BuamA.00163.a

Phenylalanyl-tRNA synthetase, alpha subunit

CENTER ID: BuamA.00163.a
ORGANISM: Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.00163.a.B1.GE39142 Full length( BuamA.00163.a ) 1 345
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|398577.6.peg.1433
RefSeq: YP_001808107.1
UniProt: B1YP17

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTLEMMGSMD LDQIVADAQQ SFEQAADITT LENEKARFLG KSGALTELLK GLGKLDPEAR KTEGARINVA KQQVEAALNA RRQALADALL NQRLAAEAID VTLPGRGAGA GSLHPVMRTW ERVEQIFRSI GFDVADGPEI ETDWYNFTSL NSPENHPARS MQDTFYVEGK DADGRQLLLR THTSPMQVRY ARMNRPPIKV IAPGRTYRVD SDATHSPMFN QVEGLWIDEN ISFADLKGVY TDFLKKFFER DDILVRFRPS YFPFTEPSAE IDMMFEHGKN AGKWLEISGS GQVHPTVIRN MGLDPERYIG FAFGSGLERL TMLRYGVQDL RLFFENDLRF LRQFA
NT Sequence
ATGACGTTGG AAATGATGGG ATCTATGGAT CTGGACCAGA TTGTCGCCGA CGCGCAGCAG TCCTTCGAAC AGGCTGCCGA CATCACCACG CTCGAAAACG AGAAAGCACG ATTTCTCGGC AAGTCGGGTG CGCTGACCGA GTTGCTCAAG GGCCTCGGCA AGCTCGATCC CGAAGCACGC AAGACCGAAG GCGCGCGCAT CAACGTTGCG AAGCAGCAGG TTGAAGCCGC GCTGAACGCA CGTCGCCAGG CGCTCGCCGA CGCGCTGTTG AACCAGCGCC TCGCCGCCGA GGCGATCGAC GTCACGCTGC CGGGTCGCGG CGCCGGTGCA GGCAGCCTGC ATCCGGTGAT GCGCACGTGG GAGCGCGTCG AACAGATTTT CCGCTCGATC GGCTTCGACG TAGCCGACGG TCCCGAAATC GAGACCGACT GGTACAACTT CACGTCGCTG AACAGCCCGG AGAACCATCC GGCGCGTTCG ATGCAGGATA CCTTCTACGT CGAAGGCAAG GATGCCGACG GCCGCCAGCT GCTGCTGCGC ACGCACACGA GCCCGATGCA GGTGCGTTAC GCGCGCATGA ACCGTCCGCC GATCAAGGTC ATCGCGCCGG GCCGCACGTA TCGCGTGGAC AGCGATGCGA CGCACTCGCC GATGTTCAAT CAGGTCGAGG GGCTGTGGAT CGACGAAAAC ATCAGCTTCG CCGACCTCAA GGGCGTCTAT ACCGATTTCC TGAAGAAATT CTTCGAGCGC GACGACATCC TCGTGCGCTT CCGTCCGTCG TATTTCCCGT TCACGGAACC GTCGGCCGAG ATCGACATGA TGTTCGAGCA CGGCAAGAAC GCCGGCAAAT GGCTCGAGAT CTCGGGCTCG GGGCAGGTGC ATCCGACCGT GATCCGCAAC ATGGGCCTCG ATCCCGAGCG CTACATCGGC TTTGCGTTCG GCAGCGGCCT CGAGCGCCTG ACGATGCTGC GCTACGGTGT GCAGGATCTC CGGCTGTTCT TCGAGAACGA TCTGCGTTTC CTGCGCCAGT TCGCA
Details for BuamA.00163.a.B1.GE39142
HARVESTED ON: 5/6/2015
SEQUENCED ON: 5/6/2015
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMT LEMMGSMDLD QIVADAQQSX EQAADITTLE NEKARFLGKS GALTELLKGL GKLDPEARKT EGARINVAKQ QVEAALNARR QALADALLNQ RLAAEAIDVT LPGRGAGAGS LHPVMRTWER VEQIFRSIGF DVADGPEIET DWYNFTSLNS PENHPARSMQ DTFYVEGKDA DGRQLLLRTH TSPMQVRYAR MNRPPIKVIA PGRTYRVDSD ATHSPMFNQV EGLWIDENIS FADLKGVYTD FLKKFFERDD ILVRFRPSYF PFTEPSAEID MMFEHGKNAG KWLEISGSGQ VHPTVIRNMG LDPERYIGFA FGSGLERLTM LRYGVQDLRL FFEN
Validated NT Sequence
ancgttctcg aagaacagcc ggagatcctg cacaccgtag cgcagcatcg tcaggcgctc gaggccgctg ccgaacgcaa agccgatgta gcgctcggga tcgaggccca tgttgcggat cacggtcgga tgcacctgcc ccgagcccga gatctcgagc catttgccgg cgttcttgcc gtgctcgaac atcatgtcga tctcggccga cggttccgtg aacgggaaat acgacggacg gaagcgcacg aggatgtcgt cgcgctcgaa gaatttcttc aggaaatcgg tatagacgcc cttgaggtcg gcgaagctga tgttttcgtc gatccacagc ccctcgacct gattgaacat cggcgagtgc gtcgcatcgc tgtccacgcg atacgtgcgg cccggcgcga tgaccttgat cggcggacgg ttcatgcgcg cgtaacgcac ctgcatcggg ctcgtgtgcg tgcgcagcag cagctggcgg ccgtcggcat ccttgccttc gacgtagaag gtatcctgca tcgaacgcgc cggatggttc tccgggctgt tcagcgacgt gaagttgtac cagtcggtct cgatttcggg accgtcggct acgtcgaagc cgatcgagcg gaaaatctgt tcgacgcgct cccacgtgcg catcaccgga tgcaggctgc ctgcaccggc gccgcgaccc ggcagcgtga cgtcgatcgc ctcggcggcg aggcgctggt tcaacagcgc gtcggcgagc gcctggcgac gtgcgttcag cgcggcttca acctgctgct tcgcaacgtt gatgcgcgcg ccttcggtct tgcgtgcttc gggatcgagc ttgccgaggc ccttgagcaa ctcggtcagc gcacccgact tgccgagaaa tcgtgctttc tcgttttcga gcgtggtgat gtcggcagcc tgttcnaagg actgctgcgc gtcggcgaca atctggtcca gatccataga tcccatcatt tccaacgtca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMT LEMMGSMDLD QIVADAQQSF EQAADITTLE NEKARFLGKS GALTELLKGL GKLDPEARKT EGARINVAKQ QVEAALNARR QALADALLNQ RLAAEAIDVT LPGRGAGAGS LHPVMRTWER VEQIFRSIGF DVADGPEIET DWYNFTSLNS PENHPARSMQ DTFYVEGKDA DGRQLLLRTH TSPMQVRYAR MNRPPIKVIA PGRTYRVDSD ATHSPMFNQV EGLWIDENIS FADLKGVYTD FLKKFFERDD ILVRFRPSYF PFTEPSAEID MMFEHGKNAG KWLEISGSGQ VHPTVIRNMG LDPERYIGFA FGSGLERLTM LRYGVQDLRL FFENDLRFLR QFA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacgt tggaaatgat gggatctatg gatctggacc agattgtcgc cgacgcgcag cagtccttcg aacaggctgc cgacatcacc acgctcgaaa acgagaaagc acgatttctc ggcaagtcgg gtgcgctgac cgagttgctc aagggcctcg gcaagctcga tcccgaagca cgcaagaccg aaggcgcgcg catcaacgtt gcgaagcagc aggttgaagc cgcgctgaac gcacgtcgcc aggcgctcgc cgacgcgctg ttgaaccagc gcctcgccgc cgaggcgatc gacgtcacgc tgccgggtcg cggcgccggt gcaggcagcc tgcatccggt gatgcgcacg tgggagcgcg tcgaacagat tttccgctcg atcggcttcg acgtagccga cggtcccgaa atcgagaccg actggtacaa cttcacgtcg ctgaacagcc cggagaacca tccggcgcgt tcgatgcagg ataccttcta cgtcgaaggc aaggatgccg acggccgcca gctgctgctg cgcacgcaca cgagcccgat gcaggtgcgt tacgcgcgca tgaaccgtcc gccgatcaag gtcatcgcgc cgggccgcac gtatcgcgtg gacagcgatg cgacgcactc gccgatgttc aatcaggtcg aggggctgtg gatcgacgaa aacatcagct tcgccgacct caagggcgtc tataccgatt tcctgaagaa attcttcgag cgcgacgaca tcctcgtgcg cttccgtccg tcgtatttcc cgttcacgga accgtcggcc gagatcgaca tgatgttcga gcacggcaag aacgccggca aatggctcga gatctcgggc tcggggcagg tgcatccgac cgtgatccgc aacatgggcc tcgatcccga gcgctacatc ggctttgcgt tcggcagcgg cctcgagcgc ctgacgatgc tgcgctacgg tgtgcaggat ctccggctgt tcttcgagaa cgatctgcgt ttcctgcgcc agttcgcatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat