGCTGCGTG CCAGTGTGCT GCGCTTATGG ATCGACGAGT GCAAGCCGAG CGCGCCTGAT CTGGACGACA TGATTCGCTT CAATGAAGCG ATAGATCAGG CGCTGGCGGA ATCGCTCGCT TTCTTCGCCG AACAGGTCGA GCAGTCTCGC AACTTGTTGC TCGGTATGCT GGGTCACGAC ATGCGGACGC CGTTGCAGAC CATTCACGTC ACAGCGTCCT ATCTTGCCGC GCTCAATGCG GGGGAGGCGG TATCAGACGC TGCCGCCCGG CTGATCAGAA GCGGCAGCCG CATGCAGGGC CTCCTTGACG ATCTCTGCGA TTACAACCGG ACCCAGCTCG GGGTAGGCAT CAACGTCGTT CCACGCAATA TCGATCTCGC CCGTGTGCTC GCCAATGTGG TCGATGAATT GCAGGTGGCT CATCCCGATC GCGAAATCAA TCTCGAGGTA AGCGGCGATC TCCGAGGGGA CTGGGACGAT CAGCGAATGC AGCGGTTGCT GAGCAATCTC GTGGCCAACG CCGTCAAGTA CGGAACGCCC GAGACACCGG TATGGGTGAT GGCAGCATCG GCTGATGCGG AAGTGCTCAT CGAGATCAGG AACAGTGGGC CGGCCATGGA TCGGTGCACG CTGGATCGCA TCTTCGACCC GCTTCAGCGC GGAGCGCATC AACCAGACTT GCCCAGTGAC GATGTTGGCT TGGGGCTTGG CTTGTTCATC GCCAGCGAAA TCGCAAAGGC ACACCACGGC CGTATTGACG CGCGGTCCGA TCAGACCGAA ACAATATTCG CGGTACGCCT GCCGAAAGGC GCA
Details for BuamA.00863.c.A1.GE31744
HARVESTED ON: 3/8/2011
SEQUENCED ON: 3/14/2011
EXPECTED MW: 43kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MRLADFIVRN IEPILVQWEA FAATLLPAAR SMDSLALRDQ ARQILEAVAK DITTPQTRED QHEKSLGRAP QPVKATAAQT HAVLRARRGF NINQLAAEYR ALRASVLRLW IDECKPSAPD LDDMIRFNEA IDQALAESLA FFAEQVEQSR NLLLGMLGHD MRTPLQTIHV TASYLAALNA GEAVSDAAAR LIRSGSRMQG LLDDLCDYNR TQLGVGINVV PRNIDLARVL ANVVDELQVA HPDREINLEV SGDLRGDWDD QRMQRLLSNL VANAVKYGTP ETPVWVMAAS ADAEVLIEIR NSGPAMDRCT LDRIFDPLQR GAHQPDLPSD DVGLGLGLFI ASEIAKAHHG RIDARSDQTE TIFAVRLPK
Validated NT Sequence
gcctttcggc aggcgtaccg cgaatattgt ttcggtctga tcggaccgcg cgtcaatacg gccgtggtgt gcctttgcga tttcgctggc gatgaacaag ccaagcccca agccaacatc gtcactgggc aagtctggtt gatgcgctcc gcgctgaagc gggtcgaaga tgcgatccag cgtgcaccga tccatggccg gcccactgtt cctgatctcg atgagcactt ccgcatcagc cgatgctgcc atcacccata ccggtgtctc gggcgttccg tacttgacgg cgttggccac gagattgctc agcaaccgct gcattcgctg atcgtcccag tcccctcgga gatcgccgct tacctcgaga ttgatttcgc gatcgggatg agccacctgc aattcatcga ccacattggc gagcacacgg gcgagatcga tattgcgtgg aacgacgttg atgcctaccc cgagctgggt ccggttgtaa tcgcagagat cgtcaaggag gccctgcatg cggctgccgc ttctgatcag ccgggcggca gcgtctgata ccgcctcccc cgcattgagc gcggcaagat aggacgctgt gacgtgaatg gtctgcaacg gcgtccgcat gtcgtgaccc agcataccga gcaacaagtt gcgagactgc tcgacctgtt cggcgaagaa agcgagcgat tccgccagcg cctgatctat cgcttcattg aagcgaatca tgtcgtccag atcaggcgcg ctcggcttgc actcgtcgat ccataagcgc agcacactgg cacgcagcgc ccgatactcg gccgccagct ggttgatgtt gaaaccccgc cgggcgcgca gaacggcatg cgtctgcgcg gccgtggcct tcaccggctg gggcgcgcgc cccaacgatt tttcatgttg atcttccctt gtttgcgggg tcgttatgtc cttggctacg gcctccagga tctggcgtgc ttggtcgcgc agcgcgagcg agtccatgct cctggcggct ggtagcagcg tggcggcaaa cgcttcccac tgcaccagga tcggctctat gttcctaaca atgaaatcag ctagacgcat cgaaccagga ccctgggtct gagct
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRLADFIVR NIEPILVQWE AFAATLLPAA RSMDSLALRD QARQILEAVA KDITTPQTRE DQHEKSLGRA PQPVKATAAQ THAVLRARRG FNINQLAAEY RALRASVLRL WIDECKPSAP DLDDMIRFNE AIDQALAESL AFFAEQVEQS RNLLLGMLGH DMRTPLQTIH VTASYLAALN AGEAVSDAAA RLIRSGSRMQ GLLDDLCDYN RTQLGVGINV VPRNIDLARV LANVVDELQV AHPDREINLE VSGDLRGDWD DQRMQRLLSN LVANAVKYGT PETPVWVMAA SADAEVLIEI RNSGPAMDRC TLDRIFDPLQ RGAHQPDLPS DDVGLGLGLF IASEIAKAHH GRIDARSDQT ETIFAVRLPK GA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgtctagct gatttcattg ttaggaacat agagccgatc ctggtgcagt gggaagcgtt tgccgccacg ctgctaccag ccgccaggag catggactcg ctcgcgctgc gcgaccaagc acgccagatc ctggaggccg tagccaagga cataacgacc ccgcaaacaa gggaagatca acatgaaaaa tcgttggggc gcgcgcccca gccggtgaag gccacggccg cgcagacgca tgccgttctg cgcgcccggc ggggtttcaa catcaaccag ctggcggccg agtatcgggc gctgcgtgcc agtgtgctgc gcttatggat cgacgagtgc aagccgagcg cgcctgatct ggacgacatg attcgcttca atgaagcgat agatcaggcg ctggcggaat cgctcgcttt cttcgccgaa caggtcgagc agtctcgcaa cttgttgctc ggtatgctgg gtcacgacat gcggacgccg ttgcagacca ttcacgtcac agcgtcctat cttgccgcgc tcaatgcggg ggaggcggta tcagacgctg ccgcccggct gatcagaagc ggcagccgca tgcagggcct ccttgacgat ctctgcgatt acaaccggac ccagctcggg gtaggcatca acgtcgttcc acgcaatatc gatctcgccc gtgtgctcgc caatgtggtc gatgaattgc aggtggctca tcccgatcgc gaaatcaatc tcgaggtaag cggcgatctc cgaggggact gggacgatca gcgaatgcag cggttgctga gcaatctcgt ggccaacgcc gtcaagtacg gaacgcccga gacaccggta tgggtgatgg cagcatcggc tgatgcggaa gtgctcatcg agatcaggaa cagtgggccg gccatggatc ggtgcacgct ggatcgcatc ttcgacccgc ttcagcgcgg agcgcatcaa ccagacttgc ccagtgacga tgttggcttg gggcttggct tgttcatcgc cagcgaaatc gcaaaggcac accacggccg tattgacgcg cggtccgatc agaccgaaac aatattcgcg gtacgcctgc cgaaaggcgc ataaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for BuamA.00863.c.A1.PS01201
PURIFICATION DATe: 9/8/2011
CONCENTRATION: 16.61mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 50µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Protocol Notes
notes unavailable
Validated AA Sequence
MRLADFIVRN IEPILVQWEA FAATLLPAAR SMDSLALRDQ ARQILEAVAK DITTPQTRED QHEKSLGRAP QPVKATAAQT HAVLRARRGF NINQLAAEYR ALRASVLRLW IDECKPSAPD LDDMIRFNEA IDQALAESLA FFAEQVEQSR NLLLGMLGHD MRTPLQTIHV TASYLAALNA GEAVSDAAAR LIRSGSRMQG LLDDLCDYNR TQLGVGINVV PRNIDLARVL ANVVDELQVA HPDREINLEV SGDLRGDWDD QRMQRLLSNL VANAVKYGTP ETPVWVMAAS ADAEVLIEIR NSGPAMDRCT LDRIFDPLQR GAHQPDLPSD DVGLGLGLFI ASEIAKAHHG RIDARSDQTE TIFAVRLPK
Validated NT Sequence
gcctttcggc aggcgtaccg cgaatattgt ttcggtctga tcggaccgcg cgtcaatacg gccgtggtgt gcctttgcga tttcgctggc gatgaacaag ccaagcccca agccaacatc gtcactgggc aagtctggtt gatgcgctcc gcgctgaagc gggtcgaaga tgcgatccag cgtgcaccga tccatggccg gcccactgtt cctgatctcg atgagcactt ccgcatcagc cgatgctgcc atcacccata ccggtgtctc gggcgttccg tacttgacgg cgttggccac gagattgctc agcaaccgct gcattcgctg atcgtcccag tcccctcgga gatcgccgct tacctcgaga ttgatttcgc gatcgggatg agccacctgc aattcatcga ccacattggc gagcacacgg gcgagatcga tattgcgtgg aacgacgttg atgcctaccc cgagctgggt ccggttgtaa tcgcagagat cgtcaaggag gccctgcatg cggctgccgc ttctgatcag ccgggcggca gcgtctgata ccgcctcccc cgcattgagc gcggcaagat aggacgctgt gacgtgaatg gtctgcaacg gcgtccgcat gtcgtgaccc agcataccga gcaacaagtt gcgagactgc tcgacctgtt cggcgaagaa agcgagcgat tccgccagcg cctgatctat cgcttcattg aagcgaatca tgtcgtccag atcaggcgcg ctcggcttgc actcgtcgat ccataagcgc agcacactgg cacgcagcgc ccgatactcg gccgccagct ggttgatgtt gaaaccccgc cgggcgcgca gaacggcatg cgtctgcgcg gccgtggcct tcaccggctg gggcgcgcgc cccaacgatt tttcatgttg atcttccctt gtttgcgggg tcgttatgtc cttggctacg gcctccagga tctggcgtgc ttggtcgcgc agcgcgagcg agtccatgct cctggcggct ggtagcagcg tggcggcaaa cgcttcccac tgcaccagga tcggctctat gttcctaaca atgaaatcag ctagacgcat cgaaccagga ccctgggtct gagct
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRLADFIVR NIEPILVQWE AFAATLLPAA RSMDSLALRD QARQILEAVA KDITTPQTRE DQHEKSLGRA PQPVKATAAQ THAVLRARRG FNINQLAAEY RALRASVLRL WIDECKPSAP DLDDMIRFNE AIDQALAESL AFFAEQVEQS RNLLLGMLGH DMRTPLQTIH VTASYLAALN AGEAVSDAAA RLIRSGSRMQ GLLDDLCDYN RTQLGVGINV VPRNIDLARV LANVVDELQV AHPDREINLE VSGDLRGDWD DQRMQRLLSN LVANAVKYGT PETPVWVMAA SADAEVLIEI RNSGPAMDRC TLDRIFDPLQ RGAHQPDLPS DDVGLGLGLF IASEIAKAHH GRIDARSDQT ETIFAVRLPK GA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCGTCTAGCT GATTTCATTG TTAGGAACAT AGAGCCGATC CTGGTGCAGT GGGAAGCGTT TGCCGCCACG CTGCTACCAG CCGCCAGGAG CATGGACTCG CTCGCGCTGC GCGACCAAGC ACGCCAGATC CTGGAGGCCG TAGCCAAGGA CATAACGACC CCGCAAACAA GGGAAGATCA ACATGAAAAA TCGTTGGGGC GCGCGCCCCA GCCGGTGAAG GCCACGGCCG CGCAGACGCA TGCCGTTCTG CGCGCCCGGC GGGGTTTCAA CATCAACCAG CTGGCGGCCG AGTATCGGGC GCTGCGTGCC AGTGTGCTGC GCTTATGGAT CGACGAGTGC AAGCCGAGCG CGCCTGATCT GGACGACATG ATTCGCTTCA ATGAAGCGAT AGATCAGGCG CTGGCGGAAT CGCTCGCTTT CTTCGCCGAA CAGGTCGAGC AGTCTCGCAA CTTGTTGCTC GGTATGCTGG GTCACGACAT GCGGACGCCG TTGCAGACCA TTCACGTCAC AGCGTCCTAT CTTGCCGCGC TCAATGCGGG GGAGGCGGTA TCAGACGCTG CCGCCCGGCT GATCAGAAGC GGCAGCCGCA TGCAGGGCCT CCTTGACGAT CTCTGCGATT ACAACCGGAC CCAGCTCGGG GTAGGCATCA ACGTCGTTCC ACGCAATATC GATCTCGCCC GTGTGCTCGC CAATGTGGTC GATGAATTGC AGGTGGCTCA TCCCGATCGC GAAATCAATC TCGAGGTAAG CGGCGATCTC CGAGGGGACT GGGACGATCA GCGAATGCAG CGGTTGCTGA GCAATCTCGT GGCCAACGCC GTCAAGTACG GAACGCCCGA GACACCGGTA TGGGTGATGG CAGCATCGGC TGATGCGGAA GTGCTCATCG AGATCAGGAA CAGTGGGCCG GCCATGGATC GGTGCACGCT GGATCGCATC TTCGACCCGC TTCAGCGCGG AGCGCATCAA CCAGACTTGC CCAGTGACGA TGTTGGCTTG GGGCTTGGCT TGTTCATCGC CAGCGAAATC GCAAAGGCAC ACCACGGCCG TATTGACGCG CGGTCCGATC AGACCGAAAC AATATTCGCG GTACGCCTGC CGAAAGGCGC ATAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA