BuamA.00137.b

UDP-N-acetylmuramate

CENTER ID: BuamA.00137.b
ORGANISM: Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.00137.b.B2.GE43968 Mature protein( BuamA.00137.b ) 20 461
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|398577.6.peg.535
RefSeq: YP_001807248.1
UniProt: B1YSX1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHIHILGICG TFMGGLAVLA REAGHTVTGC DAGVYPPMST QLEAQGITLI EGYGAEQIDL KPDLFVIGNV VTRGNPLMEA ILDRGLPYVS GPQWLGEHVL AGKWVLAVAG THGKTTTSSM LAWLLEDAGL NPGFLIGGVP LNFGVSARLT DSSFFVIEAD EYDTAFFDKR SKFVHYRPRT AVLNNLEFDH ADIFPDLAAI ETQFHHLVRT VPGVGRLVTN GRSDALERVL ARGCWSEVER FGVDGGWQAL PAEDGVPVDE RFAVYSQSER VGEVAWQVQG DHNRMNALAA IAAARHVGVP PAQAAESLAS FRNVKRRMEV RGSIDGVTVY DDFAHHPTAI ETTIAGLRAR IGRQNSRILA VLEPRSNTMK LGVMKAQLPA SLADADLVFG YGAPAGRDVL GWNLADALAP LGDKARAFDD LHLLVKSVVE AARPGDHVLV MSNGGFGGVH QKLLDALGSR A
NT Sequence
ATGCACATCC ACATTCTTGG CATCTGCGGC ACCTTCATGG GCGGTCTTGC CGTGCTCGCG CGCGAGGCGG GCCACACGGT GACGGGTTGC GACGCGGGCG TCTATCCGCC GATGAGCACG CAGCTCGAGG CGCAGGGCAT CACGCTGATC GAGGGCTACG GCGCCGAACA GATCGACCTG AAACCCGACC TGTTCGTGAT CGGCAACGTC GTCACGCGCG GCAACCCGCT GATGGAGGCG ATCCTCGATC GCGGCCTGCC TTACGTATCC GGCCCGCAAT GGCTCGGCGA GCACGTGCTG GCCGGCAAGT GGGTGCTGGC GGTCGCGGGC ACGCACGGCA AGACGACCAC GTCGTCGATG CTCGCGTGGC TGCTGGAAGA TGCCGGCCTG AACCCGGGCT TCCTGATCGG CGGCGTGCCG CTGAATTTCG GCGTGTCGGC GCGGCTGACC GATTCGAGCT TCTTCGTGAT CGAGGCCGAC GAATACGATA CGGCGTTCTT CGACAAGCGC TCGAAGTTCG TCCACTACCG GCCGCGCACC GCGGTGCTCA ACAATCTCGA ATTCGATCAC GCGGACATCT TCCCGGATCT CGCCGCGATC GAAACGCAAT TCCATCATCT CGTACGCACG GTGCCGGGTG TCGGCCGGCT CGTGACCAAC GGCCGTTCCG ACGCGCTGGA GCGCGTGCTC GCGCGCGGCT GCTGGAGCGA GGTCGAGCGC TTTGGCGTCG ACGGCGGCTG GCAGGCGCTG CCGGCCGAGG ACGGCGTGCC GGTCGACGAA CGGTTCGCGG TGTATTCGCA GTCGGAGCGC GTCGGTGAAG TCGCGTGGCA GGTGCAGGGC GACCACAACC GGATGAACGC GCTCGCGGCG ATCGCCGCCG CGCGCCACGT CGGCGTGCCG CCCGCCCAGG CCGCCGAATC ACTCGCATCG TTTCGCAACG TGAAGCGGCG CATGGAAGTG CGCGGCAGCA TCGACGGCGT GACCGTGTAC GACGACTTCG CGCACCATCC GACCGCGATC GAAACCACGA TCGCCGGCCT TCGTGCGCGC ATCGGCCGCC AAAATTCCCG CATTCTCGCG GTGCTCGAGC CGCGCTCGAA CACGATGAAG CTGGGCGTGA TGAAGGCGCA ACTGCCGGCC AGCCTCGCGG ACGCCGATCT GGTGTTCGGC TATGGCGCGC CTGCCGGGCG CGACGTGCTG GGCTGGAACC TGGCCGACGC GCTCGCGCCG CTCGGCGACA AGGCGCGTGC GTTCGACGAT CTCCATCTGC TGGTGAAGTC GGTCGTGGAG GCCGCGCGCC CGGGCGACCA CGTGCTGGTG ATGAGCAACG GCGGCTTCGG CGGCGTGCAC CAGAAGCTGC TCGACGCGCT CGGGAGCCGC GCG
Details for BuamA.00137.b.B2.GE43968
HARVESTED ON: 12/3/2020
SEQUENCED ON: 12/16/2020
EXPECTED MW: 49kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMA REAGHTVTGC DAGVYPPMST QLEAQGITLI EGYGAEQIDL KPDLFVIGNV VTRGNPLMEA ILDRGLPYVS GPQWLGEHVL AGKWVLAVAG THGKTTTSSM LAWLLEDAGL NPGFLIGGVP LNFGVSARLT DSSFFVIEAD EYDTAFFDKR SKFVHYRPRT AVLNNLEFDH ADIFPDLAAI ETQFHHLVRT VPGVGRLVTN GRSDALERVL ARGCWSEVER FGVDGGWQAL PAEDGVPVDE RFAVYSQSER VGEVAWQVQG DHNRMNALAA IAAARHVGVP PAQAAESLAS FRNVKRRMEV RGSIDGVTVY DDFAHHPTAI ETTIAGLRAR IGRQNSRILA VLEPRSNTMK LGVMKAQLPA SLADADLVFG YGAPAGRDVL GWNLADALAP LGDKARAFDD LHLLVKSVVE AARPGDHVLV MSNGGFGGVH QKLL
Validated NT Sequence
atggctcacc accaccacca ccatatggcg cgcgaggcgg gccacacggt gacgggttgc gacgcgggcg tctatccgcc gatgagcacg cagctcgagg cgcagggcat cacgctgatc gagggctacg gcgccgaaca gatcgacctg aaacccgacc tgttcgtgat cggcaacgtc gtcacgcgcg gcaacccgct gatggaggcg atcctcgatc gcggcctgcc ttacgtatcc ggcccgcaat ggctcggcga gcacgtgctg gccggcaagt gggtgctggc ggtcgcgggc acgcacggca agacgaccac gtcgtcgatg ctcgcgtggc tgctggaaga tgccggcctg aacccgggct tcctgatcgg cggcgtgccg ctgaatttcg gcgtgtcggc gcggctgacc gattcgagct tcttcgtgat cgaggccgac gaatacgata cggcgttctt cgacaagcgc tcgaagttcg tccactaccg gccgcgcacc gcggtgctca acaatctcga attcgatcac gcggacatct tcccggatct cgccgcgatc gaaacgcaat tccatcatct cgtacgcacg gtgccgggtg tcggccggct cgtgaccaac ggccgttccg acgcgctgga gcgcgtgctc gcgcgcggct gctggagcga ggtcgagcgc tttggcgtcg acggcggctg gcaggcgctg ccggccgagg acggcgtgcc ggtcgacgaa cggttcgcgg tgtattcgca gtcggagcgc gtcggtgaag tcgcgtggca ggtgcagggc gaccacaacc ggatgaacgc gctcgcggcg atcgccgccg cgcgccacgt cggcgtgccg cccgcccagg ccgccgaatc actcgcatcg tttcgcaacg tgaagcggcg catggaagtg cgcggcagca tcgacggcgt gaccgtgtac gacgacttcg cgcaccatcc gaccgcgatc gaaaccacga tcgccggcct tcgtgcgcgc atcggccgcc aaaattcccg cattctcgcg gtgctcgagc cgcgctcgaa cacgatgaag ctgggcgtga tgaaggcgca actgccggcc agcctcgcgg acgccgatct ggtgttcggc tatggcgcgc ctgccgggcg cgacgtgctg ggctggaacc tggccgacgc gctcgcgccg ctcggcgaca aggcgcgtgc gttcgacgat ctccatctgc tggtgaagtc ggtcgtggag gccgcgcgcc cgggcgacca cgtgctggtg atgagcaacg gcggcttcgg cggcgtgcac cagaagctgc tng
Expected Protein Sequence
MAHHHHHHAR EAGHTVTGCD AGVYPPMSTQ LEAQGITLIE GYGAEQIDLK PDLFVIGNVV TRGNPLMEAI LDRGLPYVSG PQWLGEHVLA GKWVLAVAGT HGKTTTSSML AWLLEDAGLN PGFLIGGVPL NFGVSARLTD SSFFVIEADE YDTAFFDKRS KFVHYRPRTA VLNNLEFDHA DIFPDLAAIE TQFHHLVRTV PGVGRLVTNG RSDALERVLA RGCWSEVERF GVDGGWQALP AEDGVPVDER FAVYSQSERV GEVAWQVQGD HNRMNALAAI AAARHVGVPP AQAAESLASF RNVKRRMEVR GSIDGVTVYD DFAHHPTAIE TTIAGLRARI GRQNSRILAV LEPRSNTMKL GVMKAQLPAS LADADLVFGY GAPAGRDVLG WNLADALAPL GDKARAFDDL HLLVKSVVEA ARPGDHVLVM SNGGFGGVHQ KLLDALGSRA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcgcgcg aggcgggcca cacggtgacg ggttgcgacg cgggcgtcta tccgccgatg agcacgcagc tcgaggcgca gggcatcacg ctgatcgagg gctacggcgc cgaacagatc gacctgaaac ccgacctgtt cgtgatcggc aacgtcgtca cgcgcggcaa cccgctgatg gaggcgatcc tcgatcgcgg cctgccttac gtatccggcc cgcaatggct cggcgagcac gtgctggccg gcaagtgggt gctggcggtc gcgggcacgc acggcaagac gaccacgtcg tcgatgctcg cgtggctgct ggaagatgcc ggcctgaacc cgggcttcct gatcggcggc gtgccgctga atttcggcgt gtcggcgcgg ctgaccgatt cgagcttctt cgtgatcgag gccgacgaat acgatacggc gttcttcgac aagcgctcga agttcgtcca ctaccggccg cgcaccgcgg tgctcaacaa tctcgaattc gatcacgcgg acatcttccc ggatctcgcc gcgatcgaaa cgcaattcca tcatctcgta cgcacggtgc cgggtgtcgg ccggctcgtg accaacggcc gttccgacgc gctggagcgc gtgctcgcgc gcggctgctg gagcgaggtc gagcgctttg gcgtcgacgg cggctggcag gcgctgccgg ccgaggacgg cgtgccggtc gacgaacggt tcgcggtgta ttcgcagtcg gagcgcgtcg gtgaagtcgc gtggcaggtg cagggcgacc acaaccggat gaacgcgctc gcggcgatcg ccgccgcgcg ccacgtcggc gtgccgcccg cccaggccgc cgaatcactc gcatcgtttc gcaacgtgaa gcggcgcatg gaagtgcgcg gcagcatcga cggcgtgacc gtgtacgacg acttcgcgca ccatccgacc gcgatcgaaa ccacgatcgc cggccttcgt gcgcgcatcg gccgccaaaa ttcccgcatt ctcgcggtgc tcgagccgcg ctcgaacacg atgaagctgg gcgtgatgaa ggcgcaactg ccggccagcc tcgcggacgc cgatctggtg ttcggctatg gcgcgcctgc cgggcgcgac gtgctgggct ggaacctggc cgacgcgctc gcgccgctcg gcgacaaggc gcgtgcgttc gacgatctcc atctgctggt gaagtcggtc gtggaggccg cgcgcccggg cgaccacgtg ctggtgatga gcaacggcgg cttcggcggc gtgcaccaga agctgctcga cgcgctcggg agccgcgcgt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat