BuamA.00158.a

Glutamate racemase

CENTER ID: BuamA.00158.a
ORGANISM: Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.00158.a.B1.GE39795 Full length( BuamA.00158.a ) 1 287
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.00158.a.B1.PS37907 Full length( BuamA.00158.a ) 1 287

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|398577.6.peg.2168
RefSeq: YP_001808809.1
UniProt: B1YTA4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTMTHQPAAS GVRPAAPVGI FDSGLGGLSV LRAVRAHLPG ESFVYVADSQ HAPYGPRDEA FITERTLAIG EWLAREGAKA LVIACNTATA RAIAAIRERL SIPLVGVEPG IKPAAALSTT GVAGVLATQS TLNSPRFQAL LDRYGAGRRF ICQPGHGLVE AIERGDTNSP ALRTLLERYL QPMLDDGADT LVLGCTHYPF FTETIRDIVG DRLTIVDTSD AIARQLARVL DERGLRAPAG TRAAPPRFCS TGDGRQLHAL ASTLLGLDAP VESVTISSPN ARACATA
NT Sequence
ATGACGATGA CGCACCAGCC AGCCGCTTCC GGGGTCCGCC CCGCCGCCCC CGTCGGGATC TTCGATTCGG GGCTCGGCGG CCTGTCGGTG CTGCGCGCCG TGCGCGCGCA CCTGCCCGGC GAATCGTTCG TCTACGTCGC CGATTCGCAG CACGCGCCGT ACGGGCCGCG CGACGAGGCA TTCATTACGG AACGCACGCT GGCCATCGGC GAGTGGCTCG CCCGCGAAGG GGCGAAAGCG CTCGTGATCG CGTGCAACAC GGCAACGGCG CGAGCGATCG CGGCCATCCG CGAACGCCTG TCGATACCGC TCGTCGGTGT CGAGCCGGGC ATCAAACCGG CGGCAGCGCT GTCCACGACC GGCGTCGCGG GCGTCCTCGC GACGCAATCG ACGCTGAACA GCCCGCGTTT CCAGGCGCTG CTCGACCGCT ACGGCGCCGG CCGCCGCTTC ATCTGCCAGC CGGGCCACGG GCTCGTCGAA GCGATCGAAC GCGGCGACAC GAACTCGCCC GCATTGCGCA CGTTGCTCGA GCGTTATCTG CAGCCGATGC TCGACGACGG TGCCGATACG CTGGTGCTCG GCTGCACGCA CTATCCGTTC TTCACAGAAA CGATTCGTGA CATTGTTGGC GACCGCCTGA CGATCGTCGA CACGAGCGAT GCCATCGCCC GCCAGCTCGC GCGGGTGCTC GACGAACGCG GCCTGCGCGC GCCGGCCGGC ACGCGTGCCG CGCCGCCGCG CTTTTGCTCG ACGGGCGACG GCCGGCAACT GCACGCACTC GCGTCGACGC TGCTTGGCCT CGACGCGCCG GTCGAATCCG TCACCATTTC CTCGCCGAAC GCGCGCGCCT GCGCGACCGC C
Details for BuamA.00158.a.B1.GE39795
HARVESTED ON: 2/4/2016
SEQUENCED ON: 1/27/2016
EXPECTED MW: 31kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMT MTHQPAASGV RPAAPVGIFD SGLGGLSVLR AVRAHLPGES FVYVADSQHA PYGPRDEAFI TERTLAIGEW LAREGAKALV IACNTATARA IAAIRERLSI PLVGVEPGIK PAAALSTTGV AGVLATQSTL NSPRFQALLD RYGAGRRFIC QPGHGLVEAI ERGDTNSPAL RTLLERYLQP MLDDGADTLV LGCTHYPFFT ETIRDIVGDR LTIVDTSDAI ARQLARVLDE RGLRAPAGTR AAPPRFCSTG DGRQLHALAS TLLGLDAPVE SVTISSPXXR ACATA
Validated NT Sequence
atcttactca cttaggcggt cgcgcaggcg cgcnnnntcg gcgaggaaat ggtgacggat tcgaccggcg cgtcgaggcc aagcagcgtc gacgcgagtg cgtgcagttg ccggccgtcg cccgtcgagc aaaagcgcgg cggcgcggca cgcgtgccgg ccggcgcgcg caggccgcgt tcgtcgagca cccgcgcgag ctggcgggcg atggcatcgc tcgtgtcgac gatcgtcagg cggtcgccaa caatgtcacg aatcgtttct gtgaagaacg gatagtgcgt gcagccgagc accagcgtat cggcaccgtc gtcgagcatc ggctgcagat aacgctcgag caacgtgcgc aatgcgggcg agttcgtgtc gccgcgttcg atcgcttcga cgagcccgtg gcccggctgg cagatgaagc ggcggccggc gccgtagcgg tcgagcagcg cctggaaacg cgggctgttc agcgtcgatt gcgtcgcgag gacgcccgcg acgccggtcg tggacagcgc tgccgccggt ttgatgcccg gctcgacacc gacgagcggt atcgacaggc gttcgcggat ggccgcgatc gctcgcgccg ttgccgtgtt gcacgcgatc acgagcgctt tcgccccttc gcgggcgagc cactcgccga tggccagcgt gcgttccgta atgaatgcct cgtcgcgcgg cccgtacggc gcgtgctgcg aatcggcgac gtagacgaac gattcgccgg gcaggtgcgc gcgcacggcg cgcagcaccg acaggccgcc gagccccgaa tcgaagatcc cgacgggggc ggcggggcgg accccggaag cggctggctg gtgcgtcatc gtcatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHMT MTHQPAASGV RPAAPVGIFD SGLGGLSVLR AVRAHLPGES FVYVADSQHA PYGPRDEAFI TERTLAIGEW LAREGAKALV IACNTATARA IAAIRERLSI PLVGVEPGIK PAAALSTTGV AGVLATQSTL NSPRFQALLD RYGAGRRFIC QPGHGLVEAI ERGDTNSPAL RTLLERYLQP MLDDGADTLV LGCTHYPFFT ETIRDIVGDR LTIVDTSDAI ARQLARVLDE RGLRAPAGTR AAPPRFCSTG DGRQLHALAS TLLGLDAPVE SVTISSPNAR ACATA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacga tgacgcacca gccagccgct tccggggtcc gccccgccgc ccccgtcggg atcttcgatt cggggctcgg cggcctgtcg gtgctgcgcg ccgtgcgcgc gcacctgccc ggcgaatcgt tcgtctacgt cgccgattcg cagcacgcgc cgtacgggcc gcgcgacgag gcattcatta cggaacgcac gctggccatc ggcgagtggc tcgcccgcga aggggcgaaa gcgctcgtga tcgcgtgcaa cacggcaacg gcgcgagcga tcgcggccat ccgcgaacgc ctgtcgatac cgctcgtcgg tgtcgagccg ggcatcaaac cggcggcagc gctgtccacg accggcgtcg cgggcgtcct cgcgacgcaa tcgacgctga acagcccgcg tttccaggcg ctgctcgacc gctacggcgc cggccgccgc ttcatctgcc agccgggcca cgggctcgtc gaagcgatcg aacgcggcga cacgaactcg cccgcattgc gcacgttgct cgagcgttat ctgcagccga tgctcgacga cggtgccgat acgctggtgc tcggctgcac gcactatccg ttcttcacag aaacgattcg tgacattgtt ggcgaccgcc tgacgatcgt cgacacgagc gatgccatcg cccgccagct cgcgcgggtg ctcgacgaac gcggcctgcg cgcgccggcc ggcacgcgtg ccgcgccgcc gcgcttttgc tcgacgggcg acggccggca actgcacgca ctcgcgtcga cgctgcttgg cctcgacgcg ccggtcgaat ccgtcaccat ttcctcgccg aacgcgcgcg cctgcgcgac cgcctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for BuamA.00158.a.B1.PS37907
PURIFICATION DATe: 4/12/2016
CONCENTRATION: 48.2mg/ml
OBSERVED MW: 30kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC Buffer
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 9
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMT MTHQPAASGV RPAAPVGIFD SGLGGLSVLR AVRAHLPGES FVYVADSQHA PYGPRDEAFI TERTLAIGEW LAREGAKALV IACNTATARA IAAIRERLSI PLVGVEPGIK PAAALSTTGV AGVLATQSTL NSPRFQALLD RYGAGRRFIC QPGHGLVEAI ERGDTNSPAL RTLLERYLQP MLDDGADTLV LGCTHYPFFT ETIRDIVGDR LTIVDTSDAI ARQLARVLDE RGLRAPAGTR AAPPRFCSTG DGRQLHALAS TLLGLDAPVE SVTISSPXXR ACATA
Validated NT Sequence
atcttactca cttaggcggt cgcgcaggcg cgcnnnntcg gcgaggaaat ggtgacggat tcgaccggcg cgtcgaggcc aagcagcgtc gacgcgagtg cgtgcagttg ccggccgtcg cccgtcgagc aaaagcgcgg cggcgcggca cgcgtgccgg ccggcgcgcg caggccgcgt tcgtcgagca cccgcgcgag ctggcgggcg atggcatcgc tcgtgtcgac gatcgtcagg cggtcgccaa caatgtcacg aatcgtttct gtgaagaacg gatagtgcgt gcagccgagc accagcgtat cggcaccgtc gtcgagcatc ggctgcagat aacgctcgag caacgtgcgc aatgcgggcg agttcgtgtc gccgcgttcg atcgcttcga cgagcccgtg gcccggctgg cagatgaagc ggcggccggc gccgtagcgg tcgagcagcg cctggaaacg cgggctgttc agcgtcgatt gcgtcgcgag gacgcccgcg acgccggtcg tggacagcgc tgccgccggt ttgatgcccg gctcgacacc gacgagcggt atcgacaggc gttcgcggat ggccgcgatc gctcgcgccg ttgccgtgtt gcacgcgatc acgagcgctt tcgccccttc gcgggcgagc cactcgccga tggccagcgt gcgttccgta atgaatgcct cgtcgcgcgg cccgtacggc gcgtgctgcg aatcggcgac gtagacgaac gattcgccgg gcaggtgcgc gcgcacggcg cgcagcaccg acaggccgcc gagccccgaa tcgaagatcc cgacgggggc ggcggggcgg accccggaag cggctggctg gtgcgtcatc gtcatatggt ggtggtggtg gtgagccat
Expressed Protein Sequence
MAHHHHHHMT MTHQPAASGV RPAAPVGIFD SGLGGLSVLR AVRAHLPGES FVYVADSQHA PYGPRDEAFI TERTLAIGEW LAREGAKALV IACNTATARA IAAIRERLSI PLVGVEPGIK PAAALSTTGV AGVLATQSTL NSPRFQALLD RYGAGRRFIC QPGHGLVEAI ERGDTNSPAL RTLLERYLQP MLDDGADTLV LGCTHYPFFT ETIRDIVGDR LTIVDTSDAI ARQLARVLDE RGLRAPAGTR AAPPRFCSTG DGRQLHALAS TLLGLDAPVE SVTISSPNAR ACATA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACGA TGACGCACCA GCCAGCCGCT TCCGGGGTCC GCCCCGCCGC CCCCGTCGGG ATCTTCGATT CGGGGCTCGG CGGCCTGTCG GTGCTGCGCG CCGTGCGCGC GCACCTGCCC GGCGAATCGT TCGTCTACGT CGCCGATTCG CAGCACGCGC CGTACGGGCC GCGCGACGAG GCATTCATTA CGGAACGCAC GCTGGCCATC GGCGAGTGGC TCGCCCGCGA AGGGGCGAAA GCGCTCGTGA TCGCGTGCAA CACGGCAACG GCGCGAGCGA TCGCGGCCAT CCGCGAACGC CTGTCGATAC CGCTCGTCGG TGTCGAGCCG GGCATCAAAC CGGCGGCAGC GCTGTCCACG ACCGGCGTCG CGGGCGTCCT CGCGACGCAA TCGACGCTGA ACAGCCCGCG TTTCCAGGCG CTGCTCGACC GCTACGGCGC CGGCCGCCGC TTCATCTGCC AGCCGGGCCA CGGGCTCGTC GAAGCGATCG AACGCGGCGA CACGAACTCG CCCGCATTGC GCACGTTGCT CGAGCGTTAT CTGCAGCCGA TGCTCGACGA CGGTGCCGAT ACGCTGGTGC TCGGCTGCAC GCACTATCCG TTCTTCACAG AAACGATTCG TGACATTGTT GGCGACCGCC TGACGATCGT CGACACGAGC GATGCCATCG CCCGCCAGCT CGCGCGGGTG CTCGACGAAC GCGGCCTGCG CGCGCCGGCC GGCACGCGTG CCGCGCCGCC GCGCTTTTGC TCGACGGGCG ACGGCCGGCA ACTGCACGCA CTCGCGTCGA CGCTGCTTGG CCTCGACGCG CCGGTCGAAT CCGTCACCAT TTCCTCGCCG AACGCGCGCG CCTGCGCGAC CGCCTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT