BuamA.00137.a

UDP-N-acetylmuramate--L-alanine ligase

CENTER ID: BuamA.00137.a
ORGANISM: Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.00137.a.B1.GE43967 Full length( BuamA.00137.a ) 1 465
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|398577.6.peg.484
RefSeq: YP_001807202.1
UniProt: B1YSS5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKHIVKQIHF VGIGGAGMSG IAEVLVNLGY EVSGSDLSRN AVTDRLEALG ARIAIGHDAA NIAGANAVVV STAVRSDNPE VLAARHQGVP IVQRAVMLAE LMRLKQGIAI AGTHGKTTTT SLVASVLAAG GLDPTFVIGG RLISAGANAR LGTGDFIVAE ADESDASFLN LYPVIEVITN IDADHMDTYG HDFARLKQAF IEFTQRLPFY GSAVVCVDDP NVRQIIPFIS KPVVRYGLSP DAQVRAEDID ARDGRMHFTV IREGRAPLAV VLNLPGLHNV QNALAAIAIA TDLGVSDDAI QQALAEFNGV GRRFQRYGEV PSADGGQYTL IDDYGHHPVE MAATVAAARG AFPGRRLVLA FQPHRYTRTR DCFDDFVNVL STVDALVLTE VYAAGEAAIA TANGDALSRA LRAVGKVDPV FVASVDDVPD ALAGVARNGD VVITMGAGSI GGVPAKIVQN TQQKE
NT Sequence
ATGAAACACA TCGTCAAACA GATTCATTTC GTCGGGATCG GCGGCGCGGG CATGAGCGGC ATCGCCGAAG TGCTCGTCAA TCTCGGCTAC GAGGTCAGCG GCTCGGACCT GTCGCGCAAC GCGGTGACCG ATCGCCTCGA GGCGCTCGGC GCGCGGATCG CGATCGGTCA CGATGCAGCG AACATCGCGG GCGCGAACGC GGTCGTCGTG TCGACGGCCG TGCGCTCCGA CAATCCCGAA GTACTGGCCG CACGGCACCA AGGTGTGCCG ATCGTGCAGC GCGCGGTGAT GCTCGCGGAG CTGATGCGCC TGAAGCAGGG GATCGCGATT GCCGGCACGC ACGGCAAGAC CACGACGACG AGCCTCGTCG CGAGCGTGCT CGCGGCGGGC GGCCTCGATC CGACCTTCGT GATCGGCGGC CGGCTGATCA GCGCCGGCGC GAACGCCCGG CTCGGCACGG GCGACTTCAT CGTCGCCGAG GCCGACGAGT CGGACGCGTC GTTCCTGAAC CTGTATCCGG TGATCGAAGT CATCACGAAC ATCGACGCCG ACCACATGGA CACCTACGGC CACGATTTCG CGCGGCTCAA GCAGGCGTTC ATCGAGTTCA CGCAGCGCCT GCCGTTCTAC GGCAGCGCGG TCGTGTGCGT CGACGATCCG AACGTGCGGC AGATCATCCC GTTCATCTCG AAGCCGGTCG TGCGCTACGG CCTGTCGCCG GATGCGCAGG TGCGCGCGGA AGACATCGAC GCGCGCGACG GCCGCATGCA CTTCACGGTG ATCCGCGAAG GTCGTGCGCC GCTCGCGGTG GTGCTGAACC TGCCGGGCCT GCACAACGTG CAGAACGCGC TCGCGGCGAT CGCGATCGCG ACCGATCTCG GCGTGTCGGA CGACGCGATC CAGCAGGCGC TGGCGGAATT CAACGGCGTC GGCCGGCGCT TCCAGCGCTA CGGCGAGGTG CCGAGCGCGG ACGGCGGCCA GTACACGCTG ATCGACGACT ACGGCCACCA CCCGGTCGAG ATGGCCGCGA CGGTCGCGGC CGCGCGCGGC GCGTTTCCGG GCCGCCGCCT CGTGCTGGCG TTCCAGCCGC ACCGCTATAC GCGCACGCGC GACTGCTTCG ACGACTTCGT CAACGTGCTG TCGACGGTCG ACGCGCTGGT GCTGACGGAA GTCTATGCCG CGGGCGAAGC GGCGATCGCG ACGGCCAACG GCGACGCGCT GTCGCGCGCG CTGCGTGCGG TGGGGAAGGT CGACCCGGTG TTCGTCGCGT CGGTCGACGA CGTGCCGGAC GCACTGGCGG GGGTCGCTCG GAACGGCGAC GTGGTGATCA CGATGGGCGC GGGTTCGATC GGCGGCGTGC CGGCGAAGAT CGTGCAGAAC ACGCAACAGA AGGAA
Details for BuamA.00137.a.B1.GE43967
HARVESTED ON: 12/3/2020
SEQUENCED ON: 12/16/2020
EXPECTED MW: 50kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMK HIVKQIHFVG IGGAGMSGIA EVLVNLGYEV SGSDLSRNAV TDRLEALGAR IAIGHDAANI AGANAVVVST AVRSDNPEVL AARHQGVPIV QRAVMLAELM RLKQGIAIAG THGKTTTTSL VASVLAAGGL DPTFVIGGRL ISAGANARLG TGDFIVAEAD ESDASFLNLY PVIEVITNID ADHMDTYGHD FARLKQAFIE FTQRLPFYGS AVVCVDDPNV RQIIPFISKP VVRYGLSPDA QVRAEDIDAR DGRMHFTVIR EGRAPLAVVL NLPGLHNVQN ALAAIAIATD LGVSDDAIQQ ALAEFNGVGR RFQRYGEVPS ADGGQYTLID DYGHHPVEMA ATVAAARGAF PGRRLVLAFQ PHRYTRTRDC FDDFVNVLST VDALVLTEVY AAGEAAIATA NGDALSRALR AVGKVDPVFV ASVDDVPDAL AGVARNGDVV ITMGAGSIGG VPAKIVQNTQ QKE
Validated NT Sequence
atggctcacc accaccacca ccatatgaaa cacatcgtca aacagattca tttcgtcggg atcggcggcg cgggcatgag cggcatcgcc gaagtgctcg tcaatctcgg ctacgaggtc agcggctcgg acctgtcgcg caacgcggtg accgatcgcc tcgaggcgct cggcgcgcgg atcgcgatcg gtcacgatgc agcgaacatc gcgggcgcga acgcggtcgt cgtgtcgacg gccgtgcgct ccgacaatcc cgaagtactg gccgcacggc accaaggtgt gccgatcgtg cagcgcgcgg tgatgctcgc ggagctgatg cgcctgaagc aggggatcgc gattgccggc acgcacggca agaccacgac gacgagcctc gtcgcgagcg tgctcgcggc gggcggcctc gatccgacct tcgtgatcgg cggccggctg atcagcgccg gcgcgaacgc ccggctcggc acgggcgact tcatcgtcgc cgaggccgac gagtcggacg cgtcgttcct gaacctgtat ccggtgatcg aagtcatcac gaacatcgac gccgaccaca tggacaccta cggccacgat ttcgcgcggc tcaagcaggc gttcatcgag ttcacgcagc gcctgccgtt ctacggcagc gcggtcgtgt gcgtcgacga tccgaacgtg cggcagatca tcccgttcat ctcgaagccg gtcgtgcgct acggcctgtc gccggatgcg caggtgcgcg cggnaagaca tcgacgcgcg cgacggccgc atgcacttca cggtgatccg cgaaggtcgt gcgccgctcg cggtggtgct gaacctgccg ggcctgcaca acgtgcagaa cgcgctcgcg gcgatcgcga tcgcgaccga tctcggcgtg tcggacgacg cgatccagca ggcgctggcg gaattcaacg gcgtcggccg gcgcttccag cgctacggcg aggtgccgag cgcggacggc ggccagtaca cgctgatcga cgactacggc caccacccgg tcgagatggc cgcgacggtc gcggccgcgc gcggcgcgtt tccgggccgc cgcctcgtgc tggcgttcca gccgcaccgc tatacgcgca cgcgcgactg cttcgacgac ttcgtcaacg tgctgtcgac ggtcgacgcg ctggtgctga cggaagtcta tgccgcgggc gaagcggcga tcgcgacggc caacggcgac gcgctgtcgc gcgcgctgcg tgcggtgggg aaggtcgacc cggtgttcgt cgcgtcggtc gacgacgtgc cggacgcact ggcgggggtc gctcggaacg gcgacgtggt gatcacgatg ggcgcgggtt cgatcggcgg cgtgccggcg aagatcgtgc agaac
Expected Protein Sequence
MAHHHHHHMK HIVKQIHFVG IGGAGMSGIA EVLVNLGYEV SGSDLSRNAV TDRLEALGAR IAIGHDAANI AGANAVVVST AVRSDNPEVL AARHQGVPIV QRAVMLAELM RLKQGIAIAG THGKTTTTSL VASVLAAGGL DPTFVIGGRL ISAGANARLG TGDFIVAEAD ESDASFLNLY PVIEVITNID ADHMDTYGHD FARLKQAFIE FTQRLPFYGS AVVCVDDPNV RQIIPFISKP VVRYGLSPDA QVRAEDIDAR DGRMHFTVIR EGRAPLAVVL NLPGLHNVQN ALAAIAIATD LGVSDDAIQQ ALAEFNGVGR RFQRYGEVPS ADGGQYTLID DYGHHPVEMA ATVAAARGAF PGRRLVLAFQ PHRYTRTRDC FDDFVNVLST VDALVLTEVY AAGEAAIATA NGDALSRALR AVGKVDPVFV ASVDDVPDAL AGVARNGDVV ITMGAGSIGG VPAKIVQNTQ QKE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaac acatcgtcaa acagattcat ttcgtcggga tcggcggcgc gggcatgagc ggcatcgccg aagtgctcgt caatctcggc tacgaggtca gcggctcgga cctgtcgcgc aacgcggtga ccgatcgcct cgaggcgctc ggcgcgcgga tcgcgatcgg tcacgatgca gcgaacatcg cgggcgcgaa cgcggtcgtc gtgtcgacgg ccgtgcgctc cgacaatccc gaagtactgg ccgcacggca ccaaggtgtg ccgatcgtgc agcgcgcggt gatgctcgcg gagctgatgc gcctgaagca ggggatcgcg attgccggca cgcacggcaa gaccacgacg acgagcctcg tcgcgagcgt gctcgcggcg ggcggcctcg atccgacctt cgtgatcggc ggccggctga tcagcgccgg cgcgaacgcc cggctcggca cgggcgactt catcgtcgcc gaggccgacg agtcggacgc gtcgttcctg aacctgtatc cggtgatcga agtcatcacg aacatcgacg ccgaccacat ggacacctac ggccacgatt tcgcgcggct caagcaggcg ttcatcgagt tcacgcagcg cctgccgttc tacggcagcg cggtcgtgtg cgtcgacgat ccgaacgtgc ggcagatcat cccgttcatc tcgaagccgg tcgtgcgcta cggcctgtcg ccggatgcgc aggtgcgcgc ggaagacatc gacgcgcgcg acggccgcat gcacttcacg gtgatccgcg aaggtcgtgc gccgctcgcg gtggtgctga acctgccggg cctgcacaac gtgcagaacg cgctcgcggc gatcgcgatc gcgaccgatc tcggcgtgtc ggacgacgcg atccagcagg cgctggcgga attcaacggc gtcggccggc gcttccagcg ctacggcgag gtgccgagcg cggacggcgg ccagtacacg ctgatcgacg actacggcca ccacccggtc gagatggccg cgacggtcgc ggccgcgcgc ggcgcgtttc cgggccgccg cctcgtgctg gcgttccagc cgcaccgcta tacgcgcacg cgcgactgct tcgacgactt cgtcaacgtg ctgtcgacgg tcgacgcgct ggtgctgacg gaagtctatg ccgcgggcga agcggcgatc gcgacggcca acggcgacgc gctgtcgcgc gcgctgcgtg cggtggggaa ggtcgacccg gtgttcgtcg cgtcggtcga cgacgtgccg gacgcactgg cgggggtcgc tcggaacggc gacgtggtga tcacgatggg cgcgggttcg atcggcggcg tgccggcgaa gatcgtgcag aacacgcaac agaaggaatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat