SSGCID: Target Page

BuamA.00148.b

Tyrosine recombinase XerD

CENTER ID:	BuamA.00148.b
ORGANISM:	Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuamA.00148.b.B1.GE39792	Full length( BuamA.00148.b )		1	322

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|398577.6.peg.2549
RefSeq:	YP_001809172.1
UniProt:	B1YVF2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSEPLLSPDS ASDTDTAGDA AAASPVLLAS RASIDVFCDA LWLEHGLARN TLDAYRRDLV LFSQWLAAMH DAPLDSADEA MLIGYIAARS DGKATSSNRR LSVFRRYYGW AVREHRANAD PTLRITSAKQ AARFPSTLSE AQVEALLGAP DVGTPLGLRD RTMLELMYAS GLRVSELVTL KTVEVGLNEG VVRVMGKGSK ERLVPFGEVA HGWIERYLRD ARPALLGARA ADALFVTARG DGMTRQQFWN IIKRHAQQAD VRAHLSPHTL RHAFATHLLN HGADLRVVQL LLGHSDISTT QIYTHVARER LKTLHATHHP RG

NT Sequence

ATGAGTGAAC CGTTGCTTTC CCCCGACTCC GCGTCCGACA CCGATACCGC TGGCGACGCG GCCGCCGCAT CGCCCGTGCT GCTCGCGAGC CGCGCGTCGA TCGACGTGTT CTGCGATGCG CTGTGGCTCG AACACGGGCT CGCGCGCAAT ACGCTCGATG CGTACCGGCG CGATCTGGTG CTGTTTTCGC AATGGCTGGC CGCGATGCAC GACGCGCCGC TCGACTCGGC CGACGAAGCG ATGTTGATCG GCTACATCGC CGCGCGCAGC GACGGCAAGG CGACATCGTC GAACCGGCGC TTGTCGGTGT TCCGGCGCTA TTACGGCTGG GCCGTGCGCG AGCATCGCGC GAATGCGGAC CCGACGCTGC GGATCACGTC GGCGAAGCAG GCCGCGCGGT TTCCGTCGAC GCTGTCCGAG GCGCAGGTCG AGGCGCTGCT CGGTGCACCC GACGTCGGCA CGCCGCTCGG CCTGCGCGAT CGCACGATGC TCGAGCTGAT GTATGCAAGC GGGCTGCGCG TGAGCGAGCT CGTCACGCTG AAGACCGTCG AGGTCGGGCT CAACGAGGGC GTCGTGCGCG TGATGGGCAA GGGCTCGAAG GAACGGCTCG TGCCGTTCGG CGAAGTCGCG CACGGCTGGA TCGAGCGCTA CCTGCGCGAC GCGCGGCCGG CGCTGCTCGG CGCACGCGCG GCCGACGCGC TGTTCGTGAC CGCGCGCGGC GACGGGATGA CGCGCCAGCA GTTCTGGAAC ATCATCAAGC GTCACGCGCA GCAGGCCGAC GTGCGCGCGC ACCTGTCGCC GCATACGCTG CGGCACGCGT TCGCGACGCA CCTGCTGAAC CACGGCGCCG ACCTGCGCGT CGTGCAGCTG CTGCTCGGTC ACAGCGATAT CTCGACCACG CAGATCTACA CGCACGTCGC GCGCGAGCGG CTCAAGACGC TGCACGCGAC GCACCATCCG CGCGGC

Details for BuamA.00148.b.B1.GE39792

HARVESTED ON:	2/4/2016
SEQUENCED ON:	1/27/2016
EXPECTED MW:	36kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMS EPLLSPDSAS DTDTAGDAAA ASPVLLASRA SIDVFCDALW LEHGLARNTL DAYRRDLVLF SQWLAAMHDA PLDSADEAML IGYIAARSDG KATSSNRRLS VFRRYYGWAV REHRANADPT LRITSAKQAA RFPSTLSEAQ VEALLGAPDV GTPLGLRDRT MLELMYASGL RVSELVTLKT VEVGLNEGVV RVMGKGSKER LVPFGEVAHG WIERYLRDAR PALLGARAAD ALFVTARGDG MTRQQFWNII KRHAQQADVR AHLSPHTLRH AFATHLLNHG ADLRVVQLLL GHSDISTTQI YTHVARERLK T

Validated NT Sequence

cagcgtcttg agccgctcgc gcgcgacgtg cgtgtagatc tgcgtggtcg agatatcgct gtgaccgagc agcagctgca cgacgcgcag gtcggcgccg tggttcagca ggtgcgtcgc gaacgcgtgc cgcagcgtat gcggcgacag gtgcgcgcgc acgtcggcct gctgcgcgtg acgcttgatg atgttccaga actgctggcg cgtcatcccg tcgccgcgcg cggtcacgaa cagcgcgtcg gccgcgcgtg cgccgagcag cgccggccgc gcgtcgcgca ggtagcgctc gatccagccg tgcgcgactt cgccgaacgg cacgagccgt tccttcgagc ccttgcccat cacgcgcacg acgccctcgt tgagcccgac ctcgacggtc ttcagcgtga cgagctcgct cacgcgcagc ccgcttgcat acatcagctc gagcatcgtg cgatcgcgca ggccgagcgg cgtgccgacg tcgggtgcac cgagcagcgc ctcgacctgc gcctcggaca gcgtcgacgg aaaccgcgcg gcctgcttcg ccgacgtgat ccgcagcgtc gggtccgcat tcgcgcgatg ctcgcgcacg gcccagccgt aatagcgccg gaacaccgac aagcgccggt tcgacgatgt cgccttgccg tcgctgcgcg cggcgatgta gccgatcaac atcgcttcgt cggccgagtc gagcggcgcg tcgtgcatcg cggccagcca ttgcgaaaac agcaccagat cgcgccggta cgcatcgagc gtattgcgcg cgagcccgtg ttcgagccac agcgcatcgc agaacacgtc gatcgacgcg cggctcgcga gcagcacggg cgatgcggcg gccgcgtcgc cagcggtatc ggtgtcggac gcggagtcgg gggaaagcaa cggttcactc atatggtggt ggtggtggtg agccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagtg aaccgttgct ttcccccgac tccgcgtccg acaccgatac cgctggcgac gcggccgccg catcgcccgt gctgctcgcg agccgcgcgt cgatcgacgt gttctgcgat gcgctgtggc tcgaacacgg gctcgcgcgc aatacgctcg atgcgtaccg gcgcgatctg gtgctgtttt cgcaatggct ggccgcgatg cacgacgcgc cgctcgactc ggccgacgaa gcgatgttga tcggctacat cgccgcgcgc agcgacggca aggcgacatc gtcgaaccgg cgcttgtcgg tgttccggcg ctattacggc tgggccgtgc gcgagcatcg cgcgaatgcg gacccgacgc tgcggatcac gtcggcgaag caggccgcgc ggtttccgtc gacgctgtcc gaggcgcagg tcgaggcgct gctcggtgca cccgacgtcg gcacgccgct cggcctgcgc gatcgcacga tgctcgagct gatgtatgca agcgggctgc gcgtgagcga gctcgtcacg ctgaagaccg tcgaggtcgg gctcaacgag ggcgtcgtgc gcgtgatggg caagggctcg aaggaacggc tcgtgccgtt cggcgaagtc gcgcacggct ggatcgagcg ctacctgcgc gacgcgcggc cggcgctgct cggcgcacgc gcggccgacg cgctgttcgt gaccgcgcgc ggcgacggga tgacgcgcca gcagttctgg aacatcatca agcgtcacgc gcagcaggcc gacgtgcgcg cgcacctgtc gccgcatacg ctgcggcacg cgttcgcgac gcacctgctg aaccacggcg ccgacctgcg cgtcgtgcag ctgctgctcg gtcacagcga tatctcgacc acgcagatct acacgcacgt cgcgcgcgag cggctcaaga cgctgcacgc gacgcaccat ccgcgcggct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat