BuamA.00148.a

Tyrosine recombinase XerC

CENTER ID:	BuamA.00148.a
ORGANISM:	Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuamA.00148.a.B1.GE39791	Full length( BuamA.00148.a )		1	306

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_137884
PATRIC ID:	fig\|398577.6.peg.3099
RefSeq:	YP_001809688.1
UniProt:	B1YPQ8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTDDPIAAYL SNLKHVRQLS EHTLRAYTHE LDELKKLAAG RPLDTLTAVD MRGAVARAHA GGLSARSISH RLSAWRAFYR WLAQHIEMPA NPVAAVRAPK RPKTLPKALS VDDASALMDA PLAGTTEGIR DHAILELFYS SGLRLAELVG LDVRYTQADG YRSAGWLDLA EAEVTVRGKG NKERKVPVGR KAIDALNAWL AVRGEFVKHD PHPLFVSVRG NRMAPGVVRE RVKRAALAAG IPANVHPHVL RHSFATHVLQ SSGDLRAVQE LLGHASISAT QVYTSLDFQH LAKIYDSAHP RAKKRD

NT Sequence

ATGACCGACG ATCCGATCGC CGCCTACCTG TCGAACCTGA AGCACGTCAG GCAGCTGTCG GAACACACGC TGCGTGCGTA CACGCATGAA CTCGACGAAC TGAAGAAGCT CGCCGCCGGT CGCCCGCTCG ACACGCTGAC GGCCGTCGAC ATGCGCGGCG CGGTCGCGCG CGCGCATGCG GGCGGATTGT CGGCGCGCTC GATCTCGCAC CGGCTGTCCG CCTGGCGCGC GTTCTACCGC TGGCTCGCGC AGCACATCGA GATGCCCGCG AACCCGGTCG CCGCCGTGCG CGCGCCGAAG CGCCCGAAAA CCTTGCCGAA AGCGCTGTCG GTCGACGACG CCTCGGCGCT GATGGACGCG CCGCTCGCCG GCACGACCGA AGGCATCCGC GATCACGCGA TCCTCGAGCT GTTCTATTCG TCGGGGCTGC GCCTCGCCGA ACTGGTCGGG CTCGACGTCA GGTACACGCA GGCCGACGGC TACCGCTCGG CCGGCTGGCT CGATCTCGCC GAAGCCGAAG TCACCGTGCG CGGCAAGGGC AACAAGGAAC GCAAGGTGCC GGTCGGCCGC AAGGCGATCG ATGCGCTGAA CGCGTGGCTC GCGGTGCGCG GCGAATTCGT GAAGCACGAC CCGCATCCGC TGTTCGTGTC CGTGCGCGGC AACCGGATGG CGCCGGGCGT CGTGCGCGAG CGCGTGAAGC GCGCGGCGCT CGCCGCGGGC ATCCCCGCCA ACGTCCACCC GCACGTGCTG CGCCATTCGT TCGCGACGCA CGTGCTGCAG TCGAGCGGCG ACTTGCGCGC GGTGCAGGAA CTGCTGGGCC ACGCGAGCAT CTCCGCGACG CAGGTCTACA CGTCGCTCGA CTTCCAGCAC CTCGCGAAGA TTTACGACAG CGCGCATCCG CGCGCGAAGA AGCGCGAC

Details for BuamA.00148.a.B1.GE39791

HARVESTED ON:	data unavailable
SEQUENCED ON:	1/27/2016
EXPECTED MW:	35kDa
OBSERVED MW:	23kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMT DDPIAAYLSN LKHVRQLSEH TLRAYTHELD ELKKLAAGRP LDTLTAVDMR GAVARAHAGG LSARSISHRL SAWRAFYRWL AQHIEMPANP VAAVRAPKRP KTLPKALSVD DASALMDAPL AGTTEGIRDH AILELFYSSG LRLAELVGLD VRYTQADGYR SAGWLDLAEA EVTVRGKGNK ERKVPVGRKA IDALNAWLAV RGEFVKHDPH PLFVSVRGNR MAPGVVRERV KRAALAAGIP ANVHPHVLRH SFATHVLQSS GDLRAVQELL GHASISATQV YTSLDFQHLA KIYDSAXXRA KKRD

Validated NT Sequence

ttactcactt agtcgcgctt cttcgcgcgn nnnnncgcgc tgtcgtaaat cttcgcgagg tgctggaagt cgagcgacgt gtagacctgc gtcgcggaga tgctcgcgtg gcccagcagt tcctgcaccg cgcgcaagtc gccgctcgac tgcagcacgt gcgtcgcgaa cgaatggcgc agcacgtgcg ggtggacgtt ggcggggatg cccgcggcga gcgccgcgcg cttcacgcgc tcgcgcacga cgcccggcgc catccggttg ccgcgcacgg acacgaacag cggatgcggg tcgtgcttca cgaattcgcc gcgcaccgcg agccacgcgt tcagcgcatc gatcgccttg cggccgaccg gcaccttgcg ttccttgttg cccttgccgc gcacggtgac ttcggcttcg gcgagatcga gccagccggc cgagcggtag ccgtcggcct gcgtgtacct gacgtcgagc ccgaccagtt cggcgaggcg cagccccgac gaatagaaca gctcgaggat cgcgtgatcg cggatgcctt cggtcgtgcc ggcgagcggc gcgtccatca gcgccgaggc gtcgtcgacc gacagcgctt tcggcaaggt tttcgggcgc ttcggcgcgc gcacggcggc gaccgggttc gcgggcatct cgatgtgctg cgcgagccag cggtagaacg cgcgccaggc ggacagccgg tgcgagatcg agcgcgccga caatccgccc gcatgcgcgc gcgcgaccgc gccgcgcatg tcgacggccg tcagcgtgtc gagcgggcga ccggcggcga gcttcttcag ttcgtcgagt tcatgcgtgt acgcacgcag cgtgtgttcc gacagctgcc tgacgtgctt caggttcgac aggtaggcgg cgatcggatc gtcggtcata tggtggtggt ggtggtgagc catggtatat c

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaccg acgatccgat cgccgcctac ctgtcgaacc tgaagcacgt caggcagctg tcggaacaca cgctgcgtgc gtacacgcat gaactcgacg aactgaagaa gctcgccgcc ggtcgcccgc tcgacacgct gacggccgtc gacatgcgcg gcgcggtcgc gcgcgcgcat gcgggcggat tgtcggcgcg ctcgatctcg caccggctgt ccgcctggcg cgcgttctac cgctggctcg cgcagcacat cgagatgccc gcgaacccgg tcgccgccgt gcgcgcgccg aagcgcccga aaaccttgcc gaaagcgctg tcggtcgacg acgcctcggc gctgatggac gcgccgctcg ccggcacgac cgaaggcatc cgcgatcacg cgatcctcga gctgttctat tcgtcggggc tgcgcctcgc cgaactggtc gggctcgacg tcaggtacac gcaggccgac ggctaccgct cggccggctg gctcgatctc gccgaagccg aagtcaccgt gcgcggcaag ggcaacaagg aacgcaaggt gccggtcggc cgcaaggcga tcgatgcgct gaacgcgtgg ctcgcggtgc gcggcgaatt cgtgaagcac gacccgcatc cgctgttcgt gtccgtgcgc ggcaaccgga tggcgccggg cgtcgtgcgc gagcgcgtga agcgcgcggc gctcgccgcg ggcatccccg ccaacgtcca cccgcacgtg ctgcgccatt cgttcgcgac gcacgtgctg cagtcgagcg gcgacttgcg cgcggtgcag gaactgctgg gccacgcgag catctccgcg acgcaggtct acacgtcgct cgacttccag cacctcgcga agatttacga cagcgcgcat ccgcgcgcga agaagcgcga ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat