BuamA.00120.a

GTPase obg

CENTER ID: BuamA.00120.a
ORGANISM: Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.00120.a.A1.GE32128 Full length( BuamA.00120.a ) 1 370
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.00120.a.A1.PS01122 Full length( BuamA.00120.a ) 1 370

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|398577.6.peg.507
RefSeq: YP_001807224.1
UniProt: B1YSU7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKFIDEARIE VIAGDGGDGS ASMRREKFVP FGGPDGGDGG RGGNVYAIAD RNINTLIDYR YAKKHLARNG ENGRGSDCYG KGGDDVTLRM PVGTVVTDMD TGELIADLTE HGQQVMLAQG GAGGLGNLHF KSSTNRAPRQ KTDGKPGERR MLRLELKVLA DVGLLGMPNA GKSTFISSVS NAKPKIADYP FTTLAPNLGV VRVGPSKSFV IADIPGLIEG AAEGAGLGHQ FLRHLQRTGV LLHLVDLAPF DENVDPVAEA KAIVGELRKY DEALYEKPRW LVLNKLDMVP EDEREARVAD FLARFEWDGP VFEISALTGQ GCEALCYAIY DYLAEHSDAH RAAEEEDLAT DVRFRDAPPA DGGATPGGDA
NT Sequence
ATGAAGTTCA TTGACGAAGC ACGAATCGAA GTCATCGCCG GCGACGGAGG TGATGGGAGC GCGTCGATGC GCCGCGAAAA GTTCGTTCCG TTCGGCGGGC CGGACGGCGG CGACGGCGGC CGGGGCGGCA ACGTCTACGC GATTGCCGAC CGCAACATCA ACACGCTGAT CGACTACCGT TACGCGAAGA AGCACCTGGC GCGCAACGGC GAAAACGGTC GCGGCTCGGA TTGCTACGGC AAGGGCGGTG ACGACGTCAC GCTGCGCATG CCGGTCGGCA CGGTCGTGAC CGACATGGAT ACCGGCGAGC TGATCGCCGA CCTGACCGAG CACGGCCAGC AAGTGATGCT TGCGCAAGGC GGTGCGGGCG GCCTCGGCAA CCTGCATTTC AAGTCGAGCA CGAACCGTGC GCCGCGCCAG AAGACCGACG GCAAGCCGGG CGAACGGCGC ATGCTGCGGC TCGAGCTGAA GGTGCTCGCC GACGTCGGGC TGCTCGGCAT GCCGAACGCC GGCAAGTCGA CCTTCATCTC GTCGGTGTCG AACGCGAAGC CGAAGATCGC CGACTATCCG TTTACGACGC TCGCGCCGAA CCTCGGCGTC GTGCGGGTCG GCCCGAGCAA GAGCTTCGTG ATCGCCGACA TCCCGGGGCT GATCGAAGGC GCGGCCGAAG GCGCGGGCCT CGGCCACCAG TTCCTGCGAC ATCTGCAGCG CACCGGCGTG CTGCTGCATC TCGTCGATCT GGCCCCGTTC GACGAAAACG TGGACCCGGT CGCGGAAGCG AAGGCGATCG TCGGCGAACT GCGCAAGTAC GACGAGGCGC TCTACGAGAA GCCGCGCTGG CTCGTCCTCA ACAAGCTCGA CATGGTGCCG GAAGACGAGC GTGAAGCGCG CGTCGCCGAT TTCCTCGCCC GCTTCGAGTG GGACGGCCCG GTGTTCGAGA TTTCGGCGCT GACGGGCCAG GGCTGCGAAG CGCTGTGCTA CGCGATCTAT GATTACCTCG CCGAGCATTC GGACGCGCAT CGCGCGGCGG AAGAGGAAGA TCTGGCAACG GACGTGCGGT TCCGCGACGC GCCGCCCGCC GATGGCGGGG CGACGCCCGG CGGCGACGCC
Details for BuamA.00120.a.A1.GE32128
HARVESTED ON: 4/26/2011
SEQUENCED ON: 5/2/2011
EXPECTED MW: 41kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MKFIDEARIE VIAGDGGDGS ASMRREKFVP FGGPDGGDGG RGGNVYAIAD RNINTLIDYR YAKKHLARNG ENGRGSDCYG KGGDDVTLRM PVGTVVTDMD TGELIADLTE HGQQVMLAQG GAGGLGNLHF KSSTNRAPRQ KTDGKPGERR MLRLELKVLA DVGLLGMPNA GKSTFISSVS NAKPKIADYP FTTLAPNLGV VRVGPSKSFV IADIPGLIEG AAEGAGLGHQ FLRHLQRTGV LLHLVDLAPF DENVDPVAEA KAIVGELRKY DEALYEKPRW LVLNKLDMVP EDEREARVAD FLARFEWDGP VFEISALTGQ GCEALCYAIY DYLAEHSDAH RAAEEEDLAT DVRFRDAPPA DGGATPGG
Validated NT Sequence
cgccgccggg cgtcgccccg ccatcggcgg gcggcgcgtc gcggaaccgc acgtccgttg ccagatcttc ctcttccgcc gcgcgatgcg cgtccgaatg ctcggcgagg taatcataga tcgcgtagca cagcgcttcg cagccctggc ccgtcagcgc cgaaatctcg aacaccgggc cgtcccactc gaagcgggcg aggaaatcgg cgacgcgcgc ttcacgctcg tcttccggca ccatgtcgag cttgttgagg acgagccagc gcggcttctc gtagagcgcc tcgtcgtact tgcgcagttc gccgacgatc gccttcgctt ccgcgaccgg gtccacgttt tcgtcgaacg gggccagatc gacgagatgc agcagcacgc cggtgcgctg cagatgtcgc aggaactggt ggccgaggcc cgcgccttcg gccgcgcctt cgatcagccc cgggatgtcg gcgatcacga agctcttgct cgggccgacc cgcacgacgc cgaggttcgg cgcgagcgtc gtaaacggat agtcggcgat cttcggcttc gcgttcgaca ccgacgagat gaaggtcgac ttgccggcgt tcggcatgcc gagcagcccg acgtcggcga gcaccttcag ctcgagccgc agcatgcgcc gttcgcccgg cttgccgtcg gtcttctggc gcggcgcacg gttcgtgctc gacttgaaat gcaggttgcc gaggccgccc gcaccgcctt gcgcaagcat cacttgctgg ccgtgctcgg tcaggtcggc gatcagctcg ccggtatcca tgtcggtcac gaccgtgccg accggcatgc gcagcgtgac gtcgtcaccg cccttgccgt agcaatccga gccgcgaccg ttttcgccgt tgcgcgccag gtgcttcttc gcgtaacggt agtcgatcag cgtgttgatg ttgcggtcgg caatcgcgta gacgttgccg ccccggccgc cgtcgccgcc gtccggcccg ccgaacggaa cgaacttttc gcggcgcatc gacgcgctcc catcacctcc gtcgccggcg atgacttcga ttcgtgcttc gtcaatgaac ttcatcgaac caggaccctg ggtctgagct tccagggta
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKFIDEARI EVIAGDGGDG SASMRREKFV PFGGPDGGDG GRGGNVYAIA DRNINTLIDY RYAKKHLARN GENGRGSDCY GKGGDDVTLR MPVGTVVTDM DTGELIADLT EHGQQVMLAQ GGAGGLGNLH FKSSTNRAPR QKTDGKPGER RMLRLELKVL ADVGLLGMPN AGKSTFISSV SNAKPKIADY PFTTLAPNLG VVRVGPSKSF VIADIPGLIE GAAEGAGLGH QFLRHLQRTG VLLHLVDLAP FDENVDPVAE AKAIVGELRK YDEALYEKPR WLVLNKLDMV PEDEREARVA DFLARFEWDG PVFEISALTG QGCEALCYAI YDYLAEHSDA HRAAEEEDLA TDVRFRDAPP ADGGATPGGD A
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaagttcatt gacgaagcac gaatcgaagt catcgccggc gacggaggtg atgggagcgc gtcgatgcgc cgcgaaaagt tcgttccgtt cggcgggccg gacggcggcg acggcggccg gggcggcaac gtctacgcga ttgccgaccg caacatcaac acgctgatcg actaccgtta cgcgaagaag cacctggcgc gcaacggcga aaacggtcgc ggctcggatt gctacggcaa gggcggtgac gacgtcacgc tgcgcatgcc ggtcggcacg gtcgtgaccg acatggatac cggcgagctg atcgccgacc tgaccgagca cggccagcaa gtgatgcttg cgcaaggcgg tgcgggcggc ctcggcaacc tgcatttcaa gtcgagcacg aaccgtgcgc cgcgccagaa gaccgacggc aagccgggcg aacggcgcat gctgcggctc gagctgaagg tgctcgccga cgtcgggctg ctcggcatgc cgaacgccgg caagtcgacc ttcatctcgt cggtgtcgaa cgcgaagccg aagatcgccg actatccgtt tacgacgctc gcgccgaacc tcggcgtcgt gcgggtcggc ccgagcaaga gcttcgtgat cgccgacatc ccggggctga tcgaaggcgc ggccgaaggc gcgggcctcg gccaccagtt cctgcgacat ctgcagcgca ccggcgtgct gctgcatctc gtcgatctgg ccccgttcga cgaaaacgtg gacccggtcg cggaagcgaa ggcgatcgtc ggcgaactgc gcaagtacga cgaggcgctc tacgagaagc cgcgctggct cgtcctcaac aagctcgaca tggtgccgga agacgagcgt gaagcgcgcg tcgccgattt cctcgcccgc ttcgagtggg acggcccggt gttcgagatt tcggcgctga cgggccaggg ctgcgaagcg ctgtgctacg cgatctatga ttacctcgcc gagcattcgg acgcgcatcg cgcggcggaa gaggaagatc tggcaacgga cgtgcggttc cgcgacgcgc cgcccgccga tggcggggcg acgcccggcg gcgacgccta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for BuamA.00120.a.A1.PS01122
PURIFICATION DATe: 6/20/2011
CONCENTRATION: 74.65mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Protocol Notes
notes unavailable
Validated AA Sequence
MKFIDEARIE VIAGDGGDGS ASMRREKFVP FGGPDGGDGG RGGNVYAIAD RNINTLIDYR YAKKHLARNG ENGRGSDCYG KGGDDVTLRM PVGTVVTDMD TGELIADLTE HGQQVMLAQG GAGGLGNLHF KSSTNRAPRQ KTDGKPGERR MLRLELKVLA DVGLLGMPNA GKSTFISSVS NAKPKIADYP FTTLAPNLGV VRVGPSKSFV IADIPGLIEG AAEGAGLGHQ FLRHLQRTGV LLHLVDLAPF DENVDPVAEA KAIVGELRKY DEALYEKPRW LVLNKLDMVP EDEREARVAD FLARFEWDGP VFEISALTGQ GCEALCYAIY DYLAEHSDAH RAAEEEDLAT DVRFRDAPPA DGGATPGG
Validated NT Sequence
cgccgccggg cgtcgccccg ccatcggcgg gcggcgcgtc gcggaaccgc acgtccgttg ccagatcttc ctcttccgcc gcgcgatgcg cgtccgaatg ctcggcgagg taatcataga tcgcgtagca cagcgcttcg cagccctggc ccgtcagcgc cgaaatctcg aacaccgggc cgtcccactc gaagcgggcg aggaaatcgg cgacgcgcgc ttcacgctcg tcttccggca ccatgtcgag cttgttgagg acgagccagc gcggcttctc gtagagcgcc tcgtcgtact tgcgcagttc gccgacgatc gccttcgctt ccgcgaccgg gtccacgttt tcgtcgaacg gggccagatc gacgagatgc agcagcacgc cggtgcgctg cagatgtcgc aggaactggt ggccgaggcc cgcgccttcg gccgcgcctt cgatcagccc cgggatgtcg gcgatcacga agctcttgct cgggccgacc cgcacgacgc cgaggttcgg cgcgagcgtc gtaaacggat agtcggcgat cttcggcttc gcgttcgaca ccgacgagat gaaggtcgac ttgccggcgt tcggcatgcc gagcagcccg acgtcggcga gcaccttcag ctcgagccgc agcatgcgcc gttcgcccgg cttgccgtcg gtcttctggc gcggcgcacg gttcgtgctc gacttgaaat gcaggttgcc gaggccgccc gcaccgcctt gcgcaagcat cacttgctgg ccgtgctcgg tcaggtcggc gatcagctcg ccggtatcca tgtcggtcac gaccgtgccg accggcatgc gcagcgtgac gtcgtcaccg cccttgccgt agcaatccga gccgcgaccg ttttcgccgt tgcgcgccag gtgcttcttc gcgtaacggt agtcgatcag cgtgttgatg ttgcggtcgg caatcgcgta gacgttgccg ccccggccgc cgtcgccgcc gtccggcccg ccgaacggaa cgaacttttc gcggcgcatc gacgcgctcc catcacctcc gtcgccggcg atgacttcga ttcgtgcttc gtcaatgaac ttcatcgaac caggaccctg ggtctgagct tccagggta
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKFIDEARI EVIAGDGGDG SASMRREKFV PFGGPDGGDG GRGGNVYAIA DRNINTLIDY RYAKKHLARN GENGRGSDCY GKGGDDVTLR MPVGTVVTDM DTGELIADLT EHGQQVMLAQ GGAGGLGNLH FKSSTNRAPR QKTDGKPGER RMLRLELKVL ADVGLLGMPN AGKSTFISSV SNAKPKIADY PFTTLAPNLG VVRVGPSKSF VIADIPGLIE GAAEGAGLGH QFLRHLQRTG VLLHLVDLAP FDENVDPVAE AKAIVGELRK YDEALYEKPR WLVLNKLDMV PEDEREARVA DFLARFEWDG PVFEISALTG QGCEALCYAI YDYLAEHSDA HRAAEEEDLA TDVRFRDAPP ADGGATPGGD A
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAAGTTCATT GACGAAGCAC GAATCGAAGT CATCGCCGGC GACGGAGGTG ATGGGAGCGC GTCGATGCGC CGCGAAAAGT TCGTTCCGTT CGGCGGGCCG GACGGCGGCG ACGGCGGCCG GGGCGGCAAC GTCTACGCGA TTGCCGACCG CAACATCAAC ACGCTGATCG ACTACCGTTA CGCGAAGAAG CACCTGGCGC GCAACGGCGA AAACGGTCGC GGCTCGGATT GCTACGGCAA GGGCGGTGAC GACGTCACGC TGCGCATGCC GGTCGGCACG GTCGTGACCG ACATGGATAC CGGCGAGCTG ATCGCCGACC TGACCGAGCA CGGCCAGCAA GTGATGCTTG CGCAAGGCGG TGCGGGCGGC CTCGGCAACC TGCATTTCAA GTCGAGCACG AACCGTGCGC CGCGCCAGAA GACCGACGGC AAGCCGGGCG AACGGCGCAT GCTGCGGCTC GAGCTGAAGG TGCTCGCCGA CGTCGGGCTG CTCGGCATGC CGAACGCCGG CAAGTCGACC TTCATCTCGT CGGTGTCGAA CGCGAAGCCG AAGATCGCCG ACTATCCGTT TACGACGCTC GCGCCGAACC TCGGCGTCGT GCGGGTCGGC CCGAGCAAGA GCTTCGTGAT CGCCGACATC CCGGGGCTGA TCGAAGGCGC GGCCGAAGGC GCGGGCCTCG GCCACCAGTT CCTGCGACAT CTGCAGCGCA CCGGCGTGCT GCTGCATCTC GTCGATCTGG CCCCGTTCGA CGAAAACGTG GACCCGGTCG CGGAAGCGAA GGCGATCGTC GGCGAACTGC GCAAGTACGA CGAGGCGCTC TACGAGAAGC CGCGCTGGCT CGTCCTCAAC AAGCTCGACA TGGTGCCGGA AGACGAGCGT GAAGCGCGCG TCGCCGATTT CCTCGCCCGC TTCGAGTGGG ACGGCCCGGT GTTCGAGATT TCGGCGCTGA CGGGCCAGGG CTGCGAAGCG CTGTGCTACG CGATCTATGA TTACCTCGCC GAGCATTCGG ACGCGCATCG CGCGGCGGAA GAGGAAGATC TGGCAACGGA CGTGCGGTTC CGCGACGCGC CGCCCGCCGA TGGCGGGGCG ACGCCCGGCG GCGACGCCTA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A