BuamA.00171.b

Beta-ketoacyl-acyl-carrier-protein synthase I

CENTER ID: BuamA.00171.b
ORGANISM: Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.00171.b.A1.GE32125 Full length( BuamA.00171.b ) 1 368
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|398577.6.peg.5977
RefSeq: YP_001815521.1
UniProt: B1Z5C2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVHNVRIASL ACRLPARQVP NDDPVFAGVE PIPSEWWQFW GIESRYMIDP DAGESELALA ERTARDALAR AGVEAAELDL VLFNMTSPFV SLADGRRRFA PRLARALRDR LGATRALDAD VEMECASFVL QLQIATNLIK QGRVKRALVC SSERMSAVVD YTSKSSTNFG DGAAAAVLVA EPADPDGADW LDAAYHSDAT RYDLVTMQWR NARAAADDAA TDADNPFGVY FTLKPEAQEA ISRFMPVAIP DIVERLLHRS GTGIADIDAM VFHQPSALLV RAWAQRLGLK PDQYVIRVAD CACLVSAAVP FALYESIREG VIRPGSLVVI AGAGAGWGFG AQLWRWGETV VTDAGDTVAA VTEQEVRT
NT Sequence
ATGGTGCACA ACGTCCGAAT CGCAAGTCTG GCGTGCCGCC TGCCGGCACG CCAGGTACCC AACGACGATC CCGTCTTCGC GGGCGTCGAA CCCATTCCGT CGGAATGGTG GCAATTCTGG GGCATCGAAT CGCGCTACAT GATCGATCCC GATGCCGGCG AATCCGAGCT TGCGCTGGCC GAGCGCACGG CCCGCGACGC ACTCGCCCGT GCCGGCGTGG AGGCCGCCGA ACTCGATCTG GTGCTGTTCA ACATGACGTC GCCGTTCGTC TCGCTCGCCG ACGGCCGGCG CCGTTTCGCG CCGCGGCTGG CGCGTGCGCT GCGCGACAGG CTCGGCGCCA CGCGCGCGCT CGATGCCGAT GTCGAGATGG AATGCGCGAG TTTCGTGCTG CAACTGCAGA TCGCCACCAA CCTCATCAAG CAGGGACGGG TGAAGCGCGC CCTGGTGTGC TCGTCGGAGC GCATGTCGGC CGTGGTCGAC TACACCAGTA AGTCGTCGAC GAACTTCGGC GATGGCGCGG CCGCGGCCGT GCTCGTCGCC GAGCCGGCCG ATCCCGATGG CGCCGATTGG CTGGATGCCG CTTACCACAG CGATGCAACC CGCTACGACC TCGTGACGAT GCAGTGGCGC AATGCGCGGG CGGCGGCAGA CGACGCGGCA ACCGACGCCG ACAACCCGTT CGGCGTGTAT TTCACGCTCA AGCCCGAAGC GCAGGAGGCG ATCTCGCGTT TCATGCCGGT GGCCATTCCC GATATCGTCG AGCGGCTGTT GCACCGGAGC GGAACGGGCA TCGCCGACAT CGACGCGATG GTGTTCCATC AGCCGTCGGC GCTGCTCGTG CGCGCATGGG CGCAGCGGCT CGGGCTCAAG CCCGACCAAT ACGTGATTCG CGTGGCCGAT TGTGCGTGCC TCGTGTCGGC CGCCGTGCCG TTTGCGCTGT ACGAGTCGAT CCGGGAAGGC GTGATCCGGC CAGGATCGTT GGTCGTGATT GCCGGCGCAG GCGCCGGCTG GGGCTTCGGC GCGCAATTGT GGCGCTGGGG CGAAACCGTC GTGACCGATG CGGGCGATAC CGTCGCCGCC GTGACGGAGC AGGAGGTGCG GACA
Details for BuamA.00171.b.A1.GE32125
HARVESTED ON: 4/26/2011
SEQUENCED ON: 5/2/2011
EXPECTED MW: 42kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 63
PERCENT COVERAGE: 93
Validated AA Sequence
MFXXLXXXXX XXXXXXXXXX GPGSMVHNVR IASLACRLPA RQVPNDDPVF AGVEPIPSEW WQFWGIESRY MIDPDAGESE LALAERTARD ALARAGVEAA ELDLVLFNMT SPFVSLADGR RRFAPRLARA LRDRLGATRA LDADVEMECA SFVLQLQIAT NLIKQGRVKR ALVCSSERMS AVVDYTSKSS TNFGDGAAAA VLVAEPADPD GADWLDAAYH SDATPLRPRD DAVAQCAGGG RXXGNRRRQP XSACISRSSP EAQXAISRFH XGGPXPISSS GCCTGAXXGX XDXRRDXCSX XXRXLLXXAX XQRLXXXSPN QXXXXXXXRX XXXXXXARXX LXXXXXXXXX XXXXXXXXXX XX
Validated NT Sequence
gcggnncngc catgtttnnn tnnctannnn nnnnnnnnna cnannnnnnn nnnnnnnnnn tcannnccnn nggtcctggt tcgatggtgc acaacgtccg aatcgcaagt ctggcgtgcc gcctgccggc acgccaggta cccaacgacg atcccgtctt cgcgggcgtc gaacccattc cgtcggaatg gtggcaattc tggggcatcg aatcgcgcta catgatcgat cccgatgccg gcgaatccga gcttgcgctg gccgagcgca cggcccgcga cgcactcgcc cgtgccggcg tggaggccgc cgaactcgat ctggtgctgt tcaacatgac gtcgccgttc gtctcgctcg ccgacggccg gcgccgtttc gcgccgcggc tggcgcgtgc gctgcgcgac aggctcggcg ccacgcgcgc gctcgatgcc gatgtcgaga tggaatgcgc gagtttcgtg ctgcaactgc agatcgccac caacctcatc aagcagggac gggtgaagcg cgccctggtg tgctcgtcgg agcgcatgtc ggccgtggtc gactacacca gtaagtcgtc gacgaacttc ggcgatggcg cggccgcggc cgtgctcgtc gccgagccgg ccgatcccga tggcgccgat tggctggatg ccgcttacca cagcgatgca accccgctac gacctcgtga cgatgcagtg gcgcaatgcg cgggcggcgg cagacnannc ggcaaccgac gccgacaacc cngttcggcg tgtatttcac gctcaagccc cgaagcgcag gangcgatct cgcgttttca tgncggtggn ccantcccga tatcgtcgag cggctgttgc accggagcgg nacngggcat nnccgacnnt cgacgcgatg ngtgttcnnt canccntcgg gnnctgctcn ngnncgcann nnnncagcgg ctnngggntn nnagcccnaa ccaatnannn nnnttnnnnn nngnccgann nngcnnnncn tngtnncngg cccgtnnccn nttgnnnnnn nacganncna annnggnann ncntnnnncc nncnnggnnt nnntggnnnn nnnnnnncc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVHNVRIAS LACRLPARQV PNDDPVFAGV EPIPSEWWQF WGIESRYMID PDAGESELAL AERTARDALA RAGVEAAELD LVLFNMTSPF VSLADGRRRF APRLARALRD RLGATRALDA DVEMECASFV LQLQIATNLI KQGRVKRALV CSSERMSAVV DYTSKSSTNF GDGAAAAVLV AEPADPDGAD WLDAAYHSDA TRYDLVTMQW RNARAAADDA ATDADNPFGV YFTLKPEAQE AISRFMPVAI PDIVERLLHR SGTGIADIDA MVFHQPSALL VRAWAQRLGL KPDQYVIRVA DCACLVSAAV PFALYESIRE GVIRPGSLVV IAGAGAGWGF GAQLWRWGET VVTDAGDTVA AVTEQEVRT
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgcacaac gtccgaatcg caagtctggc gtgccgcctg ccggcacgcc aggtacccaa cgacgatccc gtcttcgcgg gcgtcgaacc cattccgtcg gaatggtggc aattctgggg catcgaatcg cgctacatga tcgatcccga tgccggcgaa tccgagcttg cgctggccga gcgcacggcc cgcgacgcac tcgcccgtgc cggcgtggag gccgccgaac tcgatctggt gctgttcaac atgacgtcgc cgttcgtctc gctcgccgac ggccggcgcc gtttcgcgcc gcggctggcg cgtgcgctgc gcgacaggct cggcgccacg cgcgcgctcg atgccgatgt cgagatggaa tgcgcgagtt tcgtgctgca actgcagatc gccaccaacc tcatcaagca gggacgggtg aagcgcgccc tggtgtgctc gtcggagcgc atgtcggccg tggtcgacta caccagtaag tcgtcgacga acttcggcga tggcgcggcc gcggccgtgc tcgtcgccga gccggccgat cccgatggcg ccgattggct ggatgccgct taccacagcg atgcaacccg ctacgacctc gtgacgatgc agtggcgcaa tgcgcgggcg gcggcagacg acgcggcaac cgacgccgac aacccgttcg gcgtgtattt cacgctcaag cccgaagcgc aggaggcgat ctcgcgtttc atgccggtgg ccattcccga tatcgtcgag cggctgttgc accggagcgg aacgggcatc gccgacatcg acgcgatggt gttccatcag ccgtcggcgc tgctcgtgcg cgcatgggcg cagcggctcg ggctcaagcc cgaccaatac gtgattcgcg tggccgattg tgcgtgcctc gtgtcggccg ccgtgccgtt tgcgctgtac gagtcgatcc gggaaggcgt gatccggcca ggatcgttgg tcgtgattgc cggcgcaggc gccggctggg gcttcggcgc gcaattgtgg cgctggggcg aaaccgtcgt gaccgatgcg ggcgataccg tcgccgccgt gacggagcag gaggtgcgga cataaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa