BuamA.00134.a

4-hydroxyphenylpyruvate dioxygenase

CENTER ID: BuamA.00134.a
ORGANISM: Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.00134.a.B1.GE39788 Full length( BuamA.00134.a ) 1 375
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.00134.a.B1.PS37910 Full length( BuamA.00134.a ) 1 375

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|398577.6.peg.4418
RefSeq: YP_001810954.1
UniProt: B1YWM6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPGNPHPLSS DTPPVADPAA NPLGMAGLEF VEFAAPVPDA LAQRFEQLGF KAIARHVSKN VTLYRQGQMQ FLINAEPESF AARYAEEYGM GVCAIGLRVA SARRAFERAI ELGAWAFEGE RIGVGELKIP AIQGIGDSHL YFVDRWRGRD GQRGGVGDIS IFDIDFRPID IATAHTDHDC VGTGLQQVDH FTQTVGAGRM REWLDFYHDL LHFREIHEID AHWHVSEESR VMVSPCGAVR IPVYEEGTRR TELMHAYLPD HPGEGVQHVA LSTDDILASV DALRANGVEF IEPPARYYDE VDARLPGHGV DLDALRRRAV LVDGEIGSDG VPRLFFQTFV KRRPGEIFFE IVQRQGHHGF GEGNLAALAR ARDAG
NT Sequence
ATGCCCGGCA ATCCCCACCC CCTGTCCAGC GATACGCCGC CCGTCGCCGA TCCGGCCGCC AATCCGCTCG GGATGGCCGG ACTCGAATTC GTCGAATTCG CGGCGCCGGT GCCGGACGCG CTCGCGCAAC GCTTCGAGCA GCTCGGGTTC AAGGCGATCG CGCGCCATGT GAGCAAGAAC GTCACGCTGT ACCGGCAAGG GCAGATGCAA TTCCTGATCA ACGCCGAGCC CGAATCGTTC GCGGCGCGCT ATGCGGAGGA ATACGGGATG GGCGTCTGCG CGATCGGGCT GCGCGTGGCG AGCGCGCGGC GCGCGTTCGA GCGGGCGATC GAACTGGGCG CGTGGGCGTT CGAGGGCGAG CGGATCGGCG TCGGCGAGCT GAAGATTCCC GCGATCCAGG GCATCGGCGA TTCGCATCTC TATTTCGTCG ACCGCTGGCG CGGGCGGGAT GGCCAGCGCG GCGGCGTCGG CGACATCTCG ATTTTCGACA TCGATTTCCG GCCGATCGAC ATCGCGACCG CCCATACCGA TCACGATTGC GTGGGCACGG GCCTGCAGCA AGTCGACCAC TTCACGCAGA CGGTCGGCGC GGGGCGCATG CGCGAATGGC TCGACTTCTA CCATGACCTG CTGCACTTCC GCGAAATCCA TGAAATCGAT GCGCACTGGC ATGTATCGGA AGAATCGCGC GTGATGGTGT CGCCGTGCGG CGCGGTGCGG ATTCCCGTGT ACGAGGAAGG CACGCGGCGC ACCGAGCTGA TGCACGCGTA TCTGCCGGAC CATCCCGGCG AAGGCGTCCA GCACGTTGCG CTGTCGACCG ACGACATCCT GGCGAGCGTC GATGCGCTGC GCGCGAACGG CGTCGAATTC ATCGAGCCGC CGGCGCGCTA TTACGACGAA GTCGATGCGC GGCTGCCCGG GCACGGCGTC GATCTCGACG CGCTGCGCCG CCGCGCGGTG CTGGTCGACG GCGAAATCGG CAGCGATGGC GTGCCGCGGC TGTTCTTCCA GACCTTCGTC AAGCGCCGGC CCGGCGAGAT CTTCTTCGAG ATCGTGCAGC GGCAAGGGCA CCACGGATTC GGGGAAGGGA ACCTCGCGGC GCTGGCCCGC GCCCGCGACG CGGGC
Details for BuamA.00134.a.B1.GE39788
HARVESTED ON: 2/4/2016
SEQUENCED ON: 1/27/2016
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMP GNPHPLSSDT PPVADPAANP LGMAGLEFVE FAAPVPDALA QRFEQLGFKA IARHVSKNVT LYRQGQMQFL INAEPESFAA RYAEEYGMGV CAIGLRVASA RRAFERAIEL GAWAFEGERI GVGELKIPAI QGIGDSHLYF VDRWRGRDGQ RGGVGDISIF DIDFRPIDIA TAHTDHDCVG TGLQQVDHFT QTVGAGRMRE WLDFYHDLLH FREIHEIDAH WHVSEESRVM VSPCGAVRIP VYEEGTRRTE LMHAYLPDHP GEGVQHVALS TDDILASVDA LRANGVEFIE PPARYYDEVD ARLPGHGVDL DALRRRAVLV DGEIGSDGVP RLFFQTFVKR RPGEIFFEIV QRQGHHGFGE GNLA
Validated NT Sequence
gccgcgaggt tcccttcccc gaatccgtgg tgcccttgcc gctgcacgat ctcgaagaag atctcgccgg gccggcgctt gacgaaggtc tggaagaaca gccgcggcac gccatcgctg ccgatttcgc cgtcgaccag caccgcgcgg cggcgcagcg cgtcgagatc gacgccgtgc ccgggcagcc gcgcatcgac ttcgtcgtaa tagcgcgccg gcggctcgat gaattcgacg ccgttcgcgc gcagcgcatc gacgctcgcc aggatgtcgt cggtcgacag cgcaacgtgc tggacgcctt cgccgggatg gtccggcaga tacgcgtgca tcagctcggt gcgccgcgtg ccttcctcgt acacgggaat ccgcaccgcg ccgcacggcg acaccatcac gcgcgattct tccgatacat gccagtgcgc atcgatttca tggatttcgc ggaagtgcag caggtcatgg tagaagtcga gccattcgcg catgcgcccc gcgccgaccg tctgcgtgaa gtggtcgact tgctgcaggc ccgtgcccac gcaatcgtga tcggtatggg cggtcgcgat gtcgatcggc cggaaatcga tgtcgaaaat cgagatgtcg ccgacgccgc cgcgctggcc atcccgcccg cgccagcggt cgacgaaata gagatgcgaa tcgccgatgc cctggatcgc gggaatcttc agctcgccga cgccgatccg ctcgccctcg aacgcccacg cgcccagttc gatcgcccgc tcgaacgcgc gccgcgcgct cgccacgcgc agcccgatcg cgcagacgcc catcccgtat tcctccgcat agcgcgccgc gaacgattcg ggctcggcgt tgatcaggaa ttgcatctgc ccttgccggt acagcgtgac gttcttgctc acatggcgcg cgatcgcctt gaacccgagc tgctcgaagc gttgcgcgag cgcgtccggc accggcgccg cgaattcgac gaattcgagt ccggccatcc cgagcggatt ggcggccgga tcggcgacgg gcggcgtatc gctggacagg gggtggggat tgccgggcat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHMP GNPHPLSSDT PPVADPAANP LGMAGLEFVE FAAPVPDALA QRFEQLGFKA IARHVSKNVT LYRQGQMQFL INAEPESFAA RYAEEYGMGV CAIGLRVASA RRAFERAIEL GAWAFEGERI GVGELKIPAI QGIGDSHLYF VDRWRGRDGQ RGGVGDISIF DIDFRPIDIA TAHTDHDCVG TGLQQVDHFT QTVGAGRMRE WLDFYHDLLH FREIHEIDAH WHVSEESRVM VSPCGAVRIP VYEEGTRRTE LMHAYLPDHP GEGVQHVALS TDDILASVDA LRANGVEFIE PPARYYDEVD ARLPGHGVDL DALRRRAVLV DGEIGSDGVP RLFFQTFVKR RPGEIFFEIV QRQGHHGFGE GNLAALARAR DAG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcccg gcaatcccca ccccctgtcc agcgatacgc cgcccgtcgc cgatccggcc gccaatccgc tcgggatggc cggactcgaa ttcgtcgaat tcgcggcgcc ggtgccggac gcgctcgcgc aacgcttcga gcagctcggg ttcaaggcga tcgcgcgcca tgtgagcaag aacgtcacgc tgtaccggca agggcagatg caattcctga tcaacgccga gcccgaatcg ttcgcggcgc gctatgcgga ggaatacggg atgggcgtct gcgcgatcgg gctgcgcgtg gcgagcgcgc ggcgcgcgtt cgagcgggcg atcgaactgg gcgcgtgggc gttcgagggc gagcggatcg gcgtcggcga gctgaagatt cccgcgatcc agggcatcgg cgattcgcat ctctatttcg tcgaccgctg gcgcgggcgg gatggccagc gcggcggcgt cggcgacatc tcgattttcg acatcgattt ccggccgatc gacatcgcga ccgcccatac cgatcacgat tgcgtgggca cgggcctgca gcaagtcgac cacttcacgc agacggtcgg cgcggggcgc atgcgcgaat ggctcgactt ctaccatgac ctgctgcact tccgcgaaat ccatgaaatc gatgcgcact ggcatgtatc ggaagaatcg cgcgtgatgg tgtcgccgtg cggcgcggtg cggattcccg tgtacgagga aggcacgcgg cgcaccgagc tgatgcacgc gtatctgccg gaccatcccg gcgaaggcgt ccagcacgtt gcgctgtcga ccgacgacat cctggcgagc gtcgatgcgc tgcgcgcgaa cggcgtcgaa ttcatcgagc cgccggcgcg ctattacgac gaagtcgatg cgcggctgcc cgggcacggc gtcgatctcg acgcgctgcg ccgccgcgcg gtgctggtcg acggcgaaat cggcagcgat ggcgtgccgc ggctgttctt ccagaccttc gtcaagcgcc ggcccggcga gatcttcttc gagatcgtgc agcggcaagg gcaccacgga ttcggggaag ggaacctcgc ggcgctggcc cgcgcccgcg acgcgggctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for BuamA.00134.a.B1.PS37910
PURIFICATION DATe: 4/12/2016
CONCENTRATION: 24.97mg/ml
OBSERVED MW: 40kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: SEC
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMP GNPHPLSSDT PPVADPAANP LGMAGLEFVE FAAPVPDALA QRFEQLGFKA IARHVSKNVT LYRQGQMQFL INAEPESFAA RYAEEYGMGV CAIGLRVASA RRAFERAIEL GAWAFEGERI GVGELKIPAI QGIGDSHLYF VDRWRGRDGQ RGGVGDISIF DIDFRPIDIA TAHTDHDCVG TGLQQVDHFT QTVGAGRMRE WLDFYHDLLH FREIHEIDAH WHVSEESRVM VSPCGAVRIP VYEEGTRRTE LMHAYLPDHP GEGVQHVALS TDDILASVDA LRANGVEFIE PPARYYDEVD ARLPGHGVDL DALRRRAVLV DGEIGSDGVP RLFFQTFVKR RPGEIFFEIV QRQGHHGFGE GNLA
Validated NT Sequence
gccgcgaggt tcccttcccc gaatccgtgg tgcccttgcc gctgcacgat ctcgaagaag atctcgccgg gccggcgctt gacgaaggtc tggaagaaca gccgcggcac gccatcgctg ccgatttcgc cgtcgaccag caccgcgcgg cggcgcagcg cgtcgagatc gacgccgtgc ccgggcagcc gcgcatcgac ttcgtcgtaa tagcgcgccg gcggctcgat gaattcgacg ccgttcgcgc gcagcgcatc gacgctcgcc aggatgtcgt cggtcgacag cgcaacgtgc tggacgcctt cgccgggatg gtccggcaga tacgcgtgca tcagctcggt gcgccgcgtg ccttcctcgt acacgggaat ccgcaccgcg ccgcacggcg acaccatcac gcgcgattct tccgatacat gccagtgcgc atcgatttca tggatttcgc ggaagtgcag caggtcatgg tagaagtcga gccattcgcg catgcgcccc gcgccgaccg tctgcgtgaa gtggtcgact tgctgcaggc ccgtgcccac gcaatcgtga tcggtatggg cggtcgcgat gtcgatcggc cggaaatcga tgtcgaaaat cgagatgtcg ccgacgccgc cgcgctggcc atcccgcccg cgccagcggt cgacgaaata gagatgcgaa tcgccgatgc cctggatcgc gggaatcttc agctcgccga cgccgatccg ctcgccctcg aacgcccacg cgcccagttc gatcgcccgc tcgaacgcgc gccgcgcgct cgccacgcgc agcccgatcg cgcagacgcc catcccgtat tcctccgcat agcgcgccgc gaacgattcg ggctcggcgt tgatcaggaa ttgcatctgc ccttgccggt acagcgtgac gttcttgctc acatggcgcg cgatcgcctt gaacccgagc tgctcgaagc gttgcgcgag cgcgtccggc accggcgccg cgaattcgac gaattcgagt ccggccatcc cgagcggatt ggcggccgga tcggcgacgg gcggcgtatc gctggacagg gggtggggat tgccgggcat atggtggtgg tggtggtgag ccat
Expressed Protein Sequence
MAHHHHHHMP GNPHPLSSDT PPVADPAANP LGMAGLEFVE FAAPVPDALA QRFEQLGFKA IARHVSKNVT LYRQGQMQFL INAEPESFAA RYAEEYGMGV CAIGLRVASA RRAFERAIEL GAWAFEGERI GVGELKIPAI QGIGDSHLYF VDRWRGRDGQ RGGVGDISIF DIDFRPIDIA TAHTDHDCVG TGLQQVDHFT QTVGAGRMRE WLDFYHDLLH FREIHEIDAH WHVSEESRVM VSPCGAVRIP VYEEGTRRTE LMHAYLPDHP GEGVQHVALS TDDILASVDA LRANGVEFIE PPARYYDEVD ARLPGHGVDL DALRRRAVLV DGEIGSDGVP RLFFQTFVKR RPGEIFFEIV QRQGHHGFGE GNLAALARAR DAG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCCCG GCAATCCCCA CCCCCTGTCC AGCGATACGC CGCCCGTCGC CGATCCGGCC GCCAATCCGC TCGGGATGGC CGGACTCGAA TTCGTCGAAT TCGCGGCGCC GGTGCCGGAC GCGCTCGCGC AACGCTTCGA GCAGCTCGGG TTCAAGGCGA TCGCGCGCCA TGTGAGCAAG AACGTCACGC TGTACCGGCA AGGGCAGATG CAATTCCTGA TCAACGCCGA GCCCGAATCG TTCGCGGCGC GCTATGCGGA GGAATACGGG ATGGGCGTCT GCGCGATCGG GCTGCGCGTG GCGAGCGCGC GGCGCGCGTT CGAGCGGGCG ATCGAACTGG GCGCGTGGGC GTTCGAGGGC GAGCGGATCG GCGTCGGCGA GCTGAAGATT CCCGCGATCC AGGGCATCGG CGATTCGCAT CTCTATTTCG TCGACCGCTG GCGCGGGCGG GATGGCCAGC GCGGCGGCGT CGGCGACATC TCGATTTTCG ACATCGATTT CCGGCCGATC GACATCGCGA CCGCCCATAC CGATCACGAT TGCGTGGGCA CGGGCCTGCA GCAAGTCGAC CACTTCACGC AGACGGTCGG CGCGGGGCGC ATGCGCGAAT GGCTCGACTT CTACCATGAC CTGCTGCACT TCCGCGAAAT CCATGAAATC GATGCGCACT GGCATGTATC GGAAGAATCG CGCGTGATGG TGTCGCCGTG CGGCGCGGTG CGGATTCCCG TGTACGAGGA AGGCACGCGG CGCACCGAGC TGATGCACGC GTATCTGCCG GACCATCCCG GCGAAGGCGT CCAGCACGTT GCGCTGTCGA CCGACGACAT CCTGGCGAGC GTCGATGCGC TGCGCGCGAA CGGCGTCGAA TTCATCGAGC CGCCGGCGCG CTATTACGAC GAAGTCGATG CGCGGCTGCC CGGGCACGGC GTCGATCTCG ACGCGCTGCG CCGCCGCGCG GTGCTGGTCG ACGGCGAAAT CGGCAGCGAT GGCGTGCCGC GGCTGTTCTT CCAGACCTTC GTCAAGCGCC GGCCCGGCGA GATCTTCTTC GAGATCGTGC AGCGGCAAGG GCACCACGGA TTCGGGGAAG GGAACCTCGC GGCGCTGGCC CGCGCCCGCG ACGCGGGCTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT